Restriction map for
pFN21AB3338
(Circular) (Six-base) MAPSORT of: pFN21AB3338.seq Check: 8631 from: 1 to: 8188
pFN21AB3338 8188 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4415 4415
Size: 8188
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5091 5998 278
Size: 53 83 186 936 3555 907 2468
Fragments arranged by size:
3555 2468 936 907 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3660 4767 5295 5531 1937
Size: 1723 1107 528 236 4594
Fragments arranged by size:
4594 1723 1107 528 236
Acc65I G'GTAC_C
Cuts at: 5509 5509
Size: 8188
AclI AA'CG_TT
Cuts at: 1563 6315 6688 1563
Size: 4752 373 3063
Fragments arranged by size:
4752 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 3052 4160 5083 5150 5445 6251 7635
Size: 980 440 1108 923 67 295 806 1384
Cuts at: 7635 7903 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1108 980 923 806 440 295
268 67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7991
AflIII A'CryG_T
Cuts at: 1203 2265 2937 3177 7087 8064 1203
Size: 1062 672 240 3910 977 1327
Fragments arranged by size:
3910 1327 1062 977 672 240
AgeI A'CCGG_T
Cuts at: 3912 7019 3912
Size: 3107 5081
AhdI GACnn_n'nnGTC
Cuts at: 6917 6917
Size: 8188
AleI CACnn'nnGTG
Cuts at: 1166 3314 5219 1166
Size: 2148 1905 4135
Fragments arranged by size:
4135 2148 1905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 8156
AlwNI CAG_nnn'CTG
Cuts at: 2343 2644 2902 3259 3763 4240 4372 4581 5931
Size: 301 258 357 504 477 132 209 1350
Cuts at: 5931 7503 2343
Size: 1572 3028
Fragments arranged by size:
3028 1572 1350 504 477 357 301 258
209 132
ApaI G_GGCC'C
Cuts at: 2740 3274 3442 3631 3820 2740
Size: 534 168 189 189 7108
Fragments arranged by size:
7108 534 189 189 168
ApaLI G'TGCA_C
Cuts at: 6245 7401 6245
Size: 1156 7032
ApoI r'AATT_y
Cuts at: 1493 5497 5723 5875 7984 1493
Size: 4004 226 152 2109 1697
Fragments arranged by size:
4004 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6742 160
Size: 872 5710 1606
Fragments arranged by size:
5710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8188
AvaI C'yCGr_G
Cuts at: 1870 1943 2629 3462 3631 3819 5513 1870
Size: 73 686 833 169 188 1694 4545
Fragments arranged by size:
4545 1694 833 686 188 169 73
AvrII C'CTAG_G
Cuts at: 3494 3494
Size: 8188
BamHI G'GATC_C
Cuts at: 2650 5518 5885 2650
Size: 2868 367 4953
Fragments arranged by size:
4953 2868 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3134 4622 5509 6964 618
Size: 325 217 627 1347 1488 887 1455 1842
Fragments arranged by size:
1842 1488 1455 1347 887 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2390 2740 3164 3274 3442 3631 3820 4181
Size: 999 350 424 110 168 189 189 361
Cuts at: 4181 4221 4265 4388 4398 4501 4616 4749 5263
Size: 40 44 123 10 103 115 133 514
Cuts at: 5263 5338 5507 1391
Size: 75 169 4072
Fragments arranged by size:
4072 999 514 424 361 350 189 189
169 168 133 123 115 110 103 75
44 40 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 5292 5433 928
Size: 971 553 2840 141 3683
Fragments arranged by size:
3683 2840 971 553 141
BbvCI CC'TCA_GC
Cuts at: 4349 4874 4349
Size: 525 7663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4458 4492 6379 6413 1903
Size: 34 2521 34 1887 34 3678
Fragments arranged by size:
3678 2521 1887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3156 6080 7290 3156
Size: 2924 1210 4054
Fragments arranged by size:
4054 2924 1210
BclI T'GATC_A
Cuts at: 1548 4776 1548
Size: 3228 4960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3626 3815 6799
Size: 107 122 71 631 605 1954 189 2984
Cuts at: 6799 136
Size: 1525
Fragments arranged by size:
2984 1954 1525 631 605 189 122 107
71
BglII A'GATC_T
Cuts at: 2997 4885 8183 2997
Size: 1888 3298 3002
Fragments arranged by size:
3298 3002 1888
BlpI GC'TnA_GC
Cuts at: 2917 4848 5605 2917
Size: 1931 757 5500
Fragments arranged by size:
5500 1931 757
Bme1580I G_kGCm'C
Cuts at: 1790 2552 2740 3274 3442 3631 3820 3940 4525
Size: 762 188 534 168 189 189 120 585
Cuts at: 4525 4551 6249 7405 1790
Size: 26 1698 1156 2573
Fragments arranged by size:
2573 1698 1156 762 585 534 189 189
188 168 120 26
BmgBI CAC'GTC
Cuts at: 1318 3616 1318
Size: 2298 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3013 3927 4127
Size: 416 53 83 399 24 1596 914 200
Cuts at: 4127 5919 6877 442
Size: 1792 958 1753
Fragments arranged by size:
1792 1753 1596 958 914 416 399 200
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3236 1056
Size: 2180 6008
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5016 5016
Size: 8188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 3150 4412 4763 5432 6848 1239
Size: 944 967 1262 351 669 1416 2579
Fragments arranged by size:
2579 1416 1262 967 944 669 351
Bpu10I CC'TnA_GC
Cuts at: 3839 4349 4874 3839
Size: 510 525 7153
Fragments arranged by size:
7153 525 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6313 7178 7476 6313
Size: 865 298 7025
Fragments arranged by size:
7025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6851 882
Size: 617 5352 2219
Fragments arranged by size:
5352 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2938 5979 493
Size: 711 1734 3041 2702
Fragments arranged by size:
3041 2702 1734 711
BsaBI GATnn'nnATC
Cuts at: 2453 8182 2453
Size: 5729 2459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 3954 5088 5995
Size: 53 83 186 936 888 1533 1134 907
Cuts at: 5995 6377 7012 275
Size: 382 635 1451
Fragments arranged by size:
1533 1451 1134 936 907 888 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2619 3912 6621 7019 7293 7440 7851 2619
Size: 1293 2709 398 274 147 411 2956
Fragments arranged by size:
2956 2709 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5078 5108 1178
Size: 30 165 30 3675 30 4258
Fragments arranged by size:
4258 3675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 5126 1186
Size: 901 3039 4248
Fragments arranged by size:
4248 3039 901
BseYI C'CCAG_C
Cuts at: 2383 2394 3726 3918 3947 5004 5151 5229 7391
Size: 11 1332 192 29 1057 147 78 2162
Cuts at: 7391 7887 2383
Size: 496 2684
Fragments arranged by size:
2684 2162 1332 1057 496 192 147 78
29 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2225 2318 3527 4175 1163
Size: 573 408 81 93 1209 648 5176
Fragments arranged by size:
5176 1209 648 573 408 93 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5638 6399 7427 1540
Size: 275 186 3637 761 1028 2301
Fragments arranged by size:
3637 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2158 3834 4388 4398 4749 5263 6249 6334 7405
Size: 1676 554 10 351 514 986 85 1071
Cuts at: 7405 2158
Size: 2941
Fragments arranged by size:
2941 1676 1071 986 554 514 351 85
10
BsmI GAATG_Cn'
Cuts at: 5008 5699 5792 5008
Size: 691 93 7404
Fragments arranged by size:
7404 691 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2390 2552 2740 3164 3274 3442
Size: 399 368 232 162 188 424 110 168
Cuts at: 3442 3631 3820 3834 3940 4181 4221 4265 4388
Size: 189 189 14 106 241 40 44 123
Cuts at: 4388 4398 4501 4525 4551 4616 4749 5263 5338
Size: 10 103 24 26 65 133 514 75
Cuts at: 5338 5507 6249 6334 7405 1391
Size: 169 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 424 399 368 241
232 189 189 188 169 168 162 133
123 110 106 103 85 75 65 44
40 26 24 14 10
BspEI T'CCGG_A
Cuts at: 2619 7851 2619
Size: 5232 2956
BspHI T'CATG_A
Cuts at: 6077 7807 6077
Size: 1730 6458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4248 4415 5543 844
Size: 3404 167 1128 3489
Fragments arranged by size:
3489 3404 1128 167
BsrBI CCG'CTC
Cuts at: 1394 1823 6075 1394
Size: 429 4252 3507
Fragments arranged by size:
4252 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3332 5058 5165 6683 6857 65
Size: 1183 2084 1726 107 1518 174 1396
Fragments arranged by size:
2084 1726 1518 1396 1183 174 107
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 3850 3912 6832 7019 1700
Size: 110 406 1634 62 2920 187 2869
Fragments arranged by size:
2920 2869 1634 406 187 110 62
BsrGI T'GTAC_A
Cuts at: 96 5370 96
Size: 5274 2914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8188
BssSI C'ACGA_G
Cuts at: 2144 3047 4059 6248 7260 2144
Size: 903 1012 2189 1012 3072
Fragments arranged by size:
3072 2189 1012 1012 903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4581 794
Size: 3787 4401
BstBI TT'CG_AA
Cuts at: 6992 6992
Size: 8188
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8188
BstXI CCAn_nnnn'nTGG
Cuts at: 3940 5317 3940
Size: 1377 6811
BstYI r'GATC_y
Cuts at: 1972 2135 2595 2650 2757 2997 4885 5518 5885
Size: 163 460 55 107 240 1888 633 367
Cuts at: 5885 6270 6287 7739 7825 7837 8183 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1888 1452 633 460 385 367 346
240 163 107 86 55 17 12
Bsu36I CC'TnA_GG
Cuts at: 2253 2253
Size: 8188
BtgI C'CryG_G
Cuts at: 513 1065 2688 3393 3532 3810 4257 4377 4446
Size: 552 1623 705 139 278 447 120 69
Cuts at: 4446 513
Size: 4255
Fragments arranged by size:
4255 1623 705 552 447 278 139 120
69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3423 487
Size: 683 56 2197 5252
Fragments arranged by size:
5252 2197 683 56
BtsI GCAGTG_nn'
Cuts at: 2886 3515 3521 4278 4591 4705 5430 5704 6498
Size: 629 6 757 313 114 725 274 794
Cuts at: 6498 6518 2886
Size: 20 4556
Fragments arranged by size:
4556 794 757 725 629 313 274 114
20 6
ClaI AT'CG_AT
Cuts at: 1554 4855 1554
Size: 3301 4887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8153
DraI TTT'AAA
Cuts at: 5492 5492
Size: 8188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3959 4340 7195 809
Size: 507 2643 381 2855 1802
Fragments arranged by size:
2855 2643 1802 507 381
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2685 3618
Size: 54 1425 50 275 18 345 510 933
Cuts at: 3618 3807 3870 3897 4254 4890 5635 6524 8153
Size: 189 63 27 357 636 745 889 1629
Cuts at: 8153 8
Size: 43
Fragments arranged by size:
1629 1425 933 889 745 636 510 357
345 275 189 63 54 50 43 27
18
EagI C'GGCC_G
Cuts at: 1537 1812 5635 1537
Size: 275 3823 4090
Fragments arranged by size:
4090 3823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3101 3918 4280 6118 1353
Size: 1748 817 362 1838 3423
Fragments arranged by size:
3423 1838 1748 817 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 6775 7149 7295 387
Size: 1064 138 648 4538 374 146 1280
Fragments arranged by size:
4538 1280 1064 648 374 146 138
EcoICRI GAG'CTC
Cuts at: 4386 4396 4747 5261 4386
Size: 10 351 514 7313
Fragments arranged by size:
7313 514 351 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2612 3052 3150 4160 4412 4763
Size: 393 551 429 440 98 1010 252 351
Cuts at: 4763 5083 5150 5432 5445 6251 6848 7635 7903
Size: 320 67 282 13 806 597 787 268
Cuts at: 7903 1239
Size: 1524
Fragments arranged by size:
1524 1010 806 787 597 551 440 429
393 351 320 282 268 252 98 67
13
EcoNI CCTnn'n_nnAGG
Cuts at: 3242 3493 3785 3242
Size: 251 292 7645
Fragments arranged by size:
7645 292 251
EcoO109I rG'GnC_Cy
Cuts at: 2736 2882 3270 3271 3378 3438 3439 3627 3628
Size: 146 388 1 107 60 1 188 1
Cuts at: 3628 3778 3816 4333 4564 4960 2736
Size: 150 38 517 231 396 5964
Fragments arranged by size:
5964 517 396 388 231 188 150 146
107 60 38 1 1 1
EcoRI G'AATT_C
Cuts at: 5497 5875 5497
Size: 378 7810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 4750 5398 2617
Size: 2133 648 5407
Fragments arranged by size:
5407 2133 648
FspI TGC'GCA
Cuts at: 2206 6694 2206
Size: 4488 3700
HaeII r_GCGC'y
Cuts at: 1678 2924 3764 5354 7335 1678
Size: 1246 840 1590 1981 2531
Fragments arranged by size:
2531 1981 1590 1246 840
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 3757 3789 4206
Size: 32 135 32 555 32 1598 32 417
Cuts at: 4206 4238 4949 4981 5078 5110 6829 6861 6903
Size: 32 711 32 97 32 1719 32 42
Cuts at: 6903 6935 1373
Size: 32 2626
Fragments arranged by size:
2626 1719 1598 711 555 417 135 97
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4591 5532 5778 669
Size: 988 281 2653 941 246 3079
Fragments arranged by size:
3079 2653 988 941 281 246
HindIII A'AGCT_T
Cuts at: 748 5375 748
Size: 4627 3561
HpaI GTT'AAC
Cuts at: 5778 5778
Size: 8188
KpnI G_GTAC'C
Cuts at: 5513 5513
Size: 8188
MfeI C'AATT_G
Cuts at: 5787 5787
Size: 8188
MluI A'CGCG_T
Cuts at: 8064 8064
Size: 8188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2686 7302 7486 1305
Size: 1381 4616 184 2007
Fragments arranged by size:
4616 2007 1381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3620 3872 3899 4892 10
Size: 54 1425 343 1788 252 27 993 3306
Fragments arranged by size:
3306 1788 1425 993 343 252 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2730 3176 3314 3450 4518 5219
Size: 648 1053 511 446 138 136 1068 701
Cuts at: 5219 5315 6146 6505 6664 518
Size: 96 831 359 159 2042
Fragments arranged by size:
2042 1068 1053 831 701 648 511 446
359 159 138 136 96
MspA1I CmG'CkG
Cuts at: 1606 2210 2239 2986 3712 4581 4741 5082 5460
Size: 604 29 747 726 869 160 341 378
Cuts at: 5460 5604 6281 7429 7674 1606
Size: 144 677 1148 245 2120
Fragments arranged by size:
2120 1148 869 747 726 677 604 378
341 245 160 144 29
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7636
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8188
NheI G'CTAG_C
Cuts at: 1052 3232 1052
Size: 2180 6008
NotI GC'GGCC_GC
Cuts at: 5635 5635
Size: 8188
NspI r_CATG'y
Cuts at: 1132 1229 2224 2269 3181 4539 5546 7091 1132
Size: 97 995 45 912 1358 1007 1545 2229
Fragments arranged by size:
2229 1545 1358 1007 995 912 97 45
PasI CC'CwG_GG
Cuts at: 1348 3552 4111 4663 1348
Size: 2204 559 552 4873
Fragments arranged by size:
4873 2204 559 552
PciI A'CATG_T
Cuts at: 2265 3177 7087 2265
Size: 912 3910 3366
Fragments arranged by size:
3910 3366 912
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3327 1108
Size: 2219 5969
PfoI T'CCnGG_A
Cuts at: 2088 5106 5236 2088
Size: 3018 130 5040
Fragments arranged by size:
5040 3018 130
PmeI GTTT'AAAC
Cuts at: 5492 5492
Size: 8188
PmlI CAC'GTG
Cuts at: 2938 2938
Size: 8188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2377 6257 6289 7770 7802 2345
Size: 32 3880 32 1481 32 2731
Fragments arranged by size:
3880 2731 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2882 3778 4333 2882
Size: 896 555 6737
Fragments arranged by size:
6737 896 555
PshAI GACnn'nnGTC
Cuts at: 1613 4044 4489 1613
Size: 2431 445 5312
Fragments arranged by size:
5312 2431 445
PsiI TTA'TAA
Cuts at: 5758 5758
Size: 8188
PspOMI G'GGCC_C
Cuts at: 2736 3270 3438 3627 3816 2736
Size: 534 168 189 189 7108
Fragments arranged by size:
7108 534 189 189 168
PstI C_TGCA'G
Cuts at: 830 2991 3747 4368 4586 5076 5540 830
Size: 2161 756 621 218 490 464 3478
Fragments arranged by size:
3478 2161 756 621 490 464 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8188
PvuII CAG'CTG
Cuts at: 2210 2986 4741 5460 2210
Size: 776 1755 719 4938
Fragments arranged by size:
4938 1755 776 719
SacI G_AGCT'C
Cuts at: 4388 4398 4749 5263 4388
Size: 10 351 514 7313
Fragments arranged by size:
7313 514 351 10
SalI G'TCGA_C
Cuts at: 1936 5530 1936
Size: 3594 4594
SanDI GG'GwC_CC
Cuts at: 2882 2882
Size: 8188
SbfI CC_TGCA'GG
Cuts at: 5540 5540
Size: 8188
ScaI AGT'ACT
Cuts at: 2579 4805 6436 8148 2579
Size: 2226 1631 1712 2619
Fragments arranged by size:
2619 2226 1712 1631
SexAI A'CCwGG_T
Cuts at: 3258 3258
Size: 8188
SfcI C'TryA_G
Cuts at: 826 1045 2987 3743 4364 4582 5072 5536 6671
Size: 219 1942 756 621 218 490 464 1135
Cuts at: 6671 7352 7543 826
Size: 681 191 1471
Fragments arranged by size:
1942 1471 1135 756 681 621 490 464
219 218 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3626 3815 3626
Size: 189 7999
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8188
SmaI CCC'GGG
Cuts at: 1872 3633 3821 5515 1872
Size: 1761 188 1694 4545
Fragments arranged by size:
4545 1761 1694 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6292 7193 7455 820
Size: 197 926 4349 901 262 1553
Fragments arranged by size:
4349 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5979 493
Size: 5486 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8188
SphI G_CATG'C
Cuts at: 1132 5546 1132
Size: 4414 3774
SspI AAT'ATT
Cuts at: 5 52 6112 5
Size: 47 6060 2081
Fragments arranged by size:
6060 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 4362 1517
Size: 1156 1689 5343
Fragments arranged by size:
5343 1689 1156
StyI C'CwwG_G
Cuts at: 513 1065 2271 2965 3494 3900 5627 513
Size: 552 1206 694 529 406 1727 3074
Fragments arranged by size:
3074 1727 1206 694 552 529 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2627 3326 3800 4328 6215 6232 6385
Size: 1255 1047 699 474 528 1887 17 153
Cuts at: 6385 6570 7015 325
Size: 185 445 1498
Fragments arranged by size:
1887 1498 1255 1047 699 528 474 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 2787 4803
Size: 274 80 33 51 1443 600 210 2016
Cuts at: 4803 5223 5370 6434 8146 96
Size: 420 147 1064 1712 138
Fragments arranged by size:
2016 1712 1443 1064 600 420 274 210
147 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 4114 4146 4411 4443 8148 8180 3038
Size: 32 1044 32 265 32 3705 32 3046
Fragments arranged by size:
3705 3046 1044 265 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2456 5291 1316
Size: 1140 2835 4213
Fragments arranged by size:
4213 2835 1140
XbaI T'CTAG_A
Cuts at: 5524 5524
Size: 8188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4738 4738
Size: 8188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8188
XmaI C'CCGG_G
Cuts at: 1870 3631 3819 5513 1870
Size: 1761 188 1694 4545
Fragments arranged by size:
4545 1761 1694 188
XmnI GAAnn'nnTTC
Cuts at: 6317 6317
Size: 8188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5089 5996 276
Size: 53 83 186 936 3555 907 2468
Fragments arranged by size:
3555 2468 936 907 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SfoI SrfI SwaI