Restriction map for
pFN21AB3456
(Circular) (Six-base) MAPSORT of: pFN21AB3456.seq Check: 433 from: 1 to: 5782
pFN21AB3456 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3845 5229 5497 1632
Size: 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2699 1166
Size: 1533 4249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2850 3107 1870
Size: 73 907 257 4545
Fragments arranged by size:
4545 907 257 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2263 2362 2467 3101 1391
Size: 872 99 105 634 4072
Fragments arranged by size:
4072 872 634 105 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 4571
Fragments arranged by size:
4571 971 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3973 4007 1903
Size: 34 50 34 1952 34 3678
Fragments arranged by size:
3678 1952 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2418 1548
Size: 870 4912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2407 3038
Size: 107 122 71 631 605 337 398 631
Cuts at: 3038 4393 136
Size: 1355 1525
Fragments arranged by size:
1525 1355 631 631 605 398 337 122
107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2341 2482 2706 3843 4999 1790
Size: 551 141 224 1137 1156 2573
Fragments arranged by size:
2573 1156 1137 551 224 141
BmgBI CAC'GTC
Cuts at: 1318 2528 1318
Size: 1210 4572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2242 4442 1239
Size: 823 180 2200 2579
Fragments arranged by size:
2579 2200 823 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2548 3573 493
Size: 711 1344 1025 2702
Fragments arranged by size:
2702 1344 1025 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3009 3056 4215 4613 4887 5034 5445 3009
Size: 47 1159 398 274 147 411 3346
Fragments arranged by size:
3346 1159 411 398 274 147 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2056 2086 2894 2924 1178
Size: 30 165 30 653 30 808 30 4036
Fragments arranged by size:
4036 808 653 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 2662 1186
Size: 926 550 4306
Fragments arranged by size:
4306 926 550
BseYI C'CCAG_C
Cuts at: 2575 4985 5481 2575
Size: 2410 496 2876
Fragments arranged by size:
2876 2410 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2362 3843 3928 4999 2362
Size: 1481 85 1071 3145
Fragments arranged by size:
3145 1481 1071 85
BsmI GAATG_Cn'
Cuts at: 2438 3293 3386 2438
Size: 855 93 4834
Fragments arranged by size:
4834 855 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2105
Size: 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2341 2362 2467 2482 2706 3101
Size: 399 473 78 21 105 15 224 395
Cuts at: 3101 3843 3928 4999 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 399 395 224 105
85 78 21 15
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 3137 844
Size: 1453 840 3489
Fragments arranged by size:
3489 1453 840
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 3669 1394
Size: 429 559 1287 3507
Fragments arranged by size:
3507 1287 559 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 4277 4451 65
Size: 1183 1173 1856 174 1396
Fragments arranged by size:
1856 1396 1183 1173 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 4426 4613 1700
Size: 110 198 2418 187 2869
Fragments arranged by size:
2869 2418 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 2466 3842 4854 2466
Size: 1376 1012 3394
Fragments arranged by size:
3394 1376 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2791 2434
Size: 357 5425
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2985 3039 3229
Size: 54 1425 50 275 18 1155 54 190
Cuts at: 3229 4118 5747 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1155 889 275 190 54 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3712 1353
Size: 1466 893 3423
Fragments arranged by size:
3423 1466 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2360 2360
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2242 3845 4442 5229 5497 1239
Size: 393 430 180 1603 597 787 268 1524
Fragments arranged by size:
1603 1524 787 597 430 393 268 180
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 5782
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
EcoRV GAT'ATC
Cuts at: 2032 2822 2032
Size: 790 4992
FspI TGC'GCA
Cuts at: 2567 4288 2567
Size: 1721 4061
HaeII r_GCGC'y
Cuts at: 1678 2072 4929 1678
Size: 394 2857 2531
Fragments arranged by size:
2857 2531 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 4423 4455 4497
Size: 32 135 32 446 32 2373 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 2373 446 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 4896 5080 1305
Size: 783 2808 184 2007
Fragments arranged by size:
2808 2007 783 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3041 10
Size: 54 1425 343 1209 2751
Fragments arranged by size:
2751 1425 1209 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2699 3740 4099 4258 518
Size: 648 1071 462 1041 359 159 2042
Fragments arranged by size:
2042 1071 1041 648 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 2579 2946 3198 3875 5023 5268 1606
Size: 973 367 252 677 1148 245 2120
Fragments arranged by size:
2120 1148 973 677 367 252 245
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2507 3140 4685 1132
Size: 97 1278 633 1545 2229
Fragments arranged by size:
2229 1545 1278 633 97
PasI CC'CwG_GG
Cuts at: 1348 2341 1348
Size: 993 4789
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2548 2548
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2025 2091 3134 830
Size: 1195 66 1043 3478
Fragments arranged by size:
3478 1195 1043 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2579 2579
Size: 5782
SacI G_AGCT'C
Cuts at: 2362 2362
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2021 2087 3130 4265 4946 5137 826
Size: 219 976 66 1043 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 976 681 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2852 3109 1872
Size: 980 257 4545
Fragments arranged by size:
4545 980 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2205 3024 3221 513
Size: 552 1140 819 197 3074
Fragments arranged by size:
3074 1140 819 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2589 2873 2984 3809 3826 3979 4164
Size: 1255 1009 284 111 825 17 153 185
Cuts at: 4164 4609 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1009 825 445 284 185 153
111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 5742 5774 2266
Size: 32 3444 32 2274
Fragments arranged by size:
3444 2274 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2850 3107 1870
Size: 980 257 4545
Fragments arranged by size:
4545 980 257
XmnI GAAnn'nnTTC
Cuts at: 2727 3911 2727
Size: 1184 4598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI