Restriction map for
pFN21AB3313
(Circular) (Six-base) MAPSORT of: pFN21AB3313.seq Check: 8280 from: 1 to: 6364
pFN21AB3313 6364 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2303 4174 278
Size: 53 83 186 936 767 1871 2468
Fragments arranged by size:
2468 1871 936 767 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2421 2433 2442 2979 4427 5811 6079
Size: 572 217 12 9 537 1448 1384 268
Cuts at: 6079 1632
Size: 1917
Fragments arranged by size:
1917 1448 1384 572 537 268 217 12
9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 3081 3661 5263 6240 1203
Size: 1878 580 1602 977 1327
Fragments arranged by size:
1878 1602 1327 977 580
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 2299 5093 2299
Size: 2794 3570
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 4753
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AlwNI CAG_nnn'CTG
Cuts at: 2885 3181 3249 4107 5679 2885
Size: 296 68 858 1572 3570
Fragments arranged by size:
3570 1572 858 296 68
ApaI G_GGCC'C
Cuts at: 2938 3035 2938
Size: 97 6267
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 2138 3673 3899 4051 6160 1493
Size: 645 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 3689 1870
Size: 73 1746 4545
Fragments arranged by size:
4545 1746 73
AvrII C'CTAG_G
Cuts at: 3283 3283
Size: 6364
BamHI G'GATC_C
Cuts at: 3001 3054 3694 4061 3001
Size: 53 640 367 5304
Fragments arranged by size:
5304 640 367 53
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3685 5140 618
Size: 325 217 627 1898 1455 1842
Fragments arranged by size:
1898 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2494 2938 3035 3683 1391
Size: 1103 444 97 648 4072
Fragments arranged by size:
4072 1103 648 444 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 3275 928
Size: 971 540 836 4017
Fragments arranged by size:
4017 971 836 540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4555 4589 1903
Size: 34 2618 34 3678
Fragments arranged by size:
3678 2618 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4975 136
Size: 107 122 71 631 605 337 2966 1525
Fragments arranged by size:
2966 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2669 3529 3781 2669
Size: 860 252 5252
Fragments arranged by size:
5252 860 252
Bme1580I G_kGCm'C
Cuts at: 1790 2938 3035 4425 5581 1790
Size: 1148 97 1390 1156 2573
Fragments arranged by size:
2573 1390 1156 1148 97
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 4095 5053
Size: 416 53 83 399 24 1613 1065 958
Cuts at: 5053 442
Size: 1753
Fragments arranged by size:
1753 1613 1065 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3237 5024 1239
Size: 1998 1787 2579
Fragments arranged by size:
2579 1998 1787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4489 5354 5652 4489
Size: 865 298 5201
Fragments arranged by size:
5201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 5027 882
Size: 617 1338 2190 2219
Fragments arranged by size:
2219 2190 1338 617
BsaAI yAC'GTr
Cuts at: 493 1204 3076 4155 493
Size: 711 1872 1079 2702
Fragments arranged by size:
2702 1872 1079 711
BsaBI GATnn'nnATC
Cuts at: 2643 3317 6358 2643
Size: 674 3041 2649
Fragments arranged by size:
3041 2649 674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 4171 4553 5188
Size: 53 83 186 936 767 1871 382 635
Cuts at: 5188 275
Size: 1451
Fragments arranged by size:
1871 1451 936 767 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4797 5195 5469 5616 6027 4797
Size: 398 274 147 411 5134
Fragments arranged by size:
5134 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6139
Fragments arranged by size:
6139 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 1186
Size: 1177 5187
BseYI C'CCAG_C
Cuts at: 3028 3396 3541 5567 6063 3028
Size: 368 145 2026 496 3329
Fragments arranged by size:
3329 2026 496 368 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5791
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 3814 4575 5603 1540
Size: 275 186 55 1758 761 1028 2301
Fragments arranged by size:
2301 1758 1028 761 275 186 55
BsiHKAI G_wGCw'C
Cuts at: 4425 4510 5581 4425
Size: 85 1071 5208
Fragments arranged by size:
5208 1071 85
BsmI GAATG_Cn'
Cuts at: 3875 3968 3875
Size: 93 6271
BsmBI CGTCTCn'nnnn_
Cuts at: 2857 2971 3169 2857
Size: 114 198 6052
Fragments arranged by size:
6052 198 114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2494 2938 3035 3683 4425 4510 5581
Size: 399 704 444 97 648 742 85 1071
Cuts at: 5581 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 704 648 444 399 97
85
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 4253 5983 4253
Size: 1730 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 3719 844
Size: 2137 738 3489
Fragments arranged by size:
3489 2137 738
BsrBI CCG'CTC
Cuts at: 1394 1823 4251 1394
Size: 429 2428 3507
Fragments arranged by size:
3507 2428 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3304 3534 4859 5033 65
Size: 1183 2056 230 1325 174 1396
Fragments arranged by size:
2056 1396 1325 1183 230 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3594 96
Size: 3498 2866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 4424 5436 4424
Size: 1012 5352
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3249 794
Size: 2455 3909
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 3022 3541 3613 3022
Size: 519 72 5773
Fragments arranged by size:
5773 519 72
BstYI r'GATC_y
Cuts at: 1972 2542 2644 3001 3054 3520 3694 4061 4446
Size: 570 102 357 53 466 174 367 385
Cuts at: 4446 4463 5915 6001 6013 6359 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 570 466 385 367 357 346
174 102 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2273 2273
Size: 6364
BtgI C'CryG_G
Cuts at: 513 1065 2004 3570 513
Size: 552 939 1566 3307
Fragments arranged by size:
3307 1566 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3275 487
Size: 683 56 2049 3576
Fragments arranged by size:
3576 2049 683 56
BtsI GCAGTG_nn'
Cuts at: 2898 3880 4674 4694 2898
Size: 982 794 20 4568
Fragments arranged by size:
4568 982 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 2605 3668 2605
Size: 1063 5301
DraIII CAC_nnn'GTG
Cuts at: 2881 2881
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3391 5371 809
Size: 507 2075 1980 1802
Fragments arranged by size:
2075 1980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2069 2583
Size: 54 1425 50 275 18 223 16 514
Cuts at: 2583 2595 3012 3348 3435 3811 4700 6329 8
Size: 12 417 336 87 376 889 1629 43
Fragments arranged by size:
1629 1425 889 514 417 376 336 275
223 87 54 50 43 18 16 12
EagI C'GGCC_G
Cuts at: 1537 1812 2053 3811 1537
Size: 275 241 1758 4090
Fragments arranged by size:
4090 1758 275 241
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2432 2461 2727 4294 1353
Size: 906 173 29 266 1567 3423
Fragments arranged by size:
3423 1567 906 266 173 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3047 3628 4951 5325 5471 387
Size: 1064 138 1458 581 1323 374 146 1280
Fragments arranged by size:
1458 1323 1280 1064 581 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2421 2433 2442 2979 3237 4427
Size: 393 572 217 12 9 537 258 1190
Cuts at: 4427 5024 5811 6079 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1190 787 597 572 537 393 268
258 217 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2070 3377 3422 2070
Size: 1307 45 5012
Fragments arranged by size:
5012 1307 45
EcoO109I rG'GnC_Cy
Cuts at: 2934 3019 3032 2934
Size: 85 13 6266
Fragments arranged by size:
6266 85 13
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
EcoRV GAT'ATC
Cuts at: 2060 2060
Size: 6364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 3292 2143
Size: 1149 5215
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 3148 3614 5511 1678
Size: 1470 466 1897 2531
Fragments arranged by size:
2531 1897 1470 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2071 2103 2285 2317 5005
Size: 32 135 32 499 32 182 32 2688
Cuts at: 5005 5037 5079 5111 1373
Size: 32 42 32 2626
Fragments arranged by size:
2688 2626 499 182 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3556 3708 3954 669
Size: 988 281 1149 469 152 246 3079
Fragments arranged by size:
3079 1149 988 469 281 246 152
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 2979 3587 5478 5662 1305
Size: 1575 99 608 1891 184 2007
Fragments arranged by size:
2007 1891 1575 608 184 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2071 3350 3437 10
Size: 54 1425 343 239 1279 87 2937
Fragments arranged by size:
2937 1425 1279 343 239 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 4322 4681 4840 518
Size: 648 1611 1545 359 159 2042
Fragments arranged by size:
2042 1611 1545 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2690 2765 2957 3181 3249 3578 3780
Size: 576 508 75 192 224 68 329 202
Cuts at: 3780 4457 5605 5850 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 508 329 245 224
202 192 75 68
NcoI C'CATG_G
Cuts at: 513 1065 2004 3570 513
Size: 552 939 1566 3307
Fragments arranged by size:
3307 1566 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 3085 3722 5267 1132
Size: 97 1856 637 1545 2229
Fragments arranged by size:
2229 1856 1545 637 97
PasI CC'CwG_GG
Cuts at: 1348 3062 1348
Size: 1714 4650
PciI A'CATG_T
Cuts at: 3081 5263 3081
Size: 2182 4182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3262 1108
Size: 2154 4210
PfoI T'CCnGG_A
Cuts at: 2553 2925 3447 2553
Size: 372 522 5470
Fragments arranged by size:
5470 522 372
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3019 3019
Size: 6364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2934 3031 2934
Size: 97 6267
PstI C_TGCA'G
Cuts at: 830 2905 3245 3583 3716 830
Size: 2075 340 338 133 3478
Fragments arranged by size:
3478 2075 340 338 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2182 2690 2765 3181 3249 3578 2182
Size: 508 75 416 68 329 4968
Fragments arranged by size:
4968 508 416 329 75 68
RsrII CG'GwC_CG
Cuts at: 2588 2588
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SapI GCTCTTCn'nnn_
Cuts at: 2461 2461
Size: 6364
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SexAI A'CCwGG_T
Cuts at: 3021 3021
Size: 6364
SfcI C'TryA_G
Cuts at: 826 1045 2901 3241 3579 3712 4847 5528 5719
Size: 219 1856 340 338 133 1135 681 191
Cuts at: 5719 826
Size: 1471
Fragments arranged by size:
1856 1471 1135 681 340 338 219 191
133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4468 5369 5631 820
Size: 197 926 2525 901 262 1553
Fragments arranged by size:
2525 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2004 2066 2827 3006 3015 3283 3334
Size: 552 939 62 761 179 9 268 51
Cuts at: 3334 3570 3606 3803 513
Size: 236 36 197 3074
Fragments arranged by size:
3074 939 761 552 268 236 197 179
62 51 36 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 2812 3533 4391 4408 4561 4746
Size: 1255 1025 207 721 858 17 153 185
Cuts at: 4746 5191 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1025 858 721 445 207 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3594 4610 6322
Size: 274 80 33 51 1443 1617 1016 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
1712 1617 1443 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 6324 6356 3443
Size: 32 2849 32 3451
Fragments arranged by size:
3451 2849 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3104 1316
Size: 1788 4576
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3445 3445
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 2126 4493 2126
Size: 2367 3997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2301 4172 276
Size: 53 83 186 936 767 1871 2468
Fragments arranged by size:
2468 1871 936 767 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
SacI SacII SanDI SfiI SfoI SrfI SwaI