Restriction map for
pFN21AB3441
(Circular) (Six-base) MAPSORT of: pFN21AB3441.seq Check: 5843 from: 1 to: 5725
pFN21AB3441 5725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2759 2909 2759
Size: 150 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3024 3852 4225 1563
Size: 1461 828 373 3063
Fragments arranged by size:
3063 1461 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2607 3788 5172 5440 1632
Size: 975 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 975 268
AflII C'TTAA_G
Cuts at: 820 1017 2045 820
Size: 197 1028 4500
Fragments arranged by size:
4500 1028 197
AflIII A'CryG_T
Cuts at: 1203 2498 4624 5601 1203
Size: 1295 2126 977 1327
Fragments arranged by size:
2126 1327 1295 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 2600 4454 2600
Size: 1854 3871
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 3050 1870
Size: 73 1107 4545
Fragments arranged by size:
4545 1107 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 2850 3046 4501 618
Size: 325 217 627 376 687 196 1455 1842
Fragments arranged by size:
1842 1455 687 627 376 325 217 196
BanII G_rGCy'C
Cuts at: 1391 2787 3044 1391
Size: 1396 257 4072
Fragments arranged by size:
4072 1396 257
BbeI G_GCGC'C
Cuts at: 2854 2854
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BfrBI ATG'CAT
Cuts at: 2723 2723
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2915 4336 136
Size: 107 122 71 631 605 1243 1421 1525
Fragments arranged by size:
1525 1421 1243 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2175 3015 5720 2175
Size: 840 2705 2180
Fragments arranged by size:
2705 2180 840
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2198 2506 3786 4942 1790
Size: 376 32 308 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 376 308 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2200 3456
Size: 416 53 83 399 24 697 86 1256
Cuts at: 3456 4414 442
Size: 958 1753
Fragments arranged by size:
1753 1256 958 697 416 399 86 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2592 2592
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2508 2577 4385 1239
Size: 949 320 69 1808 2579
Fragments arranged by size:
2579 1808 949 320 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3850 4715 5013 2996
Size: 854 865 298 3708
Fragments arranged by size:
3708 865 854 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 4388 882
Size: 617 1014 1875 2219
Fragments arranged by size:
2219 1875 1014 617
BsaAI yAC'GTr
Cuts at: 493 1204 2499 3516 493
Size: 711 1295 1017 2702
Fragments arranged by size:
2702 1295 1017 711
BsaBI GATnn'nnATC
Cuts at: 2466 5719 2466
Size: 3253 2472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2745 2851 3532
Size: 53 83 186 936 531 681 106 681
Cuts at: 3532 3914 4549 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 681 681 635 531 382 186
106 83 53
BsaWI w'CCGG_w
Cuts at: 2546 4158 4556 4830 4977 5388 2546
Size: 1612 398 274 147 411 2883
Fragments arranged by size:
2883 1612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 4522
Fragments arranged by size:
4522 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2284 2846 1186
Size: 975 123 562 4065
Fragments arranged by size:
4065 975 562 123
BseYI C'CCAG_C
Cuts at: 2864 2930 4928 5424 2864
Size: 66 1998 496 3165
Fragments arranged by size:
3165 1998 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2100 1163
Size: 573 364 4788
Fragments arranged by size:
4788 573 364
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3175 3936 4964 1540
Size: 275 186 913 261 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 261 186
BsiHKAI G_wGCw'C
Cuts at: 2787 3786 3871 4942 2787
Size: 999 85 1071 3570
Fragments arranged by size:
3570 1071 999 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
BsmBI CGTCTCn'nnnn_
Cuts at: 2055 2608 2055
Size: 553 5172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2198 2506 2787 3044 3786 3871
Size: 399 376 32 308 281 257 742 85
Cuts at: 3871 4942 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 308 281 257
85 32
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 2304 2565 2787 3614 5344 2304
Size: 261 222 827 1730 2685
Fragments arranged by size:
2685 1730 827 261 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 2909 3080 844
Size: 1915 150 171 3489
Fragments arranged by size:
3489 1915 171 150
BsrBI CCG'CTC
Cuts at: 1394 1823 2847 3612 1394
Size: 429 1024 765 3507
Fragments arranged by size:
3507 1024 765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2725 4220 4394 65
Size: 1183 1477 1495 174 1396
Fragments arranged by size:
1495 1477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 2658 4369 4556 1700
Size: 110 712 136 1711 187 2869
Fragments arranged by size:
2869 1711 712 187 136 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2143 2388 2678 3785 4797 2143
Size: 245 290 1107 1012 3071
Fragments arranged by size:
3071 1107 1012 290 245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 2435 1184
Size: 1251 4474
BstYI r'GATC_y
Cuts at: 1972 2175 3015 3055 3422 3807 3824 5276 5362
Size: 203 840 40 367 385 17 1452 86
Cuts at: 5362 5374 5720 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 203 86
40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 3241 4035 4055 2153
Size: 1088 794 20 3823
Fragments arranged by size:
3823 1088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DraIII CAC_nnn'GTG
Cuts at: 2499 2499
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2945 4732 809
Size: 507 1629 1787 1802
Fragments arranged by size:
1802 1787 1629 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2524 2674
Size: 54 1425 50 275 18 339 355 150
Cuts at: 2674 3172 4061 5690 8
Size: 498 889 1629 43
Fragments arranged by size:
1629 1425 889 498 355 339 275 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3655 1353
Size: 2302 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2785 2785
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2508 2577 2607 3788 4385 5172
Size: 393 556 320 69 30 1181 597 787
Cuts at: 5172 5440 1239
Size: 268 1524
Fragments arranged by size:
1524 1181 787 597 556 393 320 268
69 30
EcoO109I rG'GnC_Cy
Cuts at: 2994 2994
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 2141 4231 2141
Size: 2090 3635
FspAI rTGC'GCAy
Cuts at: 2141 2141
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2854 4872 1678
Size: 1176 2018 2531
Fragments arranged by size:
2531 2018 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2586 2618 4366 4398 4440
Size: 32 135 32 1014 32 1748 32 42
Cuts at: 4440 4472 1373
Size: 32 2626
Fragments arranged by size:
2626 1748 1014 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2850 2850
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2676 10
Size: 54 1425 343 339 505 3059
Fragments arranged by size:
3059 1425 505 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3683 4042 4201 518
Size: 648 1815 702 359 159 2042
Fragments arranged by size:
2042 1815 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2952 3141 3818 4966 5211 1606
Size: 1346 189 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 245 189
NaeI GCC'GGC
Cuts at: 2660 2660
Size: 5725
NarI GG'CG_CC
Cuts at: 2851 2851
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 2317 387
Size: 1930 3795
NgoMIV G'CCGG_C
Cuts at: 2658 2658
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NsiI A_TGCA'T
Cuts at: 2725 2725
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2079 2656 3083 4628 1132
Size: 97 850 577 427 1545 2229
Fragments arranged by size:
2229 1545 850 577 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 2634 1108
Size: 1082 444 4199
Fragments arranged by size:
4199 1082 444
PfoI T'CCnGG_A
Cuts at: 2457 2457
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PmlI CAC'GTG
Cuts at: 2499 2499
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2994 2994
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 2756 3077 830
Size: 1926 321 3478
Fragments arranged by size:
3478 1926 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2952 2952
Size: 5725
SacI G_AGCT'C
Cuts at: 2787 2787
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 2756 3077 2756
Size: 321 5404
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2752 3073 4208 4889 5080 826
Size: 219 1707 321 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 321 219 191
SfoI GGC'GCC
Cuts at: 2852 2852
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3011 3829 4730 4992 820
Size: 197 926 102 966 818 901 262 1553
Fragments arranged by size:
1553 966 926 901 818 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 3164 513
Size: 552 2099 3074
Fragments arranged by size:
3074 2099 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 5685 5717 2062
Size: 32 3591 32 2070
Fragments arranged by size:
3591 2070 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2992 1316
Size: 1676 4049
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI