Restriction map for
pFN21AB3272
(Circular) (Six-base) MAPSORT of: pFN21AB3272.seq Check: 545 from: 1 to: 8500
pFN21AB3272 8500 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 3876 2146
Size: 1730 6770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3159 4269 6310 278
Size: 53 83 186 936 1623 1110 2041 2468
Fragments arranged by size:
2468 2041 1623 1110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4809 4920 4959 5843 1937
Size: 2872 111 39 884 4594
Fragments arranged by size:
4594 2872 884 111 39
Acc65I G'GTAC_C
Cuts at: 5821 5821
Size: 8500
AclI AA'CG_TT
Cuts at: 1563 6627 7000 1563
Size: 5064 373 3063
Fragments arranged by size:
5064 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3502 3808 4039 4949 5525 6563 7947 8215
Size: 1870 306 231 910 576 1038 1384 268
Cuts at: 8215 1632
Size: 1917
Fragments arranged by size:
1917 1870 1384 1038 910 576 306 268
231
AfeI AGC'GCT
Cuts at: 4229 4229
Size: 8500
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8303
AflIII A'CryG_T
Cuts at: 1203 2988 3450 7399 8376 1203
Size: 1785 462 3949 977 1327
Fragments arranged by size:
3949 1785 1327 977 462
AgeI A'CCGG_T
Cuts at: 7331 7331
Size: 8500
AhdI GACnn_n'nnGTC
Cuts at: 7229 7229
Size: 8500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 8468
AlwNI CAG_nnn'CTG
Cuts at: 2788 2907 6243 7815 2788
Size: 119 3336 1572 3473
Fragments arranged by size:
3473 3336 1572 119
ApaI G_GGCC'C
Cuts at: 3096 3383 3096
Size: 287 8213
ApaLI G'TGCA_C
Cuts at: 6557 7713 6557
Size: 1156 7344
ApoI r'AATT_y
Cuts at: 1493 2663 5373 5809 6035 6187 8296 1493
Size: 1170 2710 436 226 152 2109 1697
Fragments arranged by size:
2710 2109 1697 1170 436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7054 160
Size: 872 6022 1606
Fragments arranged by size:
6022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 3193 3384 3996 4214 4412 5162
Size: 73 174 1076 191 612 218 198 750
Cuts at: 5162 5825 1870
Size: 663 4545
Fragments arranged by size:
4545 1076 750 663 612 218 198 191
174 73
BamHI G'GATC_C
Cuts at: 3765 4285 4446 5830 6197 3765
Size: 520 161 1384 367 6068
Fragments arranged by size:
6068 1384 520 367 161
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3680 4382 5821 7276 618
Size: 325 217 627 1893 702 1439 1455 1842
Fragments arranged by size:
1893 1842 1455 1439 702 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2595 2782 3096 3383 4152 4221 4413
Size: 677 527 187 314 287 769 69 192
Cuts at: 4413 4638 4692 4794 5347 5819 1391
Size: 225 54 102 553 472 4072
Fragments arranged by size:
4072 769 677 553 527 472 314 287
225 192 187 102 69 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 2510 4533 4615 928
Size: 971 387 224 2023 82 4813
Fragments arranged by size:
4813 2023 971 387 224 82
BbvCI CC'TCA_GC
Cuts at: 2321 5330 5612 2321
Size: 3009 282 5209
Fragments arranged by size:
5209 3009 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2296 2330 4025 4059 6691 6725 1903
Size: 34 359 34 1695 34 2632 34 3678
Fragments arranged by size:
3678 2632 1695 359 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6392 7602 6392
Size: 1210 7290
BclI T'GATC_A
Cuts at: 1548 2139 2520 1548
Size: 591 381 7528
Fragments arranged by size:
7528 591 381
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3387 7111 136
Size: 107 122 71 631 605 1715 3724 1525
Fragments arranged by size:
3724 1715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5268 8495 5268
Size: 3227 5273
BlpI GC'TnA_GC
Cuts at: 2330 2594 2847 4367 5917 2330
Size: 264 253 1520 1550 4913
Fragments arranged by size:
4913 1550 1520 264 253
Bme1580I G_kGCm'C
Cuts at: 1790 3096 3383 3558 3912 4387 6561 7717 1790
Size: 1306 287 175 354 475 2174 1156 2573
Fragments arranged by size:
2573 2174 1306 1156 475 354 287 175
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3197 4284 4771
Size: 416 53 83 399 24 1780 1087 487
Cuts at: 4771 6231 7189 442
Size: 1460 958 1753
Fragments arranged by size:
1780 1753 1460 1087 958 487 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4501 4501
Size: 8500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2185 4244 4652 5594 5714 7160 1239
Size: 851 95 2059 408 942 120 1446 2579
Fragments arranged by size:
2579 2059 1446 942 851 408 120 95
Bpu10I CC'TnA_GC
Cuts at: 2321 2781 3956 5330 5612 2321
Size: 460 1175 1374 282 5209
Fragments arranged by size:
5209 1374 1175 460 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6625 7490 7788 6625
Size: 865 298 7337
Fragments arranged by size:
7337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3698 4548 7163 882
Size: 617 2199 850 2615 2219
Fragments arranged by size:
2615 2219 2199 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 2880 2991 6291 493
Size: 711 1676 111 3300 2702
Fragments arranged by size:
3300 2702 1676 711 111
BsaBI GATnn'nnATC
Cuts at: 3359 8494 3359
Size: 5135 3365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 4266 6307 6689
Size: 53 83 186 936 1623 1110 2041 382
Cuts at: 6689 7324 275
Size: 635 1451
Fragments arranged by size:
2041 1623 1451 1110 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4017 4657 6933 7331 7605 7752 8163 4017
Size: 640 2276 398 274 147 411 4354
Fragments arranged by size:
4354 2276 640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2957 2987 3584 3614 4385
Size: 30 165 30 1554 30 597 30 771
Cuts at: 4385 4415 1178
Size: 30 5263
Fragments arranged by size:
5263 1554 771 597 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2965 3236 3560 4517 4700 5587 5629 1186
Size: 1779 271 324 957 183 887 42 4057
Fragments arranged by size:
4057 1779 957 887 324 271 183 42
BseYI C'CCAG_C
Cuts at: 2074 3084 3335 3376 4114 7703 8199 2074
Size: 1010 251 41 738 3589 496 2375
Fragments arranged by size:
3589 2375 1010 738 496 251 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3182 1163
Size: 573 1446 6481
Fragments arranged by size:
6481 1446 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4102 5950 6711 7739 1540
Size: 275 186 2101 1848 761 1028 2301
Fragments arranged by size:
2301 2101 1848 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2581 2595 3228 4152 4221 4692 4794 5215 6561
Size: 14 633 924 69 471 102 421 1346
Cuts at: 6561 6646 7717 2581
Size: 85 1071 3364
Fragments arranged by size:
3364 1346 1071 924 633 471 421 102
85 69 14
BsmI GAATG_Cn'
Cuts at: 2413 4171 6011 6104 2413
Size: 1758 1840 93 4809
Fragments arranged by size:
4809 1840 1758 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2141 3656 3854 4877 2141
Size: 1515 198 1023 5764
Fragments arranged by size:
5764 1515 1023 198
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2581 2595 2782 3096 3228 3383
Size: 399 278 513 14 187 314 132 155
Cuts at: 3383 3558 3912 4152 4221 4387 4413 4638 4692
Size: 175 354 240 69 166 26 225 54
Cuts at: 4692 4794 5215 5347 5819 6561 6646 7717 1391
Size: 102 421 132 472 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 472 421 399 354
314 278 240 225 187 175 166 155
132 132 102 85 69 54 26 14
BspEI T'CCGG_A
Cuts at: 4017 8163 4017
Size: 4146 4354
BspHI T'CATG_A
Cuts at: 6389 8119 6389
Size: 1730 6770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2146 2739 3294 3876 5855 844
Size: 1302 593 555 582 1979 3489
Fragments arranged by size:
3489 1979 1302 593 582 555
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 2806 3771 4872 6387 1394
Size: 429 299 684 965 1101 1515 3507
Fragments arranged by size:
3507 1515 1101 965 684 429 299
BsrDI GCAATG_nn'
Cuts at: 65 1248 3495 5683 6995 7169 65
Size: 1183 2247 2188 1312 174 1396
Fragments arranged by size:
2247 2188 1396 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3106 4101 7144 7331 1700
Size: 110 1296 995 3043 187 2869
Fragments arranged by size:
3043 2869 1296 995 187 110
BsrGI T'GTAC_A
Cuts at: 96 3447 96
Size: 3351 5149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8500
BssSI C'ACGA_G
Cuts at: 2892 3926 6560 7572 2892
Size: 1034 2634 1012 3820
Fragments arranged by size:
3820 2634 1034 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4111 794
Size: 3317 5183
BstBI TT'CG_AA
Cuts at: 7304 7304
Size: 8500
BstEII G'GTnAC_C
Cuts at: 1184 3002 1184
Size: 1818 6682
BstYI r'GATC_y
Cuts at: 1972 2785 2970 3765 4285 4446 5178 5268 5830
Size: 813 185 795 520 161 732 90 562
Cuts at: 5830 6197 6582 6599 8051 8137 8149 8495 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 813 795 732 562 520 385
367 346 185 161 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4537 4596 4537
Size: 59 8441
BtgI C'CryG_G
Cuts at: 513 1065 2013 3118 3303 3777 3938 3961 4378
Size: 552 948 1105 185 474 161 23 417
Cuts at: 4378 513
Size: 4635
Fragments arranged by size:
4635 1105 948 552 474 417 185 161
23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2417 3405 4374 4765 487
Size: 683 56 1191 988 969 391 4222
Fragments arranged by size:
4222 1191 988 969 683 391 56
BtsI GCAGTG_nn'
Cuts at: 2545 3226 3757 6016 6810 6830 2545
Size: 681 531 2259 794 20 4215
Fragments arranged by size:
4215 2259 794 681 531 20
ClaI AT'CG_AT
Cuts at: 1554 2651 1554
Size: 1097 7403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8465
DraI TTT'AAA
Cuts at: 2530 5804 2530
Size: 3274 5226
DraIII CAC_nnn'GTG
Cuts at: 4111 4111
Size: 8500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4805 7507 809
Size: 507 3489 2702 1802
Fragments arranged by size:
3489 2702 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 4099 5564
Size: 54 1425 50 275 18 1425 844 1465
Cuts at: 5564 5947 6836 8465 8
Size: 383 889 1629 43
Fragments arranged by size:
1629 1465 1425 1425 889 844 383 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4099 5947 1537
Size: 275 2287 1848 4090
Fragments arranged by size:
4090 2287 1848 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2621 3614 4990 5577 5765 6430 1353
Size: 689 579 993 1376 587 188 665 3423
Fragments arranged by size:
3423 1376 993 689 665 587 579 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 4114 4994 7087 7461 7607
Size: 1064 138 624 1901 880 2093 374 146
Cuts at: 7607 387
Size: 1280
Fragments arranged by size:
2093 1901 1280 1064 880 624 374 146
138
EcoICRI GAG'CTC
Cuts at: 2593 4150 4219 4690 4792 2593
Size: 1557 69 471 102 6301
Fragments arranged by size:
6301 1557 471 102 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2185 3502 3808 4039 4244 4652
Size: 393 458 95 1317 306 231 205 408
Cuts at: 4652 4949 5525 5594 5714 6563 7160 7947 8215
Size: 297 576 69 120 849 597 787 268
Cuts at: 8215 1239
Size: 1524
Fragments arranged by size:
1524 1317 849 787 597 576 458 408
393 306 297 268 231 205 120 95
69
EcoO109I rG'GnC_Cy
Cuts at: 3380 3789 4005 4119 4945 3380
Size: 409 216 114 826 6935
Fragments arranged by size:
6935 826 409 216 114
EcoRI G'AATT_C
Cuts at: 2663 5809 6187 2663
Size: 3146 378 4976
Fragments arranged by size:
4976 3146 378
EcoRV GAT'ATC
Cuts at: 2203 2203
Size: 8500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 3532 4858 5606 2527
Size: 1005 1326 748 5421
Fragments arranged by size:
5421 1326 1005 748
FspI TGC'GCA
Cuts at: 3053 5675 7006 3053
Size: 2622 1331 4547
Fragments arranged by size:
4547 2622 1331
HaeII r_GCGC'y
Cuts at: 1678 4231 7647 1678
Size: 2553 3416 2531
Fragments arranged by size:
3416 2553 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4238 4270 7141 7173 7215
Size: 32 135 32 2666 32 2871 32 42
Cuts at: 7215 7247 1373
Size: 32 2626
Fragments arranged by size:
2871 2666 2626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 3294 3403 4921 4981 5844
Size: 988 281 427 929 109 1518 60 863
Cuts at: 5844 6090 669
Size: 246 3079
Fragments arranged by size:
3079 1518 988 929 863 427 281 246
109 60
HindIII A'AGCT_T
Cuts at: 748 3033 748
Size: 2285 6215
HpaI GTT'AAC
Cuts at: 2365 6090 2365
Size: 3725 4775
KpnI G_GTAC'C
Cuts at: 5825 5825
Size: 8500
MfeI C'AATT_G
Cuts at: 6099 6099
Size: 8500
MluI A'CGCG_T
Cuts at: 8376 8376
Size: 8500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 5372 7614 7798 1305
Size: 3186 881 2242 184 2007
Fragments arranged by size:
3186 2242 2007 881 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 10
Size: 54 1425 343 1425 5253
Fragments arranged by size:
5253 1425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 3983 5069 6458 6817 6976 518
Size: 648 1420 1397 1086 1389 359 159 2042
Fragments arranged by size:
2042 1420 1397 1389 1086 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2342 3010 3045 3325 3343 3963 5611 5734
Size: 736 668 35 280 18 620 1648 123
Cuts at: 5734 5916 6593 7741 7986 1606
Size: 182 677 1148 245 2120
Fragments arranged by size:
2120 1648 1148 736 677 668 620 280
245 182 123 35 18
NaeI GCC'GGC
Cuts at: 4103 4103
Size: 8500
NcoI C'CATG_G
Cuts at: 513 1065 3118 3777 513
Size: 552 2053 659 5236
Fragments arranged by size:
5236 2053 659 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8500
NgoMIV G'CCGG_C
Cuts at: 4101 4101
Size: 8500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8500
NotI GC'GGCC_GC
Cuts at: 5947 5947
Size: 8500
NspI r_CATG'y
Cuts at: 1132 1229 2938 3454 3982 5068 5858 7403 1132
Size: 97 1709 516 528 1086 790 1545 2229
Fragments arranged by size:
2229 1709 1545 1086 790 528 516 97
PasI CC'CwG_GG
Cuts at: 1348 2228 1348
Size: 880 7620
PciI A'CATG_T
Cuts at: 3450 7399 3450
Size: 3949 4551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3939 1108
Size: 2831 5669
PfoI T'CCnGG_A
Cuts at: 4221 4629 5571 4221
Size: 408 942 7150
Fragments arranged by size:
7150 942 408
PmeI GTTT'AAAC
Cuts at: 5804 5804
Size: 8500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 6569 6601 8082 8114 3584
Size: 32 2953 32 1481 32 3970
Fragments arranged by size:
3970 2953 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3789 4119 3789
Size: 330 8170
PshAI GACnn'nnGTC
Cuts at: 1613 4955 1613
Size: 3342 5158
PsiI TTA'TAA
Cuts at: 6070 6070
Size: 8500
PspOMI G'GGCC_C
Cuts at: 3092 3379 3092
Size: 287 8213
PspXI vC'TCGA_Gb
Cuts at: 3193 3193
Size: 8500
PstI C_TGCA'G
Cuts at: 830 3233 3648 4495 5121 5852 830
Size: 2403 415 847 626 731 3478
Fragments arranged by size:
3478 2403 847 731 626 415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8500
PvuII CAG'CTG
Cuts at: 2342 3010 3325 3343 5611 5734 2342
Size: 668 315 18 2268 123 5108
Fragments arranged by size:
5108 2268 668 315 123 18
SacI G_AGCT'C
Cuts at: 2595 4152 4221 4692 4794 2595
Size: 1557 69 471 102 6301
Fragments arranged by size:
6301 1557 471 102 69
SacII CC_GC'GG
Cuts at: 3964 3964
Size: 8500
SalI G'TCGA_C
Cuts at: 1936 4919 5842 1936
Size: 2983 923 4594
Fragments arranged by size:
4594 2983 923
SanDI GG'GwC_CC
Cuts at: 3789 3789
Size: 8500
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 8500
SbfI CC_TGCA'GG
Cuts at: 5852 5852
Size: 8500
ScaI AGT'ACT
Cuts at: 4457 4760 6748 8460 4457
Size: 303 1988 1712 4497
Fragments arranged by size:
4497 1988 1712 303
SexAI A'CCwGG_T
Cuts at: 4800 4800
Size: 8500
SfcI C'TryA_G
Cuts at: 826 1045 3229 3573 3644 4491 5117 5848 6983
Size: 219 2184 344 71 847 626 731 1135
Cuts at: 6983 7664 7855 826
Size: 681 191 1471
Fragments arranged by size:
2184 1471 1135 847 731 681 626 344
219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8500
SmaI CCC'GGG
Cuts at: 1872 3998 4414 5827 1872
Size: 2126 416 1413 4545
Fragments arranged by size:
4545 2126 1413 416
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3193 6604 7505 7767 820
Size: 197 926 1250 3411 901 262 1553
Fragments arranged by size:
3411 1553 1250 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6291 493
Size: 5798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8500
SphI G_CATG'C
Cuts at: 1132 5858 1132
Size: 4726 3774
SspI AAT'ATT
Cuts at: 5 52 4560 6424 5
Size: 47 4508 1864 2081
Fragments arranged by size:
4508 2081 1864 47
StuI AGG'CCT
Cuts at: 1517 3263 1517
Size: 1746 6754
StyI C'CwwG_G
Cuts at: 513 1065 3118 3258 3777 4404 4740 4833 5939
Size: 552 2053 140 519 627 336 93 1106
Cuts at: 5939 513
Size: 3074
Fragments arranged by size:
3074 2053 1106 627 552 519 336 140
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6527 6544 6697 6882 7327 325
Size: 1255 4947 17 153 185 445 1498
Fragments arranged by size:
4947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3447 4455 4758
Size: 274 80 33 51 1443 1470 1008 303
Cuts at: 4758 4932 5061 6746 8458 96
Size: 174 129 1685 1712 138
Fragments arranged by size:
1712 1685 1470 1443 1008 303 274 174
138 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4385 4417 5028 5060 8460 8492 4385
Size: 32 611 32 3400 32 4393
Fragments arranged by size:
4393 3400 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8500
XbaI T'CTAG_A
Cuts at: 5836 5836
Size: 8500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4678 4678
Size: 8500
XhoI C'TCGA_G
Cuts at: 1943 3193 1943
Size: 1250 7250
XmaI C'CCGG_G
Cuts at: 1870 3996 4412 5825 1870
Size: 2126 416 1413 4545
Fragments arranged by size:
4545 2126 1413 416
XmnI GAAnn'nnTTC
Cuts at: 3599 6629 3599
Size: 3030 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3157 4267 6308 276
Size: 53 83 186 936 1623 1110 2041 2468
Fragments arranged by size:
2468 2041 1623 1110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PsrI RsrII SfiI SfoI SrfI SwaI