Restriction map for
pFN21AB3327
(Circular) (Six-base) MAPSORT of: pFN21AB3327.seq Check: 462 from: 1 to: 7390
pFN21AB3327 7390 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2838 3704 2838
Size: 866 6524
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5200 278
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4616 4733 1937
Size: 2679 117 4594
Fragments arranged by size:
4594 2679 117
Acc65I G'GTAC_C
Cuts at: 2042 4711 2042
Size: 2669 4721
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 4578 5453 6837 7105 1632
Size: 861 2085 875 1384 268 1917
Fragments arranged by size:
2085 1917 1384 875 861 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7193
AflIII A'CryG_T
Cuts at: 1203 2839 3101 6289 7266 1203
Size: 1636 262 3188 977 1327
Fragments arranged by size:
3188 1636 1327 977 262
AgeI A'CCGG_T
Cuts at: 3263 6221 3263
Size: 2958 4432
AhdI GACnn_n'nnGTC
Cuts at: 3546 3683 6119 3546
Size: 137 2436 4817
Fragments arranged by size:
4817 2436 137
AleI CACnn'nnGTG
Cuts at: 1166 2580 2802 1166
Size: 1414 222 5754
Fragments arranged by size:
5754 1414 222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4553 1184
Size: 3369 4021
AlwNI CAG_nnn'CTG
Cuts at: 3143 4297 5133 6705 3143
Size: 1154 836 1572 3828
Fragments arranged by size:
3828 1572 1154 836
ApaLI G'TGCA_C
Cuts at: 5447 6603 5447
Size: 1156 6234
ApoI r'AATT_y
Cuts at: 1493 2931 3285 3807 3949 4699 4925 5077 7186
Size: 1438 354 522 142 750 226 152 2109
Cuts at: 7186 1493
Size: 1697
Fragments arranged by size:
2109 1697 1438 750 522 354 226 152
142
AseI AT'TA_AT
Cuts at: 160 1032 5944 160
Size: 872 4912 1606
Fragments arranged by size:
4912 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 4715 1870
Size: 73 2772 4545
Fragments arranged by size:
4545 2772 73
AvrII C'CTAG_G
Cuts at: 2491 2491
Size: 7390
BamHI G'GATC_C
Cuts at: 2404 3874 4720 5087 2404
Size: 1470 846 367 4707
Fragments arranged by size:
4707 1470 846 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2756 3388 3739 3962
Size: 325 217 627 255 714 632 351 223
Cuts at: 3962 4537 4711 6166 618
Size: 575 174 1455 1842
Fragments arranged by size:
1842 1455 714 632 627 575 351 325
255 223 217 174
BanII G_rGCy'C
Cuts at: 1391 2274 3959 4709 1391
Size: 883 1685 750 4072
Fragments arranged by size:
4072 1685 883 750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 3266 3330 3542 4083 4392 928
Size: 971 266 1101 64 212 541 309 3926
Fragments arranged by size:
3926 1101 971 541 309 266 212 64
BbvCI CC'TCA_GC
Cuts at: 3763 3763
Size: 7390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5581 5615 1903
Size: 34 3644 34 3678
Fragments arranged by size:
3678 3644 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5282 6492 5282
Size: 1210 6180
BclI T'GATC_A
Cuts at: 1548 2794 2962 1548
Size: 1246 168 5976
Fragments arranged by size:
5976 1246 168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2496 6001 136
Size: 107 122 71 631 605 824 3505 1525
Fragments arranged by size:
3505 1525 824 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7385 7385
Size: 7390
BlpI GC'TnA_GC
Cuts at: 2268 4807 2268
Size: 2539 4851
Bme1580I G_kGCm'C
Cuts at: 1790 5451 6607 1790
Size: 3661 1156 2573
Fragments arranged by size:
3661 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5121 6079 442
Size: 416 53 83 399 24 3704 958 1753
Fragments arranged by size:
3704 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4440 1056
Size: 3384 4006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 2487 6050 1239
Size: 1230 18 3563 2579
Fragments arranged by size:
3563 2579 1230 18
Bpu10I CC'TnA_GC
Cuts at: 3763 3763
Size: 7390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5515 6380 6678 5515
Size: 865 298 6227
Fragments arranged by size:
6227 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2487 3533 3691 6053 882
Size: 617 988 1046 158 2362 2219
Fragments arranged by size:
2362 2219 1046 988 617 158
BsaAI yAC'GTr
Cuts at: 493 1204 5181 493
Size: 711 3977 2702
Fragments arranged by size:
3977 2702 711
BsaBI GATnn'nnATC
Cuts at: 7384 7384
Size: 7390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2321 2520 5197 5579
Size: 53 83 186 936 788 199 2677 382
Cuts at: 5579 6214 275
Size: 635 1451
Fragments arranged by size:
2677 1451 936 788 635 382 199 186
83 53
BsaWI w'CCGG_w
Cuts at: 3263 5823 6221 6495 6642 7053 3263
Size: 2560 398 274 147 411 3600
Fragments arranged by size:
3600 2560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 3671 3701 4129
Size: 30 165 30 1002 30 1236 30 428
Cuts at: 4129 4159 1178
Size: 30 4409
Fragments arranged by size:
4409 1236 1002 428 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2426 2648 3751 1186
Size: 1047 193 222 1103 4825
Fragments arranged by size:
4825 1103 1047 222 193
BseYI C'CCAG_C
Cuts at: 2571 6593 7089 2571
Size: 4022 496 2872
Fragments arranged by size:
4022 2872 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2896 2989 3156 1163
Size: 573 1160 93 167 5397
Fragments arranged by size:
5397 1160 573 167 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2201 2214 2265 4840 5601 6629
Size: 275 186 200 13 51 2575 761 1028
Cuts at: 6629 1540
Size: 2301
Fragments arranged by size:
2575 2301 1028 761 275 200 186 51
13
BsiHKAI G_wGCw'C
Cuts at: 5451 5536 6607 5451
Size: 85 1071 6234
Fragments arranged by size:
6234 1071 85
BsmI GAATG_Cn'
Cuts at: 4901 4994 4901
Size: 93 7297
BsmBI CGTCTCn'nnnn_
Cuts at: 4149 4149
Size: 7390
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 3959 4709 5451 5536 6607 1391
Size: 399 484 1685 750 742 85 1071 2174
Fragments arranged by size:
2174 1685 1071 750 742 484 399 85
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 3793 5279 7009 3793
Size: 1486 1730 4174
Fragments arranged by size:
4174 1730 1486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3704 4745 844
Size: 1994 866 1041 3489
Fragments arranged by size:
3489 1994 1041 866
BsrBI CCG'CTC
Cuts at: 1394 1823 5277 1394
Size: 429 3454 3507
Fragments arranged by size:
3507 3454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2234 4196 5885 6059 65
Size: 1183 986 1962 1689 174 1396
Fragments arranged by size:
1962 1689 1396 1183 986 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2200 2314 3263 6034 6221 1700
Size: 110 235 155 114 949 2771 187 2869
Fragments arranged by size:
2869 2771 949 235 187 155 114 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7390
BssSI C'ACGA_G
Cuts at: 3897 5450 6462 3897
Size: 1553 1012 4825
Fragments arranged by size:
4825 1553 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7390
BstBI TT'CG_AA
Cuts at: 6194 6194
Size: 7390
BstEII G'GTnAC_C
Cuts at: 1184 2182 1184
Size: 998 6392
BstXI CCAn_nnnn'nTGG
Cuts at: 2450 2450
Size: 7390
BstYI r'GATC_y
Cuts at: 1972 2404 3874 4229 4447 4720 5087 5472 5489
Size: 432 1470 355 218 273 367 385 17
Cuts at: 5489 6941 7027 7039 7385 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1470 1452 432 385 367 355 346
273 218 86 17 12
BstZ17I GTA'TAC
Cuts at: 4617 4617
Size: 7390
Bsu36I CC'TnA_GG
Cuts at: 2409 3916 2409
Size: 1507 5883
BtgI C'CryG_G
Cuts at: 513 1065 2328 2400 2623 513
Size: 552 1263 72 223 5280
Fragments arranged by size:
5280 1263 552 223 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6651
Fragments arranged by size:
6651 683 56
BtsI GCAGTG_nn'
Cuts at: 2023 4906 5700 5720 2023
Size: 2883 794 20 3693
Fragments arranged by size:
3693 2883 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7355
DraI TTT'AAA
Cuts at: 4694 4694
Size: 7390
DraIII CAC_nnn'GTG
Cuts at: 3023 3023
Size: 7390
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2654 4597 6397 809
Size: 507 1338 1943 1800 1802
Fragments arranged by size:
1943 1802 1800 1338 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2211 2262
Size: 54 1425 50 275 18 368 13 51
Cuts at: 2262 4165 4837 5726 7355 8
Size: 1903 672 889 1629 43
Fragments arranged by size:
1903 1629 1425 889 672 368 275 54
51 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2198 2211 2262 4837 1537
Size: 275 386 13 51 2575 4090
Fragments arranged by size:
4090 2575 386 275 51 13
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3377 5320 1353
Size: 1116 908 1943 3423
Fragments arranged by size:
3423 1943 1116 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2052 2271 5977 6351 6497 387
Size: 1064 138 463 219 3706 374 146 1280
Fragments arranged by size:
3706 1280 1064 463 374 219 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2487 2493 4578 5453 6050 6837
Size: 393 837 18 6 2085 875 597 787
Cuts at: 6837 7105 1239
Size: 268 1524
Fragments arranged by size:
2085 1524 875 837 787 597 393 268
18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2344 4022 2344
Size: 1678 5712
EcoO109I rG'GnC_Cy
Cuts at: 2349 2530 2349
Size: 181 7209
EcoRI G'AATT_C
Cuts at: 3807 4699 5077 3807
Size: 892 378 6120
Fragments arranged by size:
6120 892 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 7390
FspI TGC'GCA
Cuts at: 5896 5896
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 6537 1678
Size: 4859 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 2829 2861 3532
Size: 32 135 32 833 32 392 32 671
Cuts at: 3532 3564 3669 3701 3830 3862 4300 4322 4332
Size: 32 105 32 129 32 438 22 10
Cuts at: 4332 4354 4408 4435 4440 4467 6031 6063 6105
Size: 22 54 27 5 27 1564 32 42
Cuts at: 6105 6137 1373
Size: 32 2626
Fragments arranged by size:
2626 1564 833 671 438 392 135 129
105 54 42 32 32 32 32 32
32 32 32 32 27 27 22 22
10 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4281 4734 4980 669
Size: 988 281 2343 453 246 3079
Fragments arranged by size:
3079 2343 988 453 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7390
HpaI GTT'AAC
Cuts at: 4980 4980
Size: 7390
KpnI G_GTAC'C
Cuts at: 2046 4715 2046
Size: 2669 4721
MfeI C'AATT_G
Cuts at: 4989 4989
Size: 7390
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2722 3873 4045 6504 6688 1305
Size: 1417 1151 172 2459 184 2007
Fragments arranged by size:
2459 2007 1417 1151 184 172
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5568
Fragments arranged by size:
5568 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2448 2550 2580 2790 2802 2956 3040
Size: 648 1282 102 30 210 12 154 84
Cuts at: 3040 5348 5707 5866 518
Size: 2308 359 159 2042
Fragments arranged by size:
2308 2042 1282 648 359 210 159 154
102 84 30 12
MspA1I CmG'CkG
Cuts at: 1606 2112 2267 2330 2402 4806 5483 6631 6876
Size: 506 155 63 72 2404 677 1148 245
Cuts at: 6876 1606
Size: 2120
Fragments arranged by size:
2404 2120 1148 677 506 245 155 72
63
NaeI GCC'GGC
Cuts at: 2202 2316 2202
Size: 114 7276
NcoI C'CATG_G
Cuts at: 513 1065 2623 513
Size: 552 1558 5280
Fragments arranged by size:
5280 1558 552
NdeI CA'TA_TG
Cuts at: 387 2997 387
Size: 2610 4780
NgoMIV G'CCGG_C
Cuts at: 2200 2314 2200
Size: 114 7276
NheI G'CTAG_C
Cuts at: 1052 4436 1052
Size: 3384 4006
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NspI r_CATG'y
Cuts at: 1132 1229 2555 2843 3105 3307 4748 6293 1132
Size: 97 1326 288 262 202 1441 1545 2229
Fragments arranged by size:
2229 1545 1441 1326 288 262 202 97
PasI CC'CwG_GG
Cuts at: 1348 2146 1348
Size: 798 6592
PciI A'CATG_T
Cuts at: 2839 3101 6289 2839
Size: 262 3188 3940
Fragments arranged by size:
3940 3188 262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 4297 1108
Size: 1044 2145 4201
Fragments arranged by size:
4201 2145 1044
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 7390
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5459 5491 6972 7004 5459
Size: 32 1481 32 5845
Fragments arranged by size:
5845 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2349 2530 2349
Size: 181 7209
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7390
PsiI TTA'TAA
Cuts at: 4960 4960
Size: 7390
PstI C_TGCA'G
Cuts at: 830 3381 3715 4742 830
Size: 2551 334 1027 3478
Fragments arranged by size:
3478 2551 1027 334
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
SacII CC_GC'GG
Cuts at: 2331 2403 2331
Size: 72 7318
SalI G'TCGA_C
Cuts at: 1936 4732 1936
Size: 2796 4594
SbfI CC_TGCA'GG
Cuts at: 4742 4742
Size: 7390
ScaI AGT'ACT
Cuts at: 5638 7350 5638
Size: 1712 5678
SfcI C'TryA_G
Cuts at: 826 1045 2800 2915 3377 3711 4738 5873 6554
Size: 219 1755 115 462 334 1027 1135 681
Cuts at: 6554 6745 826
Size: 191 1471
Fragments arranged by size:
1755 1471 1135 1027 681 462 334 219
191 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7390
SmaI CCC'GGG
Cuts at: 1872 4717 1872
Size: 2845 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5494 6395 6657 820
Size: 197 926 3551 901 262 1553
Fragments arranged by size:
3551 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5181 493
Size: 4688 2702
SpeI A'CTAG_T
Cuts at: 152 3131 152
Size: 2979 4411
SphI G_CATG'C
Cuts at: 1132 4748 1132
Size: 3616 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5314 5
Size: 47 3834 1428 2081
Fragments arranged by size:
3834 2081 1428 47
StuI AGG'CCT
Cuts at: 1517 2490 1517
Size: 973 6417
StyI C'CwwG_G
Cuts at: 513 1065 2491 2623 4829 513
Size: 552 1426 132 2206 3074
Fragments arranged by size:
3074 2206 1426 552 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3980 4684 5417 5434 5587 5772 6217
Size: 1255 2400 704 733 17 153 185 445
Cuts at: 6217 325
Size: 1498
Fragments arranged by size:
2400 1498 1255 733 704 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3560 4652 5636
Size: 274 80 33 51 1443 1583 1092 984
Cuts at: 5636 7348 96
Size: 1712 138
Fragments arranged by size:
1712 1583 1443 1092 984 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 3007 3039 3671 3703 4102 4129 4134
Size: 32 274 32 632 32 399 27 5
Cuts at: 4134 4161 7350 7382 2701
Size: 27 3189 32 2709
Fragments arranged by size:
3189 2709 632 399 274 32 32 32
32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7390
XbaI T'CTAG_A
Cuts at: 4726 4726
Size: 7390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2447 2734 3563 2447
Size: 287 829 6274
Fragments arranged by size:
6274 829 287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7390
XmaI C'CCGG_G
Cuts at: 1870 4715 1870
Size: 2845 4545
XmnI GAAnn'nnTTC
Cuts at: 3936 5519 3936
Size: 1583 5807
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5198 276
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI