Restriction map for
pFN21AB3511
(Circular) (Six-base) MAPSORT of: pFN21AB3511.seq Check: 5599 from: 1 to: 6106
pFN21AB3511 6106 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2895 3916 278
Size: 53 83 186 936 1359 1021 2468
Fragments arranged by size:
2468 1359 1021 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3332 4169 5553 5821 1632
Size: 1700 837 1384 268 1917
Fragments arranged by size:
1917 1700 1384 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3398 1184
Size: 2214 3892
AlwNI CAG_nnn'CTG
Cuts at: 2396 2840 2869 3849 5421 2396
Size: 444 29 980 1572 3081
Fragments arranged by size:
3081 1572 980 444 29
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2279 2828 3415 3641 3793 5902 1493
Size: 786 549 587 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 587 549 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 3431 1870
Size: 73 1488 4545
Fragments arranged by size:
4545 1488 73
BamHI G'GATC_C
Cuts at: 2384 3436 3803 2384
Size: 1052 367 4687
Fragments arranged by size:
4687 1052 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2311 2524 2617 3427
Size: 325 217 627 513 11 213 93 810
Cuts at: 3427 4882 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 810 627 513 325 217 213
93 11
BanII G_rGCy'C
Cuts at: 1391 2082 2369 2799 3425 1391
Size: 691 287 430 626 4072
Fragments arranged by size:
4072 691 626 430 287
BbeI G_GCGC'C
Cuts at: 2528 2528
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BbvCI CC'TCA_GC
Cuts at: 2355 2355
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3053 3087 4297 4331 1903
Size: 34 1116 34 1210 34 3678
Fragments arranged by size:
3678 1210 1116 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3114 3270 6101 3114
Size: 156 2831 3119
Fragments arranged by size:
3119 2831 156
BlpI GC'TnA_GC
Cuts at: 3306 3523 3306
Size: 217 5889
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2989 4167 5323 1790
Size: 526 673 1178 1156 2573
Fragments arranged by size:
2573 1178 1156 673 526
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 3837 4795
Size: 416 53 83 399 24 901 1519 958
Cuts at: 4795 442
Size: 1753
Fragments arranged by size:
1753 1519 958 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2757
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 2628 2837 2936 4766 1239
Size: 1379 10 209 99 1830 2579
Fragments arranged by size:
2579 1830 1379 209 99 10
Bpu10I CC'TnA_GC
Cuts at: 2355 2841 3059 2355
Size: 486 218 5402
Fragments arranged by size:
5402 486 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4231 5096 5394 4231
Size: 865 298 4943
Fragments arranged by size:
4943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2525 2892 3913 4295
Size: 53 83 186 936 992 367 1021 382
Cuts at: 4295 4930 275
Size: 635 1451
Fragments arranged by size:
1451 1021 992 936 635 382 367 186
83 53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 1178
Size: 30 165 30 1158 30 4693
Fragments arranged by size:
4693 1158 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 1186
Size: 1621 4485
BseYI C'CCAG_C
Cuts at: 2230 2247 3371 5309 5805 2230
Size: 17 1124 1938 496 2531
Fragments arranged by size:
2531 1938 1124 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 2799 4167 4252 5323 2263
Size: 536 1368 85 1071 3046
Fragments arranged by size:
3046 1368 1071 536 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2263 2316 2369 2799 2989 3425
Size: 399 292 181 53 53 430 190 436
Cuts at: 3425 4167 4252 5323 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 430 399 292 190
181 85 53 53
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2812 3461 844
Size: 1968 649 3489
Fragments arranged by size:
3489 1968 649
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4601 4775 65
Size: 1183 1821 1532 174 1396
Fragments arranged by size:
1821 1532 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3358 794
Size: 2564 3542
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 2238 2652 2238
Size: 414 5692
BstYI r'GATC_y
Cuts at: 1972 2384 3114 3270 3436 3803 4188 4205 5657
Size: 412 730 156 166 367 385 17 1452
Cuts at: 5657 5743 5755 6101 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 730 412 385 367 346 166
156 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2742 2742
Size: 6106
BtgI C'CryG_G
Cuts at: 513 1065 2004 2520 2701 513
Size: 552 939 516 181 3918
Fragments arranged by size:
3918 939 552 516 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2554 2580 3262 3622 4416 4436 2554
Size: 26 682 360 794 20 4224
Fragments arranged by size:
4224 794 682 360 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3140 3553 4442
Size: 54 1425 50 275 18 1310 413 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1310 889 413 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2762 4036 1353
Size: 798 611 1274 3423
Fragments arranged by size:
3423 1274 798 611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3251 4693 5067 5213 387
Size: 1064 138 1662 1442 374 146 1280
Fragments arranged by size:
1662 1442 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2797 2797
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2628 2837 2936 3332 4169 4766
Size: 393 986 10 209 99 396 837 597
Cuts at: 4766 5553 5821 1239
Size: 787 268 1524
Fragments arranged by size:
1524 986 837 787 597 396 393 268
209 99 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 6106
EcoO109I rG'GnC_Cy
Cuts at: 2767 2774 2837 2767
Size: 7 63 6036
Fragments arranged by size:
6036 63 7
EcoRI G'AATT_C
Cuts at: 2279 3415 3793 2279
Size: 1136 378 4592
Fragments arranged by size:
4592 1136 378
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2528 5253 1678
Size: 850 2725 2531
Fragments arranged by size:
2725 2531 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2647 2679 2895 2927 4747
Size: 32 135 32 1075 32 216 32 1820
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1820 1075 216 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2524 2524
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 2542 3705 2542
Size: 1163 4943
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3142 10
Size: 54 1425 343 1310 2974
Fragments arranged by size:
2974 1425 1310 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 4064 4423 4582 518
Size: 648 1563 1335 359 159 2042
Fragments arranged by size:
2042 1563 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2925 3310 3319 3522 4199 5347 5592
Size: 645 674 385 9 203 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 674 645 385 245 203
9
NarI GG'CG_CC
Cuts at: 2525 2525
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 2520 513
Size: 552 939 516 4099
Fragments arranged by size:
4099 939 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2132 3379 3464 5009 1132
Size: 97 903 1247 85 1545 2229
Fragments arranged by size:
2229 1545 1247 903 97 85
PasI CC'CwG_GG
Cuts at: 1348 2075 2362 2403 1348
Size: 727 287 41 5051
Fragments arranged by size:
5051 727 287 41
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PfoI T'CCnGG_A
Cuts at: 2481 2913 2481
Size: 432 5674
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 2774 2837 2767
Size: 7 63 6036
Fragments arranged by size:
6036 63 7
PshAI GACnn'nnGTC
Cuts at: 1613 2772 1613
Size: 1159 4947
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 2564 3270 3458 830
Size: 1734 706 188 3478
Fragments arranged by size:
3478 1734 706 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2251 3310 3319 2251
Size: 1059 9 5038
Fragments arranged by size:
5038 1059 9
SacI G_AGCT'C
Cuts at: 2799 2799
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SanDI GG'GwC_CC
Cuts at: 2774 2774
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2187 2560 2635 3266 3454 4589 5270
Size: 219 1142 373 75 631 188 1135 681
Cuts at: 5270 5461 826
Size: 191 1471
Fragments arranged by size:
1471 1142 1135 681 631 373 219 191
188 75
SfoI GGC'GCC
Cuts at: 2526 2526
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4210 5111 5373 820
Size: 197 926 2267 901 262 1553
Fragments arranged by size:
2267 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3379 3464 1132
Size: 2247 85 3774
Fragments arranged by size:
3774 2247 85
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 1517
Size: 663 5443
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 2520 3545 513
Size: 552 939 300 216 1025 3074
Fragments arranged by size:
3074 1025 939 552 300 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 4352 6064
Size: 274 80 33 51 1443 990 1385 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1712 1443 1385 990 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2561 2593 6066 6098 2561
Size: 32 3473 32 2569
Fragments arranged by size:
3473 2569 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2060
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 2870 4235 2870
Size: 1365 4741
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2893 3914 276
Size: 53 83 186 936 1359 1021 2468
Fragments arranged by size:
2468 1359 1021 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI