Restriction map for
pFN21AB3490
(Circular) (Six-base) MAPSORT of: pFN21AB3490.seq Check: 2618 from: 1 to: 5716
pFN21AB3490 5716 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2723 2723
Size: 5716
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 3059 1937
Size: 772 350 4594
Fragments arranged by size:
4594 772 350
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2831 2865 3779 5163 5431 1632
Size: 633 566 34 914 1384 268 1917
Fragments arranged by size:
1917 1384 914 633 566 268 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2566 4615 5592 1203
Size: 1363 2049 977 1327
Fragments arranged by size:
2049 1363 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2095 2457 3459 5031 2095
Size: 362 1002 1572 2780
Fragments arranged by size:
2780 1572 1002 362
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2249 2435 3025 3251 3403 5512 1493
Size: 756 186 590 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 590 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2837 2870 2837
Size: 33 5683
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2724 2939 3037 4492 618
Size: 325 217 627 937 215 98 1455 1842
Fragments arranged by size:
1842 1455 937 627 325 217 215 98
BanII G_rGCy'C
Cuts at: 1391 2653 2966 3035 1391
Size: 1262 313 69 4072
Fragments arranged by size:
4072 1262 313 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 928
Size: 971 261 4484
Fragments arranged by size:
4484 971 261
BbvCI CC'TCA_GC
Cuts at: 2014 2014
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 2960 3133 2960
Size: 173 5543
Bme1580I G_kGCm'C
Cuts at: 1790 2727 3777 4933 1790
Size: 937 1050 1156 2573
Fragments arranged by size:
2573 1156 1050 937
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2334 1056
Size: 1278 4438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4376 1239
Size: 3137 2579
Bpu10I CC'TnA_GC
Cuts at: 2014 2321 2014
Size: 307 5409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2437 2717 3841 4706 5004 2006
Size: 431 280 1124 865 298 2718
Fragments arranged by size:
2718 1124 865 431 298 280
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2988 4379 882
Size: 617 504 985 1391 2219
Fragments arranged by size:
2219 1391 985 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 2403 3523 3905
Size: 53 83 186 936 855 15 1120 382
Cuts at: 3905 4540 275
Size: 635 1451
Fragments arranged by size:
1451 1120 936 855 635 382 186 83
53 15
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2714 1186
Size: 1528 4188
BseYI C'CCAG_C
Cuts at: 4919 5415 4919
Size: 496 5220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2494 2701 1163
Size: 573 758 207 4178
Fragments arranged by size:
4178 758 573 207
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2672 2966 3777 3862 4933 2672
Size: 294 811 85 1071 3455
Fragments arranged by size:
3455 1071 811 294 85
BsmI GAATG_Cn'
Cuts at: 2281 2416 3227 3320 2281
Size: 135 811 93 4677
Fragments arranged by size:
4677 811 135 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2653 2672 2727 2966 3035 3777 3862
Size: 399 863 19 55 239 69 742 85
Cuts at: 3862 4933 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 239 85 69
55 19
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2723 3071 844
Size: 1226 653 348 3489
Fragments arranged by size:
3489 1226 653 348
BsrBI CCG'CTC
Cuts at: 1394 1823 2731 3603 1394
Size: 429 908 872 3507
Fragments arranged by size:
3507 908 872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 2970 4211 4385 65
Size: 1183 1494 228 1241 174 1396
Fragments arranged by size:
1494 1396 1241 1183 228 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4360 4547 1700
Size: 110 272 2278 187 2869
Fragments arranged by size:
2869 2278 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 2512 3776 4788 2512
Size: 1264 1012 3440
Fragments arranged by size:
3440 1264 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 2887 4520 2887
Size: 1633 4083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2755 2266
Size: 489 5227
BstYI r'GATC_y
Cuts at: 1972 2952 3046 3413 3798 3815 5267 5353 5365
Size: 980 94 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 980 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2259 513
Size: 552 939 36 219 3970
Fragments arranged by size:
3970 939 552 219 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2822 3232 4026 4046 2822
Size: 410 794 20 4492
Fragments arranged by size:
4492 794 410 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 3163 4052
Size: 54 1425 50 275 18 254 1079 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1079 889 275 254 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2416 2574 3646 1353
Size: 1063 158 1072 3423
Fragments arranged by size:
3423 1072 1063 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2964 2964
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2831 2865 3779 4376 5163 5431
Size: 393 633 566 34 914 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 914 787 633 597 566 393 268
34
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
EcoRV GAT'ATC
Cuts at: 2833 2833
Size: 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2301 2333 2445 2477 2755
Size: 32 135 32 729 32 112 32 278
Cuts at: 2755 2787 2861 2893 4357 4389 4431 4463 1373
Size: 32 74 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 729 278 135 112 74 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3060 3306 669
Size: 988 281 931 191 246 3079
Fragments arranged by size:
3079 988 931 281 246 191
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 3674 4033 4192 518
Size: 648 1509 999 359 159 2042
Fragments arranged by size:
2042 1509 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2508 2959 3132 3809 4957 5202 1606
Size: 436 466 451 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 466 451 436 245 173
NcoI C'CATG_G
Cuts at: 513 1065 2004 2259 513
Size: 552 939 255 3970
Fragments arranged by size:
3970 939 552 255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 2330 1052
Size: 1278 4438
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2570 2644 3074 4619 1132
Size: 97 1341 74 430 1545 2229
Fragments arranged by size:
2229 1545 1341 430 97 74
PasI CC'CwG_GG
Cuts at: 1348 2067 2189 1348
Size: 719 122 4875
Fragments arranged by size:
4875 719 122
PciI A'CATG_T
Cuts at: 2566 4615 2566
Size: 2049 3667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2959 2959
Size: 5716
SacI G_AGCT'C
Cuts at: 2966 2966
Size: 5716
SacII CC_GC'GG
Cuts at: 2043 2043
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 2791 3064 4199 4880 5071 826
Size: 219 1746 273 1135 681 191 1471
Fragments arranged by size:
1746 1471 1135 681 273 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2416 2732 3820 4721 4983
Size: 197 926 78 395 316 1088 901 262
Cuts at: 4983 820
Size: 1553
Fragments arranged by size:
1553 1088 926 901 395 316 262 197
78
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 2617 152
Size: 2465 3251
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 2346 3640 5
Size: 47 2294 1294 2081
Fragments arranged by size:
2294 2081 1294 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2004 2259 3155 513
Size: 552 939 255 896 3074
Fragments arranged by size:
3074 939 896 552 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2172 2304 2329 2516 3743 3760 3913
Size: 1255 592 132 25 187 1227 17 153
Cuts at: 3913 4098 4543 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1227 592 445 187 185 153
132 25 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 3962 5674
Size: 274 80 33 51 1443 945 1040 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1040 945 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI