Restriction map for
pFN21AB3384
(Circular) (Six-base) MAPSORT of: pFN21AB3384.seq Check: 3186 from: 1 to: 6802
pFN21AB3384 6802 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2753 2753
Size: 6802
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4612 278
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4145 1937
Size: 2208 4594
Acc65I G'GTAC_C
Cuts at: 4123 4123
Size: 6802
AclI AA'CG_TT
Cuts at: 1563 4929 5302 1563
Size: 3366 373 3063
Fragments arranged by size:
3366 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2763 3965 4865 6249 6517 1632
Size: 1131 1202 900 1384 268 1917
Fragments arranged by size:
1917 1384 1202 1131 900 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6605
AflIII A'CryG_T
Cuts at: 1203 2518 3677 5701 6678 1203
Size: 1315 1159 2024 977 1327
Fragments arranged by size:
2024 1327 1315 1159 977
AgeI A'CCGG_T
Cuts at: 5633 5633
Size: 6802
AhdI GACnn_n'nnGTC
Cuts at: 5531 5531
Size: 6802
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6802
AlwNI CAG_nnn'CTG
Cuts at: 2786 3050 3229 4545 6117 2786
Size: 264 179 1316 1572 3471
Fragments arranged by size:
3471 1572 1316 264 179
ApaLI G'TGCA_C
Cuts at: 4859 6015 4859
Size: 1156 5646
ApoI r'AATT_y
Cuts at: 1493 3369 3902 4096 4111 4337 4489 6598 1493
Size: 1876 533 194 15 226 152 2109 1697
Fragments arranged by size:
2109 1876 1697 533 226 194 152 15
AseI AT'TA_AT
Cuts at: 160 1032 5356 160
Size: 872 4324 1606
Fragments arranged by size:
4324 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 3555 3897 4127 1870
Size: 73 1431 181 342 230 4545
Fragments arranged by size:
4545 1431 342 230 181 73
BamHI G'GATC_C
Cuts at: 3416 3586 4132 4499 3416
Size: 170 546 367 5719
Fragments arranged by size:
5719 546 367 170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4123 5578 618
Size: 325 217 627 2336 1455 1842
Fragments arranged by size:
2336 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 4121 1391
Size: 621 2109 4072
Fragments arranged by size:
4072 2109 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2524 3261 3672 3813 3831 928
Size: 971 625 737 411 141 18 3899
Fragments arranged by size:
3899 971 737 625 411 141 18
BbvCI CC'TCA_GC
Cuts at: 2739 2959 3039 3072 4063 2739
Size: 220 80 33 991 5478
Fragments arranged by size:
5478 991 220 80 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2515 2549 4993 5027 1903
Size: 34 578 34 2444 34 3678
Fragments arranged by size:
3678 2444 578 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2508 4694 5904 2508
Size: 2186 1210 3406
Fragments arranged by size:
3406 2186 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6802
BfrBI ATG'CAT
Cuts at: 2717 2717
Size: 6802
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 5413 136
Size: 107 122 71 631 605 361 3380 1525
Fragments arranged by size:
3380 1525 631 605 361 122 107 71
BglII A'GATC_T
Cuts at: 6797 6797
Size: 6802
BlpI GC'TnA_GC
Cuts at: 4219 4219
Size: 6802
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2383 4863 6019 1790
Size: 520 73 2480 1156 2573
Fragments arranged by size:
2573 2480 1156 520 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 2588 3540
Size: 416 53 83 399 24 1025 146 952
Cuts at: 3540 4533 5491 442
Size: 993 958 1753
Fragments arranged by size:
1753 1025 993 958 952 416 399 146
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2817 2817
Size: 6802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3239 3508 3760 3973 5462 1239
Size: 2000 269 252 213 1489 2579
Fragments arranged by size:
2579 2000 1489 269 252 213
Bpu10I CC'TnA_GC
Cuts at: 2739 2959 3039 3072 4063 2739
Size: 220 80 33 991 5478
Fragments arranged by size:
5478 991 220 80 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 4927 5792 6090 2543
Size: 2384 865 298 3255
Fragments arranged by size:
3255 2384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 5465 882
Size: 617 754 3212 2219
Fragments arranged by size:
3212 2219 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 4593 493
Size: 711 1961 1428 2702
Fragments arranged by size:
2702 1961 1428 711
BsaBI GATnn'nnATC
Cuts at: 3135 6796 3135
Size: 3661 3141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4609 4991 5626 275
Size: 53 83 186 936 3076 382 635 1451
Fragments arranged by size:
3076 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5235 5633 5907 6054 6465 5235
Size: 398 274 147 411 5572
Fragments arranged by size:
5572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6577
Fragments arranged by size:
6577 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 2103 2189 2192 2216 2546 2760 2763
Size: 914 3 86 3 24 330 214 3
Cuts at: 2763 3044 3494 3933 1186
Size: 281 450 439 4055
Fragments arranged by size:
4055 914 450 439 330 281 214 86
24 3 3 3
BseYI C'CCAG_C
Cuts at: 2331 2382 6005 6501 2331
Size: 51 3623 496 2632
Fragments arranged by size:
3623 2632 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 3990 1163
Size: 573 1213 1041 3975
Fragments arranged by size:
3975 1213 1041 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4252 5013 6041 1540
Size: 275 186 2251 761 1028 2301
Fragments arranged by size:
2301 2251 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2770 3746 4863 4948 6019 2012
Size: 758 976 1117 85 1071 2795
Fragments arranged by size:
2795 1117 1071 976 758 85
BsmI GAATG_Cn'
Cuts at: 3601 4079 4313 4406 3601
Size: 478 234 93 5997
Fragments arranged by size:
5997 478 234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3813 3813
Size: 6802
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2310 2383 2770 3746 4121 4863
Size: 399 222 298 73 387 976 375 742
Cuts at: 4863 4948 6019 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 976 742 399 387 375 298
222 85 73
BspEI T'CCGG_A
Cuts at: 6465 6465
Size: 6802
BspHI T'CATG_A
Cuts at: 4691 6421 4691
Size: 1730 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2753 4157 844
Size: 1909 1404 3489
Fragments arranged by size:
3489 1909 1404
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 3713 4689 1394
Size: 429 1867 23 976 3507
Fragments arranged by size:
3507 1867 976 429 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 5297 5471 65
Size: 1183 1119 2930 174 1396
Fragments arranged by size:
2930 1396 1183 1119 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2274 5446 5633 1700
Size: 110 464 3172 187 2869
Fragments arranged by size:
3172 2869 464 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6802
BssSI C'ACGA_G
Cuts at: 4862 5874 4862
Size: 1012 5790
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3050 794
Size: 2256 4546
BstBI TT'CG_AA
Cuts at: 2535 5606 2535
Size: 3071 3731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6802
BstYI r'GATC_y
Cuts at: 1972 2181 3416 3586 4132 4499 4884 4901 6353
Size: 209 1235 170 546 367 385 17 1452
Cuts at: 6353 6439 6451 6797 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1235 546 385 367 346 209
170 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3270 3270
Size: 6802
BtgI C'CryG_G
Cuts at: 513 1065 3590 513
Size: 552 2525 3725
Fragments arranged by size:
3725 2525 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6063
Fragments arranged by size:
6063 683 56
BtsI GCAGTG_nn'
Cuts at: 2796 4318 5112 5132 2796
Size: 1522 794 20 4466
Fragments arranged by size:
4466 1522 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6767
DraI TTT'AAA
Cuts at: 4106 4106
Size: 6802
DraIII CAC_nnn'GTG
Cuts at: 2751 3750 2751
Size: 999 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 5809 809
Size: 507 1155 3338 1802
Fragments arranged by size:
3338 1802 1155 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2272 2545
Size: 54 1425 50 275 18 195 247 273
Cuts at: 2545 3190 4249 5138 6767 8
Size: 645 1059 889 1629 43
Fragments arranged by size:
1629 1425 1059 889 645 275 273 247
195 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4249 1537
Size: 275 2437 4090
Fragments arranged by size:
4090 2437 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2603 2861 3078 4732 1353
Size: 917 333 258 217 1654 3423
Fragments arranged by size:
3423 1654 917 333 258 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5389 5763 5909 387
Size: 1064 138 3800 374 146 1280
Fragments arranged by size:
3800 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 6802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2763 3239 3508 3760 3965 3973 4865
Size: 393 1131 476 269 252 205 8 892
Cuts at: 4865 5462 6249 6517 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1131 892 787 597 476 393 269
268 252 205 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2778 2778
Size: 6802
EcoO109I rG'GnC_Cy
Cuts at: 2212 2626 2212
Size: 414 6388
EcoRI G'AATT_C
Cuts at: 3902 4096 4111 4489 3902
Size: 194 15 378 6215
Fragments arranged by size:
6215 378 194 15
EcoRV GAT'ATC
Cuts at: 2240 2240
Size: 6802
FspI TGC'GCA
Cuts at: 5308 5308
Size: 6802
HaeII r_GCGC'y
Cuts at: 1678 2896 5949 1678
Size: 1218 3053 2531
Fragments arranged by size:
3053 2531 1218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5443 5475 5517 5549 1373
Size: 32 135 32 3871 32 42 32 2626
Fragments arranged by size:
3871 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4146 4392 669
Size: 988 281 2208 246 3079
Fragments arranged by size:
3079 2208 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 5256
HpaI GTT'AAC
Cuts at: 4392 4392
Size: 6802
KpnI G_GTAC'C
Cuts at: 4127 4127
Size: 6802
MfeI C'AATT_G
Cuts at: 4401 4401
Size: 6802
MluI A'CGCG_T
Cuts at: 3677 6678 3677
Size: 3001 3801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 5916 6100 1305
Size: 1296 3315 184 2007
Fragments arranged by size:
3315 2007 1296 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2547 3192 10
Size: 54 1425 343 195 520 645 3620
Fragments arranged by size:
3620 1425 645 520 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3999 4760 5119 5278 518
Size: 648 2833 761 359 159 2042
Fragments arranged by size:
2833 2042 761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2738 2963 3563 3592 4067 4218 4895 6043
Size: 1132 225 600 29 475 151 677 1148
Cuts at: 6043 6288 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1132 677 600 475 245 225
151 29
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6250
NdeI CA'TA_TG
Cuts at: 387 2719 387
Size: 2332 4470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6802
NotI GC'GGCC_GC
Cuts at: 4249 4249
Size: 6802
NsiI A_TGCA'T
Cuts at: 2719 2719
Size: 6802
NspI r_CATG'y
Cuts at: 1132 1229 2522 4160 5705 1132
Size: 97 1293 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 1293 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6802
PciI A'CATG_T
Cuts at: 2518 5701 2518
Size: 3183 3619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2751 3800 1108
Size: 1643 1049 4110
Fragments arranged by size:
4110 1643 1049
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 6802
PmeI GTTT'AAAC
Cuts at: 4106 4106
Size: 6802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4871 4903 6384 6416 3368
Size: 32 1471 32 1481 32 3754
Fragments arranged by size:
3754 1481 1471 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2626 2212
Size: 414 6388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6802
PsiI TTA'TAA
Cuts at: 4372 4372
Size: 6802
PstI C_TGCA'G
Cuts at: 830 2791 2901 4154 830
Size: 1961 110 1253 3478
Fragments arranged by size:
3478 1961 1253 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6802
PvuII CAG'CTG
Cuts at: 2738 2963 3563 4067 2738
Size: 225 600 504 5473
Fragments arranged by size:
5473 600 504 225
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 6802
SacII CC_GC'GG
Cuts at: 3593 3593
Size: 6802
SalI G'TCGA_C
Cuts at: 1936 4144 1936
Size: 2208 4594
SbfI CC_TGCA'GG
Cuts at: 2901 4154 2901
Size: 1253 5549
ScaI AGT'ACT
Cuts at: 2541 3529 5050 6762 2541
Size: 988 1521 1712 2581
Fragments arranged by size:
2581 1712 1521 988
SfcI C'TryA_G
Cuts at: 826 1045 2787 2817 2897 4150 5285 5966 6157
Size: 219 1742 30 80 1253 1135 681 191
Cuts at: 6157 826
Size: 1471
Fragments arranged by size:
1742 1471 1253 1135 681 219 191 80
30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2033 2033
Size: 6802
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6802
SmaI CCC'GGG
Cuts at: 1872 3557 4129 1872
Size: 1685 572 4545
Fragments arranged by size:
4545 1685 572
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 4906 5807 6069 820
Size: 197 926 615 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 4593 493
Size: 4100 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6802
SphI G_CATG'C
Cuts at: 1132 4160 1132
Size: 3028 3774
SspI AAT'ATT
Cuts at: 5 52 3893 4726 5
Size: 47 3841 833 2081
Fragments arranged by size:
3841 2081 833 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 5730
StyI C'CwwG_G
Cuts at: 513 1065 2403 3866 4088 4241 513
Size: 552 1338 1463 222 153 3074
Fragments arranged by size:
3074 1463 1338 552 222 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3634 4829 4846 4999 5184 5629 325
Size: 1255 2054 1195 17 153 185 445 1498
Fragments arranged by size:
2054 1498 1255 1195 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2539 3527 3967
Size: 274 80 33 51 1443 562 988 440
Cuts at: 3967 5048 6760 96
Size: 1081 1712 138
Fragments arranged by size:
1712 1443 1081 988 562 440 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6762 6794 6762
Size: 32 6770
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6802
XbaI T'CTAG_A
Cuts at: 4138 4138
Size: 6802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3200 3200
Size: 6802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6802
XmaI C'CCGG_G
Cuts at: 1870 3555 4127 1870
Size: 1685 572 4545
Fragments arranged by size:
4545 1685 572
XmnI GAAnn'nnTTC
Cuts at: 3514 3892 4044 4077 4931 3514
Size: 378 152 33 854 5385
Fragments arranged by size:
5385 854 378 152 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4610 276
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfoI SrfI SwaI