Restriction map for
pFN21AB3507
(Circular) (Six-base) MAPSORT of: pFN21AB3507.seq Check: 3490 from: 1 to: 5824
pFN21AB3507 5824 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 2038
Size: 5824
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2902 3634 278
Size: 53 83 186 936 1366 732 2468
Fragments arranged by size:
2468 1366 936 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2414 3167 1937
Size: 477 753 4594
Fragments arranged by size:
4594 753 477
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2755 2825 3887 5271 5539 1632
Size: 1123 70 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1123 1062 268 70
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2055 2232 3077 4723 5700 1203
Size: 852 177 845 1646 977 1327
Fragments arranged by size:
1646 1327 977 852 845 177
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2859 1166
Size: 1693 4131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2475 2475
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2185 2612 3133 3359 3511 5620 1493
Size: 692 427 521 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 521 427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3149 1870
Size: 73 1206 4545
Fragments arranged by size:
4545 1206 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2436 2844 3145 4600 618
Size: 325 217 627 649 408 301 1455 1842
Fragments arranged by size:
1842 1455 649 627 408 325 301 217
BanII G_rGCy'C
Cuts at: 1391 2157 2475 3108 3143 1391
Size: 766 318 633 35 4072
Fragments arranged by size:
4072 766 633 318 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 3074 928
Size: 971 801 374 3678
Fragments arranged by size:
3678 971 801 374
BbvCI CC'TCA_GC
Cuts at: 2014 2014
Size: 5824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2509 2543 2924 2958 4015 4049 1903
Size: 34 572 34 381 34 1057 34 3678
Fragments arranged by size:
3678 1057 572 381 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2439 2475 3885 5041 1790
Size: 649 36 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 649 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2599 3555 4513
Size: 416 53 83 399 24 1182 956 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1182 958 956 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2672
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2182 2583 4484 1239
Size: 894 49 401 1901 2579
Fragments arranged by size:
2579 1901 894 401 49
Bpu10I CC'TnA_GC
Cuts at: 2014 2724 2014
Size: 710 5114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2327 4487 882
Size: 617 828 2160 2219
Fragments arranged by size:
2219 2160 828 617
BsaAI yAC'GTr
Cuts at: 493 1204 3078 3615 493
Size: 711 1874 537 2702
Fragments arranged by size:
2702 1874 711 537
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2899 3631 4013 4648
Size: 53 83 186 936 1366 732 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1366 936 732 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4576
Fragments arranged by size:
4576 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 2831 1186
Size: 1621 24 4179
Fragments arranged by size:
4179 1621 24
BseYI C'CCAG_C
Cuts at: 5027 5523 5027
Size: 496 5328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2772 1163
Size: 573 1036 4215
Fragments arranged by size:
4215 1036 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3885 3970 5041 3885
Size: 85 1071 4668
Fragments arranged by size:
4668 1071 85
BsmI GAATG_Cn'
Cuts at: 2976 3335 3428 2976
Size: 359 93 5372
Fragments arranged by size:
5372 359 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2439 2475 3108 3143 3885 3970
Size: 399 367 282 36 633 35 742 85
Cuts at: 3970 5041 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 633 399 367 282 85
36 35
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 3179 844
Size: 1194 1141 3489
Fragments arranged by size:
3489 1194 1141
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3095 4468 4655 1700
Size: 110 1285 1373 187 2869
Fragments arranged by size:
2869 1373 1285 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 3868
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 2240 3884 4896 2240
Size: 1644 1012 3168
Fragments arranged by size:
3168 1644 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2164
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2415 2415
Size: 5824
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3114 487
Size: 683 56 1888 3197
Fragments arranged by size:
3197 1888 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 2103 1554
Size: 549 5275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 3271 4160
Size: 54 1425 50 275 18 1162 279 889
Cuts at: 4160 5789 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1162 889 279 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 3754 1353
Size: 810 1591 3423
Fragments arranged by size:
3423 1591 810
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2182 2583 2755 2825 3887 4484
Size: 393 501 49 401 172 70 1062 597
Cuts at: 4484 5271 5539 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1062 787 597 501 401 393 268
172 70 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2518 2631 2066
Size: 452 113 5259
Fragments arranged by size:
5259 452 113
EcoO109I rG'GnC_Cy
Cuts at: 2263 2278 2471 2472 2263
Size: 15 193 1 5615
Fragments arranged by size:
5615 193 15 1
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2967 4971 1678
Size: 1289 2004 2531
Fragments arranged by size:
2531 2004 1289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373
Size: 32 135 32 2893 32 42 32 2626
Fragments arranged by size:
2893 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2481 2949 4938 5122 1305
Size: 942 234 468 1989 184 2007
Fragments arranged by size:
2007 1989 942 468 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2994 10
Size: 54 1425 343 1162 2840
Fragments arranged by size:
2840 1425 1162 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2859 3782 4141 4300 518
Size: 648 1693 923 359 159 2042
Fragments arranged by size:
2042 1693 923 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3240 3917 5065 5310 1606
Size: 1634 677 1148 245 2120
Fragments arranged by size:
2120 1634 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2059 2236 3182 4727 1132
Size: 97 830 177 946 1545 2229
Fragments arranged by size:
2229 1545 946 830 177 97
PasI CC'CwG_GG
Cuts at: 1348 2468 2476 1348
Size: 1120 8 4696
Fragments arranged by size:
4696 1120 8
PciI A'CATG_T
Cuts at: 2055 2232 4723 2055
Size: 177 2491 3156
Fragments arranged by size:
3156 2491 177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 3078 3078
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 2278 2263
Size: 15 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2471 2471
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2332 3176 830
Size: 1502 844 3478
Fragments arranged by size:
3478 1502 844
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2263 2263
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 2203 4072 5784 2203
Size: 1869 1712 2243
Fragments arranged by size:
2243 1869 1712
SfcI C'TryA_G
Cuts at: 826 1045 2328 3172 4307 4988 5179 826
Size: 219 1283 844 1135 681 191 1471
Fragments arranged by size:
1471 1283 1135 844 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2326 2883 1517
Size: 809 557 4458
Fragments arranged by size:
4458 809 557
StyI C'CwwG_G
Cuts at: 513 1065 2778 2874 3263 513
Size: 552 1713 96 389 3074
Fragments arranged by size:
3074 1713 552 389 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 3851 3868 4021 4206 4651 325
Size: 1255 1070 1201 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1201 1070 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2201 2313
Size: 274 80 33 51 1443 75 149 112
Cuts at: 2313 2808 3114 4070 5782 96
Size: 495 306 956 1712 138
Fragments arranged by size:
1712 1443 956 495 306 274 149 138
112 80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 2881
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2900 3632 276
Size: 53 83 186 936 1366 732 2468
Fragments arranged by size:
2468 1366 936 732 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI