Restriction map for
pFN21AB3493
(Circular) (Six-base) MAPSORT of: pFN21AB3493.seq Check: 1626 from: 1 to: 6595
pFN21AB3493 6595 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2403 2403
Size: 6595
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4405 278
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2172 2970 3938 1937
Size: 235 798 968 4594
Fragments arranged by size:
4594 968 798 235
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3068 4658 6042 6310 1632
Size: 725 711 1590 1384 268 1917
Fragments arranged by size:
1917 1590 1384 725 711 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 5494 6471 1203
Size: 4291 977 1327
Fragments arranged by size:
4291 1327 977
AgeI A'CCGG_T
Cuts at: 5426 5426
Size: 6595
AhdI GACnn_n'nnGTC
Cuts at: 3115 5324 3115
Size: 2209 4386
AleI CACnn'nnGTG
Cuts at: 1166 3731 1166
Size: 2565 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2032 1184
Size: 848 5747
AlwNI CAG_nnn'CTG
Cuts at: 2233 2899 3508 4338 5910 2233
Size: 666 609 830 1572 2918
Fragments arranged by size:
2918 1572 830 666 609
ApaI G_GGCC'C
Cuts at: 3617 3617
Size: 6595
ApaLI G'TGCA_C
Cuts at: 4652 5808 4652
Size: 1156 5439
ApoI r'AATT_y
Cuts at: 1493 2498 3904 4130 4282 6391 1493
Size: 1005 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 1005 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5149 160
Size: 872 4117 1606
Fragments arranged by size:
4117 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2510 2978 3920 1870
Size: 73 307 260 468 942 4545
Fragments arranged by size:
4545 942 468 307 260 73
BamHI G'GATC_C
Cuts at: 3534 3925 4292 3534
Size: 391 367 5837
Fragments arranged by size:
5837 391 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3866 3916 5371 618
Size: 325 217 627 2079 50 1455 1842
Fragments arranged by size:
2079 1842 1455 627 325 217 50
BanII G_rGCy'C
Cuts at: 1391 2061 2141 3078 3229 3360 3617 3914 1391
Size: 670 80 937 151 131 257 297 4072
Fragments arranged by size:
4072 937 670 297 257 151 131 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 928
Size: 971 461 5163
Fragments arranged by size:
5163 971 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4786 4820 1903
Size: 34 2849 34 3678
Fragments arranged by size:
3678 2849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 2318 5206
Size: 107 122 71 631 605 574 72 2888
Cuts at: 5206 136
Size: 1525
Fragments arranged by size:
2888 1525 631 605 574 122 107 72
71
BglII A'GATC_T
Cuts at: 3675 6590 3675
Size: 2915 3680
BlpI GC'TnA_GC
Cuts at: 4012 4012
Size: 6595
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2156 3617 4656 5812 1790
Size: 231 135 1461 1039 1156 2573
Fragments arranged by size:
2573 1461 1156 1039 231 135
BmgBI CAC'GTC
Cuts at: 1318 2124 2358 1318
Size: 806 234 5555
Fragments arranged by size:
5555 806 234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 3553 4326
Size: 416 53 83 399 24 1661 475 773
Cuts at: 4326 5284 442
Size: 958 1753
Fragments arranged by size:
1753 1661 958 773 475 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2618 2948 3053 3120 3347 3383 5255
Size: 836 543 330 105 67 227 36 1872
Cuts at: 5255 1239
Size: 2579
Fragments arranged by size:
2579 1872 836 543 330 227 105 67
36
Bpu10I CC'TnA_GC
Cuts at: 2576 3354 2576
Size: 778 5817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3296 4720 5585 5883 2834
Size: 462 1424 865 298 3546
Fragments arranged by size:
3546 1424 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2765 3157 3323 5258 882
Size: 617 1266 392 166 1935 2219
Fragments arranged by size:
2219 1935 1266 617 392 166
BsaAI yAC'GTr
Cuts at: 493 1204 4386 493
Size: 711 3182 2702
Fragments arranged by size:
3182 2702 711
BsaBI GATnn'nnATC
Cuts at: 6589 6589
Size: 6595
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2276 2651 2763 4402
Size: 53 83 186 936 743 375 112 1639
Cuts at: 4402 4784 5419 275
Size: 382 635 1451
Fragments arranged by size:
1639 1451 936 743 635 382 375 186
112 83 53
BsaWI w'CCGG_w
Cuts at: 5028 5426 5700 5847 6258 5028
Size: 398 274 147 411 5365
Fragments arranged by size:
5365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 3342 3372 3629
Size: 30 165 30 621 30 1288 30 257
Cuts at: 3629 3659 1178
Size: 30 4114
Fragments arranged by size:
4114 1288 621 257 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2816 2837 2897 1186
Size: 859 771 21 60 4884
Fragments arranged by size:
4884 859 771 60 21
BseYI C'CCAG_C
Cuts at: 2300 2478 3112 3203 3508 5798 6294 2300
Size: 178 634 91 305 2290 496 2601
Fragments arranged by size:
2601 2290 634 496 305 178 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2280 3500 1163
Size: 573 441 103 1220 4258
Fragments arranged by size:
4258 1220 573 441 103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2186 3473 3634 4045 4806 5834
Size: 275 186 185 1287 161 411 761 1028
Cuts at: 5834 1540
Size: 2301
Fragments arranged by size:
2301 1287 1028 761 411 275 186 185
161
BsiHKAI G_wGCw'C
Cuts at: 2045 2394 2517 2662 3026 3078 3360 4656 4741
Size: 349 123 145 364 52 282 1296 85
Cuts at: 4741 5812 2045
Size: 1071 2828
Fragments arranged by size:
2828 1296 1071 364 349 282 145 123
85 52
BsmI GAATG_Cn'
Cuts at: 2314 4106 4199 2314
Size: 1792 93 4710
Fragments arranged by size:
4710 1792 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2114 2114
Size: 6595
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2045 2061 2141 2156 2394 2517
Size: 399 231 24 16 80 15 238 123
Cuts at: 2517 2662 3026 3078 3229 3360 3617 3914 4656
Size: 145 364 52 151 131 257 297 742
Cuts at: 4656 4741 5812 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 297 257 238
231 151 145 131 123 85 80 52
24 16 15
BspEI T'CCGG_A
Cuts at: 6258 6258
Size: 6595
BspHI T'CATG_A
Cuts at: 4484 6214 4484
Size: 1730 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3950 844
Size: 1559 1547 3489
Fragments arranged by size:
3489 1559 1547
BsrBI CCG'CTC
Cuts at: 1394 1823 4482 1394
Size: 429 2659 3507
Fragments arranged by size:
3507 2659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5090 5264 65
Size: 1183 3842 174 1396
Fragments arranged by size:
3842 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 3468 3606 5239 5426 1700
Size: 110 261 1397 138 1633 187 2869
Fragments arranged by size:
2869 1633 1397 261 187 138 110
BsrGI T'GTAC_A
Cuts at: 96 2631 96
Size: 2535 4060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 4655 5667 4655
Size: 1012 5583
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2233 2692 794
Size: 1439 459 4697
Fragments arranged by size:
4697 1439 459
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 2074 3092 1184
Size: 890 1018 4687
Fragments arranged by size:
4687 1018 890
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 3325 3733 2053
Size: 1272 408 4915
Fragments arranged by size:
4915 1272 408
BstYI r'GATC_y
Cuts at: 1972 2865 3534 3675 3925 4292 4677 4694 6146
Size: 893 669 141 250 367 385 17 1452
Cuts at: 6146 6232 6244 6590 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 893 669 385 367 346 250
141 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2609 2609
Size: 6595
BtgI C'CryG_G
Cuts at: 513 1065 2004 3757 513
Size: 552 939 1753 3351
Fragments arranged by size:
3351 1753 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 2329 3059 3517 4111 4905 4925 2165
Size: 164 730 458 594 794 20 3835
Fragments arranged by size:
3835 794 730 594 458 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3556 3591 529
Size: 35 2992 35 3533
Fragments arranged by size:
3533 2992 35 35
DraI TTT'AAA
Cuts at: 3249 3899 3249
Size: 650 5945
DraIII CAC_nnn'GTG
Cuts at: 2085 2085
Size: 6595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5602 809
Size: 507 4286 1802
Fragments arranged by size:
4286 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2089 3339
Size: 54 1425 50 275 18 195 64 1250
Cuts at: 3339 3470 3631 4042 4931 6560 8
Size: 131 161 411 889 1629 43
Fragments arranged by size:
1629 1425 1250 889 411 275 195 161
131 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3470 3631 4042 1537
Size: 275 1658 161 411 4090
Fragments arranged by size:
4090 1658 411 275 161
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 2801 2852 4525 1353
Size: 1440 8 51 1673 3423
Fragments arranged by size:
3423 1673 1440 51 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5182 5556 5702 387
Size: 1064 138 3593 374 146 1280
Fragments arranged by size:
3593 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3076 3358 3076
Size: 282 6313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2357 2618 2948 3053 3068 3120
Size: 393 443 282 261 330 105 15 52
Cuts at: 3120 3347 3383 4658 5255 6042 6310 1239
Size: 227 36 1275 597 787 268 1524
Fragments arranged by size:
1524 1275 787 597 443 393 330 282
268 261 227 105 52 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2339 2579 2782 2027
Size: 312 240 203 5840
Fragments arranged by size:
5840 312 240 203
EcoO109I rG'GnC_Cy
Cuts at: 3613 3613
Size: 6595
EcoRI G'AATT_C
Cuts at: 3904 4282 3904
Size: 378 6217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2773 3586 2377
Size: 396 813 5386
Fragments arranged by size:
5386 813 396
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2592 3645 5742 1678
Size: 914 1053 2097 2531
Fragments arranged by size:
2531 2097 1053 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2921 2953 3101
Size: 32 135 32 449 32 868 32 148
Cuts at: 3101 3133 3342 3374 5236 5268 5310 5342 1373
Size: 32 209 32 1862 32 42 32 2626
Fragments arranged by size:
2626 1862 868 449 209 148 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2815 3939 4185 669
Size: 988 281 877 1124 246 3079
Fragments arranged by size:
3079 1124 988 877 281 246
HindIII A'AGCT_T
Cuts at: 748 3209 748
Size: 2461 4134
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 4194 4194
Size: 6595
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5709 5893 1305
Size: 4404 184 2007
Fragments arranged by size:
4404 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3341 10
Size: 54 1425 343 1509 3264
Fragments arranged by size:
3264 1509 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2149 2744 3731 4553 4912 5071 518
Size: 648 983 595 987 822 359 159 2042
Fragments arranged by size:
2042 987 983 822 648 595 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2437 4011 4688 5836 6081 1606
Size: 585 246 1574 677 1148 245 2120
Fragments arranged by size:
2120 1574 1148 677 585 246 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5104
Fragments arranged by size:
5104 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6595
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NruI TCG'CGA
Cuts at: 2454 2454
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 3953 5498 1132
Size: 97 2724 1545 2229
Fragments arranged by size:
2724 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6595
PciI A'CATG_T
Cuts at: 5494 5494
Size: 6595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6595
PfoI T'CCnGG_A
Cuts at: 2424 3069 3360 3384 2424
Size: 645 291 24 5635
Fragments arranged by size:
5635 645 291 24
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4664 4696 6177 6209 4664
Size: 32 1481 32 5050
Fragments arranged by size:
5050 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6595
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PspOMI G'GGCC_C
Cuts at: 3613 3613
Size: 6595
PstI C_TGCA'G
Cuts at: 830 2238 2400 2442 3274 3561 3947 830
Size: 1408 162 42 832 287 386 3478
Fragments arranged by size:
3478 1408 832 386 287 162 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 6595
SacI G_AGCT'C
Cuts at: 3078 3360 3078
Size: 282 6313
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SbfI CC_TGCA'GG
Cuts at: 2238 3947 2238
Size: 1709 4886
ScaI AGT'ACT
Cuts at: 4843 6555 4843
Size: 1712 4883
SfcI C'TryA_G
Cuts at: 826 1045 2234 2396 2438 3270 3557 3943 5078
Size: 219 1189 162 42 832 287 386 1135
Cuts at: 5078 5759 5950 826
Size: 681 191 1471
Fragments arranged by size:
1471 1189 1135 832 681 386 287 219
191 162 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2246 2246
Size: 6595
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 2252 3922 1872
Size: 380 1670 4545
Fragments arranged by size:
4545 1670 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2849 3311 4699 5600 5862 820
Size: 197 926 906 462 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 906 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 3953 1132
Size: 2821 3774
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 2558 1517
Size: 1041 5554
StyI C'CwwG_G
Cuts at: 513 1065 2004 3318 3720 3891 4034 513
Size: 552 939 1314 402 171 143 3074
Fragments arranged by size:
3074 1314 939 552 402 171 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2314 4622 4639 4792 4977 5422 325
Size: 1255 734 2308 17 153 185 445 1498
Fragments arranged by size:
2308 1498 1255 734 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2631 4841 6553
Size: 274 80 33 51 1443 654 2210 1712
Cuts at: 6553 96
Size: 138
Fragments arranged by size:
2210 1712 1443 654 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 6555 6587 2024
Size: 32 4499 32 2032
Fragments arranged by size:
4499 2032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3791 1316
Size: 2475 4120
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2593 2593
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 2250 3920 1870
Size: 380 1670 4545
Fragments arranged by size:
4545 1670 380
XmnI GAAnn'nnTTC
Cuts at: 3569 4724 3569
Size: 1155 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4403 276
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfoI SrfI SwaI