Restriction map for
pFN21AB3304
(Circular) (Six-base) MAPSORT of: pFN21AB3304.seq Check: 9579 from: 1 to: 5848
pFN21AB3304 5848 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2316 3191 1937
Size: 379 875 4594
Fragments arranged by size:
4594 875 379
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3911 5295 5563 1632
Size: 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 268
AfeI AGC'GCT
Cuts at: 2091 2308 2091
Size: 217 5631
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 3073 4747 5724 1203
Size: 1870 1674 977 1327
Fragments arranged by size:
1870 1674 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 2206 2645 2906 3591 5163 2206
Size: 439 261 685 1572 2891
Fragments arranged by size:
2891 1572 685 439 261
ApaLI G'TGCA_C
Cuts at: 2119 3905 5061 2119
Size: 1786 1156 2906
Fragments arranged by size:
2906 1786 1156
ApoI r'AATT_y
Cuts at: 1493 2747 3157 3383 3535 5644 1493
Size: 1254 410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 410 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 3167 1391
Size: 673 1103 4072
Fragments arranged by size:
4072 1103 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BbvCI CC'TCA_GC
Cuts at: 2310 2310
Size: 5848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 2536 1548
Size: 988 4860
BfrBI ATG'CAT
Cuts at: 2813 2813
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2123 3909 5065 1790
Size: 333 1786 1156 2573
Fragments arranged by size:
2573 1786 1156 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2264 3579 4537
Size: 416 53 83 399 24 847 1315 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1315 958 847 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4508 1239
Size: 3269 2579
Bpu10I CC'TnA_GC
Cuts at: 2310 2310
Size: 5848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 3973 4838 5136 2218
Size: 1755 865 298 2930
Fragments arranged by size:
2930 1755 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2131 2476 4511 882
Size: 617 632 345 2035 2219
Fragments arranged by size:
2219 2035 632 617 345
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 1178
Size: 30 165 30 1075 30 4518
Fragments arranged by size:
4518 1075 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2198 2244 2247 1186
Size: 862 150 46 3 4787
Fragments arranged by size:
4787 862 150 46 3
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2846 2876 1163
Size: 573 396 714 30 4135
Fragments arranged by size:
4135 714 573 396 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2686 3298 4059 5087 1540
Size: 275 186 685 612 761 1028 2301
Fragments arranged by size:
2301 1028 761 685 612 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2123 3909 3994 5065 2064
Size: 59 1786 85 1071 2847
Fragments arranged by size:
2847 1786 1071 85 59
BsmI GAATG_Cn'
Cuts at: 2147 3359 3452 2147
Size: 1212 93 4543
Fragments arranged by size:
4543 1212 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2540
Size: 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2123 3167 3909 3994 5065 1391
Size: 399 274 59 1044 742 85 1071 2174
Fragments arranged by size:
2174 1071 1044 742 399 274 85 59
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3006 3027 3908 4920 3006
Size: 21 881 1012 3934
Fragments arranged by size:
3934 1012 881 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2272
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843
Size: 1206 367 385 17 1452 86 12 346
Cuts at: 5843 1972
Size: 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2153 2153
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 4219
Fragments arranged by size:
4219 939 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 3364 4158 4178 2121
Size: 1243 794 20 3791
Fragments arranged by size:
3791 1243 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3004 3039 529
Size: 35 2440 35 3338
Fragments arranged by size:
3338 2440 35 35
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 3295 4184
Size: 54 1425 50 275 18 549 916 889
Cuts at: 4184 5813 8
Size: 1629 43
Fragments arranged by size:
1629 1425 916 889 549 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 3778 1353
Size: 1575 850 3423
Fragments arranged by size:
3423 1575 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 5848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3911 4508 5295 5563 1239
Size: 393 2279 597 787 268 1524
Fragments arranged by size:
2279 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2960 2960
Size: 5848
EcoRI G'AATT_C
Cuts at: 2747 3157 3535 2747
Size: 410 378 5060
Fragments arranged by size:
5060 410 378
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2093 2310 4995 1678
Size: 415 217 2685 2531
Fragments arranged by size:
2685 2531 415 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 2239 2271 2286
Size: 32 135 32 616 32 19 32 15
Cuts at: 2286 2318 2321 2353 2986 3018 4489 4521 4563
Size: 32 3 32 633 32 1471 32 42
Cuts at: 4563 4595 1373
Size: 32 2626
Fragments arranged by size:
2626 1471 633 616 135 42 32 32
32 32 32 32 32 32 32 19
15 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 3192 3438 669
Size: 988 281 625 629 246 3079
Fragments arranged by size:
3079 988 629 625 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 2563 3438 2563
Size: 875 4973
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 4962 5146 1305
Size: 849 2808 184 2007
Fragments arranged by size:
2808 2007 849 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 3806 4165 4324 518
Size: 648 1261 1379 359 159 2042
Fragments arranged by size:
2042 1379 1261 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2903 3264 3941 5089 5334 1606
Size: 1297 361 677 1148 245 2120
Fragments arranged by size:
2120 1297 1148 677 361 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 4219
Fragments arranged by size:
4219 939 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NsiI A_TGCA'T
Cuts at: 2815 2815
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 3206 4751 1132
Size: 97 1977 1545 2229
Fragments arranged by size:
2229 1977 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2552 1613
Size: 939 4909
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 2211 3200 830
Size: 1381 989 3478
Fragments arranged by size:
3478 1381 989
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2903 2903
Size: 5848
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2207 2695 3196 4331 5012 5203 826
Size: 219 1162 488 501 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 681 501 488 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 3952 4853 5115 820
Size: 197 926 290 1719 901 262 1553
Fragments arranged by size:
1719 1553 926 901 290 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 3287 513
Size: 552 939 138 1145 3074
Fragments arranged by size:
3074 1145 939 552 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 2458 2700 2910 3072 3875 3892
Size: 1255 698 180 242 210 162 803 17
Cuts at: 3892 4045 4230 4675 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 803 698 445 242 210 185
180 162 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3120 4094 5806
Size: 274 80 33 51 1443 1143 974 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1443 1143 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2478 2510 5808 5840 2478
Size: 32 3298 32 2486
Fragments arranged by size:
3298 2486 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3075 3977 3075
Size: 902 4946
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI