Restriction map for
pFN21AB3408
(Circular) (Six-base) MAPSORT of: pFN21AB3408.seq Check: 4934 from: 1 to: 7702
pFN21AB3408 7702 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 2077
Size: 7702
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5512 278
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2883 3170 5045 1937
Size: 946 287 1875 4594
Fragments arranged by size:
4594 1875 946 287
Acc65I G'GTAC_C
Cuts at: 3161 5023 3161
Size: 1862 5840
AclI AA'CG_TT
Cuts at: 1563 5829 6202 1563
Size: 4266 373 3063
Fragments arranged by size:
4266 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 4157 4646 5765 7149 7417 1632
Size: 749 1776 489 1119 1384 268 1917
Fragments arranged by size:
1917 1776 1384 1119 749 489 268
AfeI AGC'GCT
Cuts at: 2108 3575 2108
Size: 1467 6235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7505
AflIII A'CryG_T
Cuts at: 1203 3363 6601 7578 1203
Size: 2160 3238 977 1327
Fragments arranged by size:
3238 2160 1327 977
AgeI A'CCGG_T
Cuts at: 6533 6533
Size: 7702
AhdI GACnn_n'nnGTC
Cuts at: 6431 6431
Size: 7702
AleI CACnn'nnGTG
Cuts at: 1166 3899 4178 1166
Size: 2733 279 4690
Fragments arranged by size:
4690 2733 279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 2429 3773 1184
Size: 1032 213 1344 5113
Fragments arranged by size:
5113 1344 1032 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 7670
AlwNI CAG_nnn'CTG
Cuts at: 3388 3640 4497 4553 4768 5445 7017 3388
Size: 252 857 56 215 677 1572 4073
Fragments arranged by size:
4073 1572 857 677 252 215 56
ApaLI G'TGCA_C
Cuts at: 5759 6915 5759
Size: 1156 6546
ApoI r'AATT_y
Cuts at: 1493 5011 5237 5389 7498 1493
Size: 3518 226 152 2109 1697
Fragments arranged by size:
3518 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6256 160
Size: 872 5224 1606
Fragments arranged by size:
5224 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2430 2735 2927 3157 3176 3393
Size: 73 185 302 305 192 230 19 217
Cuts at: 3393 3468 3702 3992 4476 5027 1870
Size: 75 234 290 484 551 4545
Fragments arranged by size:
4545 551 484 305 302 290 234 230
217 192 185 75 73 19
BamHI G'GATC_C
Cuts at: 2421 5032 5399 2421
Size: 2611 367 4724
Fragments arranged by size:
4724 2611 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2636 3161 3410 4896 5023
Size: 325 217 627 849 525 249 1486 127
Cuts at: 5023 6478 618
Size: 1455 1842
Fragments arranged by size:
1842 1486 1455 849 627 525 325 249
217 127
BanII G_rGCy'C
Cuts at: 1391 2081 2127 2277 2736 3131 3556 3665 4047
Size: 690 46 150 459 395 425 109 382
Cuts at: 4047 4169 4475 5021 1391
Size: 122 306 546 4072
Fragments arranged by size:
4072 690 546 459 425 395 382 306
150 122 109 46
BbeI G_GCGC'C
Cuts at: 3414 4900 3414
Size: 1486 6216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3948 4644 928
Size: 971 2049 696 3986
Fragments arranged by size:
3986 2049 971 696
BbvCI CC'TCA_GC
Cuts at: 3986 4613 3986
Size: 627 7075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3104 3138 4079 4113 5893 5927 1903
Size: 34 1167 34 941 34 1780 34 3678
Fragments arranged by size:
3678 1780 1167 941 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5594 6804 5594
Size: 1210 6492
BclI T'GATC_A
Cuts at: 1548 3873 1548
Size: 2325 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2354 2849
Size: 107 122 71 631 605 337 345 495
Cuts at: 2849 4190 4550 6313 136
Size: 1341 360 1763 1525
Fragments arranged by size:
1763 1525 1341 631 605 495 360 345
337 122 107 71
BglII A'GATC_T
Cuts at: 7697 7697
Size: 7702
BlpI GC'TnA_GC
Cuts at: 5119 5119
Size: 7702
Bme1580I G_kGCm'C
Cuts at: 1790 2431 2972 3068 5763 6919 1790
Size: 641 541 96 2695 1156 2573
Fragments arranged by size:
2695 2573 1156 641 541 96
BmgBI CAC'GTC
Cuts at: 1318 2038 3946 1318
Size: 720 1908 5074
Fragments arranged by size:
5074 1908 720
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2975 5433 6391
Size: 416 53 83 399 24 1558 2458 958
Cuts at: 6391 442
Size: 1753
Fragments arranged by size:
2458 1753 1558 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4502
Size: 7702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3479 3610 4901 6362 1239
Size: 2240 131 1291 1461 2579
Fragments arranged by size:
2579 2240 1461 1291 131
Bpu10I CC'TnA_GC
Cuts at: 2075 3664 3986 4168 4613 2075
Size: 1589 322 182 445 5164
Fragments arranged by size:
5164 1589 445 322 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4940 5827 6692 6990 4940
Size: 887 865 298 5652
Fragments arranged by size:
5652 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 3017 3465 6365 882
Size: 617 747 771 448 2900 2219
Fragments arranged by size:
2900 2219 771 747 617 448
BsaAI yAC'GTr
Cuts at: 493 1204 3168 4534 5493 493
Size: 711 1964 1366 959 2702
Fragments arranged by size:
2702 1964 1366 959 711
BsaBI GATnn'nnATC
Cuts at: 4154 7696 4154
Size: 3542 4160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2744 3411 4581 4897
Size: 53 83 186 936 1211 667 1170 316
Cuts at: 4897 5509 5891 6526 275
Size: 612 382 635 1451
Fragments arranged by size:
1451 1211 1170 936 667 635 612 382
316 186 83 53
BsaWI w'CCGG_w
Cuts at: 2709 3474 6135 6533 6807 6954 7365 2709
Size: 765 2661 398 274 147 411 3046
Fragments arranged by size:
3046 2661 765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 4211 4241 4283
Size: 30 165 30 1443 30 1335 30 42
Cuts at: 4283 4313 4518 4548 4748 4778 1178
Size: 30 205 30 200 30 4102
Fragments arranged by size:
4102 1443 1335 205 200 165 42 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2264 4262 4291 1186
Size: 1069 9 1998 29 4597
Fragments arranged by size:
4597 1998 1069 29 9
BseYI C'CCAG_C
Cuts at: 2698 6905 7401 2698
Size: 4207 496 2999
Fragments arranged by size:
4207 2999 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 2672 3019 3481 3779 4394 4817
Size: 573 699 237 347 462 298 615 423
Cuts at: 4817 1163
Size: 4048
Fragments arranged by size:
4048 699 615 573 462 423 347 298
237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2262 4179 5152 5913 6941
Size: 275 186 30 231 1917 973 761 1028
Cuts at: 6941 1540
Size: 2301
Fragments arranged by size:
2301 1917 1028 973 761 275 231 186
30
BsiHKAI G_wGCw'C
Cuts at: 2127 4634 4716 4917 5763 5848 6919 2127
Size: 2507 82 201 846 85 1071 2910
Fragments arranged by size:
2910 2507 1071 846 201 85 82
BsmI GAATG_Cn'
Cuts at: 5213 5306 5213
Size: 93 7609
BsmBI CGTCTCn'nnnn_
Cuts at: 3761 4756 3761
Size: 995 6707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2127 2277 2431 2736 2972 3068
Size: 399 291 46 150 154 305 236 96
Cuts at: 3068 3131 3556 3665 4047 4169 4475 4634 4716
Size: 63 425 109 382 122 306 159 82
Cuts at: 4716 4917 5021 5763 5848 6919 1391
Size: 201 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 382 306 305
291 236 201 159 154 150 122 109
104 96 85 82 63 46
BspEI T'CCGG_A
Cuts at: 2709 7365 2709
Size: 4656 3046
BspHI T'CATG_A
Cuts at: 3870 5591 7321 3870
Size: 1721 1730 4251
Fragments arranged by size:
4251 1730 1721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 3387 4083 4420 5057 844
Size: 1233 1310 696 337 637 3489
Fragments arranged by size:
3489 1310 1233 696 637 337
BsrBI CCG'CTC
Cuts at: 1394 1823 2397 2740 4648 4893 5589 1394
Size: 429 574 343 1908 245 696 3507
Fragments arranged by size:
3507 1908 696 574 429 343 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 4741 6197 6371 65
Size: 1183 3493 1456 174 1396
Fragments arranged by size:
3493 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 2681 4106 4655 6346 6533 1700
Size: 110 283 588 1425 549 1691 187 2869
Fragments arranged by size:
2869 1691 1425 588 549 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7702
BssHII G'CGCG_C
Cuts at: 1925 2097 1925
Size: 172 7530
BssSI C'ACGA_G
Cuts at: 2297 3143 4715 4850 5762 6774 2297
Size: 846 1572 135 912 1012 3225
Fragments arranged by size:
3225 1572 1012 912 846 135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3439 794
Size: 2645 5057
BstBI TT'CG_AA
Cuts at: 6506 6506
Size: 7702
BstEII G'GTnAC_C
Cuts at: 1184 2089 4879 1184
Size: 905 2790 4007
Fragments arranged by size:
4007 2790 905
BstXI CCAn_nnnn'nTGG
Cuts at: 4423 4699 4825 4423
Size: 276 126 7300
Fragments arranged by size:
7300 276 126
BstYI r'GATC_y
Cuts at: 1972 2421 2706 3636 3648 5032 5399 5784 5801
Size: 449 285 930 12 1384 367 385 17
Cuts at: 5801 7253 7339 7351 7697 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1384 930 449 385 367 346
285 86 17 12 12
BstZ17I GTA'TAC
Cuts at: 3171 3171
Size: 7702
Bsu36I CC'TnA_GG
Cuts at: 3819 3819
Size: 7702
BtgI C'CryG_G
Cuts at: 513 1065 2004 2673 2854 2901 3018 3324 3900
Size: 552 939 669 181 47 117 306 576
Cuts at: 3900 4161 4179 4416 4698 4824 513
Size: 261 18 237 282 126 3391
Fragments arranged by size:
3391 939 669 576 552 306 282 261
237 181 126 117 47 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4125 4141 487
Size: 683 56 2899 16 4048
Fragments arranged by size:
4048 2899 683 56 16
BtsI GCAGTG_nn'
Cuts at: 2472 3894 4095 5218 6012 6032 2472
Size: 1422 201 1123 794 20 4142
Fragments arranged by size:
4142 1422 1123 794 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7667
DraI TTT'AAA
Cuts at: 5006 5006
Size: 7702
DraIII CAC_nnn'GTG
Cuts at: 4566 4566
Size: 7702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6709 809
Size: 507 5393 1802
Fragments arranged by size:
5393 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2301 2670
Size: 54 1425 50 275 18 198 273 369
Cuts at: 2670 2676 2811 2833 2919 3217 3523 3696 3903
Size: 6 135 22 86 298 306 173 207
Cuts at: 3903 4176 4182 4542 4815 5149 6038 7667 8
Size: 273 6 360 273 334 889 1629 43
Fragments arranged by size:
1629 1425 889 369 360 334 306 298
275 273 273 273 207 198 173 135
86 54 50 43 22 18 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 2028 4176 5149 1537
Size: 275 216 2148 973 4090
Fragments arranged by size:
4090 2148 973 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 4508 5632 1353
Size: 3155 1124 3423
Fragments arranged by size:
3423 3155 1124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 6289 6663 6809 387
Size: 1064 138 894 3806 374 146 1280
Fragments arranged by size:
3806 1280 1064 894 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2125
Size: 7702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 3479 3610 4157 4646 4901 5765
Size: 393 749 1098 131 547 489 255 864
Cuts at: 5765 6362 7149 7417 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1098 864 787 749 597 547 489
393 268 255 131
EcoO109I rG'GnC_Cy
Cuts at: 2179 2356 2932 4589 4951 2179
Size: 177 576 1657 362 4930
Fragments arranged by size:
4930 1657 576 362 177
EcoRI G'AATT_C
Cuts at: 5011 5389 5011
Size: 378 7324
EcoRV GAT'ATC
Cuts at: 4923 4923
Size: 7702
FspI TGC'GCA
Cuts at: 2192 6208 2192
Size: 4016 3686
FspAI rTGC'GCAy
Cuts at: 2192 2192
Size: 7702
HaeII r_GCGC'y
Cuts at: 1678 2110 2880 3414 3577 4006 4900 6849 1678
Size: 432 770 534 163 429 894 1949 2531
Fragments arranged by size:
2531 1949 894 770 534 432 429 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 3113 3145 3464
Size: 32 135 32 923 32 586 32 319
Cuts at: 3464 3496 3647 3679 4581 4613 4748 4780 6343
Size: 32 151 32 902 32 135 32 1563
Cuts at: 6343 6375 6417 6449 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1563 923 902 586 319 151 135
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5046 5292 669
Size: 988 281 3108 246 3079
Fragments arranged by size:
3108 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7702
HpaI GTT'AAC
Cuts at: 5292 5292
Size: 7702
KasI G'GCGC_C
Cuts at: 3410 4896 3410
Size: 1486 6216
KpnI G_GTAC'C
Cuts at: 3165 5027 3165
Size: 1862 5840
MfeI C'AATT_G
Cuts at: 5301 5301
Size: 7702
MluI A'CGCG_T
Cuts at: 7578 7578
Size: 7702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3078 6816 7000 1305
Size: 1773 3738 184 2007
Fragments arranged by size:
3738 2007 1773 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2678 3525 3698 3905
Size: 54 1425 343 471 375 847 173 207
Cuts at: 3905 4184 4544 4817 10
Size: 279 360 273 2895
Fragments arranged by size:
2895 1425 847 471 375 360 343 279
273 207 173 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 3488 3863 3899 4100 4160 4178
Size: 648 1632 690 375 36 201 60 18
Cuts at: 4178 4697 4727 4823 5660 6019 6178 518
Size: 519 30 96 837 359 159 2042
Fragments arranged by size:
2042 1632 837 690 648 519 375 359
201 159 96 60 36 30 18
MspA1I CmG'CkG
Cuts at: 1606 2749 2782 3436 4026 4378 4612 5118 5795
Size: 1143 33 654 590 352 234 506 677
Cuts at: 5795 6943 7188 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1143 677 654 590 506 352
245 234 33
NaeI GCC'GGC
Cuts at: 4657 4657
Size: 7702
NarI GG'CG_CC
Cuts at: 3411 4897 3411
Size: 1486 6216
NcoI C'CATG_G
Cuts at: 513 1065 2004 4161 4698 4824 513
Size: 552 939 2157 537 126 3391
Fragments arranged by size:
3391 2157 939 552 537 126
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7702
NgoMIV G'CCGG_C
Cuts at: 4655 4655
Size: 7702
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7702
NotI GC'GGCC_GC
Cuts at: 5149 5149
Size: 7702
NspI r_CATG'y
Cuts at: 1132 1229 2626 2958 3802 4981 5060 6605 1132
Size: 97 1397 332 844 1179 79 1545 2229
Fragments arranged by size:
2229 1545 1397 1179 844 332 97 79
PasI CC'CwG_GG
Cuts at: 1348 2444 3028 1348
Size: 1096 584 6022
Fragments arranged by size:
6022 1096 584
PciI A'CATG_T
Cuts at: 6601 6601
Size: 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4566 4906 1108
Size: 3458 340 3904
Fragments arranged by size:
3904 3458 340
PfoI T'CCnGG_A
Cuts at: 3651 4967 3651
Size: 1316 6386
PmeI GTTT'AAAC
Cuts at: 5006 5006
Size: 7702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5771 5803 7284 7316 5771
Size: 32 1481 32 6157
Fragments arranged by size:
6157 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 4589 2179
Size: 2410 5292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7702
PsiI TTA'TAA
Cuts at: 5272 5272
Size: 7702
PstI C_TGCA'G
Cuts at: 830 2754 3384 3749 4080 5054 830
Size: 1924 630 365 331 974 3478
Fragments arranged by size:
3478 1924 974 630 365 331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7702
PvuII CAG'CTG
Cuts at: 2782 3436 4378 4612 2782
Size: 654 942 234 5872
Fragments arranged by size:
5872 942 654 234
RsrII CG'GwC_CG
Cuts at: 2401 2401
Size: 7702
SacI G_AGCT'C
Cuts at: 2127 2127
Size: 7702
SalI G'TCGA_C
Cuts at: 1936 5044 1936
Size: 3108 4594
SapI GCTCTTCn'nnn_
Cuts at: 4508 4508
Size: 7702
SbfI CC_TGCA'GG
Cuts at: 3384 5054 3384
Size: 1670 6032
ScaI AGT'ACT
Cuts at: 5950 7662 5950
Size: 1712 5990
SexAI A'CCwGG_T
Cuts at: 4392 4562 4392
Size: 170 7532
SfcI C'TryA_G
Cuts at: 826 1045 2750 3380 3577 3745 4076 4264 5050
Size: 219 1705 630 197 168 331 188 786
Cuts at: 5050 6185 6866 7057 826
Size: 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 786 681 630 331 219
197 191 188 168
SfoI GGC'GCC
Cuts at: 3412 4898 3412
Size: 1486 6216
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7702
SmaI CCC'GGG
Cuts at: 1872 2432 3395 3704 5029 1872
Size: 560 963 309 1325 4545
Fragments arranged by size:
4545 1325 963 560 309
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4955 5806 6707 6969 820
Size: 197 926 3012 851 901 262 1553
Fragments arranged by size:
3012 1553 926 901 851 262 197
SnaBI TAC'GTA
Cuts at: 493 4534 5493 493
Size: 4041 959 2702
Fragments arranged by size:
4041 2702 959
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7702
SphI G_CATG'C
Cuts at: 1132 3802 5060 1132
Size: 2670 1258 3774
Fragments arranged by size:
3774 2670 1258
SspI AAT'ATT
Cuts at: 5 52 5626 5
Size: 47 5574 2081
Fragments arranged by size:
5574 2081 47
StuI AGG'CCT
Cuts at: 1517 3959 4010 4539 1517
Size: 2442 51 529 4680
Fragments arranged by size:
4680 2442 529 51
StyI C'CwwG_G
Cuts at: 513 1065 2004 3294 3716 4161 4698 4824 5141
Size: 552 939 1290 422 445 537 126 317
Cuts at: 5141 513
Size: 3074
Fragments arranged by size:
3074 1290 939 552 537 445 422 317
126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2654 4375 4894 5729 5746 5899 6084
Size: 1255 1074 1721 519 835 17 153 185
Cuts at: 6084 6529 325
Size: 445 1498
Fragments arranged by size:
1721 1498 1255 1074 835 519 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3492 5948 7660
Size: 274 80 33 51 1443 1515 2456 1712
Cuts at: 7660 96
Size: 138
Fragments arranged by size:
2456 1712 1515 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 7662 7694 2846
Size: 32 4784 32 2854
Fragments arranged by size:
4784 2854 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2438 1316
Size: 1122 6580
XbaI T'CTAG_A
Cuts at: 5038 5038
Size: 7702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2524 2311
Size: 213 7489
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7702
XmaI C'CCGG_G
Cuts at: 1870 2430 3393 3702 5027 1870
Size: 560 963 309 1325 4545
Fragments arranged by size:
4545 1325 963 560 309
XmnI GAAnn'nnTTC
Cuts at: 3365 3449 4280 5831 3365
Size: 84 831 1551 5236
Fragments arranged by size:
5236 1551 831 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5510 276
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI EcoNI FalI
FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI
SacII SanDI SfiI SrfI SwaI