(Circular) (Six-base) MAPSORT of: pFN21AB3311.seq Check: 8198 from: 1 to: 6583 pFN21AB3311 6583 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3025 4393 278 Size: 53 83 186 936 1489 1368 2468 Fragments arranged by size: 2468 1489 1368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3012 3926 1937 Size: 1075 914 4594 Fragments arranged by size: 4594 1075 914 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3492 3500 3836 4646 6030 6298 1632 Size: 1860 8 336 810 1384 268 1917 Fragments arranged by size: 1917 1860 1384 810 336 268 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 2876 5482 6459 1203 Size: 1673 2606 977 1327 Fragments arranged by size: 2606 1673 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 3832 5312 3832 Size: 1480 5103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2988 1184 Size: 1804 4779 AlwNI CAG_nnn'CTG Cuts at: 3261 3296 3354 3583 3730 4326 5898 3261 Size: 35 58 229 147 596 1572 3946 Fragments arranged by size: 3946 1572 596 229 147 58 35 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3571 3892 4118 4270 6379 1493 Size: 2078 321 226 152 2109 1697 Fragments arranged by size: 2109 2078 1697 321 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2200 3908 1870 Size: 73 257 1708 4545 Fragments arranged by size: 4545 1708 257 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2275 2491 3758 3904 5359 Size: 325 217 627 488 216 1267 146 1455 Cuts at: 5359 618 Size: 1842 Fragments arranged by size: 1842 1455 1267 627 488 325 217 216 146 BanII G_rGCy'C Cuts at: 1391 2858 3146 3738 3902 1391 Size: 1467 288 592 164 4072 Fragments arranged by size: 4072 1467 592 288 164 BbeI G_GCGC'C Cuts at: 2279 2495 2279 Size: 216 6367 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3831 928 Size: 971 1932 3680 Fragments arranged by size: 3680 1932 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2469 2503 3501 3535 4774 Size: 34 50 34 448 34 998 34 1239 Cuts at: 4774 4808 1903 Size: 34 3678 Fragments arranged by size: 3678 1239 998 448 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3355 4475 5685 3355 Size: 1120 1210 4253 Fragments arranged by size: 4253 1210 1120 BclI T'GATC_A Cuts at: 1548 2253 3630 1548 Size: 705 1377 4501 Fragments arranged by size: 4501 1377 705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2622 2753 2765 Size: 107 122 71 631 605 950 131 12 Cuts at: 2765 2817 5194 136 Size: 52 2377 1525 Fragments arranged by size: 2377 1525 950 631 605 131 122 107 71 52 12 BglII A'GATC_T Cuts at: 3241 3780 6578 3241 Size: 539 2798 3246 Fragments arranged by size: 3246 2798 539 BlpI GC'TnA_GC Cuts at: 2666 3297 4000 2666 Size: 631 703 5249 Fragments arranged by size: 5249 703 631 Bme1580I G_kGCm'C Cuts at: 1790 2876 3763 4644 5800 1790 Size: 1086 887 881 1156 2573 Fragments arranged by size: 2573 1156 1086 887 881 BmgBI CAC'GTC Cuts at: 1318 2163 1318 Size: 845 5738 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3002 4314 5272 Size: 416 53 83 399 24 1585 1312 958 Cuts at: 5272 442 Size: 1753 Fragments arranged by size: 1753 1585 1312 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2025 2343 2350 3752 3782 5243 1239 Size: 786 318 7 1402 30 1461 2579 Fragments arranged by size: 2579 1461 1402 786 318 30 7 Bpu10I CC'TnA_GC Cuts at: 3604 3604 Size: 6583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 2738 4708 5573 5871 2201 Size: 537 1970 865 298 2913 Fragments arranged by size: 2913 1970 865 537 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5246 882 Size: 617 3747 2219 Fragments arranged by size: 3747 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2877 4374 493 Size: 711 1673 1497 2702 Fragments arranged by size: 2702 1673 1497 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2276 2492 3022 4390 Size: 53 83 186 936 743 216 530 1368 Cuts at: 4390 4772 5407 275 Size: 382 635 1451 Fragments arranged by size: 1451 1368 936 743 635 530 382 216 186 83 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3103 3106 3133 3136 1178 Size: 30 165 30 1700 3 27 3 4625 Fragments arranged by size: 4625 1700 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 3127 3174 1186 Size: 1093 848 47 4595 Fragments arranged by size: 4595 1093 848 47 BseYI C'CCAG_C Cuts at: 2171 2279 2390 2612 2677 2815 2828 2846 3354 Size: 108 111 222 65 138 13 18 508 Cuts at: 3354 5786 6282 2171 Size: 2432 496 2472 Fragments arranged by size: 2472 2432 508 496 222 138 111 108 65 18 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2984 3769 1163 Size: 573 1248 785 3977 Fragments arranged by size: 3977 1248 785 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2622 2655 4033 4794 5822 1540 Size: 275 186 621 33 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 621 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2858 4644 4729 5800 2858 Size: 1786 85 1071 3641 Fragments arranged by size: 3641 1786 1071 85 BsiWI C'GTAC_G Cuts at: 2730 2730 Size: 6583 BsmI GAATG_Cn' Cuts at: 3208 4094 4187 3208 Size: 886 93 5604 Fragments arranged by size: 5604 886 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3013 3013 Size: 6583 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2858 2876 3146 3738 3763 3902 4644 Size: 399 1068 18 270 592 25 139 742 Cuts at: 4644 4729 5800 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1068 742 592 399 270 139 85 25 18 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 2094 4472 6202 2094 Size: 2378 1730 2475 Fragments arranged by size: 2475 2378 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2936 3938 844 Size: 2092 1002 3489 Fragments arranged by size: 3489 2092 1002 BsrBI CCG'CTC Cuts at: 1394 1823 2572 4470 1394 Size: 429 749 1898 3507 Fragments arranged by size: 3507 1898 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3499 5078 5252 65 Size: 1183 2251 1579 174 1396 Fragments arranged by size: 2251 1579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2440 2555 2621 5227 5414 1700 Size: 110 630 115 66 2606 187 2869 Fragments arranged by size: 2869 2606 630 187 115 110 66 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 2559 2561 1925 Size: 634 2 5947 Fragments arranged by size: 5947 634 2 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6583 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2815 3754 2815 Size: 939 5644 BstYI r'GATC_y Cuts at: 1972 3241 3780 3913 4280 4665 4682 6134 6220 Size: 1269 539 133 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1269 539 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2085 2121 2547 2694 513 Size: 552 1020 36 426 147 4402 Fragments arranged by size: 4402 1020 552 426 147 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2488 487 Size: 683 56 1262 4582 Fragments arranged by size: 4582 1262 683 56 BtsI GCAGTG_nn' Cuts at: 2480 3465 4099 4893 4913 2480 Size: 985 634 794 20 4150 Fragments arranged by size: 4150 985 794 634 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3702 3887 3702 Size: 185 6398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 2619 2626 Size: 54 1425 50 275 18 723 66 7 Cuts at: 2626 2652 2745 4030 4919 6548 8 Size: 26 93 1285 889 1629 43 Fragments arranged by size: 1629 1425 1285 889 723 275 93 66 54 50 43 26 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2619 2652 4030 1537 Size: 275 807 33 1378 4090 Fragments arranged by size: 4090 1378 807 275 33 EarI CTCTTCn'nnn_ Cuts at: 1353 2579 3666 3866 4513 1353 Size: 1226 1087 200 647 3423 Fragments arranged by size: 3423 1226 1087 647 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2856 2856 Size: 6583 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 2343 2350 3492 3500 3752 3782 Size: 393 393 318 7 1142 8 252 30 Cuts at: 3782 3836 4646 5243 6030 6298 1239 Size: 54 810 597 787 268 1524 Fragments arranged by size: 1524 1142 810 787 597 393 393 318 268 252 54 30 8 7 EcoO109I rG'GnC_Cy Cuts at: 3751 3751 Size: 6583 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 EcoRV GAT'ATC Cuts at: 3595 3595 Size: 6583 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2279 2495 5730 1678 Size: 601 216 3235 2531 Fragments arranged by size: 3235 2531 601 216 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3103 3106 3135 3138 3190 Size: 32 135 32 1531 3 29 3 52 Cuts at: 3190 3222 3438 3470 3818 3850 5224 5256 5298 Size: 32 216 32 348 32 1374 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2626 1531 1374 348 216 135 52 42 32 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3927 4173 669 Size: 988 281 1989 246 3079 Fragments arranged by size: 3079 1989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KasI G'GCGC_C Cuts at: 2275 2491 2275 Size: 216 6367 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3049 5697 5881 1305 Size: 1744 2648 184 2007 Fragments arranged by size: 2648 2007 1744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 10 Size: 54 1425 343 915 3846 Fragments arranged by size: 3846 1425 915 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 4541 4900 5059 518 Size: 648 1233 2142 359 159 2042 Fragments arranged by size: 2142 2042 1233 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2245 2518 2657 2677 2696 2743 2764 3999 Size: 639 273 139 20 19 47 21 1235 Cuts at: 3999 4676 5824 6069 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 677 639 273 245 139 47 21 20 19 NaeI GCC'GGC Cuts at: 2442 2557 2623 2442 Size: 115 66 6402 Fragments arranged by size: 6402 115 66 NarI GG'CG_CC Cuts at: 2276 2492 2276 Size: 216 6367 NcoI C'CATG_G Cuts at: 513 1065 2121 513 Size: 552 1056 4975 Fragments arranged by size: 4975 1056 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NgoMIV G'CCGG_C Cuts at: 2440 2555 2621 2440 Size: 115 66 6402 Fragments arranged by size: 6402 115 66 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3305 3941 5486 1132 Size: 97 2076 636 1545 2229 Fragments arranged by size: 2229 2076 1545 636 97 PasI CC'CwG_GG Cuts at: 1348 3790 1348 Size: 2442 4141 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 3354 1108 Size: 2246 4337 PfoI T'CCnGG_A Cuts at: 3370 3864 3370 Size: 494 6089 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PmlI CAC'GTG Cuts at: 2877 2877 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3751 3751 Size: 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PspXI vC'TCGA_Gb Cuts at: 2200 2200 Size: 6583 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2090 2122 2090 Size: 32 6551 PstI C_TGCA'G Cuts at: 830 3286 3935 830 Size: 2456 649 3478 Fragments arranged by size: 3478 2456 649 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 SacI G_AGCT'C Cuts at: 2858 2858 Size: 6583 SacII CC_GC'GG Cuts at: 2697 2697 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SapI GCTCTTCn'nnn_ Cuts at: 2579 3666 3866 2579 Size: 1087 200 5296 Fragments arranged by size: 5296 1087 200 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 3041 4831 6543 3041 Size: 1790 1712 3081 Fragments arranged by size: 3081 1790 1712 SfcI C'TryA_G Cuts at: 826 1045 2151 3282 3931 5066 5747 5938 826 Size: 219 1106 1131 649 1135 681 191 1471 Fragments arranged by size: 1471 1135 1131 1106 681 649 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2765 2765 Size: 6583 SfoI GGC'GCC Cuts at: 2277 2493 2277 Size: 216 6367 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2200 2216 2717 4687 5588 5850 Size: 197 926 257 16 501 1970 901 262 Cuts at: 5850 820 Size: 1553 Fragments arranged by size: 1970 1553 926 901 501 262 257 197 16 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6583 StyI C'CwwG_G Cuts at: 513 1065 2121 2128 3747 4022 513 Size: 552 1056 7 1619 275 3074 Fragments arranged by size: 3074 1619 1056 552 275 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3250 3746 4610 4627 4780 4965 5410 Size: 1255 1670 496 864 17 153 185 445 Cuts at: 5410 325 Size: 1498 Fragments arranged by size: 1670 1498 1255 864 496 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 3039 4829 Size: 274 80 33 51 1443 426 636 1790 Cuts at: 4829 6541 96 Size: 1712 138 Fragments arranged by size: 1790 1712 1443 636 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6543 6575 6543 Size: 32 6551 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2119 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 2200 1943 Size: 257 6326 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 3366 4712 3366 Size: 1346 5237 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3023 4391 276 Size: 53 83 186 936 1489 1368 2468 Fragments arranged by size: 2468 1489 1368 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NruI NsiI PacI PspOMI PvuII RsrII SanDI SexAI SrfI SwaI