Restriction map for
pFN21AB3413
(Circular) (Six-base) MAPSORT of: pFN21AB3413.seq Check: 9641 from: 1 to: 5722
pFN21AB3413 5722 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 3033 3785 5169 5437 1632
Size: 564 837 752 1384 268 1917
Fragments arranged by size:
1917 1384 837 752 564 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 2629 3465 5037 2629
Size: 836 1572 3314
Fragments arranged by size:
3314 1572 836
ApaI G_GGCC'C
Cuts at: 2347 2347
Size: 5722
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 3031 3257 3409 5518 1493
Size: 1538 226 152 2109 1697
Fragments arranged by size:
2109 1697 1538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2306 2614 3047 1870
Size: 73 363 308 433 4545
Fragments arranged by size:
4545 433 363 308 73
BamHI G'GATC_C
Cuts at: 2848 2860 3052 3419 2848
Size: 12 192 367 5151
Fragments arranged by size:
5151 367 192 12
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2478 3043 4498 618
Size: 325 217 627 691 565 1455 1842
Fragments arranged by size:
1842 1455 691 627 565 325 217
BanII G_rGCy'C
Cuts at: 1391 2347 2496 2803 3041 1391
Size: 956 149 307 238 4072
Fragments arranged by size:
4072 956 307 238 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2347 3783 4939 1790
Size: 348 209 1436 1156 2573
Fragments arranged by size:
2573 1436 1156 348 209
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 2715 3453
Size: 416 53 83 399 24 1146 152 738
Cuts at: 3453 4411 442
Size: 958 1753
Fragments arranged by size:
1753 1146 958 738 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2743 3847 4712 5010 2594
Size: 149 1104 865 298 3306
Fragments arranged by size:
3306 1104 865 298 149
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2633 2668 3529 3911
Size: 53 83 186 936 1100 35 861 382
Cuts at: 3911 4546 275
Size: 635 1451
Fragments arranged by size:
1451 1100 936 861 635 382 186 83
53 35
BsaWI w'CCGG_w
Cuts at: 2115 4155 4553 4827 4974 5385 2115
Size: 2040 398 274 147 411 2452
Fragments arranged by size:
2452 2040 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 1178
Size: 30 165 30 633 30 4834
Fragments arranged by size:
4834 633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5722
BseYI C'CCAG_C
Cuts at: 2026 4925 5421 2026
Size: 2899 496 2327
Fragments arranged by size:
2899 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3172 3933 4961 1540
Size: 275 186 115 1056 761 1028 2301
Fragments arranged by size:
2301 1056 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 2496 3783 3868 4939 2496
Size: 1287 85 1071 3279
Fragments arranged by size:
3279 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2347 2496 2803 3041 3783 3868
Size: 399 348 209 149 307 238 742 85
Cuts at: 3868 4939 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 307 238 209
149 85
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2692 2775 4217 4391 65
Size: 1183 1444 83 1442 174 1396
Fragments arranged by size:
1444 1442 1396 1183 174 83
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 2101 3782 4794 2101
Size: 1681 1012 3029
Fragments arranged by size:
3029 1681 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2154 2848 2860 3052 3419 3804 3821 5273
Size: 182 694 12 192 367 385 17 1452
Cuts at: 5273 5359 5371 5717 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 694 385 367 346 192 182
86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2082 513
Size: 552 1017 4153
Fragments arranged by size:
4153 1017 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3238 4032 4052 2977
Size: 261 794 20 4647
Fragments arranged by size:
4647 794 261 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DraIII CAC_nnn'GTG
Cuts at: 2595 2595
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2620 2805
Size: 54 1425 50 275 18 534 256 185
Cuts at: 2805 3169 4058 5687 8
Size: 364 889 1629 43
Fragments arranged by size:
1629 1425 889 534 364 275 256 185
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2538 3652 1353
Size: 1053 132 1114 3423
Fragments arranged by size:
3423 1114 1053 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2494 2494
Size: 5722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 3033 3785 4382 5169 5437 1239
Size: 393 564 837 752 597 787 268 1524
Fragments arranged by size:
1524 837 787 752 597 564 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2088 2344 2088
Size: 256 5466
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 2629 4869 1678
Size: 951 2240 2531
Fragments arranged by size:
2531 2240 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2785 2817 2840
Size: 32 135 32 743 32 438 32 23
Cuts at: 2840 2872 4363 4395 4437 4469 1373
Size: 32 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 743 438 135 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2923 3066 3312 669
Size: 988 281 985 143 246 3079
Fragments arranged by size:
3079 988 985 281 246 143
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2705 4836 5020 1305
Size: 1400 2131 184 2007
Fragments arranged by size:
2131 2007 1400 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2622 2807 10
Size: 54 1425 343 534 256 185 2925
Fragments arranged by size:
2925 1425 534 343 256 185 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 3680 4039 4198 518
Size: 648 1153 1361 359 159 2042
Fragments arranged by size:
2042 1361 1153 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2084 2151 2709 2791 3007 3138 3815
Size: 452 26 67 558 82 216 131 677
Cuts at: 3815 4963 5208 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 452 245 216 131
82 67 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2471 2706 3080 4625 1132
Size: 97 1242 235 374 1545 2229
Fragments arranged by size:
2229 1545 1242 374 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 2629 1108
Size: 1487 34 4201
Fragments arranged by size:
4201 1487 34
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 2088
Size: 5722
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PspOMI G'GGCC_C
Cuts at: 2343 2343
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2972 3074 830
Size: 2142 102 3478
Fragments arranged by size:
3478 2142 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2151 2791 2151
Size: 640 5082
SacI G_AGCT'C
Cuts at: 2496 2496
Size: 5722
SacII CC_GC'GG
Cuts at: 2085 2085
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SanDI GG'GwC_CC
Cuts at: 2088 2088
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2968 3070 4205 4886 5077 826
Size: 219 1923 102 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2573 2758 3826 4727 4989
Size: 197 926 363 267 185 1068 901 262
Cuts at: 4989 820
Size: 1553
Fragments arranged by size:
1553 1068 926 901 363 267 262 197
185
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 2706 3080 1132
Size: 1574 374 3774
Fragments arranged by size:
3774 1574 374
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 3018 1517
Size: 1501 4221
StyI C'CwwG_G
Cuts at: 513 1065 2943 3161 513
Size: 552 1878 218 3074
Fragments arranged by size:
3074 1878 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2496 3749 3766 3919 4104 4549 325
Size: 1255 916 1253 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1253 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2738 3968 5680
Size: 274 80 33 51 1443 761 1230 1712
Cuts at: 5680 96
Size: 138
Fragments arranged by size:
1712 1443 1230 761 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 5682 5714 2036
Size: 32 3614 32 2044
Fragments arranged by size:
3614 2044 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 2306 1943
Size: 363 5359
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI