Restriction map for
pFN21AB3316
(Circular) (Six-base) MAPSORT of: pFN21AB3316.seq Check: 8086 from: 1 to: 6922
pFN21AB3316 6922 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3319 3987 4265 1937
Size: 1382 668 278 4594
Fragments arranged by size:
4594 1382 668 278
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2442 2904 3078 4985 6369 6637 1632
Size: 519 291 462 174 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 519 462 291 268 174
AfeI AGC'GCT
Cuts at: 2068 2068
Size: 6922
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 5821 6798 1203
Size: 4618 977 1327
Fragments arranged by size:
4618 1327 977
AgeI A'CCGG_T
Cuts at: 3839 5753 3839
Size: 1914 5008
AhdI GACnn_n'nnGTC
Cuts at: 2758 3967 5651 2758
Size: 1209 1684 4029
Fragments arranged by size:
4029 1684 1209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2310 1184
Size: 1126 5796
AlwNI CAG_nnn'CTG
Cuts at: 2276 4003 4665 6237 2276
Size: 1727 662 1572 2961
Fragments arranged by size:
2961 1727 1572 662
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 2516 3021 3396 3513 3527 3675 3748 3911
Size: 1023 505 375 117 14 148 73 163
Cuts at: 3911 3937 4231 4457 4609 6718 1493
Size: 26 294 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 505 375 294 226 163
152 148 117 73 26 14
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 1870
Size: 73 2304 4545
Fragments arranged by size:
4545 2304 73
BamHI G'GATC_C
Cuts at: 4252 4619 4252
Size: 367 6555
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4103 4243 5698 618
Size: 325 217 627 2316 140 1455 1842
Fragments arranged by size:
2316 1842 1455 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2057 4241 1391
Size: 666 2184 4072
Fragments arranged by size:
4072 2184 666
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3713 928
Size: 971 1814 4137
Fragments arranged by size:
4137 1814 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5113 5147 1903
Size: 34 50 34 3092 34 3678
Fragments arranged by size:
3678 3092 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4814 6024 4814
Size: 1210 5712
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5533 136
Size: 107 122 71 631 605 3861 1525
Fragments arranged by size:
3861 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6917 6917
Size: 6922
BlpI GC'TnA_GC
Cuts at: 4182 4339 4182
Size: 157 6765
Bme1580I G_kGCm'C
Cuts at: 1790 4983 6139 1790
Size: 3193 1156 2573
Fragments arranged by size:
3193 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 4054 4653
Size: 416 53 83 399 24 780 1857 599
Cuts at: 4653 5611 442
Size: 958 1753
Fragments arranged by size:
1857 1753 958 780 599 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2697 5582 1239
Size: 1458 2885 2579
Fragments arranged by size:
2885 2579 1458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5047 5912 6210 5047
Size: 865 298 5759
Fragments arranged by size:
5759 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2328 3369 5585 882
Size: 617 829 1041 2216 2219
Fragments arranged by size:
2219 2216 1041 829 617
BsaAI yAC'GTr
Cuts at: 493 1204 4713 493
Size: 711 3509 2702
Fragments arranged by size:
3509 2702 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4729 5111 5746 275
Size: 53 83 186 936 3196 382 635 1451
Fragments arranged by size:
3196 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3661 3839 5355 5753 6027 6174 6585 3661
Size: 178 1516 398 274 147 411 3998
Fragments arranged by size:
3998 1516 411 398 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 3799 3829 1178
Size: 30 165 30 639 30 1727 30 4271
Fragments arranged by size:
4271 1727 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2126 2475 1186
Size: 937 3 349 5633
Fragments arranged by size:
5633 937 349 3
BseYI C'CCAG_C
Cuts at: 6125 6621 6125
Size: 496 6426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2961 1163
Size: 573 1225 5124
Fragments arranged by size:
5124 1225 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4983 5068 6139 4023
Size: 960 85 1071 4806
Fragments arranged by size:
4806 1071 960 85
BsmI GAATG_Cn'
Cuts at: 2868 2910 3117 4433 4526 2868
Size: 42 207 1316 93 5264
Fragments arranged by size:
5264 1316 207 93 42
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 4023 4241 4983 5068 6139 1391
Size: 399 267 1966 218 742 85 1071 2174
Fragments arranged by size:
2174 1966 1071 742 399 267 218 85
BspEI T'CCGG_A
Cuts at: 3661 6585 3661
Size: 2924 3998
BspHI T'CATG_A
Cuts at: 3726 4811 6541 3726
Size: 1085 1730 4107
Fragments arranged by size:
4107 1730 1085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3049 4277 844
Size: 2205 1228 3489
Fragments arranged by size:
3489 2205 1228
BsrBI CCG'CTC
Cuts at: 1394 1823 3710 4170 4809 1394
Size: 429 1887 460 639 3507
Fragments arranged by size:
3507 1887 639 460 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5417 5591 65
Size: 1183 4169 174 1396
Fragments arranged by size:
4169 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3839 5566 5753 1700
Size: 110 2029 1727 187 2869
Fragments arranged by size:
2869 2029 1727 187 110
BsrGI T'GTAC_A
Cuts at: 96 3214 96
Size: 3118 3804
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 4982 5994 4982
Size: 1012 5910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6922
BstBI TT'CG_AA
Cuts at: 3673 5726 3673
Size: 2053 4869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6922
BstYI r'GATC_y
Cuts at: 1972 2194 4252 4619 5004 5021 6473 6559 6571
Size: 222 2058 367 385 17 1452 86 12
Cuts at: 6571 6917 1972
Size: 346 1977
Fragments arranged by size:
2058 1977 1452 385 367 346 222 86
17 12
BstZ17I GTA'TAC
Cuts at: 3320 3320
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 2030 4107 513
Size: 552 965 2077 3328
Fragments arranged by size:
3328 2077 965 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6183
Fragments arranged by size:
6183 683 56
BtsI GCAGTG_nn'
Cuts at: 3354 3741 4438 5232 5252 3354
Size: 387 697 794 20 5024
Fragments arranged by size:
5024 794 697 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2856 2891 529
Size: 35 2292 35 4560
Fragments arranged by size:
4560 2292 35 35
DraI TTT'AAA
Cuts at: 2217 3037 3771 4226 2217
Size: 820 734 455 4913
Fragments arranged by size:
4913 820 734 455
DraIII CAC_nnn'GTG
Cuts at: 3128 3923 3128
Size: 795 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4369 5258 6887
Size: 54 1425 50 275 18 2539 889 1629
Cuts at: 6887 8
Size: 43
Fragments arranged by size:
2539 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 4852 1353
Size: 1657 1842 3423
Fragments arranged by size:
3423 1842 1657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 3018 5509 5883 6029 387
Size: 1064 138 450 979 2491 374 146 1280
Fragments arranged by size:
2491 1280 1064 979 450 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2442 2697 2904 3078 4985 5582
Size: 393 519 291 255 207 174 1907 597
Cuts at: 5582 6369 6637 1239
Size: 787 268 1524
Fragments arranged by size:
1907 1524 787 597 519 393 291 268
255 207 174
EcoNI CCTnn'n_nnAGG
Cuts at: 2808 2808
Size: 6922
EcoO109I rG'GnC_Cy
Cuts at: 3961 3961
Size: 6922
EcoRI G'AATT_C
Cuts at: 3937 4231 4609 3937
Size: 294 378 6250
Fragments arranged by size:
6250 378 294
EcoRV GAT'ATC
Cuts at: 2365 2365
Size: 6922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2873 2269
Size: 604 6318
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 2070 6069 1678
Size: 392 3999 2531
Fragments arranged by size:
3999 2531 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2744 2776 3953
Size: 32 135 32 470 32 670 32 1177
Cuts at: 3953 3985 3997 4029 5563 5595 5637 5669 1373
Size: 32 12 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 1177 670 470 135 42 32
32 32 32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 4266 4512 669
Size: 988 281 693 1635 246 3079
Fragments arranged by size:
3079 1635 988 693 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6922
HpaI GTT'AAC
Cuts at: 2631 4512 2631
Size: 1881 5041
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 3222 4521 3222
Size: 1299 5623
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 6036 6220 1305
Size: 780 3951 184 2007
Fragments arranged by size:
3951 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5100
Fragments arranged by size:
5100 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4880 5239 5398 518
Size: 648 3714 359 159 2042
Fragments arranged by size:
3714 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2020 3703 4186 4338 5015 6163 6408
Size: 405 9 1683 483 152 677 1148 245
Cuts at: 6408 1606
Size: 2120
Fragments arranged by size:
2120 1683 1148 677 483 405 245 152
9
NcoI C'CATG_G
Cuts at: 513 1065 4107 513
Size: 552 3042 3328
Fragments arranged by size:
3328 3042 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 4280 5825 1132
Size: 97 3051 1545 2229
Fragments arranged by size:
3051 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 5821 5821
Size: 6922
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6922
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2325 2357 2480 2512 4991 5023 6504 6536 2325
Size: 32 123 32 2479 32 1481 32 2711
Fragments arranged by size:
2711 2479 1481 123 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3961 3961
Size: 6922
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PstI C_TGCA'G
Cuts at: 830 4274 830
Size: 3444 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 2020 3703 2020
Size: 1683 5239
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SexAI A'CCwGG_T
Cuts at: 3963 3963
Size: 6922
SfcI C'TryA_G
Cuts at: 826 1045 4270 5405 6086 6277 826
Size: 219 3225 1135 681 191 1471
Fragments arranged by size:
3225 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5026 5927 6189 820
Size: 197 926 3083 901 262 1553
Fragments arranged by size:
3083 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 4280 1132
Size: 3148 3774
SspI AAT'ATT
Cuts at: 5 52 3888 4846 5
Size: 47 3836 958 2081
Fragments arranged by size:
3836 2081 958 47
StuI AGG'CCT
Cuts at: 1517 3055 1517
Size: 1538 5384
StyI C'CwwG_G
Cuts at: 513 1065 2809 4031 4107 4361 513
Size: 552 1744 1222 76 254 3074
Fragments arranged by size:
3074 1744 1222 552 254 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2352 4949 4966 5119 5304 5749 325
Size: 1255 772 2597 17 153 185 445 1498
Fragments arranged by size:
2597 1498 1255 772 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3214 5168 6880
Size: 274 80 33 51 1443 1237 1954 1712
Cuts at: 6880 96
Size: 138
Fragments arranged by size:
1954 1712 1443 1237 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2790 2822 3450 3482 3799 3831 6882 6914 2790
Size: 32 628 32 317 32 3051 32 2798
Fragments arranged by size:
3051 2798 628 317 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 3399 3588 3669 5051 3399
Size: 189 81 1382 5270
Fragments arranged by size:
5270 1382 189 81
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI