Restriction map for
pFN21AB3501
(Circular) (Six-base) MAPSORT of: pFN21AB3501.seq Check: 902 from: 1 to: 5845
pFN21AB3501 5845 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2303 2745 3908 5292 5560 1632
Size: 587 84 442 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 587 442 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 2094 2163 2854 4744 5721 1203
Size: 891 69 691 1890 977 1327
Fragments arranged by size:
1890 1327 977 891 691 69
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AlwNI CAG_nnn'CTG
Cuts at: 2768 2868 3091 3588 5160 2768
Size: 100 223 497 1572 3453
Fragments arranged by size:
3453 1572 497 223 100
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2233 2276 2313 2341 3038 3154 3380 3532
Size: 740 43 37 28 697 116 226 152
Cuts at: 3532 5641 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 740 697 226 152 116 43
37 28
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2143 3170 1870
Size: 73 82 118 1027 4545
Fragments arranged by size:
4545 1027 118 82 73
BamHI G'GATC_C
Cuts at: 2511 2736 3175 3542 2511
Size: 225 439 367 4814
Fragments arranged by size:
4814 439 367 225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2149 3166 4621 618
Size: 325 217 627 362 1017 1455 1842
Fragments arranged by size:
1842 1455 1017 627 362 325 217
BanII G_rGCy'C
Cuts at: 1391 3117 3164 1391
Size: 1726 47 4072
Fragments arranged by size:
4072 1726 47
BbeI G_GCGC'C
Cuts at: 2153 2153
Size: 5845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BfrBI ATG'CAT
Cuts at: 3103 3103
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3001 5840 3001
Size: 2839 3006
BlpI GC'TnA_GC
Cuts at: 2057 2900 3262 2057
Size: 843 362 4640
Fragments arranged by size:
4640 843 362
Bme1580I G_kGCm'C
Cuts at: 1790 2226 3906 5062 1790
Size: 436 1680 1156 2573
Fragments arranged by size:
2573 1680 1156 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2095 3636 493
Size: 711 891 1541 2702
Fragments arranged by size:
2702 1541 891 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 3652 4034 4669
Size: 53 83 186 936 617 1502 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1502 1451 936 635 617 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2181 2436 4278 4676 4950 5097 5508 2181
Size: 255 1842 398 274 147 411 2518
Fragments arranged by size:
2518 1842 411 398 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2515 2545 1178
Size: 30 165 30 1112 30 4478
Fragments arranged by size:
4478 1112 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5845
BseYI C'CCAG_C
Cuts at: 2041 2639 5048 5544 2041
Size: 598 2409 496 2342
Fragments arranged by size:
2409 2342 598 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2906 3906 3991 5062 2906
Size: 1000 85 1071 3689
Fragments arranged by size:
3689 1071 1000 85
BsmI GAATG_Cn'
Cuts at: 3105 3356 3449 3105
Size: 251 93 5501
Fragments arranged by size:
5501 251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2906 3117 3164 3906 3991 5062
Size: 399 436 680 211 47 742 85 1071
Cuts at: 5062 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 680 436 399 211 85
47
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3200 844
Size: 2356 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4489 4676 1700
Size: 110 2679 187 2869
Fragments arranged by size:
2869 2679 187 110
BsrGI T'GTAC_A
Cuts at: 96 2160 2919 96
Size: 2064 759 3022
Fragments arranged by size:
3022 2064 759
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2647 2960 2041
Size: 606 313 4926
Fragments arranged by size:
4926 606 313
BstYI r'GATC_y
Cuts at: 1972 2511 2736 2949 3001 3175 3542 3927 3944
Size: 539 225 213 52 174 367 385 17
Cuts at: 3944 5396 5482 5494 5840 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 539 385 367 346 225 213
174 86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5293
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 2709 3149 2709
Size: 440 5405
DraIII CAC_nnn'GTG
Cuts at: 3051 3140 3051
Size: 89 5756
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2834 2969 3292
Size: 54 1425 50 275 18 1004 135 323
Cuts at: 3292 4181 5810 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1004 889 323 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 3775 1353
Size: 1587 835 3423
Fragments arranged by size:
3423 1587 835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2303 2745 3908 4505 5292 5560
Size: 393 587 84 442 1163 597 787 268
Cuts at: 5560 1239
Size: 1524
Fragments arranged by size:
1524 1163 787 597 587 442 393 268
84
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 2105
Size: 5845
EcoRI G'AATT_C
Cuts at: 2233 3154 3532 2233
Size: 921 378 4546
Fragments arranged by size:
4546 921 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2333 2333
Size: 5845
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2153 4992 1678
Size: 475 2839 2531
Fragments arranged by size:
2839 2531 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373
Size: 32 135 32 2914 32 42 32 2626
Fragments arranged by size:
2914 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2788 2860 3189 3435 669
Size: 988 281 850 72 329 246 3079
Fragments arranged by size:
3079 988 850 329 281 246 72
HindIII A'AGCT_T
Cuts at: 748 2714 748
Size: 1966 3879
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KasI G'GCGC_C
Cuts at: 2149 2149
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2479 2587 2848 4959 5143 1305
Size: 1174 108 261 2111 184 2007
Fragments arranged by size:
2111 2007 1174 261 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2409 2958 3018 3803 4162 4321
Size: 648 1162 81 549 60 785 359 159
Cuts at: 4321 518
Size: 2042
Fragments arranged by size:
2042 1162 785 648 549 359 159 81
60
MspA1I CmG'CkG
Cuts at: 1606 3070 3088 3261 3938 5086 5331 1606
Size: 1464 18 173 677 1148 245 2120
Fragments arranged by size:
2120 1464 1148 677 245 173 18
NarI GG'CG_CC
Cuts at: 2150 2150
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NsiI A_TGCA'T
Cuts at: 3105 3105
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2167 2327 2858 3203 4748 1132
Size: 97 938 160 531 345 1545 2229
Fragments arranged by size:
2229 1545 938 531 345 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 2163 2854 4744 2163
Size: 691 1890 3264
Fragments arranged by size:
3264 1890 691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2733 3051 1108
Size: 1625 318 3902
Fragments arranged by size:
3902 1625 318
PfoI T'CCnGG_A
Cuts at: 2646 2646
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 2481 3415 2481
Size: 934 4911
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3025 3057 3025
Size: 32 5813
PstI C_TGCA'G
Cuts at: 830 3197 830
Size: 2367 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 3070 3088 3070
Size: 18 5827
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 3193 4328 5009 5200 826
Size: 219 2148 1135 681 191 1471
Fragments arranged by size:
2148 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2151 2151
Size: 5845
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 2027 2145 3172 1872
Size: 155 118 1027 4545
Fragments arranged by size:
4545 1027 155 118
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 2485 2657 3769 5
Size: 47 2433 172 1112 2081
Fragments arranged by size:
2433 2081 1112 172 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 3284 513
Size: 552 2219 3074
Fragments arranged by size:
3074 2219 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2476 3872 3889 4042 4227 4672
Size: 1255 678 218 1396 17 153 185 445
Cuts at: 4672 325
Size: 1498
Fragments arranged by size:
1498 1396 1255 678 445 218 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2919 4091
Size: 274 80 33 51 1443 183 759 1172
Cuts at: 4091 5803 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 759 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2515 2547 5805 5837 2515
Size: 32 3258 32 2523
Fragments arranged by size:
3258 2523 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3130 1316
Size: 1814 4031
XbaI T'CTAG_A
Cuts at: 2206 3181 2206
Size: 975 4870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 2025 2143 3170 1870
Size: 155 118 1027 4545
Fragments arranged by size:
4545 1027 155 118
XmnI GAAnn'nnTTC
Cuts at: 2117 2316 3003 3974 2117
Size: 199 687 971 3988
Fragments arranged by size:
3988 971 687 199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI