Restriction map for
pFN21AB3491
(Circular) (Six-base) MAPSORT of: pFN21AB3491.seq Check: 7334 from: 1 to: 6739
pFN21AB3491 6739 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3578 3634 3578
Size: 56 6683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4082 1937
Size: 2145 4594
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2829 2909 4802 6186 6454 1632
Size: 1197 80 1893 1384 268 1917
Fragments arranged by size:
1917 1893 1384 1197 268 80
AfeI AGC'GCT
Cuts at: 2129 2129
Size: 6739
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 3547 5638 6615 1203
Size: 2344 2091 977 1327
Fragments arranged by size:
2344 2091 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 3398 5468 3398
Size: 2070 4669
AleI CACnn'nnGTG
Cuts at: 1166 3032 3912 1166
Size: 1866 880 3993
Fragments arranged by size:
3993 1866 880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3666 1184
Size: 2482 4257
AlwNI CAG_nnn'CTG
Cuts at: 2473 2621 3011 4482 6054 2473
Size: 148 390 1471 1572 3158
Fragments arranged by size:
3158 1572 1471 390 148
ApaI G_GGCC'C
Cuts at: 3723 3723
Size: 6739
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2343 2396 2450 2759 3251 3432 3959 3980
Size: 850 53 54 309 492 181 527 21
Cuts at: 3980 4048 4274 4426 6535 1493
Size: 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 850 527 492 309 226 181
152 68 54 53 21
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 3750 4064 1870
Size: 73 1807 314 4545
Fragments arranged by size:
4545 1807 314 73
BamHI G'GATC_C
Cuts at: 2038 4069 4436 2038
Size: 2031 367 4341
Fragments arranged by size:
4341 2031 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3452 4060 5515 618
Size: 325 217 627 1665 608 1455 1842
Fragments arranged by size:
1842 1665 1455 627 608 325 217
BanII G_rGCy'C
Cuts at: 1391 3005 3723 4058 1391
Size: 1614 718 335 4072
Fragments arranged by size:
4072 1614 718 335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 3062 928
Size: 971 222 941 4605
Fragments arranged by size:
4605 971 941 222
BbvCI CC'TCA_GC
Cuts at: 2892 3724 2892
Size: 832 5907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2655 2689 4930 4964 1903
Size: 34 718 34 2241 34 3678
Fragments arranged by size:
3678 2241 718 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3048 3911 4631 5841 3048
Size: 863 720 1210 3946
Fragments arranged by size:
3946 1210 863 720
BclI T'GATC_A
Cuts at: 1548 2197 3904 1548
Size: 649 1707 4383
Fragments arranged by size:
4383 1707 649
BfrBI ATG'CAT
Cuts at: 2666 2666
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2888 2962 5350
Size: 107 122 71 631 605 1216 74 2388
Cuts at: 5350 136
Size: 1525
Fragments arranged by size:
2388 1525 1216 631 605 122 107 74
71
BglII A'GATC_T
Cuts at: 2573 3046 6734 2573
Size: 473 3688 2578
Fragments arranged by size:
3688 2578 473
BlpI GC'TnA_GC
Cuts at: 4156 4156
Size: 6739
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4800 5956 1790
Size: 1933 1077 1156 2573
Fragments arranged by size:
2573 1933 1156 1077
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2395 3766 4470
Size: 416 53 83 399 24 978 1371 704
Cuts at: 4470 5428 442
Size: 958 1753
Fragments arranged by size:
1753 1371 978 958 704 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 2450 2643 2951 3033 3189 3321 3333
Size: 1008 203 193 308 82 156 132 12
Cuts at: 3333 3396 3597 3791 5399 1239
Size: 63 201 194 1608 2579
Fragments arranged by size:
2579 1608 1008 308 203 201 194 193
156 132 82 63 12
Bpu10I CC'TnA_GC
Cuts at: 2892 3724 2892
Size: 832 5907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4864 5729 6027 4864
Size: 865 298 5576
Fragments arranged by size:
5576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5402 882
Size: 617 3903 2219
Fragments arranged by size:
3903 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3550 4530 493
Size: 711 2346 980 2702
Fragments arranged by size:
2702 2346 980 711
BsaBI GATnn'nnATC
Cuts at: 6733 6733
Size: 6739
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4546 4928 5563 275
Size: 53 83 186 936 3013 382 635 1451
Fragments arranged by size:
3013 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2424 5172 5570 5844 5991 6402 2424
Size: 2748 398 274 147 411 2761
Fragments arranged by size:
2761 2748 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3092 3122 1178
Size: 30 165 30 1689 30 4795
Fragments arranged by size:
4795 1689 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 1186
Size: 1429 5310
BseYI C'CCAG_C
Cuts at: 3595 5942 6438 3595
Size: 2347 496 3896
Fragments arranged by size:
3896 2347 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2195 1163
Size: 573 303 156 5707
Fragments arranged by size:
5707 573 303 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2263 3420 4800 4885 5956 2263
Size: 1157 1380 85 1071 3046
Fragments arranged by size:
3046 1380 1157 1071 85
BsmI GAATG_Cn'
Cuts at: 3069 4250 4343 3069
Size: 1181 93 5465
Fragments arranged by size:
5465 1181 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 3005 3420 3723 4058 4800 4885
Size: 399 473 742 415 303 335 742 85
Cuts at: 4885 5956 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 742 473 415 399 335
303 85
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2044 4628 6358 2044
Size: 2584 1730 2425
Fragments arranged by size:
2584 2425 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3578 3634 4094 844
Size: 2734 56 460 3489
Fragments arranged by size:
3489 2734 460 56
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3514 3876 5234 5408 65
Size: 1183 2266 362 1358 174 1396
Fragments arranged by size:
2266 1396 1358 1183 362 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5383 5570 1700
Size: 110 3573 187 2869
Fragments arranged by size:
3573 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3108 96
Size: 3012 3727
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6739
BstBI TT'CG_AA
Cuts at: 5543 5543
Size: 6739
BstEII G'GTnAC_C
Cuts at: 1184 2563 3610 1184
Size: 1379 1047 4313
Fragments arranged by size:
4313 1379 1047
BstXI CCAn_nnnn'nTGG
Cuts at: 3343 3343
Size: 6739
BstYI r'GATC_y
Cuts at: 1972 2038 2573 3046 4069 4436 4821 4838 6290
Size: 66 535 473 1023 367 385 17 1452
Cuts at: 6290 6376 6388 6734 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1023 535 473 385 367 346
86 66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3387 3582 513
Size: 552 939 1383 195 3670
Fragments arranged by size:
3670 1383 939 552 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 3027 3039 4029 4255 5049 5069 3027
Size: 12 990 226 794 20 4697
Fragments arranged by size:
4697 990 794 226 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 3561 4043 3561
Size: 482 6257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3661 4186 5075
Size: 54 1425 50 275 18 1831 525 889
Cuts at: 5075 6704 8
Size: 1629 43
Fragments arranged by size:
1831 1629 1425 889 525 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 4669 1353
Size: 1903 1413 3423
Fragments arranged by size:
3423 1903 1413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5326 5700 5846 387
Size: 1064 138 3737 374 146 1280
Fragments arranged by size:
3737 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2450 2643 2829 2909 2951 3033
Size: 393 615 203 193 186 80 42 82
Cuts at: 3033 3189 3321 3333 3396 3597 3791 4802 5399
Size: 156 132 12 63 201 194 1011 597
Cuts at: 5399 6186 6454 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1011 787 615 597 393 268 203
201 194 193 186 156 132 82 80
63 42 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2946 2946
Size: 6739
EcoO109I rG'GnC_Cy
Cuts at: 2898 3392 3719 3720 2898
Size: 494 327 1 5917
Fragments arranged by size:
5917 494 327 1
EcoRI G'AATT_C
Cuts at: 2450 4048 4426 2450
Size: 1598 378 4763
Fragments arranged by size:
4763 1598 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3157 3157
Size: 6739
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 2131 2854 3408 5886 1678
Size: 453 723 554 2478 2531
Fragments arranged by size:
2531 2478 723 554 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 2524 2556 2616
Size: 32 135 32 637 32 283 32 60
Cuts at: 2616 2648 3384 3416 3733 3765 3909 3941 5380
Size: 32 736 32 317 32 144 32 1439
Cuts at: 5380 5412 5454 5486 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1439 736 637 317 283 144 135
60 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 2477 4338 2477
Size: 1861 4878
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2428 5853 6037 1305
Size: 1123 3425 184 2007
Fragments arranged by size:
3425 2007 1123 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3663 10
Size: 54 1425 343 1831 3086
Fragments arranged by size:
3086 1831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3032 3912 4697 5056 5215 518
Size: 648 1866 880 785 359 159 2042
Fragments arranged by size:
2042 1866 880 785 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2621 3575 3599 3811 3848 4155 4832 5980
Size: 1015 954 24 212 37 307 677 1148
Cuts at: 5980 6225 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1015 954 677 307 245 212
37 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 3582 513
Size: 552 939 1578 3670
Fragments arranged by size:
3670 1578 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6739
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NsiI A_TGCA'T
Cuts at: 2668 2668
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2489 2584 4097 5642 1132
Size: 97 1260 95 1513 1545 2229
Fragments arranged by size:
2229 1545 1513 1260 97 95
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6739
PfoI T'CCnGG_A
Cuts at: 2466 3420 3708 2466
Size: 954 288 5497
Fragments arranged by size:
5497 954 288
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PmlI CAC'GTG
Cuts at: 3550 3550
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3392 3392
Size: 6739
PshAI GACnn'nnGTC
Cuts at: 1613 2935 1613
Size: 1322 5417
PsiI TTA'TAA
Cuts at: 4309 4309
Size: 6739
PspOMI G'GGCC_C
Cuts at: 3719 3719
Size: 6739
PspXI vC'TCGA_Gb
Cuts at: 3750 3750
Size: 6739
PstI C_TGCA'G
Cuts at: 830 3034 3645 3748 4091 830
Size: 2204 611 103 343 3478
Fragments arranged by size:
3478 2204 611 343 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2621 3575 3599 2621
Size: 954 24 5761
Fragments arranged by size:
5761 954 24
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SanDI GG'GwC_CC
Cuts at: 3392 3392
Size: 6739
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 2080 4987 6699 2080
Size: 2907 1712 2120
Fragments arranged by size:
2907 2120 1712
SexAI A'CCwGG_T
Cuts at: 2640 2640
Size: 6739
SfcI C'TryA_G
Cuts at: 826 1045 2066 3030 3042 3169 3641 3744 4087
Size: 219 1021 964 12 127 472 103 343
Cuts at: 4087 5222 5903 6094 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 964 681 472 343 219
191 127 103 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3750 4843 5744 6006 820
Size: 197 926 1807 1093 901 262 1553
Fragments arranged by size:
1807 1553 1093 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4530 493
Size: 4037 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 2956 3837 1517
Size: 1439 881 4419
Fragments arranged by size:
4419 1439 881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3582 3786 4178 513
Size: 552 939 201 1377 204 392 3074
Fragments arranged by size:
3074 1377 939 552 392 204 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4766 4783 4936 5121 5566 325
Size: 1255 3186 17 153 185 445 1498
Fragments arranged by size:
3186 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2078 3108 4985
Size: 274 80 33 51 1443 101 1030 1877
Cuts at: 4985 6697 96
Size: 1712 138
Fragments arranged by size:
1877 1712 1443 1030 274 138 101 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 3080 2278
Size: 802 5937
XhoI C'TCGA_G
Cuts at: 1943 3750 1943
Size: 1807 4932
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PsrI RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI