Restriction map for
pFN21AB3505
(Circular) (Six-base) MAPSORT of: pFN21AB3505.seq Check: 6534 from: 1 to: 5767
pFN21AB3505 5767 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 3110 1937
Size: 286 887 4594
Fragments arranged by size:
4594 887 286
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2730 3830 5214 5482 1632
Size: 735 363 1100 1384 268 1917
Fragments arranged by size:
1917 1384 1100 735 363 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2975 4666 5643 1203
Size: 1772 1691 977 1327
Fragments arranged by size:
1772 1691 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 2433 3092 1870
Size: 73 358 132 659 4545
Fragments arranged by size:
4545 659 358 132 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2849 3086 1391
Size: 1458 237 4072
Fragments arranged by size:
4072 1458 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 928
Size: 971 1035 3761
Fragments arranged by size:
3761 1035 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2734 2734
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2581 2618 5762 2581
Size: 37 3144 2586
Fragments arranged by size:
3144 2586 37
BlpI GC'TnA_GC
Cuts at: 2018 3184 2018
Size: 1166 4601
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2336 1056
Size: 1280 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 2397 4427 1239
Size: 1119 39 2030 2579
Fragments arranged by size:
2579 2030 1119 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3065 4430 882
Size: 617 1566 1365 2219
Fragments arranged by size:
2219 1566 1365 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4743
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1996 3020 1163
Size: 573 260 1024 3910
Fragments arranged by size:
3910 1024 573 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3217 3978 5006 1540
Size: 275 186 64 1152 761 1028 2301
Fragments arranged by size:
2301 1152 1028 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 2736 3278 3371 2736
Size: 542 93 5132
Fragments arranged by size:
5132 542 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2849 3086 3828 3913 4984 1391
Size: 399 1059 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 1059 742 399 237 85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2626 3654 1394
Size: 429 224 579 1028 3507
Fragments arranged by size:
3507 1028 579 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 2641 4262 4436 65
Size: 1183 1393 1621 174 1396
Fragments arranged by size:
1621 1396 1393 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 2410 96
Size: 2314 3453
BssHII G'CGCG_C
Cuts at: 1925 2096 1925
Size: 171 5596
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 794
Size: 1578 4189
BstBI TT'CG_AA
Cuts at: 2649 4571 2649
Size: 1922 3845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2180
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2429 2581 2618 2818 3097 3464 3849 3866
Size: 457 152 37 200 279 367 385 17
Cuts at: 3866 5318 5404 5416 5762 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 457 385 367 346 279 200
152 86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 3283 4077 4097 2223
Size: 1060 794 20 3893
Fragments arranged by size:
3893 1060 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 2593 3071 2593
Size: 478 5289
DraIII CAC_nnn'GTG
Cuts at: 2687 2687
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732
Size: 54 1425 50 275 18 1384 889 1629
Cuts at: 5732 8
Size: 43
Fragments arranged by size:
1629 1425 1384 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2889 3697 1353
Size: 834 702 808 3423
Fragments arranged by size:
3423 834 808 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2321 4354 4728 4874 387
Size: 1064 138 732 2033 374 146 1280
Fragments arranged by size:
2033 1280 1064 732 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 2367 2397 2730 3830 4427 5214
Size: 393 726 9 30 333 1100 597 787
Cuts at: 5214 5482 1239
Size: 268 1524
Fragments arranged by size:
1524 1100 787 726 597 393 333 268
30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2839 2302
Size: 537 5230
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2025 4914 1678
Size: 347 2889 2531
Fragments arranged by size:
2889 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373
Size: 32 135 32 2836 32 42 32 2626
Fragments arranged by size:
2836 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 2148 3366 2148
Size: 1218 4549
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2903 3183 3860 5008 5253 1606
Size: 1297 280 677 1148 245 2120
Fragments arranged by size:
2120 1297 1148 677 280 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
NdeI CA'TA_TG
Cuts at: 387 2762 2890 387
Size: 2375 128 3264
Fragments arranged by size:
3264 2375 128
NheI G'CTAG_C
Cuts at: 1052 2332 1052
Size: 1280 4487
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2736 2736
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2979 3125 4670 1132
Size: 97 1750 146 1545 2229
Fragments arranged by size:
2229 1750 1545 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 2975 4666 2975
Size: 1691 4076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 2384 3337 2384
Size: 953 4814
PstI C_TGCA'G
Cuts at: 830 2368 2461 3119 830
Size: 1538 93 658 3478
Fragments arranged by size:
3478 1538 658 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2903 2903
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SexAI A'CCwGG_T
Cuts at: 2697 2697
Size: 5767
SfcI C'TryA_G
Cuts at: 826 1045 2224 2364 2457 2547 3115 4250 4931
Size: 219 1179 140 93 90 568 1135 681
Cuts at: 4931 5122 826
Size: 191 1471
Fragments arranged by size:
1471 1179 1135 681 568 219 191 140
93 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2303 3094 1872
Size: 431 791 4545
Fragments arranged by size:
4545 791 431
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SrfI GCCC'GGGC
Cuts at: 2303 2303
Size: 5767
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 3206 513
Size: 552 939 1202 3074
Fragments arranged by size:
3074 1202 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2981 3794 3811 3964 4149 4594 325
Size: 1255 1401 813 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 813 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2144 2410 4013
Size: 274 80 33 51 1443 167 266 1603
Cuts at: 4013 5725 96
Size: 1712 138
Fragments arranged by size:
1712 1603 1443 274 266 167 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2389 2421 5727 5759 2389
Size: 32 3306 32 2397
Fragments arranged by size:
3306 2397 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2301 3092 1870
Size: 431 791 4545
Fragments arranged by size:
4545 791 431
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SwaI
XcmI