Restriction map for
pFN21AB3392
(Circular) (Six-base) MAPSORT of: pFN21AB3392.seq Check: 5869 from: 1 to: 5743
pFN21AB3392 5743 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2469 3086 1937
Size: 532 617 4594
Fragments arranged by size:
4594 617 532
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3042 3870 4243 1563
Size: 1479 828 373 3063
Fragments arranged by size:
3063 1479 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 2504 3806 5190 5458 1632
Size: 680 192 1302 1384 268 1917
Fragments arranged by size:
1917 1384 1302 680 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 2661 4642 5619 1203
Size: 1458 1981 977 1327
Fragments arranged by size:
1981 1458 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2376 1184
Size: 1192 4551
AlwNI CAG_nnn'CTG
Cuts at: 3486 5058 3486
Size: 1572 4171
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2021 3052 3278 3430 5539 1493
Size: 528 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 528 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 3068 1870
Size: 73 1125 4545
Fragments arranged by size:
4545 1125 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2117 3062 1391
Size: 726 945 4072
Fragments arranged by size:
4072 945 726
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 928
Size: 971 699 4073
Fragments arranged by size:
4073 971 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 2892 1548
Size: 1344 4399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2491 5738 2491
Size: 3247 2496
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 3804 4960 1790
Size: 2014 1156 2573
Fragments arranged by size:
2573 2014 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2331 2544 2786 4403 1239
Size: 1004 88 213 242 1617 2579
Fragments arranged by size:
2579 1617 1004 242 213 88
Bpu10I CC'TnA_GC
Cuts at: 2556 2556
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2336 3868 4733 5031 2012
Size: 324 1532 865 298 2724
Fragments arranged by size:
2724 1532 865 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3027 3057 1178
Size: 30 165 30 1624 30 3864
Fragments arranged by size:
3864 1624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4596
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3254 3347 2942
Size: 312 93 5338
Fragments arranged by size:
5338 312 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 3062 3804 3889 4960 1391
Size: 399 327 945 742 85 1071 2174
Fragments arranged by size:
2174 1071 945 742 399 327 85
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 4238 4412 65
Size: 1183 768 2222 174 1396
Fragments arranged by size:
2222 1396 1183 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 2309 2561 1184
Size: 1125 252 4366
Fragments arranged by size:
4366 1125 252
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2491 3073 3440 3825 3842 5294 5380 5392
Size: 519 582 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 582 519 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 3190 4079
Size: 54 1425 50 275 18 666 694 889
Cuts at: 4079 5708 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 694 666 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2480 3673 1353
Size: 696 431 1193 3423
Fragments arranged by size:
3423 1193 696 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2312 2331 2504 2544 2786 3806
Size: 393 611 69 19 173 40 242 1020
Cuts at: 3806 4403 5190 5458 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1020 787 611 597 393 268 242
173 69 40 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2318 2030
Size: 288 5455
EcoO109I rG'GnC_Cy
Cuts at: 2389 2616 2389
Size: 227 5516
EcoRI G'AATT_C
Cuts at: 2021 3052 3430 2021
Size: 1031 378 4334
Fragments arranged by size:
4334 1031 378
EcoRV GAT'ATC
Cuts at: 2935 2935
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 2495 2527 4384
Size: 32 135 32 532 32 359 32 1857
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1857 532 359 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2237 3087 3333 669
Size: 988 281 299 850 246 3079
Fragments arranged by size:
3079 988 850 299 281 246
HindIII A'AGCT_T
Cuts at: 748 2846 748
Size: 2098 3645
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2851 4857 5041 1305
Size: 1546 2006 184 2007
Fragments arranged by size:
2007 2006 1546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3255
Fragments arranged by size:
3255 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3701 4060 4219 518
Size: 648 843 1692 359 159 2042
Fragments arranged by size:
2042 1692 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 3159 3836 4984 5229 1606
Size: 502 1051 677 1148 245 2120
Fragments arranged by size:
2120 1148 1051 677 502 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PfoI T'CCnGG_A
Cuts at: 2098 2259 2985 2098
Size: 161 726 4856
Fragments arranged by size:
4856 726 161
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 3095 830
Size: 2265 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2049 2049
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2470 3091 4226 4907 5098 826
Size: 219 1425 621 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 681 621 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 2315 3847 4748 5010 820
Size: 197 926 84 288 1532 901 262 1553
Fragments arranged by size:
1553 1532 926 901 288 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2036 1517
Size: 519 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2392 3182 513
Size: 552 939 388 790 3074
Fragments arranged by size:
3074 939 790 552 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2580 3770 3787 3940 4125 4570 325
Size: 1255 1000 1190 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1190 1000 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3989 5701
Size: 274 80 33 51 1443 753 1259 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1443 1259 753 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2094 2126 5703 5735 2094
Size: 32 3577 32 2102
Fragments arranged by size:
3577 2102 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 2067 3872 2067
Size: 1805 3938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI