Restriction map for
pFN21AB3446
(Circular) (Six-base) MAPSORT of: pFN21AB3446.seq Check: 4851 from: 1 to: 5590
pFN21AB3446 5590 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2646 2772 2817 3653 5037 5305 1632
Size: 656 358 126 45 836 1384 268 1917
Fragments arranged by size:
1917 1384 836 656 358 268 126 45
AfeI AGC'GCT
Cuts at: 2809 2809
Size: 5590
AflII C'TTAA_G
Cuts at: 820 1017 2051 820
Size: 197 1034 4359
Fragments arranged by size:
4359 1034 197
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2079 1166
Size: 913 4677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2670 1184
Size: 1486 4104
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2681 2899 3125 3277 5386 1493
Size: 1188 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1188 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2541 2915 1870
Size: 73 598 374 4545
Fragments arranged by size:
4545 598 374 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2564 2764 2911 4366
Size: 325 217 627 297 480 200 147 1455
Cuts at: 4366 618
Size: 1842
Fragments arranged by size:
1842 1455 627 480 325 297 217 200
147
BanII G_rGCy'C
Cuts at: 1391 2017 2509 2909 1391
Size: 626 492 400 4072
Fragments arranged by size:
4072 626 492 400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2805 2832 928
Size: 971 906 27 3686
Fragments arranged by size:
3686 971 906 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 2811 3007 2811
Size: 196 5394
Bme1580I G_kGCm'C
Cuts at: 1790 2769 3651 4807 1790
Size: 979 882 1156 2573
Fragments arranged by size:
2573 1156 979 882
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2224 2307 2824 4250 1239
Size: 985 83 517 1426 2579
Fragments arranged by size:
2579 1426 985 517 83
Bpu10I CC'TnA_GC
Cuts at: 2850 2850
Size: 5590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3715 4580 4878 2141
Size: 1574 865 298 2853
Fragments arranged by size:
2853 1574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2009 4023 4421 4695 4842 5253 2009
Size: 2014 398 274 147 411 2346
Fragments arranged by size:
2346 2014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2443 2473 1178
Size: 30 165 30 1040 30 4295
Fragments arranged by size:
4295 1040 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2451 1186
Size: 868 397 4325
Fragments arranged by size:
4325 868 397
BseYI C'CCAG_C
Cuts at: 4793 5289 4793
Size: 496 5094
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5017
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2509 3651 3736 4807 2509
Size: 1142 85 1071 3292
Fragments arranged by size:
3292 1142 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2509 2769 2909 3651 3736 4807
Size: 399 227 492 260 140 742 85 1071
Cuts at: 4807 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 492 399 260 227 140
85
BspEI T'CCGG_A
Cuts at: 2009 5253 2009
Size: 3244 2346
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2708 3477 1394
Size: 429 885 769 3507
Fragments arranged by size:
3507 885 769 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 2478 4085 4259 65
Size: 1183 1188 42 1607 174 1396
Fragments arranged by size:
1607 1396 1188 1183 174 42
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 2758 96
Size: 2662 2928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 2190 3650 4662 2190
Size: 1460 1012 3118
Fragments arranged by size:
3118 1460 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2192 2201 794
Size: 1398 9 4183
Fragments arranged by size:
4183 1398 9
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 2081
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2337 2620 2920 3287 3672 3689 5141 5227
Size: 365 283 300 367 385 17 1452 86
Cuts at: 5227 5239 5585 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 365 346 300 283
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2733 2733
Size: 5590
BtgI C'CryG_G
Cuts at: 513 1065 2360 2585 2820 513
Size: 552 1295 225 235 3283
Fragments arranged by size:
3283 1295 552 235 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2673 2718 2844 3106 3900 3920 2673
Size: 45 126 262 794 20 4343
Fragments arranged by size:
4343 794 262 126 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2281 2316 529
Size: 35 1717 35 3803
Fragments arranged by size:
3803 1717 35 35
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DraIII CAC_nnn'GTG
Cuts at: 2515 2515
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 2305 3037
Size: 54 1425 50 275 18 421 54 732
Cuts at: 3037 3926 5555 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 732 421 275 54 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2637 3520 1353
Size: 1269 15 883 3423
Fragments arranged by size:
3423 1269 883 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 5590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2288 2307 2646 2772 2817 2824
Size: 393 592 64 19 339 126 45 7
Cuts at: 2824 3653 4250 5037 5305 1239
Size: 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 592 393 339 268
126 64 45 19 7
EcoO109I rG'GnC_Cy
Cuts at: 2163 2163
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 2555 4096 2555
Size: 1541 4049
HaeII r_GCGC'y
Cuts at: 1678 2811 4737 1678
Size: 1133 1926 2531
Fragments arranged by size:
2531 1926 1133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 4231 4263 4305
Size: 32 135 32 952 32 1675 32 42
Cuts at: 4305 4337 1373
Size: 32 2626
Fragments arranged by size:
2626 1675 952 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 2934 3180 669
Size: 988 281 487 509 246 3079
Fragments arranged by size:
3079 988 509 487 281 246
HindIII A'AGCT_T
Cuts at: 748 2174 748
Size: 1426 4164
HpaI GTT'AAC
Cuts at: 2425 3180 2425
Size: 755 4835
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 2196 2298 3189 2196
Size: 102 891 4597
Fragments arranged by size:
4597 891 102
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 10
Size: 54 1425 343 421 3347
Fragments arranged by size:
3347 1425 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 3548 3907 4066 518
Size: 648 913 1469 359 159 2042
Fragments arranged by size:
2042 1469 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3006 3683 4831 5076 1606
Size: 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2585 513
Size: 552 1520 3518
Fragments arranged by size:
3518 1520 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2560 2948 4493 1132
Size: 97 1331 388 1545 2229
Fragments arranged by size:
2229 1545 1331 388 97
PasI CC'CwG_GG
Cuts at: 1348 2385 1348
Size: 1037 4553
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2303 2862 2883 1108
Size: 1195 559 21 3815
Fragments arranged by size:
3815 1195 559 21
PfoI T'CCnGG_A
Cuts at: 2240 2317 2352 2240
Size: 77 35 5478
Fragments arranged by size:
5478 77 35
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 2163
Size: 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2839 2942 830
Size: 2009 103 3478
Fragments arranged by size:
3478 2009 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SapI GCTCTTCn'nnn_
Cuts at: 2637 2637
Size: 5590
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2614 2835 2938 4073 4754 4945 826
Size: 219 1569 221 103 1135 681 191 1471
Fragments arranged by size:
1569 1471 1135 681 221 219 191 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2156 3694 4595 4857 820
Size: 197 926 108 105 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197 108 105
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2560 2948 1132
Size: 1428 388 3774
Fragments arranged by size:
3774 1428 388
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2585 2673 3029 513
Size: 552 1520 88 356 3074
Fragments arranged by size:
3074 1520 552 356 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2598 3617 3634 3787 3972 4417 325
Size: 1255 1018 1019 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1019 1018 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2607 2758
Size: 274 80 33 51 1443 135 495 151
Cuts at: 2758 3836 5548 96
Size: 1078 1712 138
Fragments arranged by size:
1712 1443 1078 495 274 151 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 5550 5582 2443
Size: 32 3075 32 2451
Fragments arranged by size:
3075 2451 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI