Restriction map for
pFN21AB3311
(Circular) (Six-base) MAPSORT of: pFN21AB3311.seq Check: 8198 from: 1 to: 6583
pFN21AB3311 6583 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3025 4393 278
Size: 53 83 186 936 1489 1368 2468
Fragments arranged by size:
2468 1489 1368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3012 3926 1937
Size: 1075 914 4594
Fragments arranged by size:
4594 1075 914
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3492 3500 3836 4646 6030 6298 1632
Size: 1860 8 336 810 1384 268 1917
Fragments arranged by size:
1917 1860 1384 810 336 268 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 2876 5482 6459 1203
Size: 1673 2606 977 1327
Fragments arranged by size:
2606 1673 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 3832 5312 3832
Size: 1480 5103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2988 1184
Size: 1804 4779
AlwNI CAG_nnn'CTG
Cuts at: 3261 3296 3354 3583 3730 4326 5898 3261
Size: 35 58 229 147 596 1572 3946
Fragments arranged by size:
3946 1572 596 229 147 58 35
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3571 3892 4118 4270 6379 1493
Size: 2078 321 226 152 2109 1697
Fragments arranged by size:
2109 2078 1697 321 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 3908 1870
Size: 73 257 1708 4545
Fragments arranged by size:
4545 1708 257 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2275 2491 3758 3904 5359
Size: 325 217 627 488 216 1267 146 1455
Cuts at: 5359 618
Size: 1842
Fragments arranged by size:
1842 1455 1267 627 488 325 217 216
146
BanII G_rGCy'C
Cuts at: 1391 2858 3146 3738 3902 1391
Size: 1467 288 592 164 4072
Fragments arranged by size:
4072 1467 592 288 164
BbeI G_GCGC'C
Cuts at: 2279 2495 2279
Size: 216 6367
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3831 928
Size: 971 1932 3680
Fragments arranged by size:
3680 1932 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2469 2503 3501 3535 4774
Size: 34 50 34 448 34 998 34 1239
Cuts at: 4774 4808 1903
Size: 34 3678
Fragments arranged by size:
3678 1239 998 448 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3355 4475 5685 3355
Size: 1120 1210 4253
Fragments arranged by size:
4253 1210 1120
BclI T'GATC_A
Cuts at: 1548 2253 3630 1548
Size: 705 1377 4501
Fragments arranged by size:
4501 1377 705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2622 2753 2765
Size: 107 122 71 631 605 950 131 12
Cuts at: 2765 2817 5194 136
Size: 52 2377 1525
Fragments arranged by size:
2377 1525 950 631 605 131 122 107
71 52 12
BglII A'GATC_T
Cuts at: 3241 3780 6578 3241
Size: 539 2798 3246
Fragments arranged by size:
3246 2798 539
BlpI GC'TnA_GC
Cuts at: 2666 3297 4000 2666
Size: 631 703 5249
Fragments arranged by size:
5249 703 631
Bme1580I G_kGCm'C
Cuts at: 1790 2876 3763 4644 5800 1790
Size: 1086 887 881 1156 2573
Fragments arranged by size:
2573 1156 1086 887 881
BmgBI CAC'GTC
Cuts at: 1318 2163 1318
Size: 845 5738
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3002 4314 5272
Size: 416 53 83 399 24 1585 1312 958
Cuts at: 5272 442
Size: 1753
Fragments arranged by size:
1753 1585 1312 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 2343 2350 3752 3782 5243 1239
Size: 786 318 7 1402 30 1461 2579
Fragments arranged by size:
2579 1461 1402 786 318 30 7
Bpu10I CC'TnA_GC
Cuts at: 3604 3604
Size: 6583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2738 4708 5573 5871 2201
Size: 537 1970 865 298 2913
Fragments arranged by size:
2913 1970 865 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5246 882
Size: 617 3747 2219
Fragments arranged by size:
3747 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2877 4374 493
Size: 711 1673 1497 2702
Fragments arranged by size:
2702 1673 1497 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2276 2492 3022 4390
Size: 53 83 186 936 743 216 530 1368
Cuts at: 4390 4772 5407 275
Size: 382 635 1451
Fragments arranged by size:
1451 1368 936 743 635 530 382 216
186 83 53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3103 3106 3133 3136 1178
Size: 30 165 30 1700 3 27 3 4625
Fragments arranged by size:
4625 1700 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 3127 3174 1186
Size: 1093 848 47 4595
Fragments arranged by size:
4595 1093 848 47
BseYI C'CCAG_C
Cuts at: 2171 2279 2390 2612 2677 2815 2828 2846 3354
Size: 108 111 222 65 138 13 18 508
Cuts at: 3354 5786 6282 2171
Size: 2432 496 2472
Fragments arranged by size:
2472 2432 508 496 222 138 111 108
65 18 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2984 3769 1163
Size: 573 1248 785 3977
Fragments arranged by size:
3977 1248 785 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2622 2655 4033 4794 5822 1540
Size: 275 186 621 33 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 621 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2858 4644 4729 5800 2858
Size: 1786 85 1071 3641
Fragments arranged by size:
3641 1786 1071 85
BsiWI C'GTAC_G
Cuts at: 2730 2730
Size: 6583
BsmI GAATG_Cn'
Cuts at: 3208 4094 4187 3208
Size: 886 93 5604
Fragments arranged by size:
5604 886 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3013 3013
Size: 6583
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 2876 3146 3738 3763 3902 4644
Size: 399 1068 18 270 592 25 139 742
Cuts at: 4644 4729 5800 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 592 399 270 139
85 25 18
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 2094 4472 6202 2094
Size: 2378 1730 2475
Fragments arranged by size:
2475 2378 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 3938 844
Size: 2092 1002 3489
Fragments arranged by size:
3489 2092 1002
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 4470 1394
Size: 429 749 1898 3507
Fragments arranged by size:
3507 1898 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3499 5078 5252 65
Size: 1183 2251 1579 174 1396
Fragments arranged by size:
2251 1579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2440 2555 2621 5227 5414 1700
Size: 110 630 115 66 2606 187 2869
Fragments arranged by size:
2869 2606 630 187 115 110 66
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 2559 2561 1925
Size: 634 2 5947
Fragments arranged by size:
5947 634 2
BssSI C'ACGA_G
Cuts at: 4643 5655 4643
Size: 1012 5571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6583
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 3754 2815
Size: 939 5644
BstYI r'GATC_y
Cuts at: 1972 3241 3780 3913 4280 4665 4682 6134 6220
Size: 1269 539 133 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1269 539 385 367 346 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2121 2547 2694 513
Size: 552 1020 36 426 147 4402
Fragments arranged by size:
4402 1020 552 426 147 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2488 487
Size: 683 56 1262 4582
Fragments arranged by size:
4582 1262 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3465 4099 4893 4913 2480
Size: 985 634 794 20 4150
Fragments arranged by size:
4150 985 794 634 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3702 3887 3702
Size: 185 6398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 2619 2626
Size: 54 1425 50 275 18 723 66 7
Cuts at: 2626 2652 2745 4030 4919 6548 8
Size: 26 93 1285 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 723 275 93 66
54 50 43 26 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2619 2652 4030 1537
Size: 275 807 33 1378 4090
Fragments arranged by size:
4090 1378 807 275 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 3666 3866 4513 1353
Size: 1226 1087 200 647 3423
Fragments arranged by size:
3423 1226 1087 647 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2856 2856
Size: 6583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2343 2350 3492 3500 3752 3782
Size: 393 393 318 7 1142 8 252 30
Cuts at: 3782 3836 4646 5243 6030 6298 1239
Size: 54 810 597 787 268 1524
Fragments arranged by size:
1524 1142 810 787 597 393 393 318
268 252 54 30 8 7
EcoO109I rG'GnC_Cy
Cuts at: 3751 3751
Size: 6583
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
EcoRV GAT'ATC
Cuts at: 3595 3595
Size: 6583
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 2279 2495 5730 1678
Size: 601 216 3235 2531
Fragments arranged by size:
3235 2531 601 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3103 3106 3135 3138 3190
Size: 32 135 32 1531 3 29 3 52
Cuts at: 3190 3222 3438 3470 3818 3850 5224 5256 5298
Size: 32 216 32 348 32 1374 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2626 1531 1374 348 216 135 52 42
32 32 32 32 32 32 32 29
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3927 4173 669
Size: 988 281 1989 246 3079
Fragments arranged by size:
3079 1989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KasI G'GCGC_C
Cuts at: 2275 2491 2275
Size: 216 6367
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3049 5697 5881 1305
Size: 1744 2648 184 2007
Fragments arranged by size:
2648 2007 1744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3846
Fragments arranged by size:
3846 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 4541 4900 5059 518
Size: 648 1233 2142 359 159 2042
Fragments arranged by size:
2142 2042 1233 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2518 2657 2677 2696 2743 2764 3999
Size: 639 273 139 20 19 47 21 1235
Cuts at: 3999 4676 5824 6069 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 639 273 245 139
47 21 20 19
NaeI GCC'GGC
Cuts at: 2442 2557 2623 2442
Size: 115 66 6402
Fragments arranged by size:
6402 115 66
NarI GG'CG_CC
Cuts at: 2276 2492 2276
Size: 216 6367
NcoI C'CATG_G
Cuts at: 513 1065 2121 513
Size: 552 1056 4975
Fragments arranged by size:
4975 1056 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NgoMIV G'CCGG_C
Cuts at: 2440 2555 2621 2440
Size: 115 66 6402
Fragments arranged by size:
6402 115 66
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 3305 3941 5486 1132
Size: 97 2076 636 1545 2229
Fragments arranged by size:
2229 2076 1545 636 97
PasI CC'CwG_GG
Cuts at: 1348 3790 1348
Size: 2442 4141
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3354 1108
Size: 2246 4337
PfoI T'CCnGG_A
Cuts at: 3370 3864 3370
Size: 494 6089
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PmlI CAC'GTG
Cuts at: 2877 2877
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3751 3751
Size: 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PspXI vC'TCGA_Gb
Cuts at: 2200 2200
Size: 6583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2090 2122 2090
Size: 32 6551
PstI C_TGCA'G
Cuts at: 830 3286 3935 830
Size: 2456 649 3478
Fragments arranged by size:
3478 2456 649
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
SacI G_AGCT'C
Cuts at: 2858 2858
Size: 6583
SacII CC_GC'GG
Cuts at: 2697 2697
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SapI GCTCTTCn'nnn_
Cuts at: 2579 3666 3866 2579
Size: 1087 200 5296
Fragments arranged by size:
5296 1087 200
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 3041 4831 6543 3041
Size: 1790 1712 3081
Fragments arranged by size:
3081 1790 1712
SfcI C'TryA_G
Cuts at: 826 1045 2151 3282 3931 5066 5747 5938 826
Size: 219 1106 1131 649 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 1106 681 649 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2765 2765
Size: 6583
SfoI GGC'GCC
Cuts at: 2277 2493 2277
Size: 216 6367
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 2216 2717 4687 5588 5850
Size: 197 926 257 16 501 1970 901 262
Cuts at: 5850 820
Size: 1553
Fragments arranged by size:
1970 1553 926 901 501 262 257 197
16
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6583
StyI C'CwwG_G
Cuts at: 513 1065 2121 2128 3747 4022 513
Size: 552 1056 7 1619 275 3074
Fragments arranged by size:
3074 1619 1056 552 275 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3250 3746 4610 4627 4780 4965 5410
Size: 1255 1670 496 864 17 153 185 445
Cuts at: 5410 325
Size: 1498
Fragments arranged by size:
1670 1498 1255 864 496 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 3039 4829
Size: 274 80 33 51 1443 426 636 1790
Cuts at: 4829 6541 96
Size: 1712 138
Fragments arranged by size:
1790 1712 1443 636 426 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6543 6575 6543
Size: 32 6551
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2119
Size: 6583
XhoI C'TCGA_G
Cuts at: 1943 2200 1943
Size: 257 6326
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 3366 4712 3366
Size: 1346 5237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3023 4391 276
Size: 53 83 186 936 1489 1368 2468
Fragments arranged by size:
2468 1489 1368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI
NruI NsiI PacI PspOMI PvuII RsrII SanDI SexAI
SrfI SwaI