Restriction map for
pFN21AB3407
(Circular) (Six-base) MAPSORT of: pFN21AB3407.seq Check: 6270 from: 1 to: 8545
pFN21AB3407 8545 bp
With 182 enzymes: SgfI *
October 14, 2014 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5690 5690
Size: 8545
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3795 6355 278
Size: 53 83 186 936 2259 2560 2468
Fragments arranged by size:
2560 2468 2259 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 4078 5888 1937
Size: 1836 305 1810 4594
Fragments arranged by size:
4594 1836 1810 305
Acc65I G'GTAC_C
Cuts at: 4780 5866 4780
Size: 1086 7459
AclI AA'CG_TT
Cuts at: 1563 6672 7045 1563
Size: 5109 373 3063
Fragments arranged by size:
5109 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2787 3184 3269 4304 4719 5147 6608 7992
Size: 1155 397 85 1035 415 428 1461 1384
Cuts at: 7992 8260 1632
Size: 268 1917
Fragments arranged by size:
1917 1461 1384 1155 1035 428 415 397
268 85
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8348
AflIII A'CryG_T
Cuts at: 1203 2630 7444 8421 1203
Size: 1427 4814 977 1327
Fragments arranged by size:
4814 1427 1327 977
AgeI A'CCGG_T
Cuts at: 7376 7376
Size: 8545
AhdI GACnn_n'nnGTC
Cuts at: 7274 7274
Size: 8545
AleI CACnn'nnGTG
Cuts at: 1166 3752 1166
Size: 2586 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8545
AlwNI CAG_nnn'CTG
Cuts at: 2693 2765 3294 5170 5200 5223 5251 5782 5785
Size: 72 529 1876 30 23 28 531 3
Cuts at: 5785 6288 7860 2693
Size: 503 1572 3378
Fragments arranged by size:
3378 1876 1572 531 529 503 72 30
28 23 3
ApaI G_GGCC'C
Cuts at: 3592 3592
Size: 8545
ApaLI G'TGCA_C
Cuts at: 2498 6602 7758 2498
Size: 4104 1156 3285
Fragments arranged by size:
4104 3285 1156
ApoI r'AATT_y
Cuts at: 1493 2254 2842 3175 3182 3560 4114 5854 6080
Size: 761 588 333 7 378 554 1740 226
Cuts at: 6080 6232 8341 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1740 1697 761 588 554 378 333
226 152 7
AseI AT'TA_AT
Cuts at: 160 1032 2550 3020 7099 160
Size: 872 1518 470 4079 1606
Fragments arranged by size:
4079 1606 1518 872 470
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
AvaI C'yCGr_G
Cuts at: 1870 1943 5870 1870
Size: 73 3927 4545
Fragments arranged by size:
4545 3927 73
BamHI G'GATC_C
Cuts at: 5875 6242 5875
Size: 367 8178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2412 4780 4955 5866 7321
Size: 325 217 627 625 2368 175 911 1455
Cuts at: 7321 618
Size: 1842
Fragments arranged by size:
2368 1842 1455 911 627 625 325 217
175
BanII G_rGCy'C
Cuts at: 1391 3592 5364 5864 1391
Size: 2201 1772 500 4072
Fragments arranged by size:
4072 2201 1772 500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 2434 3957 928
Size: 971 438 97 1523 5516
Fragments arranged by size:
5516 1523 971 438 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6736 6770 1903
Size: 34 4799 34 3678
Fragments arranged by size:
4799 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5617 6437 7647 5617
Size: 820 1210 6515
Fragments arranged by size:
6515 1210 820
BclI T'GATC_A
Cuts at: 1548 5034 1548
Size: 3486 5059
BfrBI ATG'CAT
Cuts at: 2927 2927
Size: 8545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7156 136
Size: 107 122 71 631 605 5484 1525
Fragments arranged by size:
5484 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2263 3228 8540 2263
Size: 965 5312 2268
Fragments arranged by size:
5312 2268 965
BlpI GC'TnA_GC
Cuts at: 5962 5962
Size: 8545
Bme1580I G_kGCm'C
Cuts at: 1790 2502 3153 3592 3637 5572 6606 7762 1790
Size: 712 651 439 45 1935 1034 1156 2573
Fragments arranged by size:
2573 1935 1156 1034 712 651 439 45
BmgBI CAC'GTC
Cuts at: 1318 3678 5613 1318
Size: 2360 1935 4250
Fragments arranged by size:
4250 2360 1935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2619 3586 5150
Size: 416 53 83 399 24 1202 967 1564
Cuts at: 5150 6276 7234 442
Size: 1126 958 1753
Fragments arranged by size:
1753 1564 1202 1126 967 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4370
Size: 8545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2775 4068 4365 4458 5198 5273 7205
Size: 1106 430 1293 297 93 740 75 1932
Cuts at: 7205 1239
Size: 2579
Fragments arranged by size:
2579 1932 1293 1106 740 430 297 93
75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4458 6670 7535 7833 4458
Size: 2212 865 298 5170
Fragments arranged by size:
5170 2212 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7208 882
Size: 617 5709 2219
Fragments arranged by size:
5709 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2633 3632 6336 493
Size: 711 1429 999 2704 2702
Fragments arranged by size:
2704 2702 1429 999 711
BsaBI GATnn'nnATC
Cuts at: 8539 8539
Size: 8545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3792 6352 6734 7369
Size: 53 83 186 936 2259 2560 382 635
Cuts at: 7369 275
Size: 1451
Fragments arranged by size:
2560 2259 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4875 5146 6978 7376 7650 7797 8208 4875
Size: 271 1832 398 274 147 411 5212
Fragments arranged by size:
5212 1832 411 398 274 271 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3807 3837 4006 4036 4733
Size: 30 165 30 2404 30 169 30 697
Cuts at: 4733 4763 1178
Size: 30 4960
Fragments arranged by size:
4960 2404 697 169 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2046 3096 1186
Size: 860 1050 6635
Fragments arranged by size:
6635 1050 860
BseYI C'CCAG_C
Cuts at: 2372 4682 7748 8244 2372
Size: 2310 3066 496 2673
Fragments arranged by size:
3066 2673 2310 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3275 1163
Size: 573 1539 6433
Fragments arranged by size:
6433 1539 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5147 5995 6756 7784 1540
Size: 275 186 3146 848 761 1028 2301
Fragments arranged by size:
3146 2301 1028 848 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2502 3308 5237 5338 6606 6691 7762 2502
Size: 806 1929 101 1268 85 1071 3285
Fragments arranged by size:
3285 1929 1268 1071 806 101 85
BsmI GAATG_Cn'
Cuts at: 5725 6056 6149 5725
Size: 331 93 8121
Fragments arranged by size:
8121 331 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 3153 3308 3592 3637 5237 5338
Size: 399 712 651 155 284 45 1600 101
Cuts at: 5338 5364 5572 5864 6606 6691 7762 1391
Size: 26 208 292 742 85 1071 2174
Fragments arranged by size:
2174 1600 1071 742 712 651 399 292
284 208 155 101 85 45 26
BspEI T'CCGG_A
Cuts at: 8208 8208
Size: 8545
BspHI T'CATG_A
Cuts at: 2205 2316 3543 3838 3849 4908 5031 6434 8164
Size: 111 1227 295 11 1059 123 1403 1730
Cuts at: 8164 2205
Size: 2586
Fragments arranged by size:
2586 1730 1403 1227 1059 295 123 111
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4768 5690 5900 844
Size: 3924 922 210 3489
Fragments arranged by size:
3924 3489 922 210
BsrBI CCG'CTC
Cuts at: 1394 1823 5257 6432 1394
Size: 429 3434 1175 3507
Fragments arranged by size:
3507 3434 1175 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2032 3624 4605 4851 5382 5394 5553
Size: 1183 784 1592 981 246 531 12 159
Cuts at: 5553 7040 7214 65
Size: 1487 174 1396
Fragments arranged by size:
1592 1487 1396 1183 981 784 531 246
174 159 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 7189 7376 1700
Size: 110 5379 187 2869
Fragments arranged by size:
5379 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8545
BssSI C'ACGA_G
Cuts at: 5565 6605 7617 5565
Size: 1040 1012 6493
Fragments arranged by size:
6493 1040 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2933 794
Size: 2139 6406
BstBI TT'CG_AA
Cuts at: 3140 7349 3140
Size: 4209 4336
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8545
BstXI CCAn_nnnn'nTGG
Cuts at: 2192 3599 4966 2192
Size: 1407 1367 5771
Fragments arranged by size:
5771 1407 1367
BstYI r'GATC_y
Cuts at: 1972 2008 2263 3228 3654 3980 4350 5875 6242
Size: 36 255 965 426 326 370 1525 367
Cuts at: 6242 6627 6644 8096 8182 8194 8540 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1525 1452 965 426 385 370 367
346 326 255 86 36 17 12
BstZ17I GTA'TAC
Cuts at: 4079 4079
Size: 8545
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 3492 4021 4959 4965 513
Size: 552 939 181 1307 529 938 6 4093
Fragments arranged by size:
4093 1307 939 938 552 529 181 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3781 487
Size: 683 56 2555 5251
Fragments arranged by size:
5251 2555 683 56
BtsI GCAGTG_nn'
Cuts at: 3711 5224 5483 6061 6855 6875 3711
Size: 1513 259 578 794 20 5381
Fragments arranged by size:
5381 1513 794 578 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8510
DraI TTT'AAA
Cuts at: 2056 5849 2056
Size: 3793 4752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7552 809
Size: 507 6236 1802
Fragments arranged by size:
6236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2661 3475 4962
Size: 54 1425 50 275 18 831 814 1487
Cuts at: 4962 5539 5992 6881 8510 8
Size: 577 453 889 1629 43
Fragments arranged by size:
1629 1487 1425 889 831 814 577 453
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5992 1537
Size: 275 4180 4090
Fragments arranged by size:
4180 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 3137 3296 5225 5427 6475 1353
Size: 1410 374 159 1929 202 1048 3423
Fragments arranged by size:
3423 1929 1410 1048 374 202 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7132 7506 7652 387
Size: 1064 138 5543 374 146 1280
Fragments arranged by size:
5543 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2775 2787 3184 3269 4068 4304
Size: 393 713 430 12 397 85 799 236
Cuts at: 4304 4365 4458 4719 5147 5198 5273 6608 7205
Size: 61 93 261 428 51 75 1335 597
Cuts at: 7205 7992 8260 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1335 799 787 713 597 430 428
397 393 268 261 236 93 85 75
61 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4096 4096
Size: 8545
EcoO109I rG'GnC_Cy
Cuts at: 3238 3588 3238
Size: 350 8195
EcoRI G'AATT_C
Cuts at: 3182 5854 6232 3182
Size: 2672 378 5495
Fragments arranged by size:
5495 2672 378
EcoRV GAT'ATC
Cuts at: 2236 2296 2236
Size: 60 8485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 5306 2065
Size: 3241 5304
FspI TGC'GCA
Cuts at: 7051 7051
Size: 8545
HaeII r_GCGC'y
Cuts at: 1678 4683 5842 7692 1678
Size: 3005 1159 1850 2531
Fragments arranged by size:
3005 2531 1850 1159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2187 2219 2255 2287 2525
Size: 32 135 32 615 32 36 32 238
Cuts at: 2525 2557 2982 3014 3662 3694 3748 3780 3965
Size: 32 425 32 648 32 54 32 185
Cuts at: 3965 3997 4377 4409 5136 5168 5188 5220 7186
Size: 32 380 32 727 32 20 32 1966
Cuts at: 7186 7218 7260 7292 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1966 727 648 615 425 380 238
185 135 54 42 36 32 32 32
32 32 32 32 32 32 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 5889 6135 669
Size: 988 281 3951 246 3079
Fragments arranged by size:
3951 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4046 748
Size: 3298 5247
HpaI GTT'AAC
Cuts at: 6135 6135
Size: 8545
KpnI G_GTAC'C
Cuts at: 4784 5870 4784
Size: 1086 7459
MfeI C'AATT_G
Cuts at: 4593 6144 4593
Size: 1551 6994
MluI A'CGCG_T
Cuts at: 8421 8421
Size: 8545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2618 3019 3262 3556 3722 7659 7843 1305
Size: 1313 401 243 294 166 3937 184 2007
Fragments arranged by size:
3937 2007 1313 401 294 243 184 166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 5541 10
Size: 54 1425 343 831 2878 3014
Fragments arranged by size:
3014 2878 1425 831 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2410 3416 3752 4761 4964 5030 5156
Size: 648 1244 1006 336 1009 203 66 126
Cuts at: 5156 6503 6862 7021 518
Size: 1347 359 159 2042
Fragments arranged by size:
2042 1347 1244 1009 1006 648 359 336
203 159 126 66
MspA1I CmG'CkG
Cuts at: 1606 2423 2690 3319 3926 4943 5782 5961 6638
Size: 817 267 629 607 1017 839 179 677
Cuts at: 6638 7786 8031 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1017 839 817 677 629 607
267 245 179
NcoI C'CATG_G
Cuts at: 513 1065 2004 2185 3492 4959 513
Size: 552 939 181 1307 1467 4099
Fragments arranged by size:
4099 1467 1307 939 552 181
NdeI CA'TA_TG
Cuts at: 387 4758 387
Size: 4371 4174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8545
NotI GC'GGCC_GC
Cuts at: 5992 5992
Size: 8545
NsiI A_TGCA'T
Cuts at: 2929 2929
Size: 8545
NspI r_CATG'y
Cuts at: 1132 1229 5471 5903 7448 1132
Size: 97 4242 432 1545 2229
Fragments arranged by size:
4242 2229 1545 432 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8545
PciI A'CATG_T
Cuts at: 7444 7444
Size: 8545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5200 5251 1108
Size: 4092 51 4402
Fragments arranged by size:
4402 4092 51
PfoI T'CCnGG_A
Cuts at: 5250 5250
Size: 8545
PmeI GTTT'AAAC
Cuts at: 5849 5849
Size: 8545
PmlI CAC'GTG
Cuts at: 2633 3632 2633
Size: 999 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6614 6646 8127 8159 6614
Size: 32 1481 32 7000
Fragments arranged by size:
7000 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8545
PsiI TTA'TAA
Cuts at: 4163 6115 4163
Size: 1952 6593
PspOMI G'GGCC_C
Cuts at: 3588 3588
Size: 8545
PstI C_TGCA'G
Cuts at: 830 5381 5897 830
Size: 4551 516 3478
Fragments arranged by size:
4551 3478 516
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8545
PvuII CAG'CTG
Cuts at: 2423 2690 3319 3926 4943 5782 2423
Size: 267 629 607 1017 839 5186
Fragments arranged by size:
5186 1017 839 629 607 267
SalI G'TCGA_C
Cuts at: 1936 5887 1936
Size: 3951 4594
SapI GCTCTTCn'nnn_
Cuts at: 3296 5225 3296
Size: 1929 6616
SbfI CC_TGCA'GG
Cuts at: 5897 5897
Size: 8545
ScaI AGT'ACT
Cuts at: 2997 6793 8505 2997
Size: 3796 1712 3037
Fragments arranged by size:
3796 3037 1712
SexAI A'CCwGG_T
Cuts at: 2339 2339
Size: 8545
SfcI C'TryA_G
Cuts at: 826 1045 2898 3774 4488 5065 5377 5893 7028
Size: 219 1853 876 714 577 312 516 1135
Cuts at: 7028 7709 7900 826
Size: 681 191 1471
Fragments arranged by size:
1853 1471 1135 876 714 681 577 516
312 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8545
SmaI CCC'GGG
Cuts at: 1872 5872 1872
Size: 4000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4473 6649 7550 7812 820
Size: 197 926 2530 2176 901 262 1553
Fragments arranged by size:
2530 2176 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6336 493
Size: 5843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8545
SphI G_CATG'C
Cuts at: 1132 5903 1132
Size: 4771 3774
SspI AAT'ATT
Cuts at: 5 52 2893 2981 3166 4197 6469 5
Size: 47 2841 88 185 1031 2272 2081
Fragments arranged by size:
2841 2272 2081 1031 185 88 47
StuI AGG'CCT
Cuts at: 1517 4173 5079 1517
Size: 2656 906 4983
Fragments arranged by size:
4983 2656 906
StyI C'CwwG_G
Cuts at: 513 1065 2004 2185 2961 3492 3684 3954 4288
Size: 552 939 181 776 531 192 270 334
Cuts at: 4288 4959 5271 5984 513
Size: 671 312 713 3074
Fragments arranged by size:
3074 939 776 713 671 552 531 334
312 270 192 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4853 6572 6589 6742 6927 7372 325
Size: 1255 3273 1719 17 153 185 445 1498
Fragments arranged by size:
3273 1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2995 6791 8503
Size: 274 80 33 51 1443 1018 3796 1712
Cuts at: 8503 96
Size: 138
Fragments arranged by size:
3796 1712 1443 1018 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 3807 3839 4006 4038 5690 5722 8505
Size: 32 432 32 167 32 1652 32 2783
Cuts at: 8505 8537 3343
Size: 32 3351
Fragments arranged by size:
3351 2783 1652 432 167 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8545
XbaI T'CTAG_A
Cuts at: 5881 5881
Size: 8545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3349 3599 3682 4063 4092 5617 3349
Size: 250 83 381 29 1525 6277
Fragments arranged by size:
6277 1525 381 250 83 29
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8545
XmaI C'CCGG_G
Cuts at: 1870 5870 1870
Size: 4000 4545
XmnI GAAnn'nnTTC
Cuts at: 3465 4297 6674 3465
Size: 832 2377 5336
Fragments arranged by size:
5336 2377 832
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3793 6353 276
Size: 53 83 186 936 2259 2560 2468
Fragments arranged by size:
2560 2468 2259 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI