Restriction map for
pFN21AB3433
(Circular) (Six-base) MAPSORT of: pFN21AB3433.seq Check: 3013 from: 1 to: 5986
pFN21AB3433 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2650 4049 5433 5701 1632
Size: 1018 1399 1384 268 1917
Fragments arranged by size:
1917 1399 1384 1018 268
AflII C'TTAA_G
Cuts at: 820 1017 2516 820
Size: 197 1499 4290
Fragments arranged by size:
4290 1499 197
AflIII A'CryG_T
Cuts at: 1203 2286 4885 5862 1203
Size: 1083 2599 977 1327
Fragments arranged by size:
2599 1327 1083 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 3071 3230 3729 5301 3071
Size: 159 499 1572 3756
Fragments arranged by size:
3756 1572 499 159
ApaI G_GGCC'C
Cuts at: 2624 2624
Size: 5986
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2229 3295 3521 3673 5782 1493
Size: 736 1066 226 152 2109 1697
Fragments arranged by size:
2109 1697 1066 736 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2859 4540 160
Size: 872 1827 1681 1606
Fragments arranged by size:
1827 1681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 3307 4762 618
Size: 325 217 627 541 979 1455 1842
Fragments arranged by size:
1842 1455 979 627 541 325 217
BanII G_rGCy'C
Cuts at: 1391 2624 2908 3305 1391
Size: 1233 284 397 4072
Fragments arranged by size:
4072 1233 397 284
BbeI G_GCGC'C
Cuts at: 2332 2332
Size: 5986
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 928
Size: 971 599 4416
Fragments arranged by size:
4416 971 599
BbvCI CC'TCA_GC
Cuts at: 2395 2395
Size: 5986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2618 3878 5088 2618
Size: 1260 1210 3516
Fragments arranged by size:
3516 1260 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2866 5981 2866
Size: 3115 2871
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 2624 4047 5203 1790
Size: 834 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 834
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4646 1239
Size: 3407 2579
Bpu10I CC'TnA_GC
Cuts at: 2395 2395
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2989 4111 4976 5274 2807
Size: 182 1122 865 298 3519
Fragments arranged by size:
3519 1122 865 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2329 3793 4175 4810
Size: 53 83 186 936 796 1464 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1464 1451 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2775 4419 4817 5091 5238 5649 2775
Size: 1644 398 274 147 411 3112
Fragments arranged by size:
3112 1644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3281 1186
Size: 2095 3891
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2017 2917 4047 4132 5203 2017
Size: 900 1130 85 1071 2800
Fragments arranged by size:
2800 1130 1071 900 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2624 2908 2917 3305 4047 4132
Size: 399 227 607 284 9 388 742 85
Cuts at: 4132 5203 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 607 399 388 284 227
85 9
BspEI T'CCGG_A
Cuts at: 2775 5649 2775
Size: 2874 3112
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2168 3341 844
Size: 1324 1173 3489
Fragments arranged by size:
3489 1324 1173
BsrBI CCG'CTC
Cuts at: 1394 1823 2262 3873 1394
Size: 429 439 1611 3507
Fragments arranged by size:
3507 1611 439 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3071 794
Size: 2277 3709
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2866 2895 2928 3316 3683 4068 4085 5537
Size: 894 29 33 388 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 894 388 385 367 346 86
33 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2918 487
Size: 683 56 1692 3555
Fragments arranged by size:
3555 1692 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 3433 4322
Size: 54 1425 50 275 18 436 1167 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1167 889 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3916 1353
Size: 2563 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2650 4049 4646 5433 5701 1239
Size: 393 1018 1399 597 787 268 1524
Fragments arranged by size:
1524 1399 1018 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3135 3135
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2620 2620
Size: 5986
EcoRI G'AATT_C
Cuts at: 2229 3295 3673 2229
Size: 1066 378 4542
Fragments arranged by size:
4542 1066 378
EcoRV GAT'ATC
Cuts at: 2028 2542 2028
Size: 514 5472
FspI TGC'GCA
Cuts at: 2138 4492 2138
Size: 2354 3632
HaeII r_GCGC'y
Cuts at: 1678 2332 5133 1678
Size: 654 2801 2531
Fragments arranged by size:
2801 2531 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 2584 2616 4627
Size: 32 135 32 786 32 194 32 2011
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2011 786 194 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2720 748
Size: 1972 4014
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 2328 2328
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2268 10
Size: 54 1425 343 436 3728
Fragments arranged by size:
3728 1425 436 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3944 4303 4462 518
Size: 648 2778 359 159 2042
Fragments arranged by size:
2778 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3068 3080 3402 4079 5227 5472 1606
Size: 1462 12 322 677 1148 245 2120
Fragments arranged by size:
2120 1462 1148 677 322 245 12
NarI GG'CG_CC
Cuts at: 2329 2329
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 2995 387
Size: 2608 3378
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2290 2741 3344 4889 1132
Size: 97 1061 451 603 1545 2229
Fragments arranged by size:
2229 1545 1061 603 451 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 2286 4885 2286
Size: 2599 3387
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2325 1108
Size: 1217 4769
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2620 2620
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3076 3247 3286 3338 830
Size: 2246 171 39 52 3478
Fragments arranged by size:
3478 2246 171 52 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 3068 3068
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 3072 3243 3261 3282 3334 4469 5150
Size: 219 2027 171 18 21 52 1135 681
Cuts at: 5150 5341 826
Size: 191 1471
Fragments arranged by size:
2027 1471 1135 681 219 191 171 52
21 18
SfoI GGC'GCC
Cuts at: 2330 2330
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 2822 2968 4090 4991 5253
Size: 197 926 573 306 146 1122 901 262
Cuts at: 5253 820
Size: 1553
Fragments arranged by size:
1553 1122 926 901 573 306 262 197
146
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 2189 152
Size: 2037 3949
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2663 1517
Size: 1146 4840
StyI C'CwwG_G
Cuts at: 513 1065 2004 2496 3425 513
Size: 552 939 492 929 3074
Fragments arranged by size:
3074 939 929 552 492
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4030 4183 4368 4813 325
Size: 1255 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 4232 5944
Size: 274 80 33 51 1443 57 2198 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
2198 1712 1443 274 138 80 57 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2752 2784 5946 5978 2752
Size: 32 3162 32 2760
Fragments arranged by size:
3162 2760 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2276 2322 2276
Size: 46 5940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI