Restriction map for
pFN21AB3488
(Circular) (Six-base) MAPSORT of: pFN21AB3488.seq Check: 5908 from: 1 to: 5755
pFN21AB3488 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 2616 3882 4255 1563
Size: 1053 1266 373 3063
Fragments arranged by size:
3063 1266 1053 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 3818 5202 5470 1632
Size: 1412 774 1384 268 1917
Fragments arranged by size:
1917 1412 1384 774 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2214 1166
Size: 1048 4707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2554 2586 2554
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 2930 3498 5070 2930
Size: 568 1572 3615
Fragments arranged by size:
3615 1572 568
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2347 3064 3290 3442 5551 1493
Size: 854 717 226 152 2109 1697
Fragments arranged by size:
2109 1697 854 717 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2484 4309 160
Size: 872 1452 1825 1606
Fragments arranged by size:
1825 1606 1452 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
AvrII C'CTAG_G
Cuts at: 2578 2578
Size: 5755
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BbvCI CC'TCA_GC
Cuts at: 2999 2999
Size: 5755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2222 2256 3946 3980 1903
Size: 34 285 34 1690 34 3678
Fragments arranged by size:
3678 1690 285 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2781 2796 1548
Size: 1233 15 4507
Fragments arranged by size:
4507 1233 15
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 2907 2952 4415 1239
Size: 1197 471 45 1463 2579
Fragments arranged by size:
2579 1463 1197 471 45
Bpu10I CC'TnA_GC
Cuts at: 2999 2999
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2799 2829 1178
Size: 30 165 30 648 30 718 30 4104
Fragments arranged by size:
4104 718 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5755
BseYI C'CCAG_C
Cuts at: 2142 2909 4958 5454 2142
Size: 767 2049 496 2443
Fragments arranged by size:
2443 2049 767 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 1163
Size: 573 891 4291
Fragments arranged by size:
4291 891 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 2568 2628 3266 3359 2568
Size: 60 638 93 4964
Fragments arranged by size:
4964 638 93 60
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3074 3816 3901 4972 1391
Size: 399 1284 742 85 1071 2174
Fragments arranged by size:
2174 1284 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 3110 844
Size: 1912 354 3489
Fragments arranged by size:
3489 1912 354
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2446 2628 2640 2964 4250 4424 65
Size: 1183 1198 182 12 324 1286 174 1396
Fragments arranged by size:
1396 1286 1198 1183 324 182 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2524 4399 4586 1700
Size: 110 714 1875 187 2869
Fragments arranged by size:
2869 1875 714 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 794
Size: 1367 4388
BstBI TT'CG_AA
Cuts at: 2104 4559 2104
Size: 2455 3300
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2953 2953
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2433 3085 3452 3837 3854 5306 5392 5404
Size: 461 652 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 652 461 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2191 2191
Size: 5755
BtgI C'CryG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 3734
Fragments arranged by size:
3734 939 552 530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 2701 1554
Size: 1147 4608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2204 3202 4091
Size: 54 1425 50 275 18 374 998 889
Cuts at: 4091 5720 8
Size: 1629 43
Fragments arranged by size:
1629 1425 998 889 374 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2412 3003 3685 1353
Size: 819 240 591 682 3423
Fragments arranged by size:
3423 819 682 591 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 2907 2952 3044 3818 4415 5202
Size: 393 804 471 45 92 774 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 774 597 471 393 268
92 45
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2799 2831 4396 4428 4470
Size: 32 135 32 1227 32 1565 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1565 1227 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2597 2599 2854 4869 5053 1305
Size: 1292 2 255 2015 184 2007
Fragments arranged by size:
2015 2007 1292 255 184 2
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 2253 2421 2633 2649 2822 2951
Size: 648 1048 39 168 212 16 173 129
Cuts at: 2951 3713 4072 4231 518
Size: 762 359 159 2042
Fragments arranged by size:
2042 1048 762 648 359 212 173 168
159 129 39 16
MspA1I CmG'CkG
Cuts at: 1606 2064 2279 3171 3848 4996 5241 1606
Size: 458 215 892 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 677 458 245 215
NcoI C'CATG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 3734
Fragments arranged by size:
3734 939 552 530
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2930 1108
Size: 1822 3933
PfoI T'CCnGG_A
Cuts at: 2668 2929 2668
Size: 261 5494
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2052 2284 2767 3107 830
Size: 1222 232 483 340 3478
Fragments arranged by size:
3478 1222 483 340 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2064 2279 2064
Size: 215 5540
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SapI GCTCTTCn'nnn_
Cuts at: 3003 3003
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2048 2280 2763 3103 4238 4919 5110
Size: 219 1003 232 483 340 1135 681 191
Cuts at: 5110 826
Size: 1471
Fragments arranged by size:
1471 1135 1003 681 483 340 232 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2534 2578 3194 513
Size: 552 939 530 44 616 3074
Fragments arranged by size:
3074 939 616 552 530 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 3782 3799 3952 4137 4582 325
Size: 1255 593 1609 17 153 185 445 1498
Fragments arranged by size:
1609 1498 1255 593 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 2911 2943 5715 5747 2051
Size: 32 828 32 2772 32 2059
Fragments arranged by size:
2772 2059 828 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2541 2866 2541
Size: 325 5430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI