Restriction map for
pFN21AB3289
(Circular) (Six-base) MAPSORT of: pFN21AB3289.seq Check: 4530 from: 1 to: 7114
pFN21AB3289 7114 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4457 1937
Size: 2520 4594
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2770 3103 3122 3187 3374 3523 3607
Size: 822 316 333 19 65 187 149 84
Cuts at: 3607 3710 3859 4111 4195 4279 5177 6561 6829
Size: 103 149 252 84 84 898 1384 268
Cuts at: 6829 1632
Size: 1917
Fragments arranged by size:
1917 1384 898 822 333 316 268 252
187 149 149 103 84 84 84 65
19
AflII C'TTAA_G
Cuts at: 820 1017 2924 820
Size: 197 1907 5010
Fragments arranged by size:
5010 1907 197
AflIII A'CryG_T
Cuts at: 1203 2846 2908 6013 6990 1203
Size: 1643 62 3105 977 1327
Fragments arranged by size:
3105 1643 1327 977 62
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 2661 1166
Size: 1495 5619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2340 1184
Size: 1156 5958
AlwNI CAG_nnn'CTG
Cuts at: 4857 6429 4857
Size: 1572 5542
ApaI G_GGCC'C
Cuts at: 2084 2084
Size: 7114
ApaLI G'TGCA_C
Cuts at: 3969 4079 5171 6327 3969
Size: 110 1092 1156 4756
Fragments arranged by size:
4756 1156 1092 110
ApoI r'AATT_y
Cuts at: 1493 2233 2427 2571 2948 3211 3547 3813 4423
Size: 740 194 144 377 263 336 266 610
Cuts at: 4423 4649 4801 6910 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 610 377 336 266 263
226 194 152 144
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 4439 1870
Size: 73 2496 4545
Fragments arranged by size:
4545 2496 73
BamHI G'GATC_C
Cuts at: 3825 4444 4811 3825
Size: 619 367 6128
Fragments arranged by size:
6128 619 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4435 5890 618
Size: 325 217 627 2648 1455 1842
Fragments arranged by size:
2648 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 3719 4433 1391
Size: 693 1635 714 4072
Fragments arranged by size:
4072 1635 714 693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 3380 3865 4136 4201 4212 928
Size: 971 331 1150 485 271 65 11 3830
Fragments arranged by size:
3830 1150 971 485 331 271 65 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 2358 2722 1548
Size: 810 364 5940
Fragments arranged by size:
5940 810 364
BfrBI ATG'CAT
Cuts at: 3188 4069 3188
Size: 881 6233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5725 136
Size: 107 122 71 631 605 4053 1525
Fragments arranged by size:
4053 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3805 7109 3805
Size: 3304 3810
BlpI GC'TnA_GC
Cuts at: 4531 4531
Size: 7114
Bme1580I G_kGCm'C
Cuts at: 1790 2084 3973 4083 5175 6331 1790
Size: 294 1889 110 1092 1156 2573
Fragments arranged by size:
2573 1889 1156 1092 294 110
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2490 2876 3171
Size: 416 53 83 399 24 1073 386 295
Cuts at: 3171 4263 4845 5803 442
Size: 1092 582 958 1753
Fragments arranged by size:
1753 1092 1073 958 582 416 399 386
295 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2782 3435 3519 4023 5774 1239
Size: 1543 653 84 504 1751 2579
Fragments arranged by size:
2579 1751 1543 653 504 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2524 4123 5239 6104 6402 2524
Size: 1599 1116 865 298 3236
Fragments arranged by size:
3236 1599 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5777 882
Size: 617 4278 2219
Fragments arranged by size:
4278 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2992 4905 493
Size: 711 1788 1913 2702
Fragments arranged by size:
2702 1913 1788 711
BsaBI GATnn'nnATC
Cuts at: 2811 7108 2811
Size: 4297 2817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4921 5303 5938 275
Size: 53 83 186 936 3388 382 635 1451
Fragments arranged by size:
3388 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5547 5945 6219 6366 6777 5547
Size: 398 274 147 411 5884
Fragments arranged by size:
5884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6889
Fragments arranged by size:
6889 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 3116 3284 3704 3872 1186
Size: 889 1041 168 420 168 4428
Fragments arranged by size:
4428 1041 889 420 168 168
BseYI C'CCAG_C
Cuts at: 2077 6317 6813 2077
Size: 4240 496 2378
Fragments arranged by size:
4240 2378 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3964 4564 5325 6353 1540
Size: 275 186 1963 600 761 1028 2301
Fragments arranged by size:
2301 1963 1028 761 600 275 186
BsiHKAI G_wGCw'C
Cuts at: 3719 3973 4083 5175 5260 6331 3719
Size: 254 110 1092 85 1071 4502
Fragments arranged by size:
4502 1092 1071 254 110 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 3719 3973 4083 4433 5175 5260
Size: 399 294 1635 254 110 350 742 85
Cuts at: 5260 6331 1391
Size: 1071 2174
Fragments arranged by size:
2174 1635 1071 742 399 350 294 254
110 85
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 2638 5003 6733 2638
Size: 2365 1730 3019
Fragments arranged by size:
3019 2365 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 4469 844
Size: 1298 2327 3489
Fragments arranged by size:
3489 2327 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 5001 1394
Size: 429 3178 3507
Fragments arranged by size:
3507 3178 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3026 3061 3526 5609 5783 65
Size: 1183 1778 35 465 2083 174 1396
Fragments arranged by size:
2083 1778 1396 1183 465 174 35
BsrFI r'CCGG_y
Cuts at: 1700 1810 5758 5945 1700
Size: 110 3948 187 2869
Fragments arranged by size:
3948 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 5174 6186 5174
Size: 1012 6102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7114
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstYI r'GATC_y
Cuts at: 1972 3805 3825 4444 4811 5196 5213 6665 6751
Size: 1833 20 619 367 385 17 1452 86
Cuts at: 6751 6763 7109 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1833 1452 619 385 367 346 86
20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6375
Fragments arranged by size:
6375 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 4630 5424 5444 2031
Size: 2599 794 20 3701
Fragments arranged by size:
3701 2599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3508 3543 529
Size: 35 2944 35 4100
Fragments arranged by size:
4100 2944 35 35
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2416 6121 809
Size: 507 1100 3705 1802
Fragments arranged by size:
3705 1802 1100 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4561 5450 7079
Size: 54 1425 50 275 18 2731 889 1629
Cuts at: 7079 8
Size: 43
Fragments arranged by size:
2731 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4561 1537
Size: 275 2749 4090
Fragments arranged by size:
4090 2749 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 3527 3768 3947 5044 1353
Size: 933 1241 241 179 1097 3423
Fragments arranged by size:
3423 1241 1097 933 241 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5701 6075 6221 387
Size: 1064 138 4112 374 146 1280
Fragments arranged by size:
4112 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3717 3717
Size: 7114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2770 2782 3103 3122 3187 3374
Size: 393 822 316 12 321 19 65 187
Cuts at: 3374 3435 3519 3523 3607 3710 3859 4023 4111
Size: 61 84 4 84 103 149 164 88
Cuts at: 4111 4195 4279 5177 5774 6561 6829 1239
Size: 84 84 898 597 787 268 1524
Fragments arranged by size:
1524 898 822 787 597 393 321 316
268 187 164 149 103 88 84 84
84 84 65 61 19 12 4
EcoO109I rG'GnC_Cy
Cuts at: 2081 3114 2081
Size: 1033 6081
EcoRI G'AATT_C
Cuts at: 2948 3211 3547 4423 4801 2948
Size: 263 336 876 378 5261
Fragments arranged by size:
5261 876 378 336 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2312 4069 2312
Size: 1757 5357
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2964 6261 1678
Size: 1286 3297 2531
Fragments arranged by size:
3297 2531 1286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 2620 2652 2707
Size: 32 135 32 695 32 321 32 55
Cuts at: 2707 2739 3190 3222 3454 3486 3946 3958 3978
Size: 32 451 32 232 32 460 12 20
Cuts at: 3978 3990 5755 5787 5829 5861 1373
Size: 12 1765 32 42 32 2626
Fragments arranged by size:
2626 1765 695 460 451 321 232 135
55 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4458 4704 669
Size: 988 281 2520 246 3079
Fragments arranged by size:
3079 2520 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7114
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2606 3188 3944 6228 6412 1305
Size: 1301 582 756 2284 184 2007
Fragments arranged by size:
2284 2007 1301 756 582 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5292
Fragments arranged by size:
5292 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2640 2661 3010 3178 3262 3430
Size: 648 843 631 21 349 168 84 168
Cuts at: 3430 3514 3750 3766 3934 4018 4102 4158 4186
Size: 84 236 16 168 84 84 56 28
Cuts at: 4186 4338 5072 5431 5590 518
Size: 152 734 359 159 2042
Fragments arranged by size:
2042 843 734 648 631 359 349 236
168 168 168 159 152 84 84 84
84 56 28 21 16
MspA1I CmG'CkG
Cuts at: 1606 3739 4530 5207 6355 6600 1606
Size: 2133 791 677 1148 245 2120
Fragments arranged by size:
2133 2120 1148 791 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6562
NdeI CA'TA_TG
Cuts at: 387 3431 387
Size: 3044 4070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NsiI A_TGCA'T
Cuts at: 3190 4071 3190
Size: 881 6233
NspI r_CATG'y
Cuts at: 1132 1229 2850 2912 3192 4069 4472 6017 1132
Size: 97 1621 62 280 877 403 1545 2229
Fragments arranged by size:
2229 1621 1545 877 403 280 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7114
PciI A'CATG_T
Cuts at: 2846 2908 6013 2846
Size: 62 3105 3947
Fragments arranged by size:
3947 3105 62
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2625 1108
Size: 1517 5597
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PmlI CAC'GTG
Cuts at: 2992 2992
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7114
PsiI TTA'TAA
Cuts at: 3093 4269 4684 3093
Size: 1176 415 5523
Fragments arranged by size:
5523 1176 415
PspOMI G'GGCC_C
Cuts at: 2080 2080
Size: 7114
PstI C_TGCA'G
Cuts at: 830 4466 830
Size: 3636 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 3739 3739
Size: 7114
SacI G_AGCT'C
Cuts at: 3719 3719
Size: 7114
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SapI GCTCTTCn'nnn_
Cuts at: 2286 3527 2286
Size: 1241 5873
SbfI CC_TGCA'GG
Cuts at: 4466 4466
Size: 7114
ScaI AGT'ACT
Cuts at: 4124 5362 7074 4124
Size: 1238 1712 4164
Fragments arranged by size:
4164 1712 1238
SexAI A'CCwGG_T
Cuts at: 2339 2400 2339
Size: 61 7053
SfcI C'TryA_G
Cuts at: 826 1045 2178 2969 3820 4229 4462 5597 6278
Size: 219 1133 791 851 409 233 1135 681
Cuts at: 6278 6469 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1133 851 791 681 409 233
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 4441 1872
Size: 2569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2503 2924 4138 5218 6119 6381
Size: 197 926 560 421 1214 1080 901 262
Cuts at: 6381 820
Size: 1553
Fragments arranged by size:
1553 1214 1080 926 901 560 421 262
197
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 4472 1132
Size: 3340 3774
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 3128 3884 4136 1517
Size: 1611 756 252 4495
Fragments arranged by size:
4495 1611 756 252
StyI C'CwwG_G
Cuts at: 513 1065 4553 513
Size: 552 3488 3074
Fragments arranged by size:
3488 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 4206 5141 5158 5311 5496 5941
Size: 1255 2370 256 935 17 153 185 445
Cuts at: 5941 325
Size: 1498
Fragments arranged by size:
2370 1498 1255 935 445 256 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2653 2905 3434
Size: 274 80 33 51 1443 676 252 529
Cuts at: 3434 4122 5360 7072 96
Size: 688 1238 1712 138
Fragments arranged by size:
1712 1443 1238 688 676 529 274 252
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7074 7106 7074
Size: 32 7082
Tth111I GACn'n_nGTC
Cuts at: 1316 2542 1316
Size: 1226 5888
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 4439 1870
Size: 2569 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 2531 3032 3173 3536 3620 3872 3956 4040
Size: 519 501 141 363 84 252 84 84
Cuts at: 4040 5243 2012
Size: 1203 3883
Fragments arranged by size:
3883 1203 519 501 363 252 141 84
84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI