(Circular) (Six-base) MAPSORT of: pFN21AB3490.seq Check: 2618 from: 1 to: 5716 pFN21AB3490 5716 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2723 2723 Size: 5716 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 3059 1937 Size: 772 350 4594 Fragments arranged by size: 4594 772 350 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2831 2865 3779 5163 5431 1632 Size: 633 566 34 914 1384 268 1917 Fragments arranged by size: 1917 1384 914 633 566 268 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2566 4615 5592 1203 Size: 1363 2049 977 1327 Fragments arranged by size: 2049 1363 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 2095 2457 3459 5031 2095 Size: 362 1002 1572 2780 Fragments arranged by size: 2780 1572 1002 362 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2249 2435 3025 3251 3403 5512 1493 Size: 756 186 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 756 590 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2837 2870 2837 Size: 33 5683 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2724 2939 3037 4492 618 Size: 325 217 627 937 215 98 1455 1842 Fragments arranged by size: 1842 1455 937 627 325 217 215 98 BanII G_rGCy'C Cuts at: 1391 2653 2966 3035 1391 Size: 1262 313 69 4072 Fragments arranged by size: 4072 1262 313 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 928 Size: 971 261 4484 Fragments arranged by size: 4484 971 261 BbvCI CC'TCA_GC Cuts at: 2014 2014 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4327 136 Size: 107 122 71 631 605 2655 1525 Fragments arranged by size: 2655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 2960 3133 2960 Size: 173 5543 Bme1580I G_kGCm'C Cuts at: 1790 2727 3777 4933 1790 Size: 937 1050 1156 2573 Fragments arranged by size: 2573 1156 1050 937 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 4405 442 Size: 416 53 83 399 24 2030 958 1753 Fragments arranged by size: 2030 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2334 1056 Size: 1278 4438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 Bpu10I CC'TnA_GC Cuts at: 2014 2321 2014 Size: 307 5409 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 2437 2717 3841 4706 5004 2006 Size: 431 280 1124 865 298 2718 Fragments arranged by size: 2718 1124 865 431 298 280 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2988 4379 882 Size: 617 504 985 1391 2219 Fragments arranged by size: 2219 1391 985 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 2403 3523 3905 Size: 53 83 186 936 855 15 1120 382 Cuts at: 3905 4540 275 Size: 635 1451 Fragments arranged by size: 1451 1120 936 855 635 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2714 1186 Size: 1528 4188 BseYI C'CCAG_C Cuts at: 4919 5415 4919 Size: 496 5220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2494 2701 1163 Size: 573 758 207 4178 Fragments arranged by size: 4178 758 573 207 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2672 2966 3777 3862 4933 2672 Size: 294 811 85 1071 3455 Fragments arranged by size: 3455 1071 811 294 85 BsmI GAATG_Cn' Cuts at: 2281 2416 3227 3320 2281 Size: 135 811 93 4677 Fragments arranged by size: 4677 811 135 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2653 2672 2727 2966 3035 3777 3862 Size: 399 863 19 55 239 69 742 85 Cuts at: 3862 4933 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 863 742 399 239 85 69 55 19 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2723 3071 844 Size: 1226 653 348 3489 Fragments arranged by size: 3489 1226 653 348 BsrBI CCG'CTC Cuts at: 1394 1823 2731 3603 1394 Size: 429 908 872 3507 Fragments arranged by size: 3507 908 872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2742 2970 4211 4385 65 Size: 1183 1494 228 1241 174 1396 Fragments arranged by size: 1494 1396 1241 1183 228 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 4360 4547 1700 Size: 110 272 2278 187 2869 Fragments arranged by size: 2869 2278 272 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 2512 3776 4788 2512 Size: 1264 1012 3440 Fragments arranged by size: 3440 1264 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 2887 4520 2887 Size: 1633 4083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2755 2266 Size: 489 5227 BstYI r'GATC_y Cuts at: 1972 2952 3046 3413 3798 3815 5267 5353 5365 Size: 980 94 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 980 385 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2259 513 Size: 552 939 36 219 3970 Fragments arranged by size: 3970 939 552 219 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2822 3232 4026 4046 2822 Size: 410 794 20 4492 Fragments arranged by size: 4492 794 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 3163 4052 Size: 54 1425 50 275 18 254 1079 889 Cuts at: 4052 5681 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1079 889 275 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2416 2574 3646 1353 Size: 1063 158 1072 3423 Fragments arranged by size: 3423 1072 1063 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2964 2964 Size: 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2831 2865 3779 4376 5163 5431 Size: 393 633 566 34 914 597 787 268 Cuts at: 5431 1239 Size: 1524 Fragments arranged by size: 1524 914 787 633 597 566 393 268 34 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 EcoRV GAT'ATC Cuts at: 2833 2833 Size: 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2301 2333 2445 2477 2755 Size: 32 135 32 729 32 112 32 278 Cuts at: 2755 2787 2861 2893 4357 4389 4431 4463 1373 Size: 32 74 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 729 278 135 112 74 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2869 3060 3306 669 Size: 988 281 931 191 246 3079 Fragments arranged by size: 3079 988 931 281 246 191 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2675 3674 4033 4192 518 Size: 648 1509 999 359 159 2042 Fragments arranged by size: 2042 1509 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2508 2959 3132 3809 4957 5202 1606 Size: 436 466 451 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 466 451 436 245 173 NcoI C'CATG_G Cuts at: 513 1065 2004 2259 513 Size: 552 939 255 3970 Fragments arranged by size: 3970 939 552 255 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 2330 1052 Size: 1278 4438 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2570 2644 3074 4619 1132 Size: 97 1341 74 430 1545 2229 Fragments arranged by size: 2229 1545 1341 430 97 74 PasI CC'CwG_GG Cuts at: 1348 2067 2189 1348 Size: 719 122 4875 Fragments arranged by size: 4875 719 122 PciI A'CATG_T Cuts at: 2566 4615 2566 Size: 2049 3667 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2959 2959 Size: 5716 SacI G_AGCT'C Cuts at: 2966 2966 Size: 5716 SacII CC_GC'GG Cuts at: 2043 2043 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 2791 3064 4199 4880 5071 826 Size: 219 1746 273 1135 681 191 1471 Fragments arranged by size: 1746 1471 1135 681 273 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2416 2732 3820 4721 4983 Size: 197 926 78 395 316 1088 901 262 Cuts at: 4983 820 Size: 1553 Fragments arranged by size: 1553 1088 926 901 395 316 262 197 78 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 2617 152 Size: 2465 3251 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 2346 3640 5 Size: 47 2294 1294 2081 Fragments arranged by size: 2294 2081 1294 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2004 2259 3155 513 Size: 552 939 255 896 3074 Fragments arranged by size: 3074 939 896 552 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2172 2304 2329 2516 3743 3760 3913 Size: 1255 592 132 25 187 1227 17 153 Cuts at: 3913 4098 4543 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1227 592 445 187 185 153 132 25 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2922 3962 5674 Size: 274 80 33 51 1443 945 1040 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1712 1443 1040 945 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI