Restriction map for
pFN21AB3300
(Circular) (Six-base) MAPSORT of: pFN21AB3300.seq Check: 2608 from: 1 to: 6874
pFN21AB3300 6874 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4127 4127
Size: 6874
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 4217 1937
Size: 625 1655 4594
Fragments arranged by size:
4594 1655 625
Acc65I G'GTAC_C
Cuts at: 4195 4195
Size: 6874
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 2843 2876 3008 4937 6321 6589 1632
Size: 809 402 33 132 1929 1384 268 1917
Fragments arranged by size:
1929 1917 1384 809 402 268 132 33
AfeI AGC'GCT
Cuts at: 3527 3527
Size: 6874
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 5773 6750 1203
Size: 4570 977 1327
Fragments arranged by size:
4570 1327 977
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 3496 5603 3496
Size: 2107 4767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2183 2331 3384 1184
Size: 999 148 1053 4674
Fragments arranged by size:
4674 1053 999 148
AlwNI CAG_nnn'CTG
Cuts at: 2509 2932 3028 3181 3298 3343 3556 3851 3889
Size: 423 96 153 117 45 213 295 38
Cuts at: 3889 4111 4617 6189 2509
Size: 222 506 1572 3194
Fragments arranged by size:
3194 1572 506 423 295 222 213 153
117 96 45 38
ApaI G_GGCC'C
Cuts at: 2718 3535 2718
Size: 817 6057
ApaLI G'TGCA_C
Cuts at: 4931 6087 4931
Size: 1156 5718
ApoI r'AATT_y
Cuts at: 1493 4183 4409 4561 6670 1493
Size: 2690 226 152 2109 1697
Fragments arranged by size:
2690 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 2807 4199 1870
Size: 73 754 110 1392 4545
Fragments arranged by size:
4545 1392 754 110 73
BamHI G'GATC_C
Cuts at: 3018 3906 4204 4571 3018
Size: 888 298 367 5321
Fragments arranged by size:
5321 888 367 298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2585 2820 4195 5650 618
Size: 325 217 627 798 235 1375 1455 1842
Fragments arranged by size:
1842 1455 1375 798 627 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2127 2718 3535 3591 3818 4193 1391
Size: 736 591 817 56 227 375 4072
Fragments arranged by size:
4072 817 736 591 375 227 56
BbeI G_GCGC'C
Cuts at: 2824 2824
Size: 6874
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5903
BbvCI CC'TCA_GC
Cuts at: 3536 3536
Size: 6874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5065 5099 1903
Size: 34 50 34 3044 34 3678
Fragments arranged by size:
3678 3044 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4766 5976 4766
Size: 1210 5664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6874
BfrBI ATG'CAT
Cuts at: 3406 3406
Size: 6874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5485 136
Size: 107 122 71 631 605 3813 1525
Fragments arranged by size:
3813 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2263 6869 2263
Size: 4606 2268
BlpI GC'TnA_GC
Cuts at: 2537 3753 3803 4291 2537
Size: 1216 50 488 5120
Fragments arranged by size:
5120 1216 488 50
Bme1580I G_kGCm'C
Cuts at: 1790 2718 3535 4935 6091 1790
Size: 928 817 1400 1156 2573
Fragments arranged by size:
2573 1400 1156 928 817
BmgBI CAC'GTC
Cuts at: 1318 3152 4081 1318
Size: 1834 929 4111
Fragments arranged by size:
4111 1834 929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 2840 4605
Size: 416 53 83 399 24 611 812 1765
Cuts at: 4605 5563 442
Size: 958 1753
Fragments arranged by size:
1765 1753 958 812 611 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 6874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2306 2375 2549 2693 2819 2942 3368
Size: 839 228 69 174 144 126 123 426
Cuts at: 3368 3488 3533 3758 4028 5534 1239
Size: 120 45 225 270 1506 2579
Fragments arranged by size:
2579 1506 839 426 270 228 225 174
144 126 123 120 69 45
Bpu10I CC'TnA_GC
Cuts at: 3536 3536
Size: 6874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2716 4999 5864 6162 2716
Size: 2283 865 298 3428
Fragments arranged by size:
3428 2283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5537 882
Size: 617 4038 2219
Fragments arranged by size:
4038 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4665 493
Size: 711 3461 2702
Fragments arranged by size:
3461 2702 711
BsaBI GATnn'nnATC
Cuts at: 6868 6868
Size: 6874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2547 2821 3840 4681
Size: 53 83 186 936 1014 274 1019 841
Cuts at: 4681 5063 5698 275
Size: 382 635 1451
Fragments arranged by size:
1451 1019 1014 936 841 635 382 274
186 83 53
BsaWI w'CCGG_w
Cuts at: 2019 3873 5307 5705 5979 6126 6537 2019
Size: 1854 1434 398 274 147 411 2356
Fragments arranged by size:
2356 1854 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 2897 2927 3146
Size: 30 165 30 1329 30 135 30 219
Cuts at: 3146 3176 3731 3761 1178
Size: 30 555 30 4291
Fragments arranged by size:
4291 1329 555 219 165 135 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2753 2921 3143 3755 1186
Size: 868 699 168 222 612 4305
Fragments arranged by size:
4305 868 699 612 222 168
BseYI C'CCAG_C
Cuts at: 2087 2385 2850 3528 4146 6077 6573 2087
Size: 298 465 678 618 1931 496 2388
Fragments arranged by size:
2388 1931 678 618 496 465 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4034 1163
Size: 573 2298 4003
Fragments arranged by size:
4003 2298 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4324 5085 6113 1540
Size: 275 186 2323 761 1028 2301
Fragments arranged by size:
2323 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2847 2943 3591 3759 4935 5020 6091 2127
Size: 720 96 648 168 1176 85 1071 2910
Fragments arranged by size:
2910 1176 1071 720 648 168 96 85
BsmI GAATG_Cn'
Cuts at: 4385 4478 4385
Size: 93 6781
BsmBI CGTCTCn'nnnn_
Cuts at: 3847 3847
Size: 6874
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2718 2847 2943 3535 3591 3759
Size: 399 337 591 129 96 592 56 168
Cuts at: 3759 3818 4193 4935 5020 6091 1391
Size: 59 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 591 399 375 337
168 129 96 85 59 56
BspEI T'CCGG_A
Cuts at: 2019 3873 6537 2019
Size: 1854 2664 2356
Fragments arranged by size:
2664 2356 1854
BspHI T'CATG_A
Cuts at: 4763 6493 4763
Size: 1730 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3771 3807 4127 4229 844
Size: 2927 36 320 102 3489
Fragments arranged by size:
3489 2927 320 102 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2965 3992 4761 1394
Size: 429 188 954 1027 769 3507
Fragments arranged by size:
3507 1027 954 769 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 5369 5543 65
Size: 1183 4121 174 1396
Fragments arranged by size:
4121 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2588 3201 5518 5705 1700
Size: 110 778 613 2317 187 2869
Fragments arranged by size:
2869 2317 778 613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6874
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 3863 4934 5946 3863
Size: 1071 1012 4791
Fragments arranged by size:
4791 1071 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3178 3763 794
Size: 2384 585 3905
Fragments arranged by size:
3905 2384 585
BstBI TT'CG_AA
Cuts at: 2452 2521 5678 2452
Size: 69 3157 3648
Fragments arranged by size:
3648 3157 69
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstYI r'GATC_y
Cuts at: 1972 2263 2487 2886 3018 3339 3867 3906 4204
Size: 291 224 399 132 321 528 39 298
Cuts at: 4204 4571 4956 4973 6425 6511 6523 6869 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 528 399 385 367 346 321
298 291 224 132 86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2439 2556 2570 2718 513
Size: 552 1374 117 14 148 4669
Fragments arranged by size:
4669 1374 552 148 117 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3371 4126 487
Size: 683 56 2145 755 3235
Fragments arranged by size:
3235 2145 755 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 4103 4109 4390 5184 5204 2592
Size: 1511 6 281 794 20 4262
Fragments arranged by size:
4262 1511 794 281 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2458 2493 529
Size: 35 1894 35 4910
Fragments arranged by size:
4910 1894 35 35
DraI TTT'AAA
Cuts at: 4178 4178
Size: 6874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5881 809
Size: 507 4565 1802
Fragments arranged by size:
4565 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 2598 3267
Size: 54 1425 50 275 18 723 45 669
Cuts at: 3267 3828 4075 4321 5210 6839 8
Size: 561 247 246 889 1629 43
Fragments arranged by size:
1629 1425 889 723 669 561 275 247
246 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4321 1537
Size: 275 2509 4090
Fragments arranged by size:
4090 2509 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2771 3579 3699 4804 1353
Size: 665 753 808 120 1105 3423
Fragments arranged by size:
3423 1105 808 753 665 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3154 5461 5835 5981 387
Size: 1064 138 1565 2307 374 146 1280
Fragments arranged by size:
2307 1565 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 3589 2125
Size: 1464 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2306 2375 2441 2549 2693 2819
Size: 393 446 228 69 66 108 144 126
Cuts at: 2819 2843 2876 2942 3008 3368 3488 3533 3758
Size: 24 33 66 66 360 120 45 225
Cuts at: 3758 4028 4937 5534 6321 6589 1239
Size: 270 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 597 446 393 360 270
268 228 225 144 126 120 108 69
66 66 66 45 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3737 3830 3737
Size: 93 6781
EcoO109I rG'GnC_Cy
Cuts at: 2929 3532 3823 2929
Size: 603 291 5980
Fragments arranged by size:
5980 603 291
EcoRI G'AATT_C
Cuts at: 4183 4561 4183
Size: 378 6496
FspI TGC'GCA
Cuts at: 3777 4115 5380 3777
Size: 338 1265 5271
Fragments arranged by size:
5271 1265 338
HaeII r_GCGC'y
Cuts at: 1678 2824 2950 3529 3721 4153 6021 1678
Size: 1146 126 579 192 432 1868 2531
Fragments arranged by size:
2531 1868 1146 579 432 192 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 2714 2746 2897
Size: 32 135 32 925 32 185 32 151
Cuts at: 2897 2929 3146 3178 3482 3514 3643 3675 3731
Size: 32 217 32 304 32 129 32 56
Cuts at: 3731 3763 5515 5547 5589 5621 1373
Size: 32 1752 32 42 32 2626
Fragments arranged by size:
2626 1752 925 304 217 185 151 135
129 56 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4218 4464 669
Size: 988 281 2280 246 3079
Fragments arranged by size:
3079 2280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KasI G'GCGC_C
Cuts at: 2820 2820
Size: 6874
KpnI G_GTAC'C
Cuts at: 4199 4199
Size: 6874
MfeI C'AATT_G
Cuts at: 4473 4473
Size: 6874
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5988 6172 1305
Size: 4683 184 2007
Fragments arranged by size:
4683 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3830 4077 10
Size: 54 1425 343 1998 247 2807
Fragments arranged by size:
2807 1998 1425 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3212 4832 5191 5350 518
Size: 648 2046 1620 359 159 2042
Fragments arranged by size:
2046 2042 1620 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2311 2572 2839 2986 3457 3466 3556
Size: 675 30 261 267 147 471 9 90
Cuts at: 3556 3724 4126 4290 4967 6115 6360 1606
Size: 168 402 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 471 402 267 261
245 168 164 147 90 30 9
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 6874
NarI GG'CG_CC
Cuts at: 2821 2821
Size: 6874
NcoI C'CATG_G
Cuts at: 513 1065 2556 513
Size: 552 1491 4831
Fragments arranged by size:
4831 1491 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 6874
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6874
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NsiI A_TGCA'T
Cuts at: 3408 3408
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 2160 4232 5777 1132
Size: 97 931 2072 1545 2229
Fragments arranged by size:
2229 2072 1545 931 97
PasI CC'CwG_GG
Cuts at: 1348 3818 1348
Size: 2470 4404
PciI A'CATG_T
Cuts at: 5773 5773
Size: 6874
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3727 1108
Size: 2619 4255
PfoI T'CCnGG_A
Cuts at: 3504 3591 3735 3882 3504
Size: 87 144 147 6496
Fragments arranged by size:
6496 147 144 87
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 4943 4975 6456 6488 2747
Size: 32 2164 32 1481 32 3133
Fragments arranged by size:
3133 2164 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3823 3823
Size: 6874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 4444 4444
Size: 6874
PspOMI G'GGCC_C
Cuts at: 2714 3531 2714
Size: 817 6057
PspXI vC'TCGA_Gb
Cuts at: 2807 2807
Size: 6874
PstI C_TGCA'G
Cuts at: 830 2172 2231 2979 3036 3348 3861 4003 4226
Size: 1342 59 748 57 312 513 142 223
Cuts at: 4226 830
Size: 3478
Fragments arranged by size:
3478 1342 748 513 312 223 142 59
57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 2281 2311 2986 3457 3466 3556 3724 2281
Size: 30 675 471 9 90 168 5431
Fragments arranged by size:
5431 675 471 168 90 30 9
SacI G_AGCT'C
Cuts at: 2127 3591 2127
Size: 1464 5410
SacII CC_GC'GG
Cuts at: 2573 2573
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 3579 2018
Size: 1561 5313
SbfI CC_TGCA'GG
Cuts at: 3348 4226 3348
Size: 878 5996
ScaI AGT'ACT
Cuts at: 2657 5122 6834 2657
Size: 2465 1712 2697
Fragments arranged by size:
2697 2465 1712
SfcI C'TryA_G
Cuts at: 826 1045 2168 2227 2975 3032 3344 3857 3999
Size: 219 1123 59 748 57 312 513 142
Cuts at: 3999 4222 5357 6038 6229 826
Size: 223 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 748 681 513 312 223
219 191 142 59 57
SfoI GGC'GCC
Cuts at: 2822 2822
Size: 6874
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 4201 1872
Size: 2329 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2731 2807 4978 5879 6141 820
Size: 197 926 788 76 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 788 262 197 76
SnaBI TAC'GTA
Cuts at: 493 4665 493
Size: 4172 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6874
SphI G_CATG'C
Cuts at: 1132 4232 1132
Size: 3100 3774
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 3254 4019 1517
Size: 1737 765 4372
Fragments arranged by size:
4372 1737 765
StyI C'CwwG_G
Cuts at: 513 1065 2556 3831 4313 513
Size: 552 1491 1275 482 3074
Fragments arranged by size:
3074 1491 1275 552 482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2864 3923 4901 4918 5071 5256 5701
Size: 1255 1284 1059 978 17 153 185 445
Cuts at: 5701 325
Size: 1498
Fragments arranged by size:
1498 1284 1255 1059 978 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 5120 6832
Size: 274 80 33 51 1443 678 2465 1712
Cuts at: 6832 96
Size: 138
Fragments arranged by size:
2465 1712 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 2192 2224 2732 2764 6834 6866 2020
Size: 32 140 32 508 32 4070 32 2028
Fragments arranged by size:
4070 2028 508 140 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6874
XbaI T'CTAG_A
Cuts at: 2260 4210 2260
Size: 1950 4924
XhoI C'TCGA_G
Cuts at: 1943 2807 1943
Size: 864 6010
XmaI C'CCGG_G
Cuts at: 1870 4199 1870
Size: 2329 4545
XmnI GAAnn'nnTTC
Cuts at: 2759 5003 2759
Size: 2244 4630
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI NruI PacI PmlI
PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI