Restriction map for
pFN21AB3287
(Circular) (Six-base) MAPSORT of: pFN21AB3287.seq Check: 9392 from: 1 to: 7573
pFN21AB3287 7573 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4916 1937
Size: 2979 4594
Acc65I G'GTAC_C
Cuts at: 4894 4894
Size: 7573
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3317 4361 5636 7020 7288 1632
Size: 1079 606 1044 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1275 1079 1044 606 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 4083 6472 7449 1203
Size: 2880 2389 977 1327
Fragments arranged by size:
2880 2389 1327 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 2569 6302 2569
Size: 3733 3840
AleI CACnn'nnGTG
Cuts at: 1166 3738 1166
Size: 2572 5001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3971 1184
Size: 2787 4786
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 7541
AlwNI CAG_nnn'CTG
Cuts at: 2968 3795 4717 4795 5316 6888 2968
Size: 827 922 78 521 1572 3653
Fragments arranged by size:
3653 1572 922 827 521 78
ApaLI G'TGCA_C
Cuts at: 5630 6786 5630
Size: 1156 6417
ApoI r'AATT_y
Cuts at: 1493 3095 4045 4147 4882 5108 5260 7369 1493
Size: 1602 950 102 735 226 152 2109 1697
Fragments arranged by size:
2109 1697 1602 950 735 226 152 102
AscI GG'CGCG_CC
Cuts at: 4597 4597
Size: 7573
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 3431 4631 4772 4868 4898 1870
Size: 73 652 836 1200 141 96 30 4545
Fragments arranged by size:
4545 1200 836 652 141 96 73 30
BamHI G'GATC_C
Cuts at: 2460 4903 5270 2460
Size: 2443 367 4763
Fragments arranged by size:
4763 2443 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 3035 3443 4894 6349
Size: 325 217 627 233 1015 408 1451 1455
Cuts at: 6349 618
Size: 1842
Fragments arranged by size:
1842 1455 1451 1015 627 408 325 233
217
BanII G_rGCy'C
Cuts at: 1391 3830 4698 4869 4892 1391
Size: 2439 868 171 23 4072
Fragments arranged by size:
4072 2439 868 171 23
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 7573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6602
BbvCI CC'TCA_GC
Cuts at: 3197 3197
Size: 7573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 4791 4825 5764
Size: 34 50 34 2228 34 508 34 939
Cuts at: 5764 5798 1903
Size: 34 3678
Fragments arranged by size:
3678 2228 939 508 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4589 5465 6675 4589
Size: 876 1210 5487
Fragments arranged by size:
5487 1210 876
BclI T'GATC_A
Cuts at: 1548 3894 1548
Size: 2346 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3671 3923 4618
Size: 107 122 71 631 605 1999 252 695
Cuts at: 4618 4739 6184 136
Size: 121 1445 1525
Fragments arranged by size:
1999 1525 1445 695 631 605 252 122
121 107 71
BglII A'GATC_T
Cuts at: 3021 3487 7568 3021
Size: 466 4081 3026
Fragments arranged by size:
4081 3026 466
BlpI GC'TnA_GC
Cuts at: 4664 4990 4664
Size: 326 7247
Bme1580I G_kGCm'C
Cuts at: 1790 2895 3745 3960 4373 4655 4804 5634 6790
Size: 1105 850 215 413 282 149 830 1156
Cuts at: 6790 1790
Size: 2573
Fragments arranged by size:
2573 1156 1105 850 830 413 282 215
149
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2910 5304 6262
Size: 416 53 83 399 24 1493 2394 958
Cuts at: 6262 442
Size: 1753
Fragments arranged by size:
2394 1753 1493 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2296 2744 2768 4009 4292 4352 4856 6233
Size: 1057 448 24 1241 283 60 504 1377
Cuts at: 6233 1239
Size: 2579
Fragments arranged by size:
2579 1377 1241 1057 504 448 283 60
24
Bpu10I CC'TnA_GC
Cuts at: 3197 3197
Size: 7573
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2098 2600 3344 5698 6563 6861 2098
Size: 502 744 2354 865 298 2810
Fragments arranged by size:
2810 2354 865 744 502 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6236 882
Size: 617 4737 2219
Fragments arranged by size:
4737 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3184 5364 493
Size: 711 1980 2180 2702
Fragments arranged by size:
2702 2180 1980 711
BsaBI GATnn'nnATC
Cuts at: 3899 7567 3899
Size: 3668 3905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3642 3710 3873
Size: 53 83 186 936 488 1621 68 163
Cuts at: 3873 5380 5762 6397 275
Size: 1507 382 635 1451
Fragments arranged by size:
1621 1507 1451 936 635 488 382 186
163 83 68 53
BsaWI w'CCGG_w
Cuts at: 2973 3802 6006 6404 6678 6825 7236 2973
Size: 829 2204 398 274 147 411 3310
Fragments arranged by size:
3310 2204 829 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3761 3791 4007 4037 1178
Size: 30 165 30 2358 30 216 30 4714
Fragments arranged by size:
4714 2358 216 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2603 2905 4139 4649 4841 1186
Size: 1204 213 302 1234 510 192 3918
Fragments arranged by size:
3918 1234 1204 510 302 213 192
BseYI C'CCAG_C
Cuts at: 2179 2305 2426 2456 4100 6776 7272 2179
Size: 126 121 30 1644 2676 496 2480
Fragments arranged by size:
2676 2480 1644 496 126 121 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 2464 2981 3962 4735 1163
Size: 573 398 330 517 981 773 4001
Fragments arranged by size:
4001 981 773 573 517 398 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3807 4646 4808 5023 5784 6812
Size: 275 186 1806 839 162 215 761 1028
Cuts at: 6812 1540
Size: 2301
Fragments arranged by size:
2301 1806 1028 839 761 275 215 186
162
BsiHKAI G_wGCw'C
Cuts at: 4121 5634 5719 6790 4121
Size: 1513 85 1071 4904
Fragments arranged by size:
4904 1513 1071 85
BsmI GAATG_Cn'
Cuts at: 2519 3573 3884 5084 5177 2519
Size: 1054 311 1200 93 4915
Fragments arranged by size:
4915 1200 1054 311 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2020
Size: 7573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2895 3745 3830 3960 4121 4373 4655
Size: 399 1105 850 85 130 161 252 282
Cuts at: 4655 4698 4804 4869 4892 5634 5719 6790 1391
Size: 43 106 65 23 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 850 742 399 282 252
161 130 106 85 85 65 43 23
BspEI T'CCGG_A
Cuts at: 7236 7236
Size: 7573
BspHI T'CATG_A
Cuts at: 3045 3555 5462 7192 3045
Size: 510 1907 1730 3426
Fragments arranged by size:
3426 1907 1730 510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3063 4928 844
Size: 2219 1865 3489
Fragments arranged by size:
3489 2219 1865
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 2611 5460 1394
Size: 429 305 483 2849 3507
Fragments arranged by size:
3507 2849 483 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3331 6068 6242 65
Size: 1183 2083 2737 174 1396
Fragments arranged by size:
2737 2083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3083 3750 4590 6217 6404 1700
Size: 110 1273 667 840 1627 187 2869
Fragments arranged by size:
2869 1627 1273 840 667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 4597 1925
Size: 2672 4901
BssSI C'ACGA_G
Cuts at: 2048 5633 6645 2048
Size: 3585 1012 2976
Fragments arranged by size:
3585 2976 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7573
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 4540 1184
Size: 3356 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2275 3274 4468 2143
Size: 132 999 1194 5248
Fragments arranged by size:
5248 1194 999 132
BstYI r'GATC_y
Cuts at: 1972 2259 2460 2622 3021 3375 3396 3487 4335
Size: 287 201 162 399 354 21 91 848
Cuts at: 4335 4903 5270 5655 5672 7124 7210 7222 7568
Size: 568 367 385 17 1452 86 12 346
Cuts at: 7568 1972
Size: 1977
Fragments arranged by size:
1977 1452 848 568 399 385 367 354
346 287 201 162 91 86 21 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2106 4646 4744 513
Size: 552 1041 2540 98 3342
Fragments arranged by size:
3342 2540 1041 552 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3820 487
Size: 683 56 2594 4240
Fragments arranged by size:
4240 2594 683 56
BtsI GCAGTG_nn'
Cuts at: 5089 5883 5903 5089
Size: 794 20 6759
Fragments arranged by size:
6759 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2317 2352 4055 4090 529
Size: 35 1753 35 1703 35 4012
Fragments arranged by size:
4012 1753 1703 35 35 35
DraI TTT'AAA
Cuts at: 2523 4877 2523
Size: 2354 5219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 3085 3172
Size: 54 1425 50 275 18 315 940 87
Cuts at: 3172 3672 3915 4299 4458 4805 5020 5909 7538
Size: 500 243 384 159 347 215 889 1629
Cuts at: 7538 8
Size: 43
Fragments arranged by size:
1629 1425 940 889 500 384 347 315
275 243 215 159 87 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4805 5020 1537
Size: 275 2993 215 4090
Fragments arranged by size:
4090 2993 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 3248 3656 3695 4109 5503 1353
Size: 1895 408 39 414 1394 3423
Fragments arranged by size:
3423 1895 1394 414 408 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3389 6160 6534 6680 387
Size: 1064 138 1800 2771 374 146 1280
Fragments arranged by size:
2771 1800 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2296 2711 2744 2768 3317 4009 4292
Size: 393 664 415 33 24 549 692 283
Cuts at: 4292 4352 4361 4856 5636 6233 7020 7288 1239
Size: 60 9 495 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 692 664 597 549 495
415 393 283 268 60 33 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2690 4568 2267
Size: 423 1878 5272
Fragments arranged by size:
5272 1878 423
EcoO109I rG'GnC_Cy
Cuts at: 2350 2599 3193 4228 4792 2350
Size: 249 594 1035 564 5131
Fragments arranged by size:
5131 1035 594 564 249
EcoRI G'AATT_C
Cuts at: 3095 4147 4882 5260 3095
Size: 1052 735 378 5408
Fragments arranged by size:
5408 1052 735 378
FspI TGC'GCA
Cuts at: 3059 4553 6079 3059
Size: 1494 1526 4553
Fragments arranged by size:
4553 1526 1494
HaeII r_GCGC'y
Cuts at: 1678 2024 4642 6720 1678
Size: 346 2618 2078 2531
Fragments arranged by size:
2618 2531 2078 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 6214 6246 6288
Size: 32 135 32 983 32 3627 32 42
Cuts at: 6288 6320 1373
Size: 32 2626
Fragments arranged by size:
3627 2626 983 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2794 3708 4917 5163 669
Size: 988 281 856 914 1209 246 3079
Fragments arranged by size:
3079 1209 988 914 856 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 7573
KpnI G_GTAC'C
Cuts at: 4898 4898
Size: 7573
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3144 3249 4176 4458 6687 6871 1305
Size: 1839 105 927 282 2229 184 2007
Fragments arranged by size:
2229 2007 1839 927 282 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 4301 4460 10
Size: 54 1425 343 315 2154 159 3123
Fragments arranged by size:
3123 2154 1425 343 315 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3216 3736 3738 3953 4466 5531
Size: 648 1278 772 520 2 215 513 1065
Cuts at: 5531 5890 6049 518
Size: 359 159 2042
Fragments arranged by size:
2042 1278 1065 772 648 520 513 359
215 159 2
MspA1I CmG'CkG
Cuts at: 1606 3518 3681 3861 4312 4648 4746 4989 5666
Size: 1912 163 180 451 336 98 243 677
Cuts at: 5666 6814 7059 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1912 1148 677 451 336 245 243
180 163 98
NaeI GCC'GGC
Cuts at: 4592 4592
Size: 7573
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 2106 513
Size: 552 1041 5980
Fragments arranged by size:
5980 1041 552
NdeI CA'TA_TG
Cuts at: 387 3591 387
Size: 3204 4369
NgoMIV G'CCGG_C
Cuts at: 4590 4590
Size: 7573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2584 3358 4075 4087 4264 4931 6476
Size: 97 1355 774 717 12 177 667 1545
Cuts at: 6476 1132
Size: 2229
Fragments arranged by size:
2229 1545 1355 774 717 667 177 97
12
PasI CC'CwG_GG
Cuts at: 1348 3745 3781 1348
Size: 2397 36 5140
Fragments arranged by size:
5140 2397 36
PciI A'CATG_T
Cuts at: 4083 6472 4083
Size: 2389 5184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 3316 4537 1108
Size: 1659 549 1221 4144
Fragments arranged by size:
4144 1659 1221 549
PfoI T'CCnGG_A
Cuts at: 2625 3273 3621 4167 4281 2625
Size: 648 348 546 114 5917
Fragments arranged by size:
5917 648 546 348 114
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PmlI CAC'GTG
Cuts at: 3184 3184
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5642 5674 7155 7187 3761
Size: 32 1849 32 1481 32 4147
Fragments arranged by size:
4147 1849 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2350 2599 2350
Size: 249 7324
PshAI GACnn'nnGTC
Cuts at: 1613 2296 1613
Size: 683 6890
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PstI C_TGCA'G
Cuts at: 830 2066 2245 4317 4925 830
Size: 1236 179 2072 608 3478
Fragments arranged by size:
3478 2072 1236 608 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 3518 3681 4312 3518
Size: 163 631 6779
Fragments arranged by size:
6779 631 163
SacII CC_GC'GG
Cuts at: 4649 4747 4649
Size: 98 7475
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SanDI GG'GwC_CC
Cuts at: 2599 2599
Size: 7573
SapI GCTCTTCn'nnn_
Cuts at: 3656 4109 3656
Size: 453 7120
SbfI CC_TGCA'GG
Cuts at: 4925 4925
Size: 7573
ScaI AGT'ACT
Cuts at: 3776 4190 5821 7533 3776
Size: 414 1631 1712 3816
Fragments arranged by size:
3816 1712 1631 414
SfcI C'TryA_G
Cuts at: 826 1045 2062 2241 2451 4313 4921 6056 6737
Size: 219 1017 179 210 1862 608 1135 681
Cuts at: 6737 6928 826
Size: 191 1471
Fragments arranged by size:
1862 1471 1135 1017 681 608 219 210
191 179
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 7573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 4590 1700
Size: 2890 4683
SmaI CCC'GGG
Cuts at: 1872 2597 4900 1872
Size: 725 2303 4545
Fragments arranged by size:
4545 2303 725
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2077 2615 3359 5677 6578 6840
Size: 197 926 134 538 744 2318 901 262
Cuts at: 6840 820
Size: 1553
Fragments arranged by size:
2318 1553 926 901 744 538 262 197
134
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 2584 4264 4931 1132
Size: 1452 1680 667 3774
Fragments arranged by size:
3774 1680 1452 667
SspI AAT'ATT
Cuts at: 5 52 4417 5497 5
Size: 47 4365 1080 2081
Fragments arranged by size:
4365 2081 1080 47
StuI AGG'CCT
Cuts at: 1517 3504 4401 1517
Size: 1987 897 4689
Fragments arranged by size:
4689 1987 897
StyI C'CwwG_G
Cuts at: 513 1065 2106 2190 2268 2559 4242 4374 5012
Size: 552 1041 84 78 291 1683 132 638
Cuts at: 5012 513
Size: 3074
Fragments arranged by size:
3074 1683 1041 638 552 291 132 84
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3750 4559 5600 5617 5770 5955 6400
Size: 1255 2170 809 1041 17 153 185 445
Cuts at: 6400 325
Size: 1498
Fragments arranged by size:
2170 1498 1255 1041 809 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3774 4188 5819
Size: 274 80 33 51 1443 1797 414 1631
Cuts at: 5819 7531 96
Size: 1712 138
Fragments arranged by size:
1797 1712 1631 1443 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4007 4039 4416 4448 7533 7565 4007
Size: 32 377 32 3085 32 4015
Fragments arranged by size:
4015 3085 377 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 3967 2197
Size: 1770 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 2595 4898 1870
Size: 725 2303 4545
Fragments arranged by size:
4545 2303 725
XmnI GAAnn'nnTTC
Cuts at: 2471 5702 2471
Size: 3231 4342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI
SfiI SrfI SwaI