Restriction map for
pFN21AB3389
(Circular) (Six-base) MAPSORT of: pFN21AB3389.seq Check: 4976 from: 1 to: 6061
pFN21AB3389 6061 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2397 3044 2397
Size: 647 5414
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3069 3404 1937
Size: 1132 335 4594
Fragments arranged by size:
4594 1132 335
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3180 4124 5508 5776 1632
Size: 1548 944 1384 268 1917
Fragments arranged by size:
1917 1548 1384 944 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2081 3112 3804 5376 2081
Size: 1031 692 1572 2766
Fragments arranged by size:
2766 1572 1031 692
ApaI G_GGCC'C
Cuts at: 2371 2371
Size: 6061
ApaLI G'TGCA_C
Cuts at: 2586 4118 5274 2586
Size: 1532 1156 3373
Fragments arranged by size:
3373 1532 1156
ApoI r'AATT_y
Cuts at: 1493 2044 2505 2630 3047 3140 3370 3596 3748
Size: 551 461 125 417 93 230 226 152
Cuts at: 3748 5857 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 551 461 417 230 226 152
125 93
AseI AT'TA_AT
Cuts at: 160 1032 2820 4615 160
Size: 872 1788 1795 1606
Fragments arranged by size:
1795 1788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 3386 1870
Size: 73 234 1209 4545
Fragments arranged by size:
4545 1209 234 73
BamHI G'GATC_C
Cuts at: 2189 2653 2742 3391 3758 2189
Size: 464 89 649 367 4492
Fragments arranged by size:
4492 649 464 367 89
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2206 2311 3382 4837
Size: 325 217 627 385 34 105 1071 1455
Cuts at: 4837 618
Size: 1842
Fragments arranged by size:
1842 1455 1071 627 385 325 217 105
34
BanII G_rGCy'C
Cuts at: 1391 2070 2371 2909 3380 1391
Size: 679 301 538 471 4072
Fragments arranged by size:
4072 679 538 471 301
BbeI G_GCGC'C
Cuts at: 2176 2210 2176
Size: 34 6027
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2820 928
Size: 971 921 4169
Fragments arranged by size:
4169 971 921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4252 4286 1903
Size: 34 50 34 2231 34 3678
Fragments arranged by size:
3678 2231 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2999 3093 3953 5163 2999
Size: 94 860 1210 3897
Fragments arranged by size:
3897 1210 860 94
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2371 2590 4122 5278 1790
Size: 526 55 219 1532 1156 2573
Fragments arranged by size:
2573 1532 1156 526 219 55
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 3792 4750
Size: 416 53 83 399 24 993 1382 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1382 993 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2846 4721 1239
Size: 859 748 1875 2579
Fragments arranged by size:
2579 1875 859 748
Bpu10I CC'TnA_GC
Cuts at: 2372 2372
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2470 3852 493
Size: 711 1266 1382 2702
Fragments arranged by size:
2702 1382 1266 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2173 2207 3868 4250
Size: 53 83 186 936 640 34 1661 382
Cuts at: 4250 4885 275
Size: 635 1451
Fragments arranged by size:
1661 1451 936 640 635 382 186 83
53 34
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 1186
Size: 1174 4887
BseYI C'CCAG_C
Cuts at: 2146 2155 2674 5264 5760 2146
Size: 9 519 2590 496 2447
Fragments arranged by size:
2590 2447 519 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2539 3511 4272 5300 1540
Size: 275 186 16 522 972 761 1028 2301
Fragments arranged by size:
2301 1028 972 761 522 275 186 16
BsiHKAI G_wGCw'C
Cuts at: 2590 4122 4207 5278 2590
Size: 1532 85 1071 3373
Fragments arranged by size:
3373 1532 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2316 2371 2590 2909 3380 4122
Size: 399 280 246 55 219 319 471 742
Cuts at: 4122 4207 5278 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 319 280 246
219 85 55
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 3044 3416 844
Size: 1553 647 372 3489
Fragments arranged by size:
3489 1553 647 372
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2132 3948 1394
Size: 429 236 73 1816 3507
Fragments arranged by size:
3507 1816 429 236 73
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2977 96
Size: 2881 3180
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 2219 4121 5133 2219
Size: 1902 1012 3147
Fragments arranged by size:
3147 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 794
Size: 1595 4466
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2416 1184
Size: 1232 4829
BstYI r'GATC_y
Cuts at: 1972 2189 2653 2742 3391 3758 4143 4160 5612
Size: 217 464 89 649 367 385 17 1452
Cuts at: 5612 5698 5710 6056 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 649 464 385 367 346 217
89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3333 3357 3333
Size: 24 6037
BtgI C'CryG_G
Cuts at: 513 1065 2021 2271 2481 513
Size: 552 956 250 210 4093
Fragments arranged by size:
4093 956 552 250 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2336 487
Size: 683 56 1110 4212
Fragments arranged by size:
4212 1110 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2785 2820 529
Size: 35 2221 35 3770
Fragments arranged by size:
3770 2221 35 35
DraI TTT'AAA
Cuts at: 3322 3365 3322
Size: 43 6018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2274 3508
Size: 54 1425 50 275 18 184 260 1234
Cuts at: 3508 4397 6026 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1234 889 275 260 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2014 3508 1537
Size: 275 202 1494 4090
Fragments arranged by size:
4090 1494 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 2862 3194 3991 1353
Size: 1082 427 332 797 3423
Fragments arranged by size:
3423 1082 797 427 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2245 4648 5022 5168 387
Size: 1064 138 656 2403 374 146 1280
Fragments arranged by size:
2403 1280 1064 656 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2846 3180 4124 4721 5508 5776
Size: 393 466 748 334 944 597 787 268
Cuts at: 5776 1239
Size: 1524
Fragments arranged by size:
1524 944 787 748 597 466 393 334
268
EcoO109I rG'GnC_Cy
Cuts at: 2367 2368 2404 2367
Size: 1 36 6024
Fragments arranged by size:
6024 36 1
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
EcoRV GAT'ATC
Cuts at: 2842 2842
Size: 6061
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2153 2176 2210 5208 1678
Size: 475 23 34 2998 2531
Fragments arranged by size:
2998 2531 475 34 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373
Size: 32 135 32 3130 32 42 32 2626
Fragments arranged by size:
3130 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2172 2206 2172
Size: 34 6027
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3297 3660 3297
Size: 363 5698
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 2922 5175 5359 1305
Size: 753 864 2253 184 2007
Fragments arranged by size:
2253 2007 864 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 10
Size: 54 1425 343 444 3795
Fragments arranged by size:
3795 1425 444 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 3042 4019 4378 4537 518
Size: 648 1417 459 977 359 159 2042
Fragments arranged by size:
2042 1417 977 648 459 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2078 3477 4154 5302 5547 1606
Size: 417 55 1399 677 1148 245 2120
Fragments arranged by size:
2120 1399 1148 677 417 245 55
NarI GG'CG_CC
Cuts at: 2173 2207 2173
Size: 34 6027
NcoI C'CATG_G
Cuts at: 513 1065 2271 2481 513
Size: 552 1206 210 4093
Fragments arranged by size:
4093 1206 552 210
NdeI CA'TA_TG
Cuts at: 387 3022 387
Size: 2635 3426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 2014 3508 2014
Size: 1494 4567
NspI r_CATG'y
Cuts at: 1132 1229 2666 3419 4964 1132
Size: 97 1437 753 1545 2229
Fragments arranged by size:
2229 1545 1437 753 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PfoI T'CCnGG_A
Cuts at: 2862 2862
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2404
Size: 6061
PshAI GACnn'nnGTC
Cuts at: 1613 3075 1613
Size: 1462 4599
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2367 2367
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2427 3413 830
Size: 1597 986 3478
Fragments arranged by size:
3478 1597 986
PvuI CG_AT'CG
Cuts at: 2001 2539 2001
Size: 538 5523
PvuII CAG'CTG
Cuts at: 2078 2078
Size: 6061
SacII CC_GC'GG
Cuts at: 2024 2024
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SanDI GG'GwC_CC
Cuts at: 2404 2404
Size: 6061
SapI GCTCTTCn'nnn_
Cuts at: 2435 2435
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 3317 4309 6021 3317
Size: 992 1712 3357
Fragments arranged by size:
3357 1712 992
SfcI C'TryA_G
Cuts at: 826 1045 2423 3073 3098 3409 4544 5225 5416
Size: 219 1378 650 25 311 1135 681 191
Cuts at: 5416 826
Size: 1471
Fragments arranged by size:
1471 1378 1135 681 650 311 219 191
25
SfoI GGC'GCC
Cuts at: 2174 2208 2174
Size: 34 6027
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 2179 3388 1872
Size: 307 1209 4545
Fragments arranged by size:
4545 1209 307
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 2999 3985 5
Size: 47 2947 986 2081
Fragments arranged by size:
2947 2081 986 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2271 2481 3286 3500 513
Size: 552 1206 210 805 214 3074
Fragments arranged by size:
3074 1206 805 552 214 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2571 4088 4105 4258 4443 4888 325
Size: 1255 991 1517 17 153 185 445 1498
Fragments arranged by size:
1517 1498 1255 991 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2977 3315 4307
Size: 274 80 33 51 1443 1000 338 992
Cuts at: 4307 6019 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1000 992 338 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 2177 3386 1870
Size: 307 1209 4545
Fragments arranged by size:
4545 1209 307
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI
XcmI