Restriction map for
pFN21AB3279
(Circular) (Six-base) MAPSORT of: pFN21AB3279.seq Check: 5729 from: 1 to: 7555
pFN21AB3279 7555 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3145 3649 3145
Size: 504 7051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5365 278
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2744 4898 1937
Size: 397 410 2154 4594
Fragments arranged by size:
4594 2154 410 397
Acc65I G'GTAC_C
Cuts at: 4876 4876
Size: 7555
AclI AA'CG_TT
Cuts at: 1563 2766 5682 6055 1563
Size: 1203 2916 373 3063
Fragments arranged by size:
3063 2916 1203 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4303 5618 7002 7270 1632
Size: 2671 1315 1384 268 1917
Fragments arranged by size:
2671 1917 1384 1315 268
AfeI AGC'GCT
Cuts at: 3509 3978 3509
Size: 469 7086
AflII C'TTAA_G
Cuts at: 820 1017 2896 820
Size: 197 1879 5479
Fragments arranged by size:
5479 1879 197
AflIII A'CryG_T
Cuts at: 1203 3525 3816 3905 4095 6454 7431 1203
Size: 2322 291 89 190 2359 977 1327
Fragments arranged by size:
2359 2322 1327 977 291 190 89
AgeI A'CCGG_T
Cuts at: 6386 6386
Size: 7555
AhdI GACnn_n'nnGTC
Cuts at: 6284 6284
Size: 7555
AleI CACnn'nnGTG
Cuts at: 1166 2135 1166
Size: 969 6586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7555
AlwNI CAG_nnn'CTG
Cuts at: 2158 5298 6870 2158
Size: 3140 1572 2843
Fragments arranged by size:
3140 2843 1572
ApaI G_GGCC'C
Cuts at: 4070 4070
Size: 7555
ApaLI G'TGCA_C
Cuts at: 3533 4463 5612 6768 3533
Size: 930 1149 1156 4320
Fragments arranged by size:
4320 1156 1149 930
ApoI r'AATT_y
Cuts at: 1493 2263 4664 4864 5090 5242 7351 1493
Size: 770 2401 200 226 152 2109 1697
Fragments arranged by size:
2401 2109 1697 770 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 6109 160
Size: 872 5077 1606
Fragments arranged by size:
5077 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 3805 4070 4324 4880 1870
Size: 73 720 1142 265 254 556 4545
Fragments arranged by size:
4545 1142 720 556 265 254 73
BamHI G'GATC_C
Cuts at: 4885 5252 4885
Size: 367 7188
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2182 2675 3761 4027
Size: 325 217 627 342 53 493 1086 266
Cuts at: 4027 4058 4876 6331 618
Size: 31 818 1455 1842
Fragments arranged by size:
1842 1455 1086 818 627 493 342 325
266 217 53 31
BanII G_rGCy'C
Cuts at: 1391 2525 3241 3299 4070 4874 1391
Size: 1134 716 58 771 804 4072
Fragments arranged by size:
4072 1134 804 771 716 58
BbeI G_GCGC'C
Cuts at: 3765 4031 3765
Size: 266 7289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4307 928
Size: 971 2408 4176
Fragments arranged by size:
4176 2408 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5746 5780 1903
Size: 34 3809 34 3678
Fragments arranged by size:
3809 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4540 5447 6657 4540
Size: 907 1210 5438
Fragments arranged by size:
5438 1210 907
BclI T'GATC_A
Cuts at: 1548 4104 4510 1548
Size: 2556 406 4593
Fragments arranged by size:
4593 2556 406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6166 136
Size: 107 122 71 631 605 4494 1525
Fragments arranged by size:
4494 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7550 7550
Size: 7555
BlpI GC'TnA_GC
Cuts at: 4972 4972
Size: 7555
Bme1580I G_kGCm'C
Cuts at: 1790 3537 3722 3913 4070 4467 5616 6772 1790
Size: 1747 185 191 157 397 1149 1156 2573
Fragments arranged by size:
2573 1747 1156 1149 397 191 185 157
BmgBI CAC'GTC
Cuts at: 1318 2365 2548 3181 3346 1318
Size: 1047 183 633 165 5527
Fragments arranged by size:
5527 1047 633 183 165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2717 2954 3107
Size: 416 53 83 399 24 1300 237 153
Cuts at: 3107 4401 5286 6244 442
Size: 1294 885 958 1753
Fragments arranged by size:
1753 1300 1294 958 885 416 399 237
153 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2249 2342 2379 2833 2847 3608 4557
Size: 947 63 93 37 454 14 761 949
Cuts at: 4557 6215 1239
Size: 1658 2579
Fragments arranged by size:
2579 1658 949 947 761 454 93 63
37 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1999 5680 6545 6843 1999
Size: 3681 865 298 2711
Fragments arranged by size:
3681 2711 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 6218 882
Size: 617 3261 1458 2219
Fragments arranged by size:
3261 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 3526 5346 493
Size: 711 2322 1820 2702
Fragments arranged by size:
2702 2322 1820 711
BsaBI GATnn'nnATC
Cuts at: 7549 7549
Size: 7555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3173 3762 3798 4028
Size: 53 83 186 936 1640 589 36 230
Cuts at: 4028 4049 5362 5744 6379 275
Size: 21 1313 382 635 1451
Fragments arranged by size:
1640 1451 1313 936 635 589 382 230
186 83 53 36 21
BsaWI w'CCGG_w
Cuts at: 2620 4640 5988 6386 6660 6807 7218 2620
Size: 2020 1348 398 274 147 411 2957
Fragments arranged by size:
2957 2020 1348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2831 2861 3482 3512 4418
Size: 30 165 30 1428 30 621 30 906
Cuts at: 4418 4448 1178
Size: 30 4285
Fragments arranged by size:
4285 1428 906 621 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 2297 3596 4279 4487 4778 1186
Size: 851 260 1299 683 208 291 3963
Fragments arranged by size:
3963 1299 851 683 291 260 208
BseYI C'CCAG_C
Cuts at: 2404 6758 7254 2404
Size: 4354 496 2705
Fragments arranged by size:
4354 2705 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 1163
Size: 573 593 6389
Fragments arranged by size:
6389 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2278 3755 5005 5766 6794 1540
Size: 275 186 277 1477 1250 761 1028 2301
Fragments arranged by size:
2301 1477 1250 1028 761 277 275 186
BsiHKAI G_wGCw'C
Cuts at: 2950 3241 3537 4175 4283 4467 5616 5701 6772
Size: 291 296 638 108 184 1149 85 1071
Cuts at: 6772 2950
Size: 3733
Fragments arranged by size:
3733 1149 1071 638 296 291 184 108
85
BsmI GAATG_Cn'
Cuts at: 4482 5066 5159 4482
Size: 584 93 6878
Fragments arranged by size:
6878 584 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2525 2950 3241 3299 3537 3722 3913
Size: 399 735 425 291 58 238 185 191
Cuts at: 3913 4070 4175 4283 4467 4874 5616 5701 6772
Size: 157 105 108 184 407 742 85 1071
Cuts at: 6772 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 735 425 407 399 291
238 191 185 184 157 108 105 85
58
BspEI T'CCGG_A
Cuts at: 2620 7218 2620
Size: 4598 2957
BspHI T'CATG_A
Cuts at: 5444 7174 5444
Size: 1730 5825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2557 3145 3170 3649 4910 844
Size: 1494 219 588 25 479 1261 3489
Fragments arranged by size:
3489 1494 1261 588 479 219 25
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 3493 3546 4020 5442 1394
Size: 429 265 1405 53 474 1422 3507
Fragments arranged by size:
3507 1422 1405 474 429 265 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 2076 6050 6224 65
Size: 1183 828 3974 174 1396
Fragments arranged by size:
3974 1396 1183 828 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3691 3764 6199 6386 1700
Size: 110 1881 73 2435 187 2869
Fragments arranged by size:
2869 2435 1881 187 110 73
BsrGI T'GTAC_A
Cuts at: 96 3005 3882 4155 96
Size: 2909 877 273 3496
Fragments arranged by size:
3496 2909 877 273
BssHII G'CGCG_C
Cuts at: 1925 3612 3614 3787 4200 1925
Size: 1687 2 173 413 5280
Fragments arranged by size:
5280 1687 413 173 2
BssSI C'ACGA_G
Cuts at: 3578 5615 6627 3578
Size: 2037 1012 4506
Fragments arranged by size:
4506 2037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7555
BstBI TT'CG_AA
Cuts at: 6359 6359
Size: 7555
BstEII G'GTnAC_C
Cuts at: 1184 3157 3277 1184
Size: 1973 120 5462
Fragments arranged by size:
5462 1973 120
BstXI CCAn_nnnn'nTGG
Cuts at: 4000 4000
Size: 7555
BstYI r'GATC_y
Cuts at: 1972 2026 2955 4346 4679 4885 5252 5637 5654
Size: 54 929 1391 333 206 367 385 17
Cuts at: 5654 7106 7192 7204 7550 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1391 929 385 367 346 333
206 86 54 17 12
BstZ17I GTA'TAC
Cuts at: 2745 2745
Size: 7555
BtgI C'CryG_G
Cuts at: 513 1065 2215 3315 3964 3987 4062 513
Size: 552 1150 1100 649 23 75 4006
Fragments arranged by size:
4006 1150 1100 649 552 75 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 2413 4093 4193 487
Size: 683 56 905 282 1680 100 3849
Fragments arranged by size:
3849 1680 905 683 282 100 56
BtsI GCAGTG_nn'
Cuts at: 3738 3798 4523 5071 5865 5885 3738
Size: 60 725 548 794 20 5408
Fragments arranged by size:
5408 794 725 548 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7555
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2058 2093 529
Size: 35 1494 35 5991
Fragments arranged by size:
5991 1494 35 35
DraI TTT'AAA
Cuts at: 4612 4859 4612
Size: 247 7308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6562 809
Size: 507 5246 1802
Fragments arranged by size:
5246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2643 3318
Size: 54 1425 50 275 18 259 554 675
Cuts at: 3318 3339 3849 3984 3990 4021 4520 5002 5891
Size: 21 510 135 6 31 499 482 889
Cuts at: 5891 7520 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 675 554 510 499 482
275 259 135 54 50 43 31 21
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 5002 1537
Size: 275 3190 4090
Fragments arranged by size:
4090 3190 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5485 1353
Size: 4132 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 2311 3250 6142 6516 6662
Size: 1064 138 600 122 939 2892 374 146
Cuts at: 6662 387
Size: 1280
Fragments arranged by size:
2892 1280 1064 939 600 374 146 138
122
EcoICRI GAG'CTC
Cuts at: 3239 3239
Size: 7555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2249 2342 2379 2833 2847 3608
Size: 393 554 63 93 37 454 14 761
Cuts at: 3608 4303 4557 5618 6215 7002 7270 1239
Size: 695 254 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 761 695 597 554 454
393 268 254 93 63 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 4472 2228
Size: 2244 5311
EcoO109I rG'GnC_Cy
Cuts at: 2155 2221 4067 4505 2155
Size: 66 1846 438 5205
Fragments arranged by size:
5205 1846 438 66
EcoRI G'AATT_C
Cuts at: 2263 4864 5242 2263
Size: 2601 378 4576
Fragments arranged by size:
4576 2601 378
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 7555
FspI TGC'GCA
Cuts at: 6061 6061
Size: 7555
HaeII r_GCGC'y
Cuts at: 1678 3511 3698 3765 3943 3980 4031 6702 1678
Size: 1833 187 67 178 37 51 2671 2531
Fragments arranged by size:
2671 2531 1833 187 178 67 51 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2858 2890 3242
Size: 32 135 32 887 32 367 32 352
Cuts at: 3242 3274 3281 3313 3788 3820 6196 6228 6270
Size: 32 7 32 475 32 2376 32 42
Cuts at: 6270 6302 1373
Size: 32 2626
Fragments arranged by size:
2626 2376 887 475 367 352 135 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 4093 4591 4899 5145 669
Size: 988 281 1116 1039 498 308 246 3079
Fragments arranged by size:
3079 1116 1039 988 498 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7555
HpaI GTT'AAC
Cuts at: 5145 5145
Size: 7555
KasI G'GCGC_C
Cuts at: 3761 4027 3761
Size: 266 7289
KpnI G_GTAC'C
Cuts at: 4880 4880
Size: 7555
MfeI C'AATT_G
Cuts at: 5154 5154
Size: 7555
MluI A'CGCG_T
Cuts at: 3816 3905 7431 3816
Size: 89 3526 3940
Fragments arranged by size:
3940 3526 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 3552 6669 6853 1305
Size: 876 1371 3117 184 2007
Fragments arranged by size:
3117 2007 1371 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4522 10
Size: 54 1425 343 2690 3043
Fragments arranged by size:
3043 2690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2796 5513 5872 6031 518
Size: 648 969 661 2717 359 159 2042
Fragments arranged by size:
2717 2042 969 661 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2217 2344 4602 4971 5648 6796 7041 1606
Size: 611 127 2258 369 677 1148 245 2120
Fragments arranged by size:
2258 2120 1148 677 611 369 245 127
NaeI GCC'GGC
Cuts at: 3693 3766 3693
Size: 73 7482
NarI GG'CG_CC
Cuts at: 3762 4028 3762
Size: 266 7289
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7003
NdeI CA'TA_TG
Cuts at: 387 2925 387
Size: 2538 5017
NgoMIV G'CCGG_C
Cuts at: 3691 3764 3691
Size: 73 7482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7555
NotI GC'GGCC_GC
Cuts at: 5002 5002
Size: 7555
NspI r_CATG'y
Cuts at: 1132 1229 4453 4913 6458 1132
Size: 97 3224 460 1545 2229
Fragments arranged by size:
3224 2229 1545 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7555
PciI A'CATG_T
Cuts at: 6454 6454
Size: 7555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7555
PfoI T'CCnGG_A
Cuts at: 2109 2541 4498 2109
Size: 432 1957 5166
Fragments arranged by size:
5166 1957 432
PmeI GTTT'AAAC
Cuts at: 4859 4859
Size: 7555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 7137 7169 5624
Size: 32 1481 32 6010
Fragments arranged by size:
6010 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2221 2155
Size: 66 7489
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7555
PsiI TTA'TAA
Cuts at: 5125 5125
Size: 7555
PspOMI G'GGCC_C
Cuts at: 4066 4066
Size: 7555
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2728 2760 3374 3406 2728
Size: 32 614 32 6877
Fragments arranged by size:
6877 614 32 32
PstI C_TGCA'G
Cuts at: 830 2244 2349 3567 3660 3840 4907 830
Size: 1414 105 1218 93 180 1067 3478
Fragments arranged by size:
3478 1414 1218 1067 180 105 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7555
PvuII CAG'CTG
Cuts at: 4602 4602
Size: 7555
SacI G_AGCT'C
Cuts at: 3241 3241
Size: 7555
SacII CC_GC'GG
Cuts at: 2218 2218
Size: 7555
SalI G'TCGA_C
Cuts at: 1936 4897 1936
Size: 2961 4594
SbfI CC_TGCA'GG
Cuts at: 4907 4907
Size: 7555
ScaI AGT'ACT
Cuts at: 5803 7515 5803
Size: 1712 5843
SexAI A'CCwGG_T
Cuts at: 4851 4851
Size: 7555
SfcI C'TryA_G
Cuts at: 826 1045 2240 2345 3563 3596 3656 3836 4350
Size: 219 1195 105 1218 33 60 180 514
Cuts at: 4350 4903 6038 6719 6910 826
Size: 553 1135 681 191 1471
Fragments arranged by size:
1471 1218 1195 1135 681 553 514 219
191 180 105 60 33
SfoI GGC'GCC
Cuts at: 3763 4029 3763
Size: 266 7289
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
SgrAI Cr'CCGG_yG
Cuts at: 1700 3691 3764 1700
Size: 1991 73 5491
Fragments arranged by size:
5491 1991 73
SmaI CCC'GGG
Cuts at: 1872 4326 4882 1872
Size: 2454 556 4545
Fragments arranged by size:
4545 2454 556
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2014 2896 5659 6560 6822 820
Size: 197 926 71 882 2763 901 262 1553
Fragments arranged by size:
2763 1553 926 901 882 262 197 71
SnaBI TAC'GTA
Cuts at: 493 5346 493
Size: 4853 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7555
SphI G_CATG'C
Cuts at: 1132 4453 4913 1132
Size: 3321 460 3774
Fragments arranged by size:
3774 3321 460
SrfI GCCC'GGGC
Cuts at: 4326 4326
Size: 7555
SspI AAT'ATT
Cuts at: 5 52 5479 5
Size: 47 5427 2081
Fragments arranged by size:
5427 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7555
StyI C'CwwG_G
Cuts at: 513 1065 4994 513
Size: 552 3929 3074
Fragments arranged by size:
3929 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 4046 4319 5582 5599 5752 5937
Size: 1255 485 1981 273 1263 17 153 185
Cuts at: 5937 6382 325
Size: 445 1498
Fragments arranged by size:
1981 1498 1263 1255 485 445 273 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3005 3381 3882
Size: 274 80 33 51 1443 1028 376 501
Cuts at: 3882 4155 5801 7513 96
Size: 273 1646 1712 138
Fragments arranged by size:
1712 1646 1443 1028 501 376 274 273
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 2940 2972 7515 7547 2769
Size: 32 139 32 4543 32 2777
Fragments arranged by size:
4543 2777 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7555
XbaI T'CTAG_A
Cuts at: 4891 4891
Size: 7555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2884 3328 2884
Size: 444 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7555
XmaI C'CCGG_G
Cuts at: 1870 4324 4880 1870
Size: 2454 556 4545
Fragments arranged by size:
4545 2454 556
XmnI GAAnn'nnTTC
Cuts at: 2423 5684 2423
Size: 3261 4294
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5363 276
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI NruI
NsiI PacI PmlI PspXI RsrII SanDI SapI SfiI
SwaI