Restriction map for
pFN21AB3475
(Circular) (Six-base) MAPSORT of: pFN21AB3475.seq Check: 3898 from: 1 to: 6181
pFN21AB3475 6181 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3172 4244 5628 5896 1632
Size: 1540 1072 1384 268 1917
Fragments arranged by size:
1917 1540 1384 1072 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 2969 1166
Size: 1803 4378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2630 1184
Size: 1446 4735
AlwNI CAG_nnn'CTG
Cuts at: 2854 3924 5496 2854
Size: 1070 1572 3539
Fragments arranged by size:
3539 1572 1070
ApaI G_GGCC'C
Cuts at: 2470 2470
Size: 6181
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2284 3247 3490 3716 3868 5977 1493
Size: 791 963 243 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 791 243 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 3440 3506 1870
Size: 73 684 813 66 4545
Fragments arranged by size:
4545 813 684 73 66
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 3502 4957 618
Size: 325 217 627 687 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 687 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2470 3500 1391
Size: 622 457 1030 4072
Fragments arranged by size:
4072 1030 622 457
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2866 3295 928
Size: 971 967 429 3814
Fragments arranged by size:
3814 971 967 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2482 2494 2516 2528 4372 4406 1903
Size: 34 545 12 22 12 1844 34 3678
Fragments arranged by size:
3678 1844 545 34 34 22 12 12
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3030 4792 136
Size: 107 122 71 631 605 1358 1762 1525
Fragments arranged by size:
1762 1525 1358 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 2573 3598 2573
Size: 1025 5156
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2470 2477 4242 5398 1790
Size: 637 43 7 1765 1156 2573
Fragments arranged by size:
2573 1765 1156 637 43 7
BmgBI CAC'GTC
Cuts at: 1318 2713 3203 1318
Size: 1395 490 4296
Fragments arranged by size:
4296 1395 490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 3912 4870
Size: 416 53 83 399 24 940 1555 958
Cuts at: 4870 442
Size: 1753
Fragments arranged by size:
1753 1555 958 940 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3071 1056
Size: 2015 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2804 3098 4841 1239
Size: 876 689 294 1743 2579
Fragments arranged by size:
2579 1743 876 689 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3972 493
Size: 711 2768 2702
Fragments arranged by size:
2768 2702 711
BsaBI GATnn'nnATC
Cuts at: 2369 6175 2369
Size: 3806 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2942 4614 5012 5286 5433 5844 2942
Size: 1672 398 274 147 411 3279
Fragments arranged by size:
3279 1672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2046 2076 3272 3302 1178
Size: 30 165 30 643 30 1196 30 4057
Fragments arranged by size:
4057 1196 643 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2070 2141 2678 3458 1186
Size: 884 71 537 780 3909
Fragments arranged by size:
3909 884 780 537 71
BseYI C'CCAG_C
Cuts at: 2012 2488 2847 5384 5880 2012
Size: 476 359 2537 496 2313
Fragments arranged by size:
2537 2313 496 476 359
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2933 1163
Size: 573 729 468 4411
Fragments arranged by size:
4411 729 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 3324 4242 4327 5398 2331
Size: 993 918 85 1071 3114
Fragments arranged by size:
3114 1071 993 918 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2331 2427 2470 2477 3324 3500
Size: 399 223 318 96 43 7 847 176
Cuts at: 3500 4242 4327 5398 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 399 318 223 176
96 85 43 7
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 3054 4070 5800 3054
Size: 1016 1730 3435
Fragments arranged by size:
3435 1730 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2313 2733 3536 844
Size: 1469 420 803 3489
Fragments arranged by size:
3489 1469 803 420
BsrBI CCG'CTC
Cuts at: 1394 1823 2796 4068 1394
Size: 429 973 1272 3507
Fragments arranged by size:
3507 1272 973 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3213 4676 4850 65
Size: 1183 1965 1463 174 1396
Fragments arranged by size:
1965 1463 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2277 3000 4825 5012 1700
Size: 110 467 723 1825 187 2869
Fragments arranged by size:
2869 1825 723 467 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 2887 4241 5253 2887
Size: 1354 1012 3815
Fragments arranged by size:
3815 1354 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2758 794
Size: 1964 4217
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 4592
BstXI CCAn_nnnn'nTGG
Cuts at: 2159 2434 2159
Size: 275 5906
BstYI r'GATC_y
Cuts at: 1972 3511 3878 4263 4280 5732 5818 5830 6176
Size: 1539 367 385 17 1452 86 12 346
Cuts at: 6176 1972
Size: 1977
Fragments arranged by size:
1977 1539 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 3164 513
Size: 552 939 45 1115 3530
Fragments arranged by size:
3530 1115 939 552 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2497 2809 3046 487
Size: 683 56 1271 312 237 3622
Fragments arranged by size:
3622 1271 683 312 237 56
BtsI GCAGTG_nn'
Cuts at: 2067 2609 3071 3697 4491 4511 2067
Size: 542 462 626 794 20 3737
Fragments arranged by size:
3737 794 626 542 462 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2211 2275
Size: 54 1425 50 275 18 216 165 64
Cuts at: 2275 3114 3628 4517 6146 8
Size: 839 514 889 1629 43
Fragments arranged by size:
1629 1425 889 839 514 275 216 165
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3191 4111 1353
Size: 1383 455 920 3423
Fragments arranged by size:
3423 1383 920 455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2296 2311 3019 4768 5142 5288
Size: 1064 138 707 15 708 1749 374 146
Cuts at: 5288 387
Size: 1280
Fragments arranged by size:
1749 1280 1064 708 707 374 146 138
15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2804 3098 3172 4244 4841 5628
Size: 393 483 689 294 74 1072 597 787
Cuts at: 5628 5896 1239
Size: 268 1524
Fragments arranged by size:
1524 1072 787 689 597 483 393 294
268 74
EcoO109I rG'GnC_Cy
Cuts at: 2466 2467 2554 3149 2466
Size: 1 87 595 5498
Fragments arranged by size:
5498 595 87 1
EcoRI G'AATT_C
Cuts at: 2284 3490 3868 2284
Size: 1206 378 4597
Fragments arranged by size:
4597 1206 378
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 5328 1678
Size: 3650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2998 3030 3085 3117 4822
Size: 32 135 32 1426 32 55 32 1705
Cuts at: 4822 4854 4896 4928 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1705 1426 135 55 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 2953 3525 3771 669
Size: 988 281 328 687 572 246 3079
Fragments arranged by size:
3079 988 687 572 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 2266 3771 2266
Size: 1505 4676
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3258 5295 5479 1305
Size: 1953 2037 184 2007
Fragments arranged by size:
2037 2007 1953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 3116 10
Size: 54 1425 343 381 903 3075
Fragments arranged by size:
3075 1425 903 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2393 2432 2828 2969 3053 4139
Size: 648 991 236 39 396 141 84 1086
Cuts at: 4139 4498 4657 518
Size: 359 159 2042
Fragments arranged by size:
2042 1086 991 648 396 359 236 159
141 84 39
MspA1I CmG'CkG
Cuts at: 1606 2488 2708 2842 2854 3209 3597 4274 5422
Size: 882 220 134 12 355 388 677 1148
Cuts at: 5422 5667 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 882 677 388 355 245 220
134 12
NaeI GCC'GGC
Cuts at: 2279 3002 2279
Size: 723 5458
NcoI C'CATG_G
Cuts at: 513 1065 2004 3164 513
Size: 552 939 1160 3530
Fragments arranged by size:
3530 1160 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NgoMIV G'CCGG_C
Cuts at: 2277 3000 2277
Size: 723 5458
NheI G'CTAG_C
Cuts at: 1052 3067 1052
Size: 2015 4166
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3265 3539 5084 1132
Size: 97 2036 274 1545 2229
Fragments arranged by size:
2229 2036 1545 274 97
PasI CC'CwG_GG
Cuts at: 1348 2470 3460 1348
Size: 1122 990 4069
Fragments arranged by size:
4069 1122 990
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2196 3165 3277 3460 1108
Size: 1088 969 112 183 3829
Fragments arranged by size:
3829 1088 969 183 112
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4250 4282 5763 5795 3434
Size: 32 784 32 1481 32 3820
Fragments arranged by size:
3820 1481 784 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 3149 2554
Size: 595 5586
PshAI GACnn'nnGTC
Cuts at: 1613 2147 1613
Size: 534 5647
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2466 2466
Size: 6181
PstI C_TGCA'G
Cuts at: 830 2079 2604 2730 2841 3533 830
Size: 1249 525 126 111 692 3478
Fragments arranged by size:
3478 1249 692 525 126 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2488 2708 2842 2854 3209 2488
Size: 220 134 12 355 5460
Fragments arranged by size:
5460 355 220 134 12
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 2730 3533 2730
Size: 803 5378
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 2075 2600 2726 2837 3529 4664 5345
Size: 219 1030 525 126 111 692 1135 681
Cuts at: 5345 5536 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1030 692 681 525 219 191
126 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4285 5186 5448 820
Size: 197 926 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 3024 1517
Size: 1507 4674
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 3620 513
Size: 552 939 1160 456 3074
Fragments arranged by size:
3074 1160 939 552 456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 2464 4208 4225 4378 4563 5008
Size: 1255 846 38 1744 17 153 185 445
Cuts at: 5008 325
Size: 1498
Fragments arranged by size:
1744 1498 1255 846 445 185 153 38
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 4427 6139
Size: 274 80 33 51 1443 420 2030 1712
Cuts at: 6139 96
Size: 138
Fragments arranged by size:
2030 1712 1443 420 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 3272 3304 6141 6173 2046
Size: 32 1194 32 2837 32 2054
Fragments arranged by size:
2837 2054 1194 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2855 2855
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI