Restriction map for
pFN21AB3349
(Circular) (Six-base) MAPSORT of: pFN21AB3349.seq Check: 3051 from: 1 to: 9286
pFN21AB3349 9286 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3532 4792 5698 6222 3532
Size: 1260 906 524 6596
Fragments arranged by size:
6596 1260 906 524
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7096 278
Size: 53 83 186 936 5560 2468
Fragments arranged by size:
5560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 5014 6629 1937
Size: 673 2404 1615 4594
Fragments arranged by size:
4594 2404 1615 673
Acc65I G'GTAC_C
Cuts at: 6607 6607
Size: 9286
AclI AA'CG_TT
Cuts at: 1563 7413 7786 1563
Size: 5850 373 3063
Fragments arranged by size:
5850 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2557 2805 2924 3527 3557 4103 5432 7349
Size: 925 248 119 603 30 546 1329 1917
Cuts at: 7349 8733 9001 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1917 1384 1329 925 603 546 268
248 119 30
AfeI AGC'GCT
Cuts at: 2638 3260 4160 5384 2638
Size: 622 900 1224 6540
Fragments arranged by size:
6540 1224 900 622
AflII C'TTAA_G
Cuts at: 820 1017 3736 820
Size: 197 2719 6370
Fragments arranged by size:
6370 2719 197
AflIII A'CryG_T
Cuts at: 1203 3666 6219 8185 9162 1203
Size: 2463 2553 1966 977 1327
Fragments arranged by size:
2553 2463 1966 1327 977
AgeI A'CCGG_T
Cuts at: 5580 8117 5580
Size: 2537 6749
AhdI GACnn_n'nnGTC
Cuts at: 2725 4336 8015 2725
Size: 1611 3679 3996
Fragments arranged by size:
3996 3679 1611
AleI CACnn'nnGTG
Cuts at: 1166 4694 5306 5375 6224 1166
Size: 3528 612 69 849 4228
Fragments arranged by size:
4228 3528 849 612 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3518 1184
Size: 2334 6952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5012 5044 5204 5236 5858 5890 5012
Size: 32 160 32 622 32 8408
Fragments arranged by size:
8408 622 160 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2770 2773 2831 2949 2971 3097 3577 3922 4174
Size: 3 58 118 22 126 480 345 252
Cuts at: 4174 4986 5146 5471 7029 8601 2770
Size: 812 160 325 1558 1572 3455
Fragments arranged by size:
3455 1572 1558 812 480 345 325 252
160 126 118 58 22 3
ApaI G_GGCC'C
Cuts at: 2305 3916 4505 2305
Size: 1611 589 7086
Fragments arranged by size:
7086 1611 589
ApaLI G'TGCA_C
Cuts at: 2066 4058 5942 7343 8499 2066
Size: 1992 1884 1401 1156 2853
Fragments arranged by size:
2853 1992 1884 1401 1156
ApoI r'AATT_y
Cuts at: 1493 5061 6512 6595 6821 6973 9082 1493
Size: 3568 1451 83 226 152 2109 1697
Fragments arranged by size:
3568 2109 1697 1451 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 7840 160
Size: 872 6808 1606
Fragments arranged by size:
6808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9286
AvaI C'yCGr_G
Cuts at: 1870 1943 4290 5671 6133 6315 6611 1870
Size: 73 2347 1381 462 182 296 4545
Fragments arranged by size:
4545 2347 1381 462 296 182 73
AvrII C'CTAG_G
Cuts at: 3346 3346
Size: 9286
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5520 5553 5520
Size: 33 9253
BamHI G'GATC_C
Cuts at: 3807 4101 6616 6983 3807
Size: 294 2515 367 6110
Fragments arranged by size:
6110 2515 367 294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2175 2870 3899 4581
Size: 325 217 627 335 53 695 1029 682
Cuts at: 4581 4776 5474 5812 6607 8062 618
Size: 195 698 338 795 1455 1842
Fragments arranged by size:
1842 1455 1029 795 698 695 682 627
338 335 325 217 195 53
BanII G_rGCy'C
Cuts at: 1391 2034 2305 3528 3570 3916 4073 4505 4872
Size: 643 271 1223 42 346 157 432 367
Cuts at: 4872 4908 6233 6322 6605 1391
Size: 36 1325 89 283 4072
Fragments arranged by size:
4072 1325 1223 643 432 367 346 283
271 157 89 42 36
BbeI G_GCGC'C
Cuts at: 5816 5816
Size: 9286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 4650 5985 928
Size: 971 495 2256 1335 4229
Fragments arranged by size:
4229 2256 1335 971 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2165 2199 7477 7511 1903
Size: 34 228 34 5278 34 3678
Fragments arranged by size:
5278 3678 228 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7178 8388 7178
Size: 1210 8076
BclI T'GATC_A
Cuts at: 1548 3009 3648 1548
Size: 1461 639 7186
Fragments arranged by size:
7186 1461 639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2915 3704 7897
Size: 107 122 71 631 605 1243 789 4193
Cuts at: 7897 136
Size: 1525
Fragments arranged by size:
4193 1525 1243 789 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2496 9281 2496
Size: 6785 2501
BlpI GC'TnA_GC
Cuts at: 2999 5588 6703 2999
Size: 2589 1115 5582
Fragments arranged by size:
5582 2589 1115
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2127 2305 2875 3916 3970 4062 4089
Size: 280 57 178 570 1041 54 92 27
Cuts at: 4089 4505 4584 4732 4831 5946 7347 8503 1790
Size: 416 79 148 99 1115 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 1115 1041 570 416 280
178 148 99 92 79 57 54 27
BmgBI CAC'GTC
Cuts at: 1318 4381 1318
Size: 3063 6223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 3162 3757
Size: 416 53 83 399 24 1727 18 595
Cuts at: 3757 5988 6409 7017 7975 442
Size: 2231 421 608 958 1753
Fragments arranged by size:
2231 1753 1727 958 608 595 421 416
399 83 53 24 18
BmtI G_CTAG'C
Cuts at: 1056 4196 4478 1056
Size: 3140 282 5864
Fragments arranged by size:
5864 3140 282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 6341 2053
Size: 4288 4998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 2795 2914 3387 3554 4196 4223 4607
Size: 1490 66 119 473 167 642 27 384
Cuts at: 4607 6044 6398 6507 7946 1239
Size: 1437 354 109 1439 2579
Fragments arranged by size:
2579 1490 1439 1437 642 473 384 354
167 119 109 66 27
Bpu10I CC'TnA_GC
Cuts at: 3821 3821
Size: 9286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 7411 8276 8574 2204
Size: 5207 865 298 2916
Fragments arranged by size:
5207 2916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2624 5919 7949 882
Size: 617 1125 3295 2030 2219
Fragments arranged by size:
3295 2219 2030 1125 617
BsaAI yAC'GTr
Cuts at: 493 1204 3667 3673 5861 7077 493
Size: 711 2463 6 2188 1216 2702
Fragments arranged by size:
2702 2463 2188 1216 711 6
BsaBI GATnn'nnATC
Cuts at: 2684 9280 2684
Size: 6596 2690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 3696 5043 5813
Size: 53 83 186 936 477 1686 1347 770
Cuts at: 5813 7093 7475 8110 275
Size: 1280 382 635 1451
Fragments arranged by size:
1686 1451 1347 1280 936 770 635 477
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5580 5877 7719 8117 8391 8538 8949 5580
Size: 297 1842 398 274 147 411 5917
Fragments arranged by size:
5917 1842 411 398 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2960 2990 4956 4986 5204
Size: 30 165 30 1557 30 1966 30 218
Cuts at: 5204 5234 5282 5312 5558 5588 5858 5888 1178
Size: 30 48 30 246 30 270 30 4576
Fragments arranged by size:
4576 1966 1557 270 246 218 165 48
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 4856 5671 6194 1186
Size: 1540 2130 815 523 4278
Fragments arranged by size:
4278 2130 1540 815 523
BseYI C'CCAG_C
Cuts at: 2866 3062 3255 3527 4027 4089 4510 5038 6234
Size: 196 193 272 500 62 421 528 1196
Cuts at: 6234 8489 8985 2866
Size: 2255 496 3167
Fragments arranged by size:
3167 2255 1196 528 500 496 421 272
196 193 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2783 3646 3920 4096 4635 4820 5399
Size: 573 1047 863 274 176 539 185 579
Cuts at: 5399 5619 6200 6412 1163
Size: 220 581 212 4037
Fragments arranged by size:
4037 1047 863 581 579 573 539 274
220 212 185 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5903 6736 7497 8525 1540
Size: 275 186 3902 833 761 1028 2301
Fragments arranged by size:
3902 2301 1028 833 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2070 3083 3570 4062 4872 5367 5628 5946
Size: 36 1013 487 492 810 495 261 318
Cuts at: 5946 6233 6263 7347 7432 8503 2034
Size: 287 30 1084 85 1071 2817
Fragments arranged by size:
2817 1084 1071 1013 810 495 492 487
318 287 261 85 36 30
BsmI GAATG_Cn'
Cuts at: 6797 6890 6797
Size: 93 9193
BsmBI CGTCTCn'nnnn_
Cuts at: 2257 2257
Size: 9286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2070 2127 2305 2875 3083 3528
Size: 399 244 36 57 178 570 208 445
Cuts at: 3528 3570 3916 3970 4062 4073 4089 4505 4584
Size: 42 346 54 92 11 16 416 79
Cuts at: 4584 4732 4831 4872 4908 5367 5628 5946 6233
Size: 148 99 41 36 459 261 318 287
Cuts at: 6233 6263 6322 6605 7347 7432 8503 1391
Size: 30 59 283 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 459 445 416 399
346 318 287 283 261 244 208 178
148 99 92 85 79 59 57 54
42 41 36 36 30 16 11
BspEI T'CCGG_A
Cuts at: 5877 8949 5877
Size: 3072 6214
BspHI T'CATG_A
Cuts at: 7175 8905 7175
Size: 1730 7556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2979 3532 3930 4792 5698 6096 6222
Size: 1334 801 553 398 862 906 398 126
Cuts at: 6222 6434 6641 844
Size: 212 207 3489
Fragments arranged by size:
3489 1334 906 862 801 553 398 398
212 207 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2383 2463 5332 5436 7173 1394
Size: 429 560 80 2869 104 1737 3507
Fragments arranged by size:
3507 2869 1737 560 429 104 80
BsrDI GCAATG_nn'
Cuts at: 65 1248 6534 7781 7955 65
Size: 1183 5286 1247 174 1396
Fragments arranged by size:
5286 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 5580 5898 6238 6450 7930 8117
Size: 110 887 2883 318 340 212 1480 187
Cuts at: 8117 1700
Size: 2869
Fragments arranged by size:
2883 2869 1480 887 340 318 212 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9286
BssHII G'CGCG_C
Cuts at: 1925 3424 1925
Size: 1499 7787
BssSI C'ACGA_G
Cuts at: 6302 7346 8358 6302
Size: 1044 1012 7230
Fragments arranged by size:
7230 1044 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2770 5642 794
Size: 1976 2872 4438
Fragments arranged by size:
4438 2872 1976
BstBI TT'CG_AA
Cuts at: 8090 8090
Size: 9286
BstEII G'GTnAC_C
Cuts at: 1184 2344 3640 1184
Size: 1160 1296 6830
Fragments arranged by size:
6830 1296 1160
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2356 3031 4900 5101 5308 6376 6484 2186
Size: 170 675 1869 201 207 1068 108 4988
Fragments arranged by size:
4988 1869 1068 675 207 201 170 108
BstYI r'GATC_y
Cuts at: 1972 2469 2496 2747 3483 3807 4101 4666 6616
Size: 497 27 251 736 324 294 565 1950
Cuts at: 6616 6983 7368 7385 8837 8923 8935 9281 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1950 1452 736 565 497 385 367
346 324 294 251 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 5033 5033
Size: 9286
BtgI C'CryG_G
Cuts at: 513 1065 2004 2281 2293 3030 3978 4074 4341
Size: 552 939 277 12 737 948 96 267
Cuts at: 4341 4899 5001 5094 5100 513
Size: 558 102 93 6 4699
Fragments arranged by size:
4699 948 939 737 558 552 277 267
102 96 93 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6427 487
Size: 683 56 5201 3346
Fragments arranged by size:
5201 3346 683 56
BtsI GCAGTG_nn'
Cuts at: 4631 5144 6802 7596 7616 4631
Size: 513 1658 794 20 6301
Fragments arranged by size:
6301 1658 794 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9251
DraI TTT'AAA
Cuts at: 6590 6590
Size: 9286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3953 5234 8293 809
Size: 507 2637 1281 3059 1802
Fragments arranged by size:
3059 2637 1802 1281 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 2358 2682
Size: 54 1425 50 275 18 466 62 324
Cuts at: 2682 2916 3411 3975 4359 4920 5097 5319 5900
Size: 234 495 564 384 561 177 222 581
Cuts at: 5900 6564 6733 7622 9251 8
Size: 664 169 889 1629 43
Fragments arranged by size:
1629 1425 889 664 581 564 561 495
466 384 324 275 234 222 177 169
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5900 6733 1537
Size: 275 4088 833 4090
Fragments arranged by size:
4090 4088 833 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 4122 5339 7216 1353
Size: 1380 1389 1217 1877 3423
Fragments arranged by size:
3423 1877 1389 1380 1217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2276 7873 8247 8393 387
Size: 1064 138 687 5597 374 146 1280
Fragments arranged by size:
5597 1280 1064 687 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 3568 4870 6231 2032
Size: 1536 1302 1361 5087
Fragments arranged by size:
5087 1536 1361 1302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2557 2729 2795 2805 2914 2924 3387
Size: 393 925 172 66 10 109 10 463
Cuts at: 3387 3527 3554 3557 4103 4196 4223 4607 5432
Size: 140 27 3 546 93 27 384 825
Cuts at: 5432 6044 6398 6507 7349 7946 8733 9001 1239
Size: 612 354 109 842 597 787 268 1524
Fragments arranged by size:
1524 925 842 825 787 612 597 546
463 393 384 354 268 172 140 109
109 93 66 27 27 10 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2572 3539 3782 5844 6395 2572
Size: 967 243 2062 551 5463
Fragments arranged by size:
5463 2062 967 551 243
EcoO109I rG'GnC_Cy
Cuts at: 2302 2410 2719 2883 2908 2968 3350 3919 3955
Size: 108 309 164 25 60 382 569 36
Cuts at: 3955 3986 6118 6138 6481 2302
Size: 31 2132 20 343 5107
Fragments arranged by size:
5107 2132 569 382 343 309 164 108
60 36 31 25 20
EcoRI G'AATT_C
Cuts at: 5061 6595 6973 5061
Size: 1534 378 7374
Fragments arranged by size:
7374 1534 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 3851 4387 2914
Size: 937 536 7813
Fragments arranged by size:
7813 937 536
FspI TGC'GCA
Cuts at: 3581 6146 7792 3581
Size: 2565 1646 5075
Fragments arranged by size:
5075 2565 1646
HaeII r_GCGC'y
Cuts at: 1678 2640 3262 4162 5386 5816 8433 1678
Size: 962 622 900 1224 430 2617 2531
Fragments arranged by size:
2617 2531 1224 962 900 622 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2711 2732 2743 2764 2960
Size: 32 135 32 1139 21 11 21 196
Cuts at: 2960 2992 3732 3764 4322 4354 5704 5736 5741
Size: 32 740 32 558 32 1350 32 5
Cuts at: 5741 5773 6077 6109 7927 7959 8001 8033 1373
Size: 32 304 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 1350 1139 740 558 304 196
135 42 32 32 32 32 32 32
32 32 32 32 21 21 11 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 3235 6630 6876 669
Size: 988 281 673 624 3395 246 3079
Fragments arranged by size:
3395 3079 988 673 624 281 246
HindIII A'AGCT_T
Cuts at: 748 2957 748
Size: 2209 7077
HpaI GTT'AAC
Cuts at: 6876 6876
Size: 9286
KasI G'GCGC_C
Cuts at: 5812 5812
Size: 9286
KpnI G_GTAC'C
Cuts at: 6611 6611
Size: 9286
MfeI C'AATT_G
Cuts at: 6885 6885
Size: 9286
MluI A'CGCG_T
Cuts at: 9162 9162
Size: 9286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 3048 4587 5601 8400 8584 1305
Size: 774 969 1539 1014 2799 184 2007
Fragments arranged by size:
2799 2007 1539 1014 969 774 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 2684 2918 4361 4922
Size: 54 1425 343 528 324 234 1443 561
Cuts at: 4922 5099 5321 6566 10
Size: 177 222 1245 2730
Fragments arranged by size:
2730 1443 1425 1245 561 528 343 324
234 222 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 2678 3128 3245 3500 3620 4469
Size: 648 1018 494 450 117 255 120 849
Cuts at: 4469 4694 5099 5267 5306 5375 5597 6224 6464
Size: 225 405 168 39 69 222 627 240
Cuts at: 6464 7244 7603 7762 518
Size: 780 359 159 2042
Fragments arranged by size:
2042 1018 849 780 648 627 494 450
405 359 255 240 225 222 168 159
120 117 69 39
MspA1I CmG'CkG
Cuts at: 1606 2770 2995 3574 4270 4276 4343 4596 4723
Size: 1164 225 579 696 6 67 253 127
Cuts at: 4723 5108 6100 6365 6702 7379 8527 8772 1606
Size: 385 992 265 337 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 992 696 677 579 385
337 265 253 245 225 127 67 6
NaeI GCC'GGC
Cuts at: 6240 6240
Size: 9286
NarI GG'CG_CC
Cuts at: 5813 5813
Size: 9286
NcoI C'CATG_G
Cuts at: 513 1065 2004 2281 2293 3030 5001 5094 5100
Size: 552 939 277 12 737 1971 93 6
Cuts at: 5100 513
Size: 4699
Fragments arranged by size:
4699 1971 939 737 552 277 93 12
6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9286
NgoMIV G'CCGG_C
Cuts at: 6238 6238
Size: 9286
NheI G'CTAG_C
Cuts at: 1052 4192 4474 1052
Size: 3140 282 5864
Fragments arranged by size:
5864 3140 282
NotI GC'GGCC_GC
Cuts at: 6733 6733
Size: 9286
NspI r_CATG'y
Cuts at: 1132 1229 3757 5942 6644 8189 1132
Size: 97 2528 2185 702 1545 2229
Fragments arranged by size:
2528 2229 2185 1545 702 97
PasI CC'CwG_GG
Cuts at: 1348 5817 1348
Size: 4469 4817
PciI A'CATG_T
Cuts at: 8185 8185
Size: 9286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2046 2287 2758 3850 4974 5583 1108
Size: 938 241 471 1092 1124 609 4811
Fragments arranged by size:
4811 1124 1092 938 609 471 241
PfoI T'CCnGG_A
Cuts at: 2964 6021 6489 2964
Size: 3057 468 5761
Fragments arranged by size:
5761 3057 468
PmeI GTTT'AAAC
Cuts at: 6590 6590
Size: 9286
PmlI CAC'GTG
Cuts at: 5861 5861
Size: 9286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 5858 5890 7355 7387 8868 8900 5204
Size: 32 622 32 1465 32 1481 32 5590
Fragments arranged by size:
5590 1481 1465 622 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2719 2968 3350 3919 3955 6118 6138 6481
Size: 309 249 382 569 36 2163 20 343
Cuts at: 6481 2410
Size: 5215
Fragments arranged by size:
5215 2163 569 382 343 309 249 36
20
PshAI GACnn'nnGTC
Cuts at: 1613 4489 5931 1613
Size: 2876 1442 4968
Fragments arranged by size:
4968 2876 1442
PsiI TTA'TAA
Cuts at: 6856 6856
Size: 9286
PspOMI G'GGCC_C
Cuts at: 2301 3912 4501 2301
Size: 1611 589 7086
Fragments arranged by size:
7086 1611 589
PspXI vC'TCGA_Gb
Cuts at: 4290 4290
Size: 9286
PstI C_TGCA'G
Cuts at: 830 2391 3312 4524 6638 830
Size: 1561 921 1212 2114 3478
Fragments arranged by size:
3478 2114 1561 1212 921
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9286
PvuII CAG'CTG
Cuts at: 2770 2995 3574 4596 4723 6100 6365 2770
Size: 225 579 1022 127 1377 265 5691
Fragments arranged by size:
5691 1377 1022 579 265 225 127
SacI G_AGCT'C
Cuts at: 2034 3570 4872 6233 2034
Size: 1536 1302 1361 5087
Fragments arranged by size:
5087 1536 1361 1302
SacII CC_GC'GG
Cuts at: 4344 4344
Size: 9286
SalI G'TCGA_C
Cuts at: 1936 2609 6628 1936
Size: 673 4019 4594
Fragments arranged by size:
4594 4019 673
SanDI GG'GwC_CC
Cuts at: 6138 6138
Size: 9286
SapI GCTCTTCn'nnn_
Cuts at: 5339 5339
Size: 9286
SbfI CC_TGCA'GG
Cuts at: 6638 6638
Size: 9286
ScaI AGT'ACT
Cuts at: 5792 7534 9246 5792
Size: 1742 1712 5832
Fragments arranged by size:
5832 1742 1712
SexAI A'CCwGG_T
Cuts at: 5913 5913
Size: 9286
SfcI C'TryA_G
Cuts at: 826 1045 2387 3172 3308 4520 6634 7769 8450
Size: 219 1342 785 136 1212 2114 1135 681
Cuts at: 8450 8641 826
Size: 191 1471
Fragments arranged by size:
2114 1471 1342 1212 1135 785 681 219
191 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 2915 2915
Size: 9286
SfoI GGC'GCC
Cuts at: 5814 5814
Size: 9286
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9286
SmaI CCC'GGG
Cuts at: 1872 5673 6613 1872
Size: 3801 940 4545
Fragments arranged by size:
4545 3801 940
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 3736 4290 7390 8291 8553
Size: 197 926 276 1517 554 3100 901 262
Cuts at: 8553 820
Size: 1553
Fragments arranged by size:
3100 1553 1517 926 901 554 276 262
197
SnaBI TAC'GTA
Cuts at: 493 7077 493
Size: 6584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9286
SphI G_CATG'C
Cuts at: 1132 6644 1132
Size: 5512 3774
SspI AAT'ATT
Cuts at: 5 52 7210 5
Size: 47 7158 2081
Fragments arranged by size:
7158 2081 47
StuI AGG'CCT
Cuts at: 1517 3707 4181 6246 1517
Size: 2190 474 2065 4557
Fragments arranged by size:
4557 2190 2065 474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2281 2293 2331 2481 3030 3346
Size: 552 939 277 12 38 150 549 316
Cuts at: 3346 4576 4893 5001 5094 5100 5223 6725 513
Size: 1230 317 108 93 6 123 1502 3074
Fragments arranged by size:
3074 1502 1230 939 552 549 317 316
277 150 123 108 93 38 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 2222 2858 3152 3649 5809 6428
Size: 1255 530 112 636 294 497 2160 619
Cuts at: 6428 7313 7330 7483 7668 8113 325
Size: 885 17 153 185 445 1498
Fragments arranged by size:
2160 1498 1255 885 636 619 530 497
445 294 185 153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5790 7532 9244
Size: 274 80 33 51 1443 3813 1742 1712
Cuts at: 9244 96
Size: 138
Fragments arranged by size:
3813 1742 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 3148 3180 4956 4988 6176 6208 6242
Size: 32 1116 32 1776 32 1188 32 34
Cuts at: 6242 6274 9246 9278 2000
Size: 32 2972 32 2008
Fragments arranged by size:
2972 2008 1776 1188 1116 34 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4379 4755 1316
Size: 3063 376 5847
Fragments arranged by size:
5847 3063 376
XbaI T'CTAG_A
Cuts at: 6622 6622
Size: 9286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 4861 2221
Size: 2640 6646
XhoI C'TCGA_G
Cuts at: 1943 4290 1943
Size: 2347 6939
XmaI C'CCGG_G
Cuts at: 1870 5671 6611 1870
Size: 3801 940 4545
Fragments arranged by size:
4545 3801 940
XmnI GAAnn'nnTTC
Cuts at: 5216 7415 5216
Size: 2199 7087
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7094 276
Size: 53 83 186 936 5560 2468
Fragments arranged by size:
5560 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI
FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI