Restriction map for
pFN21AB3324
(Circular) (Six-base) MAPSORT of: pFN21AB3324.seq Check: 2010 from: 1 to: 6520
pFN21AB3324 6520 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3300 3646 3863 1937
Size: 1363 346 217 4594
Fragments arranged by size:
4594 1363 346 217
Acc65I G'GTAC_C
Cuts at: 2649 3841 2649
Size: 1192 5328
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4583 5967 6235 1632
Size: 2951 1384 268 1917
Fragments arranged by size:
2951 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 3135 5419 6396 1203
Size: 1932 2284 977 1327
Fragments arranged by size:
2284 1932 1327 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 3642 5249 3642
Size: 1607 4913
AleI CACnn'nnGTG
Cuts at: 1166 3150 1166
Size: 1984 4536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 2138 2262 4263 5835 2138
Size: 124 2001 1572 2823
Fragments arranged by size:
2823 2001 1572 124
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2161 2224 2456 2889 3015 3374 3698 3829
Size: 668 63 232 433 126 359 324 131
Cuts at: 3829 4055 4207 6316 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 668 433 359 324 232 226
152 131 126 63
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2562 3845 1870
Size: 73 619 1283 4545
Fragments arranged by size:
4545 1283 619 73
AvrII C'CTAG_G
Cuts at: 2201 2201
Size: 6520
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2649 3841 5296 618
Size: 325 217 627 862 1192 1455 1842
Fragments arranged by size:
1842 1455 1192 862 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3839 1391
Size: 2448 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 2312 2878 928
Size: 971 285 128 566 4570
Fragments arranged by size:
4570 971 566 285 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 2941 3069 1548
Size: 1393 128 4999
Fragments arranged by size:
4999 1393 128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5131 136
Size: 107 122 71 631 605 3459 1525
Fragments arranged by size:
3459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2982 6515 2982
Size: 3533 2987
BlpI GC'TnA_GC
Cuts at: 3937 3937
Size: 6520
Bme1580I G_kGCm'C
Cuts at: 1790 2367 2505 4581 5737 1790
Size: 577 138 2076 1156 2573
Fragments arranged by size:
2573 2076 1156 577 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4251 5209 442
Size: 416 53 83 399 24 2834 958 1753
Fragments arranged by size:
2834 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 3035 3799 5180 1239
Size: 832 964 764 1381 2579
Fragments arranged by size:
2579 1381 964 832 764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 4645 5510 5808 2027
Size: 2618 865 298 2739
Fragments arranged by size:
2739 2618 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2073 5183 882
Size: 617 574 3110 2219
Fragments arranged by size:
3110 2219 617 574
BsaAI yAC'GTr
Cuts at: 493 1204 4311 493
Size: 711 3107 2702
Fragments arranged by size:
3107 2702 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4327 4709 5344 275
Size: 53 83 186 936 2794 382 635 1451
Fragments arranged by size:
2794 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3258 3668 4953 5351 5625 5772 6183 3258
Size: 410 1285 398 274 147 411 3595
Fragments arranged by size:
3595 1285 411 410 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6295
Fragments arranged by size:
6295 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2781 1186
Size: 1595 4925
BseYI C'CCAG_C
Cuts at: 2077 5723 6219 2077
Size: 3646 496 2378
Fragments arranged by size:
3646 2378 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3149 1163
Size: 573 1413 4534
Fragments arranged by size:
4534 1413 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3970 4731 5759 1540
Size: 275 186 15 1954 761 1028 2301
Fragments arranged by size:
2301 1954 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2057 2815 3628 4581 4666 5737 2057
Size: 758 813 953 85 1071 2840
Fragments arranged by size:
2840 1071 953 813 758 85
BsmI GAATG_Cn'
Cuts at: 2791 3011 3664 4031 4124 2791
Size: 220 653 367 93 5187
Fragments arranged by size:
5187 653 367 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2367 2505 2815 3628 3839 4581
Size: 399 267 310 138 310 813 211 742
Cuts at: 4581 4666 5737 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 813 742 399 310 310 267
211 138 85
BspEI T'CCGG_A
Cuts at: 3258 3668 6183 3258
Size: 410 2515 3595
Fragments arranged by size:
3595 2515 410
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3206 3875 844
Size: 2362 669 3489
Fragments arranged by size:
3489 2362 669
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3216 3408 5015 5189 65
Size: 1183 1968 192 1607 174 1396
Fragments arranged by size:
1968 1607 1396 1183 192 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5164 5351 1700
Size: 110 3354 187 2869
Fragments arranged by size:
3354 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2297 794
Size: 1503 5017
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 3193 1184
Size: 2009 4511
BstYI r'GATC_y
Cuts at: 1972 2982 3598 3850 4217 4602 4619 6071 6157
Size: 1010 616 252 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1010 616 385 367 346 252
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3093 513
Size: 552 939 1089 3940
Fragments arranged by size:
3940 1089 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 487
Size: 683 56 905 4876
Fragments arranged by size:
4876 905 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 2438 2893 4036 4830 4850 2295
Size: 143 455 1143 794 20 3965
Fragments arranged by size:
3965 1143 794 455 143 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DraIII CAC_nnn'GTG
Cuts at: 2621 2621
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2322 3967
Size: 54 1425 50 275 18 183 309 1645
Cuts at: 3967 4856 6485 8
Size: 889 1629 43
Fragments arranged by size:
1645 1629 1425 889 309 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3967 1537
Size: 275 201 1954 4090
Fragments arranged by size:
4090 1954 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2734 3667 4450 1353
Size: 1381 933 783 3423
Fragments arranged by size:
3423 1381 933 783
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 5107 5481 5627 387
Size: 1064 138 522 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 522 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 3035 3799 4583 5180 5967 6235
Size: 393 439 964 764 784 597 787 268
Cuts at: 6235 1239
Size: 1524
Fragments arranged by size:
1524 964 787 784 764 597 439 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3384 3570 3384
Size: 186 6334
EcoRI G'AATT_C
Cuts at: 2161 2224 2889 3015 3698 3829 4207 2161
Size: 63 665 126 683 131 378 4474
Fragments arranged by size:
4474 683 665 378 131 126 63
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 5667 1678
Size: 3989 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 2597 2629 2698
Size: 32 135 32 848 32 145 32 69
Cuts at: 2698 2730 3221 3253 3273 3305 3628 3660 5161
Size: 32 491 32 20 32 323 32 1501
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1501 848 491 323 145 135 69
42 32 32 32 32 32 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3864 4110 669
Size: 988 281 1926 246 3079
Fragments arranged by size:
3079 1926 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2684 748
Size: 1936 4584
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 2653 3845 2653
Size: 1192 5328
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3253 5634 5818 1305
Size: 1948 2381 184 2007
Fragments arranged by size:
2381 2007 1948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 10
Size: 54 1425 343 492 4206
Fragments arranged by size:
4206 1425 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3004 3150 3191 4478 4837 4996
Size: 648 1134 704 146 41 1287 359 159
Cuts at: 4996 518
Size: 2042
Fragments arranged by size:
2042 1287 1134 704 648 359 159 146
41
MspA1I CmG'CkG
Cuts at: 1606 2250 3764 3936 4613 5761 6006 1606
Size: 644 1514 172 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 644 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5029
Fragments arranged by size:
5029 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2107 2674 3498 3878 5423 1132
Size: 97 878 567 824 380 1545 2229
Fragments arranged by size:
2229 1545 878 824 567 380 97
PasI CC'CwG_GG
Cuts at: 1348 2132 1348
Size: 784 5736
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2138 1108
Size: 1030 5490
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3384 3384
Size: 6520
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PstI C_TGCA'G
Cuts at: 830 3634 3872 830
Size: 2804 238 3478
Fragments arranged by size:
3478 2804 238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2250 3764 2250
Size: 1514 5006
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SbfI CC_TGCA'GG
Cuts at: 3634 3872 3634
Size: 238 6282
ScaI AGT'ACT
Cuts at: 3367 4768 6480 3367
Size: 1401 1712 3407
Fragments arranged by size:
3407 1712 1401
SfcI C'TryA_G
Cuts at: 826 1045 3630 3686 3868 5003 5684 5875 826
Size: 219 2585 56 182 1135 681 191 1471
Fragments arranged by size:
2585 1471 1135 681 219 191 182 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3847 1872
Size: 1975 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 4624 5525 5787 820
Size: 197 926 99 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 262 197 99
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 2107 2674 3878 1132
Size: 975 567 1204 3774
Fragments arranged by size:
3774 1204 975 567
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 2727 1517
Size: 1210 5310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2201 3959 513
Size: 552 939 197 1758 3074
Fragments arranged by size:
3074 1758 939 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4547 4564 4717 4902 5347 325
Size: 1255 2967 17 153 185 445 1498
Fragments arranged by size:
2967 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3315 3365 3423
Size: 274 80 33 51 1443 1338 50 58
Cuts at: 3423 4766 6478 96
Size: 1343 1712 138
Fragments arranged by size:
1712 1443 1343 1338 274 138 80 58
51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3550 3582 6480 6512 3550
Size: 32 2898 32 3558
Fragments arranged by size:
3558 2898 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3845 1870
Size: 1975 4545
XmnI GAAnn'nnTTC
Cuts at: 3159 4649 3159
Size: 1490 5030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI