Restriction map for
pFN21AB3512
(Circular) (Six-base) MAPSORT of: pFN21AB3512.seq Check: 7091 from: 1 to: 5947
pFN21AB3512 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4010 5394 5662 1632
Size: 2378 1384 268 1917
Fragments arranged by size:
2378 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2420 820
Size: 197 1403 4347
Fragments arranged by size:
4347 1403 197
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 2751
Size: 32 5915
AlwNI CAG_nnn'CTG
Cuts at: 2201 2362 3690 5262 2201
Size: 161 1328 1572 2886
Fragments arranged by size:
2886 1572 1328 161
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2181 3256 3482 3634 5743 1493
Size: 688 1075 226 152 2109 1697
Fragments arranged by size:
2109 1697 1075 688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2958 4501 160
Size: 872 1926 1543 1606
Fragments arranged by size:
1926 1606 1543 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 3266 1391
Size: 658 1217 4072
Fragments arranged by size:
4072 1217 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 2656 3222 928
Size: 971 174 583 566 3653
Fragments arranged by size:
3653 971 583 566 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 2713 2748 1548
Size: 1165 35 4747
Fragments arranged by size:
4747 1165 35
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2972 2984 4558
Size: 107 122 71 631 605 1300 12 1574
Cuts at: 4558 136
Size: 1525
Fragments arranged by size:
1574 1525 1300 631 605 122 107 71
12
BglII A'GATC_T
Cuts at: 2416 5942 2416
Size: 3526 2421
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 4008 5164 1790
Size: 2218 1156 2573
Fragments arranged by size:
2573 2218 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 3678 4636
Size: 416 53 83 399 24 681 1580 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1580 958 681 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4607 1239
Size: 3368 2579
Bpu10I CC'TnA_GC
Cuts at: 2790 2790
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3196 4072 4937 5235 3196
Size: 876 865 298 3908
Fragments arranged by size:
3908 876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4610 882
Size: 617 840 2271 2219
Fragments arranged by size:
2271 2219 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 3166 5941 3166
Size: 2775 3172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5947
BseYI C'CCAG_C
Cuts at: 2205 3237 5150 5646 2205
Size: 1032 1913 496 2506
Fragments arranged by size:
2506 1913 1032 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2423 3165 1163
Size: 573 687 742 3945
Fragments arranged by size:
3945 742 687 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4008 4093 5164 4008
Size: 85 1071 4791
Fragments arranged by size:
4791 1071 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 3266 4008 4093 5164 1391
Size: 399 259 1217 742 85 1071 2174
Fragments arranged by size:
2174 1217 1071 742 399 259 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 3302 844
Size: 1551 907 3489
Fragments arranged by size:
3489 1551 907
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 2597 96
Size: 2501 3446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3173 794
Size: 2379 3568
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 2023 1184
Size: 839 5108
BstYI r'GATC_y
Cuts at: 1972 2416 3277 3644 4029 4046 5498 5584 5596
Size: 444 861 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 861 444 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2091 2339 513
Size: 552 1026 248 4121
Fragments arranged by size:
4121 1026 552 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 3193 3463 4257 4277 2289
Size: 904 270 794 20 3959
Fragments arranged by size:
3959 904 794 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3877 1353
Size: 2524 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4010 4607 5394 5662 1239
Size: 393 2378 597 787 268 1524
Fragments arranged by size:
2378 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2359 2685 2359
Size: 326 5621
EcoRI G'AATT_C
Cuts at: 2181 3256 3634 2181
Size: 1075 378 4494
Fragments arranged by size:
4494 1075 378
FspI TGC'GCA
Cuts at: 2724 4453 2724
Size: 1729 4218
HaeII r_GCGC'y
Cuts at: 1678 2596 5094 1678
Size: 918 2498 2531
Fragments arranged by size:
2531 2498 918
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 3212 3244 4588
Size: 32 135 32 689 32 919 32 1344
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 919 689 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 3291 3537 669
Size: 988 281 292 1061 246 3079
Fragments arranged by size:
3079 1061 988 292 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3150 3546 3150
Size: 396 5551
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3905 4264 4423 518
Size: 648 2739 359 159 2042
Fragments arranged by size:
2739 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2339 513
Size: 552 1274 4121
Fragments arranged by size:
4121 1274 552
NdeI CA'TA_TG
Cuts at: 387 2502 387
Size: 2115 3832
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2192 3022 3082 3305 4850 1132
Size: 97 963 830 60 223 1545 2229
Fragments arranged by size:
2229 1545 963 830 223 97 60
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2201 1108
Size: 1093 4854
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2131 2993 3295 4430 5111 5302 826
Size: 219 1086 862 302 1135 681 191 1471
Fragments arranged by size:
1471 1135 1086 862 681 302 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3211 4051 4952 5214 820
Size: 197 926 477 791 840 901 262 1553
Fragments arranged by size:
1553 926 901 840 791 477 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 2192 3305 1132
Size: 1060 1113 3774
Fragments arranged by size:
3774 1113 1060
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 3101 1517
Size: 1584 4363
StyI C'CwwG_G
Cuts at: 513 1065 2339 3070 3386 513
Size: 552 1274 731 316 3074
Fragments arranged by size:
3074 1274 731 552 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 3974 3991 4144 4329 4774 325
Size: 1255 1335 1059 17 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2597 4193 5905
Size: 274 80 33 51 1443 620 1596 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1712 1596 1443 620 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2848 2880 5907 5939 2202
Size: 32 614 32 3027 32 2210
Fragments arranged by size:
3027 2210 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2202 2202
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2016 2181 4076 2016
Size: 165 1895 3887
Fragments arranged by size:
3887 1895 165
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI