Restriction map for
pFN21AB3277
(Circular) (Six-base) MAPSORT of: pFN21AB3277.seq Check: 6484 from: 1 to: 6520
pFN21AB3277 6520 bp
With 182 enzymes: SgfI *
September 25, 2018 16:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 2671 3841 2671
Size: 1170 5350
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 3408 4583 5967 6235 1632
Size: 1100 676 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 1100 676 268
AfeI AGC'GCT
Cuts at: 2748 2748
Size: 6520
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 5419 6396 1203
Size: 4216 977 1327
Fragments arranged by size:
4216 1327 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 2690 5249 2690
Size: 2559 3961
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 2246 4263 5835 2246
Size: 2017 1572 2931
Fragments arranged by size:
2931 2017 1572
ApaI G_GGCC'C
Cuts at: 2705 2705
Size: 6520
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2297 2420 2446 3829 4055 4207 6316 1493
Size: 804 123 26 1383 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 804 226 152 123 26
AseI AT'TA_AT
Cuts at: 160 1032 2973 5074 160
Size: 872 1941 2101 1606
Fragments arranged by size:
2101 1941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 3069 3666 3845 1870
Size: 73 1126 597 179 4545
Fragments arranged by size:
4545 1126 597 179 73
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 3657 3841 5296 618
Size: 325 217 627 884 986 184 1455 1842
Fragments arranged by size:
1842 1455 986 884 627 325 217 184
BanII G_rGCy'C
Cuts at: 1391 2705 3839 1391
Size: 1314 1134 4072
Fragments arranged by size:
4072 1314 1134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2648 928
Size: 971 749 4800
Fragments arranged by size:
4800 971 749
BbvCI CC'TCA_GC
Cuts at: 3442 3442
Size: 6520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4711 4745 1903
Size: 34 50 34 2690 34 3678
Fragments arranged by size:
3678 2690 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2959 4412 5622 2959
Size: 1453 1210 3857
Fragments arranged by size:
3857 1453 1210
BclI T'GATC_A
Cuts at: 1548 3340 1548
Size: 1792 4728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5131 136
Size: 107 122 71 631 605 3459 1525
Fragments arranged by size:
3459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 2890 3937 2890
Size: 1047 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2705 4581 5737 1790
Size: 915 1876 1156 2573
Fragments arranged by size:
2573 1876 1156 915
BmgBI CAC'GTC
Cuts at: 1318 3673 1318
Size: 2355 4165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 2577 2841
Size: 416 53 83 399 24 943 217 264
Cuts at: 2841 4251 5209 442
Size: 1410 958 1753
Fragments arranged by size:
1753 1410 958 943 416 399 264 217
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2413 3123 3230 3472 5180 1239
Size: 884 290 710 107 242 1708 2579
Fragments arranged by size:
2579 1708 884 710 290 242 107
Bpu10I CC'TnA_GC
Cuts at: 3266 3442 3266
Size: 176 6344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3066 3197 3318 4645 5510 5808 3066
Size: 131 121 1327 865 298 3778
Fragments arranged by size:
3778 1327 865 298 131 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3611 5183 882
Size: 617 2112 1572 2219
Fragments arranged by size:
2219 2112 1572 617
BsaAI yAC'GTr
Cuts at: 493 1204 3541 3574 4311 493
Size: 711 2337 33 737 2702
Fragments arranged by size:
2702 2337 737 711 33
BsaBI GATnn'nnATC
Cuts at: 2789 6514 2789
Size: 3725 2795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4327 4709 5344 275
Size: 53 83 186 936 2794 382 635 1451
Fragments arranged by size:
2794 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4953 5351 5625 5772 6183 4953
Size: 398 274 147 411 5290
Fragments arranged by size:
5290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3220 3250 1178
Size: 30 165 30 1152 30 635 30 4448
Fragments arranged by size:
4448 1152 635 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2646 3430 1186
Size: 934 526 784 4276
Fragments arranged by size:
4276 934 784 526
BseYI C'CCAG_C
Cuts at: 3239 3423 3785 5723 6219 3239
Size: 184 362 1938 496 3540
Fragments arranged by size:
3540 1938 496 362 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5947
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2652 3195 4581 4666 5737 2652
Size: 543 1386 85 1071 3435
Fragments arranged by size:
3435 1386 1071 543 85
BsmI GAATG_Cn'
Cuts at: 2631 4031 4124 2631
Size: 1400 93 5027
Fragments arranged by size:
5027 1400 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3606 3606
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2652 2705 3195 3839 4581 4666 5737
Size: 399 862 53 490 644 742 85 1071
Cuts at: 5737 1391
Size: 2174
Fragments arranged by size:
2174 1071 862 742 644 490 399 85
53
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3875 844
Size: 3031 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 2571 3362 3432 4407 1394
Size: 429 317 431 791 70 975 3507
Fragments arranged by size:
3507 975 791 431 429 317 70
BsrDI GCAATG_nn'
Cuts at: 65 1248 2939 5015 5189 65
Size: 1183 1691 2076 174 1396
Fragments arranged by size:
2076 1691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 5164 5351 1700
Size: 110 221 3133 187 2869
Fragments arranged by size:
3133 2869 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 2265 5324 2265
Size: 3059 3461
BstEII G'GTnAC_C
Cuts at: 1184 2800 1184
Size: 1616 4904
BstXI CCAn_nnnn'nTGG
Cuts at: 3467 3467
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2870 3850 4217 4602 4619 6071 6157 6169
Size: 898 980 367 385 17 1452 86 12
Cuts at: 6169 6515 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 980 898 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3042 3042
Size: 6520
BtgI C'CryG_G
Cuts at: 513 1065 3622 513
Size: 552 2557 3411
Fragments arranged by size:
3411 2557 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2012 3446 487
Size: 683 56 786 1434 3561
Fragments arranged by size:
3561 1434 786 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2127 3472 3637 4036 4830 4850 2015
Size: 112 1345 165 399 794 20 3685
Fragments arranged by size:
3685 1345 794 399 165 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 2763 3824 2763
Size: 1061 5459
DraIII CAC_nnn'GTG
Cuts at: 3596 3596
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 3967 4856
Size: 54 1425 50 275 18 423 1714 889
Cuts at: 4856 6485 8
Size: 1629 43
Fragments arranged by size:
1714 1629 1425 889 423 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3439 4450 1353
Size: 2086 1011 3423
Fragments arranged by size:
3423 2086 1011
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2797 3803 5107 5481 5627 387
Size: 1064 138 1208 1006 1304 374 146 1280
Fragments arranged by size:
1304 1280 1208 1064 1006 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2413 2732 3123 3230 3408 3472
Size: 393 491 290 319 391 107 178 64
Cuts at: 3472 4583 5180 5967 6235 1239
Size: 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 491 393 391 319
290 268 178 107 64
EcoO109I rG'GnC_Cy
Cuts at: 2702 2702
Size: 6520
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
EcoRV GAT'ATC
Cuts at: 2787 3765 2787
Size: 978 5542
FspI TGC'GCA
Cuts at: 2126 5026 2126
Size: 2900 3620
HaeII r_GCGC'y
Cuts at: 1678 2750 5667 1678
Size: 1072 2917 2531
Fragments arranged by size:
2917 2531 1072
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2676 2708 3022 3054 3337
Size: 32 135 32 1104 32 314 32 283
Cuts at: 3337 3369 5161 5193 5235 5267 1373
Size: 32 1792 32 42 32 2626
Fragments arranged by size:
2626 1792 1104 314 283 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3864 4110 669
Size: 988 281 1926 246 3079
Fragments arranged by size:
3079 1926 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2887 748
Size: 2139 4381
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 2675 3845 2675
Size: 1170 5350
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2898 5634 5818 1305
Size: 834 759 2736 184 2007
Fragments arranged by size:
2736 2007 834 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 4275
Fragments arranged by size:
4275 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 2732 3465 4478 4837 4996 518
Size: 648 1063 503 733 1013 359 159 2042
Fragments arranged by size:
2042 1063 1013 733 648 503 359 159
MspA1I CmG'CkG
Cuts at: 1606 2807 3116 3936 4613 5761 6006 1606
Size: 1201 309 820 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 820 677 309 245
NaeI GCC'GGC
Cuts at: 2033 2033
Size: 6520
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5968
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 2031 2031
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2288 3878 5423 1132
Size: 97 1059 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 1059 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6520
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2246 1108
Size: 1138 5382
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PmlI CAC'GTG
Cuts at: 3574 3574
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2204 1613
Size: 591 5929
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2701 2701
Size: 6520
PstI C_TGCA'G
Cuts at: 830 2394 3872 830
Size: 1564 1478 3478
Fragments arranged by size:
3478 1564 1478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 3116 3116
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 3439 3439
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2212 2390 2511 3868 5003 5684 5875
Size: 219 1167 178 121 1357 1135 681 191
Cuts at: 5875 826
Size: 1471
Fragments arranged by size:
1471 1357 1167 1135 681 219 191 178
121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 2031 1700
Size: 331 6189
SmaI CCC'GGG
Cuts at: 1872 3668 3847 1872
Size: 1796 179 4545
Fragments arranged by size:
4545 1796 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3069 3081 3176 3333 4624 5525
Size: 197 926 1126 12 95 157 1291 901
Cuts at: 5525 5787 820
Size: 262 1553
Fragments arranged by size:
1553 1291 1126 926 901 262 197 157
95 12
SnaBI TAC'GTA
Cuts at: 493 3541 4311 493
Size: 3048 770 2702
Fragments arranged by size:
3048 2702 770
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 3215 1517
Size: 1698 4822
StyI C'CwwG_G
Cuts at: 513 1065 3003 3729 3959 513
Size: 552 1938 726 230 3074
Fragments arranged by size:
3074 1938 726 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 3245 4547 4564 4717 4902 5347
Size: 1255 873 792 1302 17 153 185 445
Cuts at: 5347 325
Size: 1498
Fragments arranged by size:
1498 1302 1255 873 792 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4766 6478 96
Size: 274 80 33 51 1443 2789 1712 138
Fragments arranged by size:
2789 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 3220 3252 3419 3451 3635 3667 6480
Size: 32 1126 32 167 32 184 32 2813
Cuts at: 6480 6512 2062
Size: 32 2070
Fragments arranged by size:
2813 2070 1126 184 167 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2686
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 3069 1943
Size: 1126 5394
XmaI C'CCGG_G
Cuts at: 1870 3666 3845 1870
Size: 1796 179 4545
Fragments arranged by size:
4545 1796 179
XmnI GAAnn'nnTTC
Cuts at: 4649 4649
Size: 6520
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI