Restriction map for
pFN21AB3307
(Circular) (Six-base) MAPSORT of: pFN21AB3307.seq Check: 2094 from: 1 to: 6859
pFN21AB3307 6859 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2081 4202 1937
Size: 144 2121 4594
Fragments arranged by size:
4594 2121 144
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 2787 3581 4922 6306 6574 1632
Size: 1154 1 794 1341 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1154 794 268 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 3126 5758 6735 1203
Size: 1923 2632 977 1327
Fragments arranged by size:
2632 1923 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 3661 1166
Size: 2495 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3747 3826 1184
Size: 2563 79 4217
Fragments arranged by size:
4217 2563 79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 6827
AlwNI CAG_nnn'CTG
Cuts at: 2285 2294 2348 2432 2576 3152 3270 3559 3910
Size: 9 54 84 144 576 118 289 351
Cuts at: 3910 3950 4111 4602 6174 2285
Size: 40 161 491 1572 2970
Fragments arranged by size:
2970 1572 576 491 351 289 161 144
118 84 54 40 9
ApaI G_GGCC'C
Cuts at: 2732 2812 3985 2732
Size: 80 1173 5606
Fragments arranged by size:
5606 1173 80
ApaLI G'TGCA_C
Cuts at: 3629 4916 6072 3629
Size: 1287 1156 4416
Fragments arranged by size:
4416 1287 1156
ApoI r'AATT_y
Cuts at: 1493 3566 4152 4168 4394 4546 6655 1493
Size: 2073 586 16 226 152 2109 1697
Fragments arranged by size:
2109 2073 1697 586 226 152 16
AseI AT'TA_AT
Cuts at: 160 1032 5413 160
Size: 872 4381 1606
Fragments arranged by size:
4381 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 4059 4184 1870
Size: 73 2116 125 4545
Fragments arranged by size:
4545 2116 125 73
AvrII C'CTAG_G
Cuts at: 2443 2443
Size: 6859
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2274 2307 2274
Size: 33 6826
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3602 3731 4180 5635 618
Size: 325 217 627 1815 129 449 1455 1842
Fragments arranged by size:
1842 1815 1455 627 449 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2531 2732 2812 2937 2960 3310 3985 4178
Size: 1140 201 80 125 23 350 675 193
Cuts at: 4178 1391
Size: 4072
Fragments arranged by size:
4072 1140 675 350 201 193 125 80
23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2793 3129 928
Size: 971 894 336 4658
Fragments arranged by size:
4658 971 894 336
BbvCI CC'TCA_GC
Cuts at: 2864 3962 2864
Size: 1098 5761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2061 2095 3342 3376 5050 5084 1903
Size: 34 124 34 1247 34 1674 34 3678
Fragments arranged by size:
3678 1674 1247 124 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4751 5961 4751
Size: 1210 5649
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3832 3896 5470
Size: 107 122 71 631 605 2160 64 1574
Cuts at: 5470 136
Size: 1525
Fragments arranged by size:
2160 1574 1525 631 605 122 107 71
64
BglII A'GATC_T
Cuts at: 6854 6854
Size: 6859
BlpI GC'TnA_GC
Cuts at: 4276 4276
Size: 6859
Bme1580I G_kGCm'C
Cuts at: 1790 2732 2812 3105 3633 3736 3985 4920 6076
Size: 942 80 293 528 103 249 935 1156
Cuts at: 6076 1790
Size: 2573
Fragments arranged by size:
2573 1156 942 935 528 293 249 103
80
BmgBI CAC'GTC
Cuts at: 1318 3628 1318
Size: 2310 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3115 4590 5548
Size: 416 53 83 399 24 1698 1475 958
Cuts at: 5548 442
Size: 1753
Fragments arranged by size:
1753 1698 1475 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3785
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2223 2345 2389 3615 3695 3758 5519 1239
Size: 984 122 44 1226 80 63 1761 2579
Fragments arranged by size:
2579 1761 1226 984 122 80 63 44
Bpu10I CC'TnA_GC
Cuts at: 2139 2864 3962 2139
Size: 725 1098 5036
Fragments arranged by size:
5036 1098 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2283 3200 4984 5849 6147 2283
Size: 917 1784 865 298 2995
Fragments arranged by size:
2995 1784 917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5522 882
Size: 617 4023 2219
Fragments arranged by size:
4023 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 6853 6853
Size: 6859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4666 5048 5683 275
Size: 53 83 186 936 3133 382 635 1451
Fragments arranged by size:
3133 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2589 5292 5690 5964 6111 6522 2589
Size: 2703 398 274 147 411 2926
Fragments arranged by size:
2926 2703 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6634
Fragments arranged by size:
6634 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2678 1186
Size: 952 540 5367
Fragments arranged by size:
5367 952 540
BseYI C'CCAG_C
Cuts at: 3164 3984 6062 6558 3164
Size: 820 2078 496 3465
Fragments arranged by size:
3465 2078 820 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3158 3219 4124 1163
Size: 573 1422 61 905 3898
Fragments arranged by size:
3898 1422 905 573 61
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4309 5070 6098 1540
Size: 275 186 2308 761 1028 2301
Fragments arranged by size:
2308 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 3633 3771 4920 5005 6076 2937
Size: 696 138 1149 85 1071 3720
Fragments arranged by size:
3720 1149 1071 696 138 85
BsmI GAATG_Cn'
Cuts at: 4370 4463 4370
Size: 93 6766
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2531 2732 2812 2937 2960 3105 3310
Size: 399 741 201 80 125 23 145 205
Cuts at: 3310 3633 3736 3771 3985 4178 4920 5005 6076
Size: 323 103 35 214 193 742 85 1071
Cuts at: 6076 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 741 399 323 214 205
201 193 145 125 103 85 80 35
23
BspEI T'CCGG_A
Cuts at: 2589 6522 2589
Size: 3933 2926
BspHI T'CATG_A
Cuts at: 4748 6478 4748
Size: 1730 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4214 844
Size: 3370 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 2329 2551 4746 1394
Size: 429 284 222 222 2195 3507
Fragments arranged by size:
3507 2195 429 284 222 222
BsrDI GCAATG_nn'
Cuts at: 65 1248 5354 5528 65
Size: 1183 4106 174 1396
Fragments arranged by size:
4106 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2910 3258 3651 5503 5690 1700
Size: 110 1100 348 393 1852 187 2869
Fragments arranged by size:
2869 1852 1100 393 348 187 110
BsrGI T'GTAC_A
Cuts at: 96 3392 96
Size: 3296 3563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 2982 4129 4919 5931 2982
Size: 1147 790 1012 3910
Fragments arranged by size:
3910 1147 1012 790
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2285 2294 3910 794
Size: 1491 9 1616 3743
Fragments arranged by size:
3743 1616 1491 9
BstBI TT'CG_AA
Cuts at: 5663 5663
Size: 6859
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6859
BstYI r'GATC_y
Cuts at: 1972 3266 3417 4189 4556 4941 4958 6410 6496
Size: 1294 151 772 367 385 17 1452 86
Cuts at: 6496 6508 6854 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1294 772 385 367 346 151
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4095 4095
Size: 6859
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5368
Fragments arranged by size:
5368 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 2397 2819 3238 3771 4375 5169 5189 2295
Size: 102 422 419 533 604 794 20 3965
Fragments arranged by size:
3965 794 604 533 422 419 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2079 2114 529
Size: 35 1515 35 5274
Fragments arranged by size:
5274 1515 35 35
DraI TTT'AAA
Cuts at: 4163 4163
Size: 6859
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3494 3653 3824
Size: 54 1425 50 275 18 1664 159 171
Cuts at: 3824 4018 4306 5195 6824 8
Size: 194 288 889 1629 43
Fragments arranged by size:
1664 1629 1425 889 288 275 194 171
159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4306 1537
Size: 275 2494 4090
Fragments arranged by size:
4090 2494 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 4789 1353
Size: 1589 1847 3423
Fragments arranged by size:
3423 1847 1589
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3464 3965 5446 5820 5966 387
Size: 1064 138 1875 501 1481 374 146 1280
Fragments arranged by size:
1875 1481 1280 1064 501 374 146 138
EcoICRI GAG'CTC
Cuts at: 2935 2935
Size: 6859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2345 2389 2786 2787 3581 3615
Size: 393 591 122 44 397 1 794 34
Cuts at: 3615 3695 3758 4922 5519 6306 6574 1239
Size: 80 63 1164 597 787 268 1524
Fragments arranged by size:
1524 1164 794 787 597 591 397 393
268 122 80 63 44 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2507 2590 2723 2794 3551 2120
Size: 387 83 133 71 757 5428
Fragments arranged by size:
5428 757 387 133 83 71
EcoO109I rG'GnC_Cy
Cuts at: 2728 2729 2808 2809 3514 3544 3556 3583 4135
Size: 1 79 1 705 30 12 27 552
Cuts at: 4135 2728
Size: 5452
Fragments arranged by size:
5452 705 552 79 30 27 12 1
1
EcoRI G'AATT_C
Cuts at: 4168 4546 4168
Size: 378 6481
EcoRV GAT'ATC
Cuts at: 2193 2193
Size: 6859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 6859
FseI GG_CCGG'CC
Cuts at: 3655 3655
Size: 6859
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 6006 1678
Size: 4328 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3575 3607 3693 3725 3932
Size: 32 135 32 2003 32 86 32 207
Cuts at: 3932 3945 3964 3977 5500 5532 5574 5606 1373
Size: 13 19 13 1523 32 42 32 2626
Fragments arranged by size:
2626 2003 1523 207 135 86 42 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2082 2716 4203 4449 669
Size: 988 281 144 634 1487 246 3079
Fragments arranged by size:
3079 1487 988 634 281 246 144
HindIII A'AGCT_T
Cuts at: 748 2648 748
Size: 1900 4959
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 4458 4458
Size: 6859
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2705 3712 5973 6157 1305
Size: 1400 1007 2261 184 2007
Fragments arranged by size:
2261 2007 1400 1007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3826 4020 10
Size: 54 1425 343 1994 194 2849
Fragments arranged by size:
2849 1994 1425 343 194 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2791 3661 3663 3863 4817 5176 5335
Size: 648 1625 870 2 200 954 359 159
Cuts at: 5335 518
Size: 2042
Fragments arranged by size:
2042 1625 954 870 648 359 200 159
2
MspA1I CmG'CkG
Cuts at: 1606 2432 2573 3406 4275 4952 6100 6345 1606
Size: 826 141 833 869 677 1148 245 2120
Fragments arranged by size:
2120 1148 869 833 826 677 245 141
NaeI GCC'GGC
Cuts at: 3653 3653
Size: 6859
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5368
Fragments arranged by size:
5368 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6859
NgoMIV G'CCGG_C
Cuts at: 3651 3651
Size: 6859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 3130 4217 5762 1132
Size: 97 1901 1087 1545 2229
Fragments arranged by size:
2229 1901 1545 1087 97
PasI CC'CwG_GG
Cuts at: 1348 2960 3579 3646 1348
Size: 1612 619 67 4561
Fragments arranged by size:
4561 1612 619 67
PciI A'CATG_T
Cuts at: 3126 5758 3126
Size: 2632 4227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 3270 3853 1108
Size: 1311 851 583 4114
Fragments arranged by size:
4114 1311 851 583
PfoI T'CCnGG_A
Cuts at: 2200 2200
Size: 6859
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 2951 4928 4960 6441 6473 2919
Size: 32 1977 32 1481 32 3305
Fragments arranged by size:
3305 1977 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3514 3544 3583 4135 3514
Size: 30 39 552 6238
Fragments arranged by size:
6238 552 39 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PspOMI G'GGCC_C
Cuts at: 2728 2808 3981 2728
Size: 80 1173 5606
Fragments arranged by size:
5606 1173 80
PstI C_TGCA'G
Cuts at: 830 2290 2462 2826 3922 4211 830
Size: 1460 172 364 1096 289 3478
Fragments arranged by size:
3478 1460 1096 364 289 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2432 2573 3406 2432
Size: 141 833 5885
Fragments arranged by size:
5885 833 141
SacI G_AGCT'C
Cuts at: 2937 2937
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 2080 4201 1936
Size: 144 2121 4594
Fragments arranged by size:
4594 2121 144
SanDI GG'GwC_CC
Cuts at: 3514 4135 3514
Size: 621 6238
SapI GCTCTTCn'nnn_
Cuts at: 2942 2942
Size: 6859
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 5107 6819 5107
Size: 1712 5147
SfcI C'TryA_G
Cuts at: 826 1045 2286 2458 2822 3918 4121 4207 5342
Size: 219 1241 172 364 1096 203 86 1135
Cuts at: 5342 6023 6214 826
Size: 681 191 1471
Fragments arranged by size:
1471 1241 1135 1096 681 364 219 203
191 172 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 2910 1700
Size: 1210 5649
SmaI CCC'GGG
Cuts at: 1872 4186 1872
Size: 2314 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 3215 4059 4963 5864 6126
Size: 197 926 355 917 844 904 901 262
Cuts at: 6126 820
Size: 1553
Fragments arranged by size:
1553 926 917 904 901 844 355 262
197
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 3007 152
Size: 2855 4004
SphI G_CATG'C
Cuts at: 1132 4217 1132
Size: 3085 3774
SspI AAT'ATT
Cuts at: 5 52 4783 5
Size: 47 4731 2081
Fragments arranged by size:
4731 2081 47
StuI AGG'CCT
Cuts at: 1517 3160 1517
Size: 1643 5216
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2334 2443 4075 4298 513
Size: 552 939 37 293 109 1632 223 3074
Fragments arranged by size:
3074 1632 939 552 293 223 109 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 2688 4886 4903 5056 5241 5686
Size: 1255 846 262 2198 17 153 185 445
Cuts at: 5686 325
Size: 1498
Fragments arranged by size:
2198 1498 1255 846 445 262 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2471 3392 5105
Size: 274 80 33 51 1443 494 921 1713
Cuts at: 5105 6817 96
Size: 1712 138
Fragments arranged by size:
1713 1712 1443 921 494 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6819 6851 6819
Size: 32 6827
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6859
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 2915
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 4059 1943
Size: 2116 4743
XmaI C'CCGG_G
Cuts at: 1870 4184 1870
Size: 2314 4545
XmnI GAAnn'nnTTC
Cuts at: 4988 4988
Size: 6859
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI