Restriction map for
pFN21AB3382
(Circular) (Six-base) MAPSORT of: pFN21AB3382.seq Check: 9249 from: 1 to: 5968
pFN21AB3382 5968 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2494 2494
Size: 5968
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4031 5415 5683 1632
Size: 2399 1384 268 1917
Fragments arranged by size:
2399 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 2784 4867 5844 1203
Size: 1581 2083 977 1327
Fragments arranged by size:
2083 1581 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 2675 4697 2675
Size: 2022 3946
AleI CACnn'nnGTG
Cuts at: 1166 2315 1166
Size: 1149 4819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 5936
AlwNI CAG_nnn'CTG
Cuts at: 3711 5283 3711
Size: 1572 4396
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2875 3277 3503 3655 5764 1493
Size: 1382 402 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 402 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 3293 1870
Size: 73 1350 4545
Fragments arranged by size:
4545 1350 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2360 2393 2360
Size: 33 5935
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2494 2747 3119 3289
Size: 325 217 627 507 200 253 372 170
Cuts at: 3289 4744 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 507 372 325 253 217
200 170
BanII G_rGCy'C
Cuts at: 1391 2327 2438 2478 3167 3287 1391
Size: 936 111 40 689 120 4072
Fragments arranged by size:
4072 936 689 120 111 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2416 928
Size: 971 517 4480
Fragments arranged by size:
4480 971 517
BbvCI CC'TCA_GC
Cuts at: 2220 3000 2220
Size: 780 5188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 3860 5070 2203
Size: 1657 1210 3101
Fragments arranged by size:
3101 1657 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2616 2658 4579
Size: 107 122 71 631 605 944 42 1921
Cuts at: 4579 136
Size: 1525
Fragments arranged by size:
1921 1525 944 631 605 122 107 71
42
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 2299 2834 3122 3140 4029 5185 1790
Size: 509 535 288 18 889 1156 2573
Fragments arranged by size:
2573 1156 889 535 509 288 18
BmgBI CAC'GTC
Cuts at: 1318 2137 1318
Size: 819 5149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3699 4657 442
Size: 416 53 83 399 24 2282 958 1753
Fragments arranged by size:
2282 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3013 3181 3212 4628 1239
Size: 1774 168 31 1416 2579
Fragments arranged by size:
2579 1774 1416 168 31
Bpu10I CC'TnA_GC
Cuts at: 2220 3000 2220
Size: 780 5188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4093 4958 5256 4093
Size: 865 298 4805
Fragments arranged by size:
4805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2083 4631 882
Size: 617 584 2548 2219
Fragments arranged by size:
2548 2219 617 584
BsaAI yAC'GTr
Cuts at: 493 1204 2722 2785 3759 493
Size: 711 1518 63 974 2702
Fragments arranged by size:
2702 1518 974 711 63
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3775 4157 4792
Size: 53 83 186 936 1623 619 382 635
Cuts at: 4792 275
Size: 1451
Fragments arranged by size:
1623 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2200 4401 4799 5073 5220 5631 2200
Size: 2201 398 274 147 411 2537
Fragments arranged by size:
2537 2201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3017 3037 1186
Size: 1831 20 4117
Fragments arranged by size:
4117 1831 20
BseYI C'CCAG_C
Cuts at: 2041 2146 2152 2225 2980 3133 5171 5667 2041
Size: 105 6 73 755 153 2038 496 2342
Fragments arranged by size:
2342 2038 755 496 153 105 73 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2522 2528 1163
Size: 573 498 288 6 4603
Fragments arranged by size:
4603 573 498 288 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4029 4114 5185 4029
Size: 85 1071 4812
Fragments arranged by size:
4812 1071 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 2327 2438 2478 2834 3122 3140
Size: 399 509 28 111 40 356 288 18
Cuts at: 3140 3167 3287 4029 4114 5185 1391
Size: 27 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 399 356 288 120
111 85 40 28 27 18
BspEI T'CCGG_A
Cuts at: 2200 5631 2200
Size: 3431 2537
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2494 3323 844
Size: 1650 829 3489
Fragments arranged by size:
3489 1650 829
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 4463 4637 65
Size: 1183 1042 2173 174 1396
Fragments arranged by size:
2173 1396 1183 1042 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 2652 4612 4799 1700
Size: 110 372 470 1960 187 2869
Fragments arranged by size:
2869 1960 470 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 2576 1184
Size: 1392 4576
BstXI CCAn_nnnn'nTGG
Cuts at: 2819 2819
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963
Size: 1326 367 385 17 1452 86 12 346
Cuts at: 5963 1972
Size: 1977
Fragments arranged by size:
1977 1452 1326 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2539 2539
Size: 5968
BtgI C'CryG_G
Cuts at: 513 1065 2644 513
Size: 552 1579 3837
Fragments arranged by size:
3837 1579 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2629 2664 529
Size: 35 2065 35 3833
Fragments arranged by size:
3833 2065 35 35
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DraIII CAC_nnn'GTG
Cuts at: 2242 2244 2557 2242
Size: 2 313 5653
Fragments arranged by size:
5653 313 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 2416 2650
Size: 54 1425 50 275 18 430 156 234
Cuts at: 2650 2908 3415 4304 5933 8
Size: 258 507 889 1629 43
Fragments arranged by size:
1629 1425 889 507 430 275 258 234
156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 3002 3898 1353
Size: 1127 522 896 3423
Fragments arranged by size:
3423 1127 896 522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3013 3181 3212 4031 4628 5415 5683
Size: 393 1381 168 31 819 597 787 268
Cuts at: 5683 1239
Size: 1524
Fragments arranged by size:
1524 1381 819 787 597 393 268 168
31
EcoNI CCTnn'n_nnAGG
Cuts at: 2990 2990
Size: 5968
EcoO109I rG'GnC_Cy
Cuts at: 2034 2088 3063 2034
Size: 54 975 4939
Fragments arranged by size:
4939 975 54
EcoRI G'AATT_C
Cuts at: 2875 3277 3655 2875
Size: 402 378 5188
Fragments arranged by size:
5188 402 378
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 5115 1678
Size: 3437 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2661 2693 4609 4641 4683
Size: 32 135 32 1089 32 1916 32 42
Cuts at: 4683 4715 1373
Size: 32 2626
Fragments arranged by size:
2626 1916 1089 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 2829 5082 5266 1305
Size: 1146 378 2253 184 2007
Fragments arranged by size:
2253 2007 1146 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 2910 10
Size: 54 1425 343 430 648 3068
Fragments arranged by size:
3068 1425 648 430 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3926 4285 4444 518
Size: 648 1149 1611 359 159 2042
Fragments arranged by size:
2042 1611 1149 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2512 2570 3133 3190 3222 3384 4061
Size: 540 366 58 563 57 32 162 677
Cuts at: 4061 5209 5454 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 563 540 366 245 162
58 57 32
NaeI GCC'GGC
Cuts at: 2184 2654 2184
Size: 470 5498
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NgoMIV G'CCGG_C
Cuts at: 2182 2652 2182
Size: 470 5498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2236 3326 4871 1132
Size: 97 1007 1090 1545 2229
Fragments arranged by size:
2229 1545 1090 1007 97
PasI CC'CwG_GG
Cuts at: 1348 2299 2339 1348
Size: 951 40 4977
Fragments arranged by size:
4977 951 40
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2889 1108
Size: 1781 4187
PfoI T'CCnGG_A
Cuts at: 2442 2730 2442
Size: 288 5680
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PmlI CAC'GTG
Cuts at: 2722 2785 2722
Size: 63 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2088 2034
Size: 54 5914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 3320 830
Size: 2490 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2146 2512 2570 3133 3190 2146
Size: 366 58 563 57 4924
Fragments arranged by size:
4924 563 366 58 57
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2480 3002 2480
Size: 522 5446
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 3316 4451 5132 5323 826
Size: 219 2271 1135 681 191 1471
Fragments arranged by size:
2271 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2616 2658 2616
Size: 42 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4072 4973 5235 820
Size: 197 926 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 2236 3326 1132
Size: 1104 1090 3774
Fragments arranged by size:
3774 1104 1090
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 2661 1517
Size: 1144 4824
StyI C'CwwG_G
Cuts at: 513 1065 2589 2986 3087 3407 513
Size: 552 1524 397 101 320 3074
Fragments arranged by size:
3074 1524 552 397 320 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2144 2881 3995 4012 4165 4350
Size: 1255 446 118 737 1114 17 153 185
Cuts at: 4350 4795 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1114 737 446 445 185 153
118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4214 5926 96
Size: 274 80 33 51 1443 2237 1712 138
Fragments arranged by size:
2237 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2890 3130 2890
Size: 240 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfoI SrfI SwaI