Restriction map for
pFN21AB3428
(Circular) (Six-base) MAPSORT of: pFN21AB3428.seq Check: 822 from: 1 to: 5974
pFN21AB3428 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 2810 3242 4037 5421 5689 1632
Size: 929 249 432 795 1384 268 1917
Fragments arranged by size:
1917 1384 929 795 432 268 249
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2067 2694 1184
Size: 883 627 4464
Fragments arranged by size:
4464 883 627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 2053
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 2512 3717 5289 2512
Size: 1205 1572 3197
Fragments arranged by size:
3197 1572 1205
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 3299 1870
Size: 73 226 1130 4545
Fragments arranged by size:
4545 1130 226 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 3295 4750 618
Size: 325 217 627 707 801 1455 1842
Fragments arranged by size:
1842 1455 801 707 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2255 3293 1391
Size: 864 1038 4072
Fragments arranged by size:
4072 1038 864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 3003 3056 928
Size: 971 317 787 53 3846
Fragments arranged by size:
3846 971 787 317 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3226 3260 4165 4199 1903
Size: 34 1289 34 905 34 3678
Fragments arranged by size:
3678 1289 905 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 2939 3391 2939
Size: 452 5522
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2834 2941 4634 1239
Size: 1118 477 107 1693 2579
Fragments arranged by size:
2579 1693 1118 477 107
Bpu10I CC'TnA_GC
Cuts at: 2216 2216
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 2838 2868 1178
Size: 30 165 30 1389 30 16 30 4284
Fragments arranged by size:
4284 1389 165 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2569 1186
Size: 1297 86 4591
Fragments arranged by size:
4591 1297 86
BseYI C'CCAG_C
Cuts at: 2667 5177 5673 2667
Size: 2510 496 2968
Fragments arranged by size:
2968 2510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2351 2711 1163
Size: 573 327 288 360 4426
Fragments arranged by size:
4426 573 360 327 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 3192 4035 4120 5191 2307
Size: 885 843 85 1071 3090
Fragments arranged by size:
3090 1071 885 843 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2307 3192 3293 4035 4120 5191
Size: 399 465 52 885 101 742 85 1071
Cuts at: 5191 1391
Size: 2174
Fragments arranged by size:
2174 1071 885 742 465 399 101 85
52
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 2787 3252 3329 844
Size: 1448 495 465 77 3489
Fragments arranged by size:
3489 1448 495 465 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 3861 1394
Size: 429 189 1849 3507
Fragments arranged by size:
3507 1849 429 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 4469 4643 65
Size: 1183 1173 2048 174 1396
Fragments arranged by size:
2048 1396 1183 1173 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2812
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2184 2661 3304 3671 4056 4073 5525 5611
Size: 212 477 643 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 643 477 385 367 346 212
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2110 3490 4284 4304 2110
Size: 1380 794 20 3780
Fragments arranged by size:
3780 1380 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2097 2418
Size: 54 1425 50 275 18 177 90 321
Cuts at: 2418 2459 3421 4310 5939 8
Size: 41 962 889 1629 43
Fragments arranged by size:
1629 1425 962 889 321 275 177 90
54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2606 3197 3904 1353
Size: 947 306 591 707 3423
Fragments arranged by size:
3423 947 707 591 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2561 2810 2834 2941 3242 4037
Size: 393 725 204 249 24 107 301 795
Cuts at: 4037 4634 5421 5689 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 795 787 725 597 393 301 268
249 204 107 24
EcoO109I rG'GnC_Cy
Cuts at: 3058 3241 3058
Size: 183 5791
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
EcoRV GAT'ATC
Cuts at: 2749 2749
Size: 5974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 4615 4647 4689
Size: 32 135 32 574 32 2437 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 2437 574 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3224 748
Size: 2476 3498
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 5088 5272 1305
Size: 792 2991 184 2007
Fragments arranged by size:
2991 2007 792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2420 10
Size: 54 1425 343 267 321 3564
Fragments arranged by size:
3564 1425 343 321 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2915 3044 3932 4291 4450 518
Size: 648 1749 129 888 359 159 2042
Fragments arranged by size:
2042 1749 888 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2725 3390 4067 5215 5460 1606
Size: 1119 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 665 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 4510
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 2524 4043 4075 5556 5588 2492
Size: 32 1519 32 1481 32 2878
Fragments arranged by size:
2878 1519 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3058 3241 3058
Size: 183 5791
PshAI GACnn'nnGTC
Cuts at: 1613 3056 1613
Size: 1443 4531
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2589 3326 830
Size: 1759 737 3478
Fragments arranged by size:
3478 1759 737
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2725 2725
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 3197 3197
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2585 2720 3322 4457 5138 5329 826
Size: 219 1540 135 602 1135 681 191 1471
Fragments arranged by size:
1540 1471 1135 681 602 219 191 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4979 5241 820
Size: 197 926 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 3413 513
Size: 552 939 1409 3074
Fragments arranged by size:
3074 1409 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2917 3163 4001 4018 4171 4356 4801
Size: 1255 1337 246 838 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1337 1255 838 445 246 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 5934 5966 2838
Size: 32 3064 32 2846
Fragments arranged by size:
3064 2846 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2447 2080
Size: 367 5607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI