Restriction map for
pFN21AB3465
(Circular) (Six-base) MAPSORT of: pFN21AB3465.seq Check: 5387 from: 1 to: 5740
pFN21AB3465 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2661 3803 5187 5455 1632
Size: 638 391 1142 1384 268 1917
Fragments arranged by size:
1917 1384 1142 638 391 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 2787 1166
Size: 1621 4119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2657 2689 2657
Size: 32 5708
AlwNI CAG_nnn'CTG
Cuts at: 2027 3483 5055 2027
Size: 1456 1572 2712
Fragments arranged by size:
2712 1572 1456
ApaLI G'TGCA_C
Cuts at: 2893 3797 4953 2893
Size: 904 1156 3680
Fragments arranged by size:
3680 1156 904
ApoI r'AATT_y
Cuts at: 1493 2233 2527 3049 3275 3427 5536 1493
Size: 740 294 522 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 522 294 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2419 3065 1870
Size: 73 476 646 4545
Fragments arranged by size:
4545 646 476 73
BamHI G'GATC_C
Cuts at: 2424 3070 3437 2424
Size: 646 367 4727
Fragments arranged by size:
4727 646 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2420 3059 1391
Size: 1029 639 4072
Fragments arranged by size:
4072 1029 639
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3931 3965 1903
Size: 34 50 34 1910 34 3678
Fragments arranged by size:
3678 1910 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2752 2772 3632 4842 2752
Size: 20 860 1210 3650
Fragments arranged by size:
3650 1210 860 20
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2897 2972 3801 4957 1790
Size: 1107 75 829 1156 2573
Fragments arranged by size:
2573 1156 1107 829 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2015 3471 4429
Size: 416 53 83 399 24 598 1456 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1456 958 598 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 2471 4400 1239
Size: 1083 149 1929 2579
Fragments arranged by size:
2579 1929 1083 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2509 2728 4403 882
Size: 617 1010 219 1675 2219
Fragments arranged by size:
2219 1675 1010 617 219
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 2634 5734 2634
Size: 3100 2640
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2268 4173 4571 4845 4992 5403 2268
Size: 1905 398 274 147 411 2605
Fragments arranged by size:
2605 1905 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2531 1186
Size: 1047 298 4395
Fragments arranged by size:
4395 1047 298
BseYI C'CCAG_C
Cuts at: 2228 4943 5439 2228
Size: 2715 496 2529
Fragments arranged by size:
2715 2529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2474 2741 1163
Size: 573 630 108 267 4162
Fragments arranged by size:
4162 630 573 267 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2897 3801 3886 4957 2897
Size: 904 85 1071 3680
Fragments arranged by size:
3680 1071 904 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 2897 2972 3059 3801 3886 4957
Size: 399 630 477 75 87 742 85 1071
Cuts at: 4957 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 477 399 87 85
75
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2126 4235 4409 65
Size: 1183 878 2109 174 1396
Fragments arranged by size:
2109 1396 1183 878 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2443 4384 4571 1700
Size: 110 633 1941 187 2869
Fragments arranged by size:
2869 1941 633 187 110
BsrGI T'GTAC_A
Cuts at: 96 2472 2814 2879 96
Size: 2376 342 65 2957
Fragments arranged by size:
2957 2376 342 65
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2424 3070 3437 3822 3839 5291 5377 5389
Size: 452 646 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 646 452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2043 513
Size: 552 978 4210
Fragments arranged by size:
4210 978 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705
Size: 54 1425 50 275 18 1357 889 1629
Cuts at: 5705 8
Size: 43
Fragments arranged by size:
1629 1425 1357 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3670 1353
Size: 1373 944 3423
Fragments arranged by size:
3423 1373 944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2322 2471 2661 3803 4400 5187
Size: 393 638 52 149 190 1142 597 787
Cuts at: 5187 5455 1239
Size: 268 1524
Fragments arranged by size:
1524 1142 787 638 597 393 268 190
149 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2337 2337
Size: 5740
EcoRI G'AATT_C
Cuts at: 2233 3049 3427 2233
Size: 816 378 4546
Fragments arranged by size:
4546 816 378
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 4381 4413 4455
Size: 32 135 32 467 32 2310 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 2310 467 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4854 5038 1305
Size: 3549 184 2007
Fragments arranged by size:
3549 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2787 3698 4057 4216 518
Size: 648 843 778 911 359 159 2042
Fragments arranged by size:
2042 911 843 778 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2365 2390 3156 3833 4981 5226 1606
Size: 759 25 766 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 759 677 245 25
NcoI C'CATG_G
Cuts at: 513 1065 2043 513
Size: 552 978 4210
Fragments arranged by size:
4210 978 552
NdeI CA'TA_TG
Cuts at: 387 2566 387
Size: 2179 3561
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2296 2545 3034 3098 4643 1132
Size: 97 1067 249 489 64 1545 2229
Fragments arranged by size:
2229 1545 1067 489 249 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 1108
Size: 1338 4402
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2390 2390
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 2421 3067 1872
Size: 549 646 4545
Fragments arranged by size:
4545 646 549
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 2296 2545 3098 1132
Size: 1164 249 553 3774
Fragments arranged by size:
3774 1164 553 249
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2043 3179 513
Size: 552 978 1136 3074
Fragments arranged by size:
3074 1136 978 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 2901 3767 3784 3937 4122 4567
Size: 1255 1121 200 866 17 153 185 445
Cuts at: 4567 325
Size: 1498
Fragments arranged by size:
1498 1255 1121 866 445 200 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2814 2879
Size: 274 80 33 51 1443 495 342 65
Cuts at: 2879 3986 5698 96
Size: 1107 1712 138
Fragments arranged by size:
1712 1443 1107 495 342 274 138 80
65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 2051 1316
Size: 735 5005
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 2419 3065 1870
Size: 549 646 4545
Fragments arranged by size:
4545 646 549
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI