Restriction map for
pFN21AB3404
(Circular) (Six-base) MAPSORT of: pFN21AB3404.seq Check: 1565 from: 1 to: 9778
pFN21AB3404 9778 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7588 278
Size: 53 83 186 936 6052 2468
Fragments arranged by size:
6052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4387 7121 1937
Size: 2450 2734 4594
Fragments arranged by size:
4594 2734 2450
Acc65I G'GTAC_C
Cuts at: 7099 7099
Size: 9778
AclI AA'CG_TT
Cuts at: 1563 3620 7905 8278 1563
Size: 2057 4285 373 3063
Fragments arranged by size:
4285 3063 2057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 2547 2696 3348 3945 4022 4344 4502
Size: 433 482 149 652 597 77 322 158
Cuts at: 4502 4563 4830 5259 5968 6502 7841 9225 9493
Size: 61 267 429 709 534 1339 1384 268
Cuts at: 9493 1632
Size: 1917
Fragments arranged by size:
1917 1384 1339 709 652 597 534 482
433 429 322 268 267 158 149 77
61
AflII C'TTAA_G
Cuts at: 820 1017 3853 4157 4381 820
Size: 197 2836 304 224 6217
Fragments arranged by size:
6217 2836 304 224 197
AflIII A'CryG_T
Cuts at: 1203 5638 8677 9654 1203
Size: 4435 3039 977 1327
Fragments arranged by size:
4435 3039 1327 977
AgeI A'CCGG_T
Cuts at: 8609 8609
Size: 9778
AhdI GACnn_n'nnGTC
Cuts at: 2800 5091 8507 2800
Size: 2291 3416 4071
Fragments arranged by size:
4071 3416 2291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9778
AlwNI CAG_nnn'CTG
Cuts at: 2714 5435 6655 7521 9093 2714
Size: 2721 1220 866 1572 3399
Fragments arranged by size:
3399 2721 1572 1220 866
ApaI G_GGCC'C
Cuts at: 2058 2058
Size: 9778
ApaLI G'TGCA_C
Cuts at: 7835 8991 7835
Size: 1156 8622
ApoI r'AATT_y
Cuts at: 1493 2507 3112 3142 3737 4271 4564 5300 5501
Size: 1014 605 30 595 534 293 736 201
Cuts at: 5501 5936 5946 6827 7087 7313 7465 9574 1493
Size: 435 10 881 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 881 736 605 595 534
435 293 260 226 201 152 30 10
AseI AT'TA_AT
Cuts at: 160 1032 6404 6590 8332 160
Size: 872 5372 186 1742 1606
Fragments arranged by size:
5372 1742 1606 872 186
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9778
AvaI C'yCGr_G
Cuts at: 1870 1943 7103 1870
Size: 73 5160 4545
Fragments arranged by size:
5160 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4496 4529 4496
Size: 33 9745
BamHI G'GATC_C
Cuts at: 7108 7475 7108
Size: 367 9411
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7099 8554 618
Size: 325 217 627 5312 1455 1842
Fragments arranged by size:
5312 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 5860 6887 7097 1391
Size: 667 3802 1027 210 4072
Fragments arranged by size:
4072 3802 1027 667 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 3015 928
Size: 971 336 780 7691
Fragments arranged by size:
7691 971 780 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7969 8003 1903
Size: 34 6032 34 3678
Fragments arranged by size:
6032 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7670 8880 7670
Size: 1210 8568
BclI T'GATC_A
Cuts at: 1548 5710 1548
Size: 4162 5616
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4685 8389 136
Size: 107 122 71 631 605 3013 3704 1525
Fragments arranged by size:
3704 3013 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3640 4420 4820 6132 6200 6661 9773 3640
Size: 780 400 1312 68 461 3112 3645
Fragments arranged by size:
3645 3112 1312 780 461 400 68
BlpI GC'TnA_GC
Cuts at: 7195 7195
Size: 9778
Bme1580I G_kGCm'C
Cuts at: 1790 2058 7839 8995 1790
Size: 268 5781 1156 2573
Fragments arranged by size:
5781 2573 1156 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5253 7007 7509
Size: 416 53 83 399 24 3836 1754 502
Cuts at: 7509 8467 442
Size: 958 1753
Fragments arranged by size:
3836 1754 1753 958 502 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5429 6774 1056
Size: 4373 1345 4060
Fragments arranged by size:
4373 4060 1345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2709 4664 5187 6121 6657 6855 7067
Size: 792 678 1955 523 934 536 198 212
Cuts at: 7067 8438 1239
Size: 1371 2579
Fragments arranged by size:
2579 1955 1371 934 792 678 536 523
212 198
Bpu10I CC'TnA_GC
Cuts at: 2946 4525 5157 6747 6995 2946
Size: 1579 632 1590 248 5729
Fragments arranged by size:
5729 1590 1579 632 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3377 7903 8768 9066 3377
Size: 4526 865 298 4089
Fragments arranged by size:
4526 4089 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5502 5780 6386 8441 882
Size: 617 4003 278 606 2055 2219
Fragments arranged by size:
4003 2219 2055 617 606 278
BsaAI yAC'GTr
Cuts at: 493 1204 3950 7569 493
Size: 711 2746 3619 2702
Fragments arranged by size:
3619 2746 2702 711
BsaBI GATnn'nnATC
Cuts at: 5978 9772 5978
Size: 3794 5984
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7585 7967 8602 275
Size: 53 83 186 936 6052 382 635 1451
Fragments arranged by size:
6052 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6896 8211 8609 8883 9030 9441 6896
Size: 1315 398 274 147 411 7233
Fragments arranged by size:
7233 1315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4637 4667 1178
Size: 30 165 30 3234 30 6289
Fragments arranged by size:
6289 3234 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2274 6565 1186
Size: 1088 4291 4399
Fragments arranged by size:
4399 4291 1088
BseYI C'CCAG_C
Cuts at: 5385 6925 7068 8981 9477 5385
Size: 1540 143 1913 496 5686
Fragments arranged by size:
5686 1913 1540 496 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 9205
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7228 7989 9017 1540
Size: 275 186 5227 761 1028 2301
Fragments arranged by size:
5227 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3966 4842 5194 5860 7055 7839 7924 8995 3966
Size: 876 352 666 1195 784 85 1071 4749
Fragments arranged by size:
4749 1195 1071 876 784 666 352 85
BsmI GAATG_Cn'
Cuts at: 2132 4855 6967 7289 7382 2132
Size: 2723 2112 322 93 4528
Fragments arranged by size:
4528 2723 2112 322 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 3966 4842 5194 5860 6887 7055
Size: 399 268 1908 876 352 666 1027 168
Cuts at: 7055 7097 7839 7924 8995 1391
Size: 42 742 85 1071 2174
Fragments arranged by size:
2174 1908 1071 1027 876 742 666 399
352 268 168 85 42
BspEI T'CCGG_A
Cuts at: 6896 9441 6896
Size: 2545 7233
BspHI T'CATG_A
Cuts at: 2886 5593 7667 9397 2886
Size: 2707 2074 1730 3267
Fragments arranged by size:
3267 2707 2074 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5881 7133 844
Size: 5037 1252 3489
Fragments arranged by size:
5037 3489 1252
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 3757 5514 6759 7665 1394
Size: 429 237 1697 1757 1245 906 3507
Fragments arranged by size:
3507 1757 1697 1245 906 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 8273 8447 65
Size: 1183 7025 174 1396
Fragments arranged by size:
7025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8422 8609 1700
Size: 110 6612 187 2869
Fragments arranged by size:
6612 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2596 5635 6273 96
Size: 2500 3039 638 3601
Fragments arranged by size:
3601 3039 2500 638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9778
BssSI C'ACGA_G
Cuts at: 4506 4581 7838 8850 4506
Size: 75 3257 1012 5434
Fragments arranged by size:
5434 3257 1012 75
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3865 6865 794
Size: 3071 3000 3707
Fragments arranged by size:
3707 3071 3000
BstBI TT'CG_AA
Cuts at: 2959 8582 2959
Size: 5623 4155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9778
BstXI CCAn_nnnn'nTGG
Cuts at: 3277 3277
Size: 9778
BstYI r'GATC_y
Cuts at: 1972 2950 3280 3640 4420 4820 5107 6132 6200
Size: 978 330 360 780 400 287 1025 68
Cuts at: 6200 6568 6651 6661 7108 7475 7860 7877 9329
Size: 368 83 10 447 367 385 17 1452
Cuts at: 9329 9415 9427 9773 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1025 978 780 447 400 385
368 367 360 346 330 287 86 83
68 17 12 10
BstZ17I GTA'TAC
Cuts at: 4388 4388
Size: 9778
Bsu36I CC'TnA_GG
Cuts at: 6684 6684
Size: 9778
BtgI C'CryG_G
Cuts at: 513 1065 2757 5851 6312 7060 513
Size: 552 1692 3094 461 748 3231
Fragments arranged by size:
3231 3094 1692 748 552 461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4506 487
Size: 683 56 3280 5759
Fragments arranged by size:
5759 3280 683 56
BtsI GCAGTG_nn'
Cuts at: 2081 6781 7294 8088 8108 2081
Size: 4700 513 794 20 3751
Fragments arranged by size:
4700 3751 794 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9743
DraI TTT'AAA
Cuts at: 3138 4221 5941 7082 3138
Size: 1083 1720 1141 5834
Fragments arranged by size:
5834 1720 1141 1083
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2932 5516 5742 8785 809
Size: 507 1616 2584 226 3043 1802
Fragments arranged by size:
3043 2584 1802 1616 507 226
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3184 3726 6224
Size: 54 1425 50 275 18 1354 542 2498
Cuts at: 6224 7225 8114 9743 8
Size: 1001 889 1629 43
Fragments arranged by size:
2498 1629 1425 1354 1001 889 542 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7225 1537
Size: 275 5413 4090
Fragments arranged by size:
5413 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 3813 4240 4404 4692 4806 6336 6525
Size: 790 1670 427 164 288 114 1530 189
Cuts at: 6525 7708 1353
Size: 1183 3423
Fragments arranged by size:
3423 1670 1530 1183 790 427 288 189
164 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8365 8739 8885 387
Size: 1064 138 6776 374 146 1280
Fragments arranged by size:
6776 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5858 5858
Size: 9778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2065 2547 2696 2709 3348 3945
Size: 393 399 34 482 149 13 639 597
Cuts at: 3945 4022 4344 4502 4563 4664 4830 5187 5259
Size: 77 322 158 61 101 166 357 72
Cuts at: 5259 5968 6121 6502 6657 6855 7067 7841 8438
Size: 709 153 381 155 198 212 774 597
Cuts at: 8438 9225 9493 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 774 709 639 597 597 482
399 393 381 357 322 268 212 198
166 158 155 153 149 101 77 72
61 34 13
EcoNI CCTnn'n_nnAGG
Cuts at: 5115 6750 5115
Size: 1635 8143
EcoO109I rG'GnC_Cy
Cuts at: 5093 5893 6632 7072 5093
Size: 800 739 440 7799
Fragments arranged by size:
7799 800 739 440
EcoRI G'AATT_C
Cuts at: 3737 6827 7087 7465 3737
Size: 3090 260 378 6050
Fragments arranged by size:
6050 3090 378 260
EcoRV GAT'ATC
Cuts at: 6295 6295
Size: 9778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3386 4783 5622 3386
Size: 1397 839 7542
Fragments arranged by size:
7542 1397 839
FspI TGC'GCA
Cuts at: 3869 6124 8284 3869
Size: 2255 2160 5363
Fragments arranged by size:
5363 2255 2160
HaeII r_GCGC'y
Cuts at: 1678 3355 8925 1678
Size: 1677 5570 2531
Fragments arranged by size:
5570 2531 1677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 2786 2818 4637
Size: 32 135 32 1148 32 34 32 1819
Cuts at: 4637 4669 4805 4837 5077 5109 6293 6313 6319
Size: 32 136 32 240 32 1184 20 6
Cuts at: 6319 6325 6345 6351 6941 6973 8419 8451 8493
Size: 6 20 6 590 32 1446 32 42
Cuts at: 8493 8525 1373
Size: 32 2626
Fragments arranged by size:
2626 1819 1446 1184 1148 590 240 136
135 42 34 32 32 32 32 32
32 32 32 32 32 20 20 6
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 4320 7122 7368 669
Size: 988 281 1461 921 2802 246 3079
Fragments arranged by size:
3079 2802 1461 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 3330 3850 4358 748
Size: 2582 520 508 6168
Fragments arranged by size:
6168 2582 520 508
HpaI GTT'AAC
Cuts at: 4320 7368 4320
Size: 3048 6730
KpnI G_GTAC'C
Cuts at: 7103 7103
Size: 9778
MfeI C'AATT_G
Cuts at: 2568 7377 2568
Size: 4809 4969
MluI A'CGCG_T
Cuts at: 9654 9654
Size: 9778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 5081 5327 6369 6452 6626 6674 7005
Size: 708 3068 246 1042 83 174 48 331
Cuts at: 7005 7014 8892 9076 1305
Size: 9 1878 184 2007
Fragments arranged by size:
3068 2007 1878 1042 708 331 246 184
174 83 48 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3186 3728 6226 10
Size: 54 1425 343 1354 542 2498 3562
Fragments arranged by size:
3562 2498 1425 1354 542 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4140 5869 6072 7736 8095 8254 518
Size: 648 2974 1729 203 1664 359 159 2042
Fragments arranged by size:
2974 2042 1729 1664 648 359 203 159
MspA1I CmG'CkG
Cuts at: 1606 4795 5165 7062 7194 7871 9019 9264 1606
Size: 3189 370 1897 132 677 1148 245 2120
Fragments arranged by size:
3189 2120 1897 1148 677 370 245 132
NcoI C'CATG_G
Cuts at: 513 1065 2757 5851 6312 513
Size: 552 1692 3094 461 3979
Fragments arranged by size:
3979 3094 1692 552 461
NdeI CA'TA_TG
Cuts at: 387 5134 387
Size: 4747 5031
NheI G'CTAG_C
Cuts at: 1052 5425 6770 1052
Size: 4373 1345 4060
Fragments arranged by size:
4373 4060 1345
NotI GC'GGCC_GC
Cuts at: 7225 7225
Size: 9778
NspI r_CATG'y
Cuts at: 1132 1229 2367 4139 5642 5868 7136 8681 1132
Size: 97 1138 1772 1503 226 1268 1545 2229
Fragments arranged by size:
2229 1772 1545 1503 1268 1138 226 97
PasI CC'CwG_GG
Cuts at: 1348 5098 1348
Size: 3750 6028
PciI A'CATG_T
Cuts at: 5638 8677 5638
Size: 3039 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3203 5974 6318 1108
Size: 2095 2771 344 4568
Fragments arranged by size:
4568 2771 2095 344
PfoI T'CCnGG_A
Cuts at: 2436 6634 2436
Size: 4198 5580
PmeI GTTT'AAAC
Cuts at: 7082 7082
Size: 9778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7847 7879 9360 9392 7847
Size: 32 1481 32 8233
Fragments arranged by size:
8233 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5093 5893 6632 7072 5093
Size: 800 739 440 7799
Fragments arranged by size:
7799 800 739 440
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9778
PsiI TTA'TAA
Cuts at: 7348 7348
Size: 9778
PspOMI G'GGCC_C
Cuts at: 2054 2054
Size: 9778
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6432 6464 6432
Size: 32 9746
PstI C_TGCA'G
Cuts at: 830 3423 7130 830
Size: 2593 3707 3478
Fragments arranged by size:
3707 3478 2593
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9778
PvuII CAG'CTG
Cuts at: 4795 5165 4795
Size: 370 9408
SacI G_AGCT'C
Cuts at: 5860 5860
Size: 9778
SacII CC_GC'GG
Cuts at: 7063 7063
Size: 9778
SalI G'TCGA_C
Cuts at: 1936 7120 1936
Size: 5184 4594
SanDI GG'GwC_CC
Cuts at: 7072 7072
Size: 9778
SapI GCTCTTCn'nnn_
Cuts at: 4806 4806
Size: 9778
SbfI CC_TGCA'GG
Cuts at: 7130 7130
Size: 9778
ScaI AGT'ACT
Cuts at: 8026 9738 8026
Size: 1712 8066
SexAI A'CCwGG_T
Cuts at: 2457 3828 2457
Size: 1371 8407
SfcI C'TryA_G
Cuts at: 826 1045 2280 2715 3419 5199 6844 7126 8261
Size: 219 1235 435 704 1780 1645 282 1135
Cuts at: 8261 8942 9133 826
Size: 681 191 1471
Fragments arranged by size:
1780 1645 1471 1235 1135 704 681 435
282 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9778
SmaI CCC'GGG
Cuts at: 1872 7105 1872
Size: 5233 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3356 3853 4157 4381 7882 8783
Size: 197 926 1413 497 304 224 3501 901
Cuts at: 8783 9045 820
Size: 262 1553
Fragments arranged by size:
3501 1553 1413 926 901 497 304 262
224 197
SnaBI TAC'GTA
Cuts at: 493 7569 493
Size: 7076 2702
SpeI A'CTAG_T
Cuts at: 152 3218 3655 152
Size: 3066 437 6275
Fragments arranged by size:
6275 3066 437
SphI G_CATG'C
Cuts at: 1132 7136 1132
Size: 6004 3774
SspI AAT'ATT
Cuts at: 5 52 2241 2325 3055 7702 5
Size: 47 2189 84 730 4647 2081
Fragments arranged by size:
4647 2189 2081 730 84 47
StuI AGG'CCT
Cuts at: 1517 4523 1517
Size: 3006 6772
StyI C'CwwG_G
Cuts at: 513 1065 2757 5851 6312 6910 7217 513
Size: 552 1692 3094 461 598 307 3074
Fragments arranged by size:
3094 3074 1692 598 552 461 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3039 3791 5446 5804 7805 7822 7975
Size: 1255 1459 752 1655 358 2001 17 153
Cuts at: 7975 8160 8605 325
Size: 185 445 1498
Fragments arranged by size:
2001 1655 1498 1459 1255 752 445 358
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2596 3229 5635
Size: 274 80 33 51 1443 619 633 2406
Cuts at: 5635 6273 8024 9736 96
Size: 638 1751 1712 138
Fragments arranged by size:
2406 1751 1712 1443 638 633 619 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3393 3425 3934 3966 6206 6238 9738 9770 3393
Size: 32 509 32 2240 32 3500 32 3401
Fragments arranged by size:
3500 3401 2240 509 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2991 1316
Size: 1675 8103
XbaI T'CTAG_A
Cuts at: 3307 6203 7114 3307
Size: 2896 911 5971
Fragments arranged by size:
5971 2896 911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3892 3892
Size: 9778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9778
XmaI C'CCGG_G
Cuts at: 1870 7103 1870
Size: 5233 4545
XmnI GAAnn'nnTTC
Cuts at: 2748 4476 5700 5910 6321 7907 2748
Size: 1728 1224 210 411 1586 4619
Fragments arranged by size:
4619 1728 1586 1224 411 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7586 276
Size: 53 83 186 936 6052 2468
Fragments arranged by size:
6052 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII
SfiI SfoI SrfI SwaI