Restriction map for
pFN21AB3479
(Circular) (Six-base) MAPSORT of: pFN21AB3479.seq Check: 4012 from: 1 to: 5968
pFN21AB3479 5968 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2822 2822
Size: 5968
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2219 2942 3311 1937
Size: 282 723 369 4594
Fragments arranged by size:
4594 723 369 282
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4031 5415 5683 1632
Size: 2399 1384 268 1917
Fragments arranged by size:
2399 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2484 1184
Size: 1300 4668
AlwNI CAG_nnn'CTG
Cuts at: 2300 2814 3711 5283 2300
Size: 514 897 1572 2985
Fragments arranged by size:
2985 1572 897 514
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2253 3277 3503 3655 5764 1493
Size: 760 1024 226 152 2109 1697
Fragments arranged by size:
2109 1697 1024 760 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 3293 1870
Size: 73 1350 4545
Fragments arranged by size:
4545 1350 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2787 2967 3289 4744 618
Size: 325 217 627 1000 180 322 1455 1842
Fragments arranged by size:
1842 1455 1000 627 325 322 217 180
BanII G_rGCy'C
Cuts at: 1391 2065 2347 3287 1391
Size: 674 282 940 4072
Fragments arranged by size:
4072 940 674 282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 928
Size: 971 315 4682
Fragments arranged by size:
4682 971 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BfrBI ATG'CAT
Cuts at: 3155 3155
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2765 4579 136
Size: 107 122 71 631 605 1093 1814 1525
Fragments arranged by size:
1814 1525 1093 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 2685 3385 2685
Size: 700 5268
Bme1580I G_kGCm'C
Cuts at: 1790 2434 4029 5185 1790
Size: 644 1595 1156 2573
Fragments arranged by size:
2573 1595 1156 644
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3699 4657 442
Size: 416 53 83 399 24 2282 958 1753
Fragments arranged by size:
2282 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2577 2577
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2652 4628 1239
Size: 895 518 1976 2579
Fragments arranged by size:
2579 1976 895 518
Bpu10I CC'TnA_GC
Cuts at: 2038 2038
Size: 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 4093 4958 5256 2188
Size: 1905 865 298 2900
Fragments arranged by size:
2900 1905 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4631 882
Size: 617 3132 2219
Fragments arranged by size:
3132 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3025 3759 493
Size: 711 1821 734 2702
Fragments arranged by size:
2702 1821 734 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4157 4792 275
Size: 53 83 186 936 2242 382 635 1451
Fragments arranged by size:
2242 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2439 4401 4799 5073 5220 5631 2439
Size: 1962 398 274 147 411 2776
Fragments arranged by size:
2776 1962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2337 1186
Size: 1151 4817
BseYI C'CCAG_C
Cuts at: 2650 2746 2791 3011 5171 5667 2650
Size: 96 45 220 2160 496 2951
Fragments arranged by size:
2951 2160 496 220 96 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2454 3140 1163
Size: 573 354 364 686 3991
Fragments arranged by size:
3991 686 573 364 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3179 4029 4114 5185 2691
Size: 488 850 85 1071 3474
Fragments arranged by size:
3474 1071 850 488 85
BsmI GAATG_Cn'
Cuts at: 3003 3479 3572 3003
Size: 476 93 5399
Fragments arranged by size:
5399 476 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2347 2434 2691 3179 3287 4029
Size: 399 275 282 87 257 488 108 742
Cuts at: 4029 4114 5185 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 399 282 275 257
108 87 85
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 3323 844
Size: 1978 501 3489
Fragments arranged by size:
3489 1978 501
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2179 2278 2941 4463 4637 65
Size: 1183 931 99 663 1522 174 1396
Fragments arranged by size:
1522 1396 1183 931 663 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 4612 4799 1700
Size: 110 2802 187 2869
Fragments arranged by size:
2869 2802 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 2045 4028 5040 2045
Size: 1983 1012 2973
Fragments arranged by size:
2973 1983 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2383 2814 3057 3192 794
Size: 1589 431 243 135 3570
Fragments arranged by size:
3570 1589 431 243 135
BstBI TT'CG_AA
Cuts at: 2100 4772 2100
Size: 2672 3296
BstEII G'GTnAC_C
Cuts at: 1184 2741 1184
Size: 1557 4411
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963
Size: 1326 367 385 17 1452 86 12 346
Cuts at: 5963 1972
Size: 1977
Fragments arranged by size:
1977 1452 1326 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2220 2943 2220
Size: 723 5245
BtgI C'CryG_G
Cuts at: 513 1065 2004 2667 513
Size: 552 939 663 3814
Fragments arranged by size:
3814 939 663 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3484 4278 4298 2664
Size: 820 794 20 4334
Fragments arranged by size:
4334 820 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2212 2247 529
Size: 35 1648 35 4250
Fragments arranged by size:
4250 1648 35 35
DraI TTT'AAA
Cuts at: 2073 3272 2073
Size: 1199 4769
DraIII CAC_nnn'GTG
Cuts at: 3079 3165 3079
Size: 86 5882
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2370 3415
Size: 54 1425 50 275 18 313 227 1045
Cuts at: 3415 4304 5933 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1045 889 313 275 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2172 3251 3898 1353
Size: 792 27 1079 647 3423
Fragments arranged by size:
3423 1079 792 647 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2652 4031 4628 5415 5683 1239
Size: 393 502 518 1379 597 787 268 1524
Fragments arranged by size:
1524 1379 787 597 518 502 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2482 2609 2482
Size: 127 5841
EcoRI G'AATT_C
Cuts at: 2253 3277 3655 2253
Size: 1024 378 4566
Fragments arranged by size:
4566 1024 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 5968
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 5115 1678
Size: 3437 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 4609 4641 4683
Size: 32 135 32 1091 32 1914 32 42
Cuts at: 4683 4715 1373
Size: 32 2626
Fragments arranged by size:
2626 1914 1091 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 3312 3558 669
Size: 988 281 895 479 246 3079
Fragments arranged by size:
3079 988 895 479 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5082 5266 1305
Size: 3777 184 2007
Fragments arranged by size:
3777 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 2372 10
Size: 54 1425 343 313 227 3606
Fragments arranged by size:
3606 1425 343 313 227 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2883 3926 4285 4444 518
Size: 648 1221 496 1043 359 159 2042
Fragments arranged by size:
2042 1221 1043 648 496 359 159
MspA1I CmG'CkG
Cuts at: 1606 2452 3147 3384 4061 5209 5454 1606
Size: 846 695 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 695 677 245 237
NcoI C'CATG_G
Cuts at: 513 1065 2004 2667 513
Size: 552 939 663 3814
Fragments arranged by size:
3814 939 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NsiI A_TGCA'T
Cuts at: 3157 3157
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2386 3155 3326 4871 1132
Size: 97 1157 769 171 1545 2229
Fragments arranged by size:
2229 1545 1157 769 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5968
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5968
PfoI T'CCnGG_A
Cuts at: 2629 2629
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 2482
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2810 3188 3320 830
Size: 1980 378 132 3478
Fragments arranged by size:
3478 1980 378 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2452 3147 2452
Size: 695 5273
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2145 2145
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2806 3184 3316 4451 5132 5323 826
Size: 219 1761 378 132 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 378 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 4072 4973 5235 820
Size: 197 926 224 1905 901 262 1553
Fragments arranged by size:
1905 1553 926 901 262 224 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3155 3326 1132
Size: 2023 171 3774
Fragments arranged by size:
3774 2023 171
SspI AAT'ATT
Cuts at: 5 52 2705 3892 5
Size: 47 2653 1187 2081
Fragments arranged by size:
2653 2081 1187 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 2605 2667 3407 513
Size: 552 939 393 208 62 740 3074
Fragments arranged by size:
3074 939 740 552 393 208 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2805 3995 4012 4165 4350 4795 325
Size: 1255 1225 1190 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1225 1190 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4214 5926
Size: 274 80 33 51 1443 402 1835 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1835 1712 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 4097 2978
Size: 1119 4849
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI