Restriction map for
pFN21AB3393
(Circular) (Six-base) MAPSORT of: pFN21AB3393.seq Check: 5608 from: 1 to: 5629
pFN21AB3393 5629 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2572 2572
Size: 5629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2133 2950 2133
Size: 817 4812
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2894 3692 5076 5344 1632
Size: 569 693 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 693 569 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 2362 2793 4358 2362
Size: 431 1565 3633
Fragments arranged by size:
3633 1565 431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 2206 3372 4944 2206
Size: 1166 1572 2891
Fragments arranged by size:
2891 1572 1166
ApaI G_GGCC'C
Cuts at: 2254 2298 2254
Size: 44 5585
ApaLI G'TGCA_C
Cuts at: 2384 2447 3686 4842 2384
Size: 63 1239 1156 3171
Fragments arranged by size:
3171 1239 1156 63
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2216 2950 4405 618
Size: 325 217 627 346 83 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 346 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2079 2254 2298 2578 2948 1391
Size: 688 175 44 280 370 4072
Fragments arranged by size:
4072 688 370 280 175 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 928
Size: 971 367 4291
Fragments arranged by size:
4291 971 367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2622 4240 136
Size: 107 122 71 631 605 950 1618 1525
Fragments arranged by size:
1618 1525 950 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 2577 3046 2577
Size: 469 5160
Bme1580I G_kGCm'C
Cuts at: 1790 2219 2254 2298 2334 2388 2451 3690 4846
Size: 429 35 44 36 54 63 1239 1156
Cuts at: 4846 1790
Size: 2573
Fragments arranged by size:
2573 1239 1156 429 63 54 44 36
35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2518 2795 3360
Size: 416 53 83 399 24 1101 277 565
Cuts at: 3360 4318 442
Size: 958 1753
Fragments arranged by size:
1753 1101 958 565 416 399 277 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 4289 1239
Size: 1214 1836 2579
Fragments arranged by size:
2579 1836 1214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2715 3420 493
Size: 711 1511 705 2702
Fragments arranged by size:
2702 1511 711 705
BsaBI GATnn'nnATC
Cuts at: 2840 5623 2840
Size: 2783 2846
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 3818 4453 275
Size: 53 83 186 936 1903 382 635 1451
Fragments arranged by size:
1903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2441 2471 1178
Size: 30 165 30 636 30 372 30 4336
Fragments arranged by size:
4336 636 372 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5629
BseYI C'CCAG_C
Cuts at: 2262 2666 4832 5328 2262
Size: 404 2166 496 2563
Fragments arranged by size:
2563 2166 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2482 1163
Size: 573 746 4310
Fragments arranged by size:
4310 746 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2388 2451 2578 3690 3775 4846 2388
Size: 63 127 1112 85 1071 3171
Fragments arranged by size:
3171 1112 1071 127 85 63
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
BsmBI CGTCTCn'nnnn_
Cuts at: 2429 2429
Size: 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2219 2254 2298 2334 2388 2451
Size: 399 289 140 35 44 36 54 63
Cuts at: 2451 2578 2948 3690 3775 4846 1391
Size: 127 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 289 140 127
85 63 54 44 36 35
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 2784 3518 5248 2784
Size: 734 1730 3165
Fragments arranged by size:
3165 1730 734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2572 2664 2984 844
Size: 1475 253 92 320 3489
Fragments arranged by size:
3489 1475 320 253 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 4124 4298 65
Size: 1183 840 2036 174 1396
Fragments arranged by size:
2036 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 4217
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2641
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2685 2959 3326 3711 3728 5180 5266 5278
Size: 713 274 367 385 17 1452 86 12
Cuts at: 5278 5624 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 274 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2211 2220 2461 513
Size: 552 1146 9 241 3681
Fragments arranged by size:
3681 1146 552 241 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2135 2798 3145 3939 3959 2135
Size: 663 347 794 20 3805
Fragments arranged by size:
3805 794 663 347 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2344 3076
Size: 54 1425 50 275 18 393 121 732
Cuts at: 3076 3965 5594 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 732 393 275 121 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2321 2447 2726 3559 1353
Size: 761 207 126 279 833 3423
Fragments arranged by size:
3423 833 761 279 207 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 4216 4590 4736 387
Size: 1064 138 555 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 555 374 146 138
EcoICRI GAG'CTC
Cuts at: 2576 2576
Size: 5629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2453 2894 3692 4289 5076 5344
Size: 393 569 252 441 798 597 787 268
Cuts at: 5344 1239
Size: 1524
Fragments arranged by size:
1524 798 787 597 569 441 393 268
252
EcoO109I rG'GnC_Cy
Cuts at: 2242 2250 2294 2295 2653 2763 2242
Size: 8 44 1 358 110 5108
Fragments arranged by size:
5108 358 110 44 8 1
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2725 2125
Size: 600 5029
FspI TGC'GCA
Cuts at: 2480 2903 4135 2480
Size: 423 1232 3974
Fragments arranged by size:
3974 1232 423
HaeII r_GCGC'y
Cuts at: 1678 2428 2597 4776 1678
Size: 750 169 2179 2531
Fragments arranged by size:
2531 2179 750 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2747 2779 2811
Size: 32 135 32 776 32 367 32 32
Cuts at: 2811 4270 4302 4344 4376 1373
Size: 1459 32 42 32 2626
Fragments arranged by size:
2626 1459 776 367 135 42 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2137 2954 2137
Size: 817 4812
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 10
Size: 54 1425 343 514 3293
Fragments arranged by size:
3293 1425 514 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 2639 2834 3587 3946 4105 518
Size: 648 1186 287 195 753 359 159 2042
Fragments arranged by size:
2042 1186 753 648 359 287 195 159
MspA1I CmG'CkG
Cuts at: 1606 2262 2666 3045 3722 4870 5115 1606
Size: 656 404 379 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 656 404 379 245
NcoI C'CATG_G
Cuts at: 513 1065 2461 513
Size: 552 1396 3681
Fragments arranged by size:
3681 1396 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2908 2987 4532 1132
Size: 97 1679 79 1545 2229
Fragments arranged by size:
2229 1679 1545 97 79
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PfoI T'CCnGG_A
Cuts at: 2238 2765 2883 2238
Size: 527 118 4984
Fragments arranged by size:
4984 527 118
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PmlI CAC'GTG
Cuts at: 2715 2715
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2653 2763 2242
Size: 411 110 5108
Fragments arranged by size:
5108 411 110
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2250 2294 2250
Size: 44 5585
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 5597
PstI C_TGCA'G
Cuts at: 830 2202 2981 830
Size: 1372 779 3478
Fragments arranged by size:
3478 1372 779
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2262 2262
Size: 5629
SacI G_AGCT'C
Cuts at: 2578 2578
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SapI GCTCTTCn'nnn_
Cuts at: 2321 2447 2726 2321
Size: 126 279 5224
Fragments arranged by size:
5224 279 126
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2198 2977 4112 4793 4984 826
Size: 219 1153 779 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 779 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2908 2987 1132
Size: 1776 79 3774
Fragments arranged by size:
3774 1776 79
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 2166 2461 2850 3068 513
Size: 552 1101 295 389 218 3074
Fragments arranged by size:
3074 1101 552 389 295 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 2846 3656 3673 3826 4011 4456
Size: 1255 674 592 810 17 153 185 445
Cuts at: 4456 325
Size: 1498
Fragments arranged by size:
1498 1255 810 674 592 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 3875 5587
Size: 274 80 33 51 1443 255 1643 1712
Cuts at: 5587 96
Size: 138
Fragments arranged by size:
1712 1643 1443 274 255 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 2220 2252 2441 2473 5589 5621 2039
Size: 32 149 32 189 32 3116 32 2047
Fragments arranged by size:
3116 2047 189 149 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 2108 2708 2879 3758 2108
Size: 600 171 879 3979
Fragments arranged by size:
3979 879 600 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI