Restriction map for
pFN21AB3385
(Circular) (Six-base) MAPSORT of: pFN21AB3385.seq Check: 7757 from: 1 to: 5269
pFN21AB3385 5269 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3079 278
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2612 1937
Size: 675 4594
Acc65I G'GTAC_C
Cuts at: 2287 2590 2287
Size: 303 4966
AclI AA'CG_TT
Cuts at: 1563 2568 3396 3769 1563
Size: 1005 828 373 3063
Fragments arranged by size:
3063 1005 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 3332 4716 4984 1632
Size: 686 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1014 686 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5072
AflIII A'CryG_T
Cuts at: 1203 4168 5145 1203
Size: 2965 977 1327
Fragments arranged by size:
2965 1327 977
AgeI A'CCGG_T
Cuts at: 4100 4100
Size: 5269
AhdI GACnn_n'nnGTC
Cuts at: 3998 3998
Size: 5269
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 4021
AlwNI CAG_nnn'CTG
Cuts at: 3012 4584 3012
Size: 1572 3697
ApaI G_GGCC'C
Cuts at: 2332 2332
Size: 5269
ApaLI G'TGCA_C
Cuts at: 2307 3326 4482 2307
Size: 1019 1156 3094
Fragments arranged by size:
3094 1156 1019
ApoI r'AATT_y
Cuts at: 1493 2578 2804 2956 5065 1493
Size: 1085 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3823 160
Size: 872 2791 1606
Fragments arranged by size:
2791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
AvaI C'yCGr_G
Cuts at: 1870 1943 2594 1870
Size: 73 651 4545
Fragments arranged by size:
4545 651 73
BamHI G'GATC_C
Cuts at: 2599 2966 2599
Size: 367 4902
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2590 4045 618
Size: 325 217 627 500 303 1455 1842
Fragments arranged by size:
1842 1455 627 500 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2058 2332 2588 1391
Size: 667 274 256 4072
Fragments arranged by size:
4072 667 274 256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3460 3494 1903
Size: 34 1523 34 3678
Fragments arranged by size:
3678 1523 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 4371 3161
Size: 1210 4059
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3880 136
Size: 107 122 71 631 605 2208 1525
Fragments arranged by size:
2208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5264 5264
Size: 5269
BlpI GC'TnA_GC
Cuts at: 2686 2686
Size: 5269
Bme1580I G_kGCm'C
Cuts at: 1790 2311 2332 3330 4486 1790
Size: 521 21 998 1156 2573
Fragments arranged by size:
2573 1156 998 521 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3000 3958 442
Size: 416 53 83 399 24 1583 958 1753
Fragments arranged by size:
1753 1583 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3929 1239
Size: 2690 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 4259 4557 3394
Size: 865 298 4106
Fragments arranged by size:
4106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3932 882
Size: 617 2433 2219
Fragments arranged by size:
2433 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3060 493
Size: 711 1856 2702
Fragments arranged by size:
2702 1856 711
BsaBI GATnn'nnATC
Cuts at: 5263 5263
Size: 5269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3076 3458 4093 275
Size: 53 83 186 936 1543 382 635 1451
Fragments arranged by size:
1543 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2205 2423 3702 4100 4374 4521 4932 2205
Size: 218 1279 398 274 147 411 2542
Fragments arranged by size:
2542 1279 411 398 274 218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 3925
Fragments arranged by size:
3925 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5269
BseYI C'CCAG_C
Cuts at: 4472 4968 4472
Size: 496 4773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4696
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2180 2244 2719 3480 4508 1540
Size: 275 186 179 64 475 761 1028 2301
Fragments arranged by size:
2301 1028 761 475 275 186 179 64
BsiHKAI G_wGCw'C
Cuts at: 2311 3330 3415 4486 2311
Size: 1019 85 1071 3094
Fragments arranged by size:
3094 1071 1019 85
BsmI GAATG_Cn'
Cuts at: 2780 2873 2780
Size: 93 5176
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2311 2332 2588 3330 3415 4486
Size: 399 268 253 21 256 742 85 1071
Cuts at: 4486 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 268 256 253 85
21
BspEI T'CCGG_A
Cuts at: 2205 4932 2205
Size: 2727 2542
BspHI T'CATG_A
Cuts at: 3158 4888 3158
Size: 1730 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2624 844
Size: 1780 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2508 3156 1394
Size: 429 685 648 3507
Fragments arranged by size:
3507 685 648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3764 3938 65
Size: 1183 2516 174 1396
Fragments arranged by size:
2516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 2451 3913 4100 1700
Size: 110 365 276 1462 187 2869
Fragments arranged by size:
2869 1462 365 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5269
BssSI C'ACGA_G
Cuts at: 3329 4341 3329
Size: 1012 4257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5269
BstBI TT'CG_AA
Cuts at: 4073 4073
Size: 5269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5269
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5269
BstYI r'GATC_y
Cuts at: 1972 2361 2599 2966 3351 3368 4820 4906 4918
Size: 389 238 367 385 17 1452 86 12
Cuts at: 4918 5264 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 389 385 367 346 238 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2078 2345 2397 2078
Size: 267 52 4950
Fragments arranged by size:
4950 267 52
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 513
Size: 552 939 240 3538
Fragments arranged by size:
3538 939 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4530
Fragments arranged by size:
4530 683 56
BtsI GCAGTG_nn'
Cuts at: 2309 2785 3579 3599 2309
Size: 476 794 20 3979
Fragments arranged by size:
3979 794 476 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5234
DraI TTT'AAA
Cuts at: 2573 2573
Size: 5269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4276 809
Size: 507 2960 1802
Fragments arranged by size:
2960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2177 2224
Size: 54 1425 50 275 18 279 68 47
Cuts at: 2224 2241 2716 3605 5234 8
Size: 17 475 889 1629 43
Fragments arranged by size:
1629 1425 889 475 279 275 68 54
50 47 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2177 2241 2716 1537
Size: 275 365 64 475 4090
Fragments arranged by size:
4090 475 365 275 64
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3199 1353
Size: 791 1055 3423
Fragments arranged by size:
3423 1055 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2009 3856 4230 4376 387
Size: 1064 138 420 1847 374 146 1280
Fragments arranged by size:
1847 1280 1064 420 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 3332 3929 4716 4984 1239
Size: 393 686 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 787 686 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2467 2467
Size: 5269
EcoRI G'AATT_C
Cuts at: 2578 2956 2578
Size: 378 4891
EcoRV GAT'ATC
Cuts at: 2071 2071
Size: 5269
FspI TGC'GCA
Cuts at: 3775 3775
Size: 5269
HaeII r_GCGC'y
Cuts at: 1678 4416 1678
Size: 2738 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2001 2033 3910 3942 3984
Size: 32 135 32 429 32 1877 32 42
Cuts at: 3984 4016 1373
Size: 32 2626
Fragments arranged by size:
2626 1877 429 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2613 2859 669
Size: 988 281 675 246 3079
Fragments arranged by size:
3079 988 675 281 246
HindIII A'AGCT_T
Cuts at: 748 2216 748
Size: 1468 3801
HpaI GTT'AAC
Cuts at: 2859 2859
Size: 5269
KpnI G_GTAC'C
Cuts at: 2291 2594 2291
Size: 303 4966
MfeI C'AATT_G
Cuts at: 2868 2868
Size: 5269
MluI A'CGCG_T
Cuts at: 5145 5145
Size: 5269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4383 4567 1305
Size: 3078 184 2007
Fragments arranged by size:
3078 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 10
Size: 54 1425 343 279 3168
Fragments arranged by size:
3168 1425 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3227 3586 3745 518
Size: 648 843 1218 359 159 2042
Fragments arranged by size:
2042 1218 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2380 2685 3362 4510 4755 1606
Size: 774 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 677 305 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3778
Fragments arranged by size:
3778 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5269
NotI GC'GGCC_GC
Cuts at: 2716 2716
Size: 5269
NspI r_CATG'y
Cuts at: 1132 1229 2627 4172 1132
Size: 97 1398 1545 2229
Fragments arranged by size:
2229 1545 1398 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5269
PciI A'CATG_T
Cuts at: 4168 4168
Size: 5269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5269
PfoI T'CCnGG_A
Cuts at: 2195 2195
Size: 5269
PmeI GTTT'AAAC
Cuts at: 2573 2573
Size: 5269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 4851 4883 3338
Size: 32 1481 32 3724
Fragments arranged by size:
3724 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5269
PsiI TTA'TAA
Cuts at: 2839 2839
Size: 5269
PspOMI G'GGCC_C
Cuts at: 2328 2328
Size: 5269
PstI C_TGCA'G
Cuts at: 830 2621 830
Size: 1791 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5269
PvuII CAG'CTG
Cuts at: 2380 2380
Size: 5269
SalI G'TCGA_C
Cuts at: 1936 2611 1936
Size: 675 4594
SbfI CC_TGCA'GG
Cuts at: 2621 2621
Size: 5269
ScaI AGT'ACT
Cuts at: 3517 5229 3517
Size: 1712 3557
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 5269
SfcI C'TryA_G
Cuts at: 826 1045 2617 3752 4433 4624 826
Size: 219 1572 1135 681 191 1471
Fragments arranged by size:
1572 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5269
SmaI CCC'GGG
Cuts at: 1872 2596 1872
Size: 724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3373 4274 4536 820
Size: 197 926 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3060 493
Size: 2567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5269
SphI G_CATG'C
Cuts at: 1132 2627 1132
Size: 1495 3774
SspI AAT'ATT
Cuts at: 5 52 3193 5
Size: 47 3141 2081
Fragments arranged by size:
3141 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2511 2708 513
Size: 552 939 507 197 3074
Fragments arranged by size:
3074 939 552 507 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 3296 3313 3466 3651 4096 325
Size: 1255 469 1247 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1247 469 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3515 5227 96
Size: 274 80 33 51 1443 1538 1712 138
Fragments arranged by size:
1712 1538 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5229 5261 5229
Size: 32 5237
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5269
XbaI T'CTAG_A
Cuts at: 2605 2605
Size: 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5269
XmaI C'CCGG_G
Cuts at: 1870 2594 1870
Size: 724 4545
XmnI GAAnn'nnTTC
Cuts at: 3398 3398
Size: 5269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3077 276
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI