Restriction map for
pFN21AB3471
(Circular) (Six-base) MAPSORT of: pFN21AB3471.seq Check: 7376 from: 1 to: 6307
pFN21AB3471 6307 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3650 1937
Size: 823 890 4594
Fragments arranged by size:
4594 890 823
Acc65I G'GTAC_C
Cuts at: 3628 3628
Size: 6307
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 3091 3146 4370 5754 6022 1632
Size: 1013 446 55 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 1013 446 268 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 2382 5206 6183 1203
Size: 1179 2824 977 1327
Fragments arranged by size:
2824 1327 1179 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 2851 5036 2851
Size: 2185 4122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 4912
AlwNI CAG_nnn'CTG
Cuts at: 2101 2689 2857 3124 4050 5622 2101
Size: 588 168 267 926 1572 2786
Fragments arranged by size:
2786 1572 926 588 267 168
ApaI G_GGCC'C
Cuts at: 2274 2710 2274
Size: 436 5871
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 3616 3842 3994 6103 1493
Size: 2123 226 152 2109 1697
Fragments arranged by size:
2123 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 2254 2273 2468 2744 3064 3632 1870
Size: 73 311 19 195 276 320 568 4545
Fragments arranged by size:
4545 568 320 311 276 195 73 19
BamHI G'GATC_C
Cuts at: 2472 3637 4004 2472
Size: 1165 367 4775
Fragments arranged by size:
4775 1165 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 2361 3239 3251 3395
Size: 325 217 627 542 32 878 12 144
Cuts at: 3395 3580 3628 5083 618
Size: 185 48 1455 1842
Fragments arranged by size:
1842 1455 878 627 542 325 217 185
144 48 32 12
BanII G_rGCy'C
Cuts at: 1391 2055 2274 2280 2710 3626 1391
Size: 664 219 6 430 916 4072
Fragments arranged by size:
4072 916 664 430 219 6
BbeI G_GCGC'C
Cuts at: 2365 3243 3399 2365
Size: 878 156 5273
Fragments arranged by size:
5273 878 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2326 2360 4498 4532 1903
Size: 34 389 34 2138 34 3678
Fragments arranged by size:
3678 2138 389 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 2613 2895 1548
Size: 1065 282 4960
Fragments arranged by size:
4960 1065 282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3271 4918 136
Size: 107 122 71 631 605 1599 1647 1525
Fragments arranged by size:
1647 1599 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3015 3045 3354 3453 6302 3015
Size: 30 309 99 2849 3020
Fragments arranged by size:
3020 2849 309 99 30
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2422 2710 2908 3276 4368 5524 1790
Size: 484 148 288 198 368 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 484 368 288 198 148
BmgBI CAC'GTC
Cuts at: 1318 3313 3472 1318
Size: 1995 159 4153
Fragments arranged by size:
4153 1995 159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2174 2502 2549 3560 4967 1239
Size: 893 42 328 47 1011 1407 2579
Fragments arranged by size:
2579 1407 1011 893 328 47 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2930 4432 5297 5595 2930
Size: 1502 865 298 3642
Fragments arranged by size:
3642 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4970 882
Size: 617 3471 2219
Fragments arranged by size:
3471 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 2264 6301 2264
Size: 4037 2270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2362 2574 3240 3396
Size: 53 83 186 936 829 212 666 156
Cuts at: 3396 4114 4496 5131 275
Size: 718 382 635 1451
Fragments arranged by size:
1451 936 829 718 666 635 382 212
186 156 83 53
BsaWI w'CCGG_w
Cuts at: 4740 5138 5412 5559 5970 4740
Size: 398 274 147 411 5077
Fragments arranged by size:
5077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 3032 3062 1178
Size: 30 165 30 1350 30 249 30 4423
Fragments arranged by size:
4423 1350 249 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2180 2780 2840 3218 3515 1186
Size: 943 51 600 60 378 297 3978
Fragments arranged by size:
3978 943 600 378 297 60 51
BseYI C'CCAG_C
Cuts at: 2013 2421 5510 6006 2013
Size: 408 3089 496 2314
Fragments arranged by size:
3089 2314 496 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5734
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 2121 2190 3042 4368 4453 5524 2055
Size: 66 69 852 1326 85 1071 2838
Fragments arranged by size:
2838 1326 1071 852 85 69 66
BsmI GAATG_Cn'
Cuts at: 3818 3911 3818
Size: 93 6214
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 6307
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2121 2190 2274 2280 2422 2710
Size: 399 265 66 69 84 6 142 288
Cuts at: 2710 2908 3042 3276 3626 4368 4453 5524 1391
Size: 198 134 234 350 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 288 265 234
198 142 134 85 84 69 66 6
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3573 3662 844
Size: 2729 89 3489
Fragments arranged by size:
3489 2729 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 4194 1394
Size: 429 965 1406 3507
Fragments arranged by size:
3507 1406 965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4802 4976 65
Size: 1183 3554 174 1396
Fragments arranged by size:
3554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3293 4951 5138 1700
Size: 110 1483 1658 187 2869
Fragments arranged by size:
2869 1658 1483 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 2132 2192 2345 3548 4367 5379 2132
Size: 60 153 1203 819 1012 3060
Fragments arranged by size:
3060 1203 1012 819 153 60
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 2692 3580 794
Size: 1307 591 888 3521
Fragments arranged by size:
3521 1307 888 591
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstXI CCAn_nnnn'nTGG
Cuts at: 3139 3139
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 2472 2643 3015 3045 3354 3453 3637 4004
Size: 500 171 372 30 309 99 184 367
Cuts at: 4004 4389 4406 5858 5944 5956 6302 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 500 385 372 367 346 309
184 171 99 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 6307
BtgI C'CryG_G
Cuts at: 513 1065 2357 513
Size: 552 1292 4463
Fragments arranged by size:
4463 1292 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2281 3157 487
Size: 683 56 1055 876 3637
Fragments arranged by size:
3637 1055 876 683 56
BtsI GCAGTG_nn'
Cuts at: 2855 3823 4617 4637 2855
Size: 968 794 20 4525
Fragments arranged by size:
4525 968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DraIII CAC_nnn'GTG
Cuts at: 3580 3580
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2913 3162
Size: 54 1425 50 275 18 237 846 249
Cuts at: 3162 3465 3513 3754 4643 6272 8
Size: 303 48 241 889 1629 43
Fragments arranged by size:
1629 1425 889 846 303 275 249 241
237 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2573 2843 4237 1353
Size: 707 513 270 1394 3423
Fragments arranged by size:
3423 1394 707 513 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 6307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2174 2502 2549 2645 3091 3146
Size: 393 500 42 328 47 96 446 55
Cuts at: 3146 3560 4370 4967 5754 6022 1239
Size: 414 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 500 446 414 393
328 268 96 55 47 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 3527 2840
Size: 687 5620
EcoO109I rG'GnC_Cy
Cuts at: 2706 2707 2833 2706
Size: 1 126 6180
Fragments arranged by size:
6180 126 1
EcoRI G'AATT_C
Cuts at: 3616 3994 3616
Size: 378 5929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 6307
FspI TGC'GCA
Cuts at: 2264 4813 2264
Size: 2549 3758
FspAI rTGC'GCAy
Cuts at: 2264 2264
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 2365 3125 3243 3399 5454 1678
Size: 687 760 118 156 2055 2531
Fragments arranged by size:
2531 2055 760 687 156 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2223 2255 2588 2605 2620
Size: 32 135 32 651 32 333 17 15
Cuts at: 2620 2637 2837 2869 4948 4980 5022 5054 1373
Size: 17 200 32 2079 32 42 32 2626
Fragments arranged by size:
2626 2079 651 333 200 135 42 32
32 32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3379 3651 3897 669
Size: 988 281 1441 272 246 3079
Fragments arranged by size:
3079 1441 988 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KasI G'GCGC_C
Cuts at: 2361 3239 3395 2361
Size: 878 156 5273
Fragments arranged by size:
5273 878 156
KpnI G_GTAC'C
Cuts at: 3632 3632
Size: 6307
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 2382 6183 2382
Size: 3801 2506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 5421 5605 1305
Size: 949 3167 184 2007
Fragments arranged by size:
3167 2007 949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2915 3164 3515 10
Size: 54 1425 343 1083 249 351 2802
Fragments arranged by size:
2802 1425 1083 351 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4265 4624 4783 518
Size: 648 3099 359 159 2042
Fragments arranged by size:
3099 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2086 2359 2425 2494 2689 3723 4400
Size: 407 73 273 66 69 195 1034 677
Cuts at: 4400 5548 5793 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1034 677 407 273 245 195
73 69 66
NaeI GCC'GGC
Cuts at: 3295 3295
Size: 6307
NarI GG'CG_CC
Cuts at: 2362 3240 3396 2362
Size: 878 156 5273
Fragments arranged by size:
5273 878 156
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5755
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NgoMIV G'CCGG_C
Cuts at: 3293 3293
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 3160 3665 5210 1132
Size: 97 1931 505 1545 2229
Fragments arranged by size:
2229 1931 1545 505 97
PasI CC'CwG_GG
Cuts at: 1348 2722 3053 1348
Size: 1374 331 4602
Fragments arranged by size:
4602 1374 331
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 2584 1108
Size: 957 519 4831
Fragments arranged by size:
4831 957 519
PfoI T'CCnGG_A
Cuts at: 2439 2526 2439
Size: 87 6220
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 2833
Size: 6307
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PspOMI G'GGCC_C
Cuts at: 2270 2706 2270
Size: 436 5871
PstI C_TGCA'G
Cuts at: 830 3096 3659 830
Size: 2266 563 3478
Fragments arranged by size:
3478 2266 563
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2013 2086 2425 2689 2013
Size: 73 339 264 5631
Fragments arranged by size:
5631 339 264 73
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 6307
SacII CC_GC'GG
Cuts at: 2360 2360
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2573 2060
Size: 513 5794
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SexAI A'CCwGG_T
Cuts at: 3576 3576
Size: 6307
SfcI C'TryA_G
Cuts at: 826 1045 3092 3655 4790 5471 5662 826
Size: 219 2047 563 1135 681 191 1471
Fragments arranged by size:
2047 1471 1135 681 563 219 191
SfoI GGC'GCC
Cuts at: 2363 3241 3397 2363
Size: 878 156 5273
Fragments arranged by size:
5273 878 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 2256 2470 3634 1872
Size: 384 214 1164 4545
Fragments arranged by size:
4545 1164 384 214
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2945 4411 5312 5574 820
Size: 197 926 1002 1466 901 262 1553
Fragments arranged by size:
1553 1466 1002 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2916 3746 513
Size: 552 1851 830 3074
Fragments arranged by size:
3074 1851 830 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3310 4334 4351 4504 4689 5134 325
Size: 1255 1730 1024 17 153 185 445 1498
Fragments arranged by size:
1730 1498 1255 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 4553 6265
Size: 274 80 33 51 1443 225 2351 1712
Cuts at: 6265 96
Size: 138
Fragments arranged by size:
2351 1712 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 6267 6299 3032
Size: 32 3203 32 3040
Fragments arranged by size:
3203 3040 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6307
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2437 3050 3412 2323
Size: 114 613 362 5218
Fragments arranged by size:
5218 613 362 114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 2254 2468 3632 1870
Size: 384 214 1164 4545
Fragments arranged by size:
4545 1164 384 214
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoRV FseI NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI