Restriction map for
pFN21AB3373
(Circular) (Six-base) MAPSORT of: pFN21AB3373.seq Check: 2372 from: 1 to: 6562
pFN21AB3373 6562 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2068 3114 3905 1937
Size: 131 1046 791 4594
Fragments arranged by size:
4594 1046 791 131
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3512 3615 3804 4625 6009 6277 1632
Size: 527 1353 103 189 821 1384 268 1917
Fragments arranged by size:
1917 1384 1353 821 527 268 189 103
AflII C'TTAA_G
Cuts at: 820 1017 2926 3835 820
Size: 197 1909 909 3547
Fragments arranged by size:
3547 1909 909 197
AflIII A'CryG_T
Cuts at: 1203 3843 5461 6438 1203
Size: 2640 1618 977 1327
Fragments arranged by size:
2640 1618 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 3537 5291 3537
Size: 1754 4808
AleI CACnn'nnGTG
Cuts at: 1166 3214 1166
Size: 2048 4514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AlwNI CAG_nnn'CTG
Cuts at: 3232 3406 4305 5877 3232
Size: 174 899 1572 3917
Fragments arranged by size:
3917 1572 899 174
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 2144 2635 2741 2774 3222 3692 3871 4097
Size: 651 491 106 33 448 470 179 226
Cuts at: 4097 4249 6358 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 651 491 470 448 226 179
152 106 33
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 3054 3774 3887 1870
Size: 73 1111 720 113 4545
Fragments arranged by size:
4545 1111 720 113 73
AvrII C'CTAG_G
Cuts at: 2708 2708
Size: 6562
BamHI G'GATC_C
Cuts at: 2608 3283 3892 4259 2608
Size: 675 609 367 4911
Fragments arranged by size:
4911 675 609 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3883 5338 618
Size: 325 217 627 2096 1455 1842
Fragments arranged by size:
2096 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2716 3061 3593 3881 1391
Size: 1325 345 532 288 4072
Fragments arranged by size:
4072 1325 532 345 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4753 4787 1903
Size: 34 2816 34 3678
Fragments arranged by size:
3678 2816 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 2011 2917 1548
Size: 463 906 5193
Fragments arranged by size:
5193 906 463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5173 136
Size: 107 122 71 631 605 3501 1525
Fragments arranged by size:
3501 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2203 2210 6557 2203
Size: 7 4347 2208
Fragments arranged by size:
4347 2208 7
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 3575 4623 5779 1790
Size: 1785 1048 1156 2573
Fragments arranged by size:
2573 1785 1156 1048
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4293 5251 442
Size: 416 53 83 399 24 2876 958 1753
Fragments arranged by size:
2876 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3800
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3091 3428 3658 5222 1239
Size: 1852 337 230 1564 2579
Fragments arranged by size:
2579 1852 1564 337 230
Bpu10I CC'TnA_GC
Cuts at: 2644 2644
Size: 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2274 2333 3233 4687 5552 5850 2274
Size: 59 900 1454 865 298 2986
Fragments arranged by size:
2986 1454 900 865 298 59
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2898 3182 3808 5225 882
Size: 617 1399 284 626 1417 2219
Fragments arranged by size:
2219 1417 1399 626 617 284
BsaAI yAC'GTr
Cuts at: 493 1204 4353 493
Size: 711 3149 2702
Fragments arranged by size:
3149 2702 711
BsaBI GATnn'nnATC
Cuts at: 2733 6556 2733
Size: 3823 2739
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4369 4751 5386 275
Size: 53 83 186 936 2836 382 635 1451
Fragments arranged by size:
2836 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6337
Fragments arranged by size:
6337 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2172 2487 1186
Size: 986 315 5261
Fragments arranged by size:
5261 986 315
BseYI C'CCAG_C
Cuts at: 3041 5765 6261 3041
Size: 2724 496 3342
Fragments arranged by size:
3342 2724 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5989
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4012 4773 5801 1540
Size: 275 186 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3061 4623 4708 5779 3061
Size: 1562 85 1071 3844
Fragments arranged by size:
3844 1562 1071 85
BsmI GAATG_Cn'
Cuts at: 2709 4073 4166 2709
Size: 1364 93 5105
Fragments arranged by size:
5105 1364 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2716 3061 3575 3593 3881 4623 4708
Size: 399 926 345 514 18 288 742 85
Cuts at: 4708 5779 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 926 742 514 399 345 288
85 18
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 4451 6181 4451
Size: 1730 4832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3917 844
Size: 3073 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2399 3708 5057 5231 65
Size: 1183 1151 1309 1349 174 1396
Fragments arranged by size:
1396 1349 1309 1183 1151 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5206 5393 1700
Size: 110 3396 187 2869
Fragments arranged by size:
3396 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3707 794
Size: 2913 3649
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 3313 3313
Size: 6562
BstYI r'GATC_y
Cuts at: 1972 2203 2210 2245 2608 3283 3892 4259 4644
Size: 231 7 35 363 675 609 367 385
Cuts at: 4644 4661 6113 6199 6211 6557 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 675 609 385 367 363 346
231 86 35 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5071
Fragments arranged by size:
5071 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 4078 4872 4892 4078
Size: 794 20 5748
Fragments arranged by size:
5748 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3718 3866 3718
Size: 148 6414
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 4009 4898
Size: 54 1425 50 275 18 1261 918 889
Cuts at: 4898 6527 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1261 918 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 3136 4492 1353
Size: 983 800 1356 3423
Fragments arranged by size:
3423 1356 983 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5149 5523 5669 387
Size: 1064 138 3560 374 146 1280
Fragments arranged by size:
3560 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3059 3059
Size: 6562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 3091 3428 3512 3615 3658 3804
Size: 393 527 932 337 84 103 43 146
Cuts at: 3804 4625 5222 6009 6277 1239
Size: 821 597 787 268 1524
Fragments arranged by size:
1524 932 821 787 597 527 393 337
268 146 103 84 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2502 3106 2057
Size: 445 604 5513
Fragments arranged by size:
5513 604 445
EcoO109I rG'GnC_Cy
Cuts at: 2471 2871 2471
Size: 400 6162
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
EcoRV GAT'ATC
Cuts at: 3454 3454
Size: 6562
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 3366 5709 1678
Size: 1688 2343 2531
Fragments arranged by size:
2531 2343 1688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2600 2632 2893
Size: 32 135 32 623 32 373 32 261
Cuts at: 2893 2925 3523 3555 5203 5235 5277 5309 1373
Size: 32 598 32 1648 32 42 32 2626
Fragments arranged by size:
2626 1648 623 598 373 261 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2069 3906 4152 669
Size: 988 281 131 1837 246 3079
Fragments arranged by size:
3079 1837 988 281 246 131
HindIII A'AGCT_T
Cuts at: 748 2019 748
Size: 1271 5291
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3086 5676 5860 1305
Size: 1781 2590 184 2007
Fragments arranged by size:
2590 2007 1781 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3093 10
Size: 54 1425 343 1261 3479
Fragments arranged by size:
3479 1425 1261 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3214 3311 3848 4520 4879 5038 518
Size: 648 2048 97 537 672 359 159 2042
Fragments arranged by size:
2048 2042 672 648 537 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 3824 3978 4655 5803 6048 1606
Size: 2218 154 677 1148 245 2120
Fragments arranged by size:
2218 2120 1148 677 245 154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5071
Fragments arranged by size:
5071 939 552
NdeI CA'TA_TG
Cuts at: 387 2337 3358 387
Size: 1950 1021 3591
Fragments arranged by size:
3591 1950 1021
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 3651 3847 3920 5465 1132
Size: 97 2422 196 73 1545 2229
Fragments arranged by size:
2422 2229 1545 196 97 73
PasI CC'CwG_GG
Cuts at: 1348 2875 1348
Size: 1527 5035
PciI A'CATG_T
Cuts at: 3843 5461 3843
Size: 1618 4944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 5065
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2471 2871 2471
Size: 400 6162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspXI vC'TCGA_Gb
Cuts at: 3054 3054
Size: 6562
PstI C_TGCA'G
Cuts at: 830 3914 830
Size: 3084 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 3824 3824
Size: 6562
SacI G_AGCT'C
Cuts at: 3061 3061
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 2067 3904 1936
Size: 131 1837 4594
Fragments arranged by size:
4594 1837 131
SapI GCTCTTCn'nnn_
Cuts at: 2336 2336
Size: 6562
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 4810 6522 4810
Size: 1712 4850
SexAI A'CCwGG_T
Cuts at: 2529 2529
Size: 6562
SfcI C'TryA_G
Cuts at: 826 1045 3910 5045 5726 5917 826
Size: 219 2865 1135 681 191 1471
Fragments arranged by size:
2865 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2289 2348 2926 3054 3212 3774
Size: 197 926 346 59 578 128 158 562
Cuts at: 3774 3835 4666 5567 5829 820
Size: 61 831 901 262 1553
Fragments arranged by size:
1553 926 901 831 578 562 346 262
197 158 128 61 59
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 2477 2555 2933 152
Size: 2325 78 378 3781
Fragments arranged by size:
3781 2325 378 78
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6562
StyI C'CwwG_G
Cuts at: 513 1065 2004 2708 3094 3683 4001 513
Size: 552 939 704 386 589 318 3074
Fragments arranged by size:
3074 939 704 589 552 386 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3055 4589 4606 4759 4944 5389 325
Size: 1255 1475 1534 17 153 185 445 1498
Fragments arranged by size:
1534 1498 1475 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2375 3650 4808
Size: 274 80 33 51 1443 398 1275 1158
Cuts at: 4808 6520 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1275 1158 398 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6522 6554 6522
Size: 32 6530
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 3073 3101 2266
Size: 807 28 5727
Fragments arranged by size:
5727 807 28
XhoI C'TCGA_G
Cuts at: 1943 3054 3774 1943
Size: 1111 720 4731
Fragments arranged by size:
4731 1111 720
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 4691 4691
Size: 6562
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI