Restriction map for
pFN21AB3292
(Circular) (Six-base) MAPSORT of: pFN21AB3292.seq Check: 9345 from: 1 to: 6382
pFN21AB3292 6382 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3243 3703 3243
Size: 460 5922
AclI AA'CG_TT
Cuts at: 1563 2560 4509 4882 1563
Size: 997 1949 373 3063
Fragments arranged by size:
3063 1949 997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2071 4445 5829 6097 1632
Size: 439 2374 1384 268 1917
Fragments arranged by size:
2374 1917 1384 439 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 3025 3034 3060 5281 6258 1203
Size: 1822 9 26 2221 977 1327
Fragments arranged by size:
2221 1822 1327 977 26 9
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2417 1184
Size: 1233 5149
AlwNI CAG_nnn'CTG
Cuts at: 2759 3574 4125 5697 2759
Size: 815 551 1572 3444
Fragments arranged by size:
3444 1572 815 551
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2012 2970 3131 3139 3273 3369 3691 3917
Size: 519 958 161 8 134 96 322 226
Cuts at: 3917 4069 6178 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 958 519 322 226 161 152
134 96 8
AseI AT'TA_AT
Cuts at: 160 1032 2424 4936 160
Size: 872 1392 2512 1606
Fragments arranged by size:
2512 1606 1392 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 3712 4079 3712
Size: 367 6015
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3243 3604 3703 5158 618
Size: 325 217 627 1456 361 99 1455 1842
Fragments arranged by size:
1842 1456 1455 627 361 325 217 99
BanII G_rGCy'C
Cuts at: 1391 3701 1391
Size: 2310 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2346 2817 4274 5484 2346
Size: 471 1457 1210 3244
Fragments arranged by size:
3244 1457 1210 471
BclI T'GATC_A
Cuts at: 1548 2571 2616 2724 1548
Size: 1023 45 108 5206
Fragments arranged by size:
5206 1023 108 45
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4993 136
Size: 107 122 71 631 605 3321 1525
Fragments arranged by size:
3321 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2291 6377 2291
Size: 4086 2296
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 3609 4443 5599 1790
Size: 1819 834 1156 2573
Fragments arranged by size:
2573 1819 1156 834
BmgBI CAC'GTC
Cuts at: 1318 3281 1318
Size: 1963 4419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3470 3527 4113
Size: 416 53 83 399 24 2053 57 586
Cuts at: 4113 5071 442
Size: 958 1753
Fragments arranged by size:
2053 1753 958 586 416 399 83 57
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2199 2681 3144 5042 1239
Size: 918 42 482 463 1898 2579
Fragments arranged by size:
2579 1898 918 482 463 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4507 5372 5670 4507
Size: 865 298 5219
Fragments arranged by size:
5219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5045 882
Size: 617 3546 2219
Fragments arranged by size:
3546 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4189 4571 5206 275
Size: 53 83 186 936 2656 382 635 1451
Fragments arranged by size:
2656 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4815 5213 5487 5634 6045 4815
Size: 398 274 147 411 5152
Fragments arranged by size:
5152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 1178
Size: 30 165 30 1180 30 4947
Fragments arranged by size:
4947 1180 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2970 1186
Size: 1784 4598
BseYI C'CCAG_C
Cuts at: 3608 3639 5585 6081 3608
Size: 31 1946 496 3909
Fragments arranged by size:
3909 1946 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5809
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2396 4443 4528 5599 2396
Size: 2047 85 1071 3179
Fragments arranged by size:
3179 2047 1071 85
BsmI GAATG_Cn'
Cuts at: 2099 3893 3986 2099
Size: 1794 93 4495
Fragments arranged by size:
4495 1794 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 3609 3701 4443 4528 5599 1391
Size: 399 606 1213 92 742 85 1071 2174
Fragments arranged by size:
2174 1213 1071 742 606 399 92 85
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 2167 4271 6001 2167
Size: 2104 1730 2548
Fragments arranged by size:
2548 2104 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3737 844
Size: 2893 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3631 4877 5051 65
Size: 1183 2383 1246 174 1396
Fragments arranged by size:
2383 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5026 5213 1700
Size: 110 3216 187 2869
Fragments arranged by size:
3216 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2645 96
Size: 2549 3833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 2548 3655 5186 2548
Size: 1107 1531 3744
Fragments arranged by size:
3744 1531 1107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2291 3712 4079 4464 4481 5933 6019 6031
Size: 319 1421 367 385 17 1452 86 12
Cuts at: 6031 6377 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1421 385 367 346 319 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2874 3382 513
Size: 552 1809 508 3513
Fragments arranged by size:
3513 1809 552 508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5643
Fragments arranged by size:
5643 683 56
BtsI GCAGTG_nn'
Cuts at: 2996 3650 3898 4692 4712 2996
Size: 654 248 794 20 4666
Fragments arranged by size:
4666 794 654 248 20
ClaI AT'CG_AT
Cuts at: 1554 2718 1554
Size: 1164 5218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2343 2378 529
Size: 35 1779 35 4533
Fragments arranged by size:
4533 1779 35 35
DraI TTT'AAA
Cuts at: 2242 3686 2242
Size: 1444 4938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5389 809
Size: 507 4073 1802
Fragments arranged by size:
4073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3829 4718
Size: 54 1425 50 275 18 843 1156 889
Cuts at: 4718 6347 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1156 889 843 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2859 3585 4312 1353
Size: 1506 726 727 3423
Fragments arranged by size:
3423 1506 727 726
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2157 2199 2681 3144 4445 5042
Size: 393 439 86 42 482 463 1301 597
Cuts at: 5042 5829 6097 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1301 787 597 482 463 439 393
268 86 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2169 2169
Size: 6382
EcoO109I rG'GnC_Cy
Cuts at: 2174 2174
Size: 6382
EcoRI G'AATT_C
Cuts at: 3131 3691 4069 3131
Size: 560 378 5444
Fragments arranged by size:
5444 560 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 6382
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 5529 1678
Size: 3851 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3179 3211 5023 5055 5097
Size: 32 135 32 1607 32 1812 32 42
Cuts at: 5097 5129 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 1607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6382
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3247 3707 3247
Size: 460 5922
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3010 3346 5496 5680 1305
Size: 1705 336 2150 184 2007
Fragments arranged by size:
2150 2007 1705 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 3717
Fragments arranged by size:
3717 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 4340 4699 4858 518
Size: 648 1554 1620 359 159 2042
Fragments arranged by size:
2042 1620 1554 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3798 4475 5623 5868 1606
Size: 2192 677 1148 245 2120
Fragments arranged by size:
2192 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2874 3382 513
Size: 552 1809 508 3513
Fragments arranged by size:
3513 1809 552 508
NdeI CA'TA_TG
Cuts at: 387 2929 387
Size: 2542 3840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3021 3029 3038 3557 3740 5285 1132
Size: 97 1792 8 9 519 183 1545 2229
Fragments arranged by size:
2229 1792 1545 519 183 97 9 8
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 3025 3034 5281 3025
Size: 9 2247 4126
Fragments arranged by size:
4126 2247 9
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3388 1108
Size: 2280 4102
PfoI T'CCnGG_A
Cuts at: 2176 2625 3067 2176
Size: 449 442 5491
Fragments arranged by size:
5491 449 442
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2174 2174
Size: 6382
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PstI C_TGCA'G
Cuts at: 830 3552 3734 830
Size: 2722 182 3478
Fragments arranged by size:
3478 2722 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SfcI C'TryA_G
Cuts at: 826 1045 3548 3730 4865 5546 5737 826
Size: 219 2503 182 1135 681 191 1471
Fragments arranged by size:
2503 1471 1135 681 219 191 182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4486 5387 5649 820
Size: 197 926 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 3678 4306 5
Size: 47 3626 628 2081
Fragments arranged by size:
3626 2081 628 47
StuI AGG'CCT
Cuts at: 1517 2500 2886 1517
Size: 983 386 5013
Fragments arranged by size:
5013 983 386
StyI C'CwwG_G
Cuts at: 513 1065 2874 3382 3821 513
Size: 552 1809 508 439 3074
Fragments arranged by size:
3074 1809 552 508 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3624 4409 4426 4579 4764 5209 325
Size: 1255 2044 785 17 153 185 445 1498
Fragments arranged by size:
2044 1498 1255 785 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 2645 2745
Size: 274 80 33 51 1443 178 490 100
Cuts at: 2745 4628 6340 96
Size: 1883 1712 138
Fragments arranged by size:
1883 1712 1443 490 274 178 138 100
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 6342 6374 2583
Size: 32 3727 32 2591
Fragments arranged by size:
3727 2591 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 2146 3331 3718 2146
Size: 1185 387 4810
Fragments arranged by size:
4810 1185 387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 2457 3143 4511 2457
Size: 686 1368 4328
Fragments arranged by size:
4328 1368 686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI