Restriction map for
pFN21AB3260
(Circular) (Six-base) MAPSORT of: pFN21AB3260.seq Check: 5614 from: 1 to: 9550
pFN21AB3260 9550 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3097 5786 3097
Size: 2689 6861
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3744 4086 7360 278
Size: 53 83 186 936 2208 342 3274 2468
Fragments arranged by size:
3274 2468 2208 936 342 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 3273 5361 6893 1937
Size: 1228 108 2088 1532 4594
Fragments arranged by size:
4594 2088 1532 1228 108
Acc65I G'GTAC_C
Cuts at: 5241 6871 5241
Size: 1630 7920
AclI AA'CG_TT
Cuts at: 1563 7677 8050 1563
Size: 6114 373 3063
Fragments arranged by size:
6114 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4112 5423 7613 8997 9265 1632
Size: 2480 1311 2190 1384 268 1917
Fragments arranged by size:
2480 2190 1917 1384 1311 268
AfeI AGC'GCT
Cuts at: 6245 6245
Size: 9550
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9353
AflIII A'CryG_T
Cuts at: 1203 2496 3008 6546 6642 8449 9426 1203
Size: 1293 512 3538 96 1807 977 1327
Fragments arranged by size:
3538 1807 1327 1293 977 512 96
AgeI A'CCGG_T
Cuts at: 8381 8381
Size: 9550
AhdI GACnn_n'nnGTC
Cuts at: 8279 8279
Size: 9550
AleI CACnn'nnGTG
Cuts at: 1166 2174 2981 5652 5713 6156 6641 1166
Size: 1008 807 2671 61 443 485 4075
Fragments arranged by size:
4075 2671 1008 807 485 443 61
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3147 1184
Size: 1963 7587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 4238 4270 2669
Size: 32 1537 32 7949
Fragments arranged by size:
7949 1537 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2863 3232 5476 5752 6672 6754 7293 8865 2863
Size: 369 2244 276 920 82 539 1572 3548
Fragments arranged by size:
3548 2244 1572 920 539 369 276 82
ApaI G_GGCC'C
Cuts at: 6171 6267 6171
Size: 96 9454
ApaLI G'TGCA_C
Cuts at: 3716 7607 8763 3716
Size: 3891 1156 4503
Fragments arranged by size:
4503 3891 1156
ApoI r'AATT_y
Cuts at: 1493 6859 7085 7237 9346 1493
Size: 5366 226 152 2109 1697
Fragments arranged by size:
5366 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8104 160
Size: 872 7072 1606
Fragments arranged by size:
7072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9550
AvaI C'yCGr_G
Cuts at: 1870 1943 3387 3509 3983 4163 4291 5828 5855
Size: 73 1444 122 474 180 128 1537 27
Cuts at: 5855 6322 6875 1870
Size: 467 553 4545
Fragments arranged by size:
4545 1537 1444 553 474 467 180 128
122 73 27
BamHI G'GATC_C
Cuts at: 4309 6880 7247 4309
Size: 2571 367 6612
Fragments arranged by size:
6612 2571 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3344 4992 5241 5303 6412
Size: 325 217 627 1557 1648 249 62 1109
Cuts at: 6412 6613 6871 8326 618
Size: 201 258 1455 1842
Fragments arranged by size:
1842 1648 1557 1455 1109 627 325 258
249 217 201 62
BanII G_rGCy'C
Cuts at: 1391 3387 3777 4127 6108 6171 6267 6383 6869
Size: 1996 390 350 1981 63 96 116 486
Cuts at: 6869 1391
Size: 4072
Fragments arranged by size:
4072 1996 1981 486 390 350 116 96
63
BbeI G_GCGC'C
Cuts at: 4996 6617 4996
Size: 1621 7929
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3923 3940 4100 5555 928
Size: 971 2024 17 160 1455 4923
Fragments arranged by size:
4923 2024 1455 971 160 17
BbvCI CC'TCA_GC
Cuts at: 4036 5090 4036
Size: 1054 8496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3253 3287 4345 4379 6145 6179 7741
Size: 34 1316 34 1058 34 1766 34 1562
Cuts at: 7741 7775 1903
Size: 34 3678
Fragments arranged by size:
3678 1766 1562 1316 1058 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5834 7442 8652 5834
Size: 1608 1210 6732
Fragments arranged by size:
6732 1608 1210
BclI T'GATC_A
Cuts at: 1548 3591 1548
Size: 2043 7507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3342 3891
Size: 107 122 71 631 605 337 1333 549
Cuts at: 3891 4707 8161 136
Size: 816 3454 1525
Fragments arranged by size:
3454 1525 1333 816 631 605 549 337
122 107 71
BglII A'GATC_T
Cuts at: 9545 9545
Size: 9550
BlpI GC'TnA_GC
Cuts at: 2651 2864 5162 6967 2651
Size: 213 2298 1805 5234
Fragments arranged by size:
5234 2298 1805 213
Bme1580I G_kGCm'C
Cuts at: 1790 2836 3314 3720 5659 6171 6267 6306 6417
Size: 1046 478 406 1939 512 96 39 111
Cuts at: 6417 7611 8767 1790
Size: 1194 1156 2573
Fragments arranged by size:
2573 1939 1194 1156 1046 512 478 406
111 96 39
BmgBI CAC'GTC
Cuts at: 1318 2248 3009 4003 5127 6436 6643 1318
Size: 930 761 994 1124 1309 207 4225
Fragments arranged by size:
4225 1309 1124 994 930 761 207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2362 2521 2644
Size: 416 53 83 399 24 945 159 123
Cuts at: 2644 2814 3286 3445 3568 4213 4224 4990 5381
Size: 170 472 159 123 645 11 766 391
Cuts at: 5381 5720 5902 7281 8239 442
Size: 339 182 1379 958 1753
Fragments arranged by size:
1753 1379 958 945 766 645 472 416
399 391 339 182 170 159 159 123
123 83 53 24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5656 5656
Size: 9550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2210 2636 3647 3917 5471 5651 8210
Size: 944 27 426 1011 270 1554 180 2559
Cuts at: 8210 1239
Size: 2579
Fragments arranged by size:
2579 2559 1554 1011 944 426 270 180
27
Bpu10I CC'TnA_GC
Cuts at: 4036 5090 4036
Size: 1054 8496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7675 8540 8838 7675
Size: 865 298 8387
Fragments arranged by size:
8387 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2332 2381 3980 6574 8213 882
Size: 617 833 49 1599 2594 1639 2219
Fragments arranged by size:
2594 2219 1639 1599 833 617 49
BsaAI yAC'GTr
Cuts at: 493 1204 4138 6301 7341 493
Size: 711 2934 2163 1040 2702
Fragments arranged by size:
2934 2702 2163 1040 711
BsaBI GATnn'nnATC
Cuts at: 4676 9544 4676
Size: 4868 4682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2522 2643 3567
Size: 53 83 186 936 612 377 121 924
Cuts at: 3567 3741 4023 4083 4993 5457 6003 6614 6626
Size: 174 282 60 910 464 546 611 12
Cuts at: 6626 7357 7739 8374 275
Size: 731 382 635 1451
Fragments arranged by size:
1451 936 924 910 731 635 612 611
546 464 382 377 282 186 174 121
83 60 53 12
BsaWI w'CCGG_w
Cuts at: 6027 6426 7983 8381 8655 8802 9213 6027
Size: 399 1557 398 274 147 411 6364
Fragments arranged by size:
6364 1557 411 399 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3332 3362 3962 3992 5225
Size: 30 165 30 1929 30 600 30 1233
Cuts at: 5225 5255 1178
Size: 30 5473
Fragments arranged by size:
5473 1929 1233 600 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2633 2918 3041 4684 5648 5894 6811
Size: 1084 363 285 123 1643 964 246 917
Cuts at: 6811 1186
Size: 3925
Fragments arranged by size:
3925 1643 1084 964 917 363 285 246
123
BseYI C'CCAG_C
Cuts at: 3664 4504 4811 5028 6333 8753 9249 3664
Size: 840 307 217 1305 2420 496 3965
Fragments arranged by size:
3965 2420 1305 840 496 307 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 3005 3386 3859 4583 5489 6820
Size: 573 585 684 381 473 724 906 1331
Cuts at: 6820 1163
Size: 3893
Fragments arranged by size:
3893 1331 906 724 684 585 573 473
381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2202 3273 4633 5518 6427 7000
Size: 275 186 201 1071 1360 885 909 573
Cuts at: 7000 7761 8789 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1360 1071 1028 909 885 761 573
275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2439 3720 4127 4137 5720 5742 5900 6108 6383
Size: 1281 407 10 1583 22 158 208 275
Cuts at: 6383 7611 7696 8767 2439
Size: 1228 85 1071 3222
Fragments arranged by size:
3222 1583 1281 1228 1071 407 275 208
158 85 22 10
BsiWI C'GTAC_G
Cuts at: 2131 2131
Size: 9550
BsmI GAATG_Cn'
Cuts at: 7061 7154 7061
Size: 93 9457
BsmBI CGTCTCn'nnnn_
Cuts at: 2513 3558 2513
Size: 1045 8505
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2836 3314 3387 3720 3777 4127
Size: 399 649 397 478 73 333 57 350
Cuts at: 4127 4137 5659 5720 5742 5900 6108 6171 6267
Size: 10 1522 61 22 158 208 63 96
Cuts at: 6267 6306 6383 6417 6869 7611 7696 8767 1391
Size: 39 77 34 452 742 85 1071 2174
Fragments arranged by size:
2174 1522 1071 742 649 478 452 399
397 350 333 208 158 96 85 77
73 63 61 57 39 34 22 10
BspEI T'CCGG_A
Cuts at: 9213 9213
Size: 9550
BspHI T'CATG_A
Cuts at: 4113 7439 9169 4113
Size: 3326 1730 4494
Fragments arranged by size:
4494 3326 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3097 3546 3929 5760 5786 5949 6905 844
Size: 2253 449 383 1831 26 163 956 3489
Fragments arranged by size:
3489 2253 1831 956 449 383 163 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2486 2536 2778 2875 3484 3712
Size: 429 281 382 50 242 97 609 228
Cuts at: 3712 4015 4516 4612 5166 5982 6039 7437 1394
Size: 303 501 96 554 816 57 1398 3507
Fragments arranged by size:
3507 1398 816 609 554 501 429 382
303 281 242 228 97 96 57 50
BsrDI GCAATG_nn'
Cuts at: 65 1248 3129 8045 8219 65
Size: 1183 1881 4916 174 1396
Fragments arranged by size:
4916 1881 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 5330 8194 8381 1700
Size: 110 337 3183 2864 187 2869
Fragments arranged by size:
3183 2869 2864 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 6534 96
Size: 6438 3112
BssHII G'CGCG_C
Cuts at: 1925 5919 1925
Size: 3994 5556
BssSI C'ACGA_G
Cuts at: 4268 5735 6275 7610 8622 4268
Size: 1467 540 1335 1012 5196
Fragments arranged by size:
5196 1467 1335 1012 540
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5752 6360 794
Size: 4958 608 3984
Fragments arranged by size:
4958 3984 608
BstBI TT'CG_AA
Cuts at: 8354 8354
Size: 9550
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9550
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2476 2614 3526 3895 4504 5182 5600 2176
Size: 300 138 912 369 609 678 418 6126
Fragments arranged by size:
6126 912 678 609 418 369 300 138
BstYI r'GATC_y
Cuts at: 1972 2823 3066 3357 3975 4309 4906 6880 7247
Size: 851 243 291 618 334 597 1974 367
Cuts at: 7247 7632 7649 9101 9187 9199 9545 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1974 1452 851 618 597 385 367
346 334 291 243 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3933 3933
Size: 9550
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 2091 2847 2976 5175 5711
Size: 552 939 26 61 756 129 2199 536
Cuts at: 5711 6519 513
Size: 808 3544
Fragments arranged by size:
3544 2199 939 808 756 552 536 129
61 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 3250 3379 5877 6340 487
Size: 683 56 1229 795 129 2498 463 3697
Fragments arranged by size:
3697 2498 1229 795 683 463 129 56
BtsI GCAGTG_nn'
Cuts at: 2211 3398 4745 5619 6071 6163 6573 7066 7860
Size: 1187 1347 874 452 92 410 493 794
Cuts at: 7860 7880 2211
Size: 20 3881
Fragments arranged by size:
3881 1347 1187 874 794 493 452 410
92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9515
DraI TTT'AAA
Cuts at: 6854 6854
Size: 9550
DraIII CAC_nnn'GTG
Cuts at: 3409 4141 5737 3409
Size: 732 1596 7222
Fragments arranged by size:
7222 1596 732
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8557 809
Size: 507 7241 1802
Fragments arranged by size:
7241 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2199 2406
Size: 54 1425 50 275 18 203 166 207
Cuts at: 2406 3792 4005 4173 4630 4771 6040 6516 6570
Size: 1386 213 168 457 141 1269 476 54
Cuts at: 6570 6997 7886 9515 8
Size: 427 889 1629 43
Fragments arranged by size:
1629 1425 1386 1269 889 476 457 427
275 213 207 203 168 166 141 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2199 4630 6997 1537
Size: 275 387 2431 2367 4090
Fragments arranged by size:
4090 2431 2367 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2807 3605 5327 6200 6562 6818 7480
Size: 1091 363 798 1722 873 362 256 662
Cuts at: 7480 1353
Size: 3423
Fragments arranged by size:
3423 1722 1091 873 798 662 363 362
256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2166 3064 3631 5330 5890 6008
Size: 1064 138 577 898 567 1699 560 118
Cuts at: 6008 6776 8137 8511 8657 387
Size: 768 1361 374 146 1280
Fragments arranged by size:
1699 1361 1280 1064 898 768 577 567
560 374 146 138 118
EcoICRI GAG'CTC
Cuts at: 4125 6106 6381 4125
Size: 1981 275 7294
Fragments arranged by size:
7294 1981 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2210 2636 3647 3917 4112 5423
Size: 393 551 27 426 1011 270 195 1311
Cuts at: 5423 5471 5651 7613 8210 8997 9265 1239
Size: 48 180 1962 597 787 268 1524
Fragments arranged by size:
1962 1524 1311 1011 787 597 551 426
393 270 268 195 180 48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3542 3626 4649 3542
Size: 84 1023 8443
Fragments arranged by size:
8443 1023 84
EcoO109I rG'GnC_Cy
Cuts at: 2449 3373 5025 5317 5832 6264 6291 6444 6591
Size: 924 1652 292 515 432 27 153 147
Cuts at: 6591 2449
Size: 5408
Fragments arranged by size:
5408 1652 924 515 432 292 153 147
27
EcoRI G'AATT_C
Cuts at: 6859 7237 6859
Size: 378 9172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2671 2970 2455
Size: 216 299 9035
Fragments arranged by size:
9035 299 216
FspI TGC'GCA
Cuts at: 8056 8056
Size: 9550
HaeII r_GCGC'y
Cuts at: 1678 2018 2069 3700 4456 4996 5842 6247 6617
Size: 340 51 1631 756 540 846 405 370
Cuts at: 6617 6666 8697 1678
Size: 49 2031 2531
Fragments arranged by size:
2531 2031 1631 846 756 540 405 370
340 51 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2592 2624 3951 3983 6689
Size: 32 135 32 1020 32 1327 32 2706
Cuts at: 6689 6721 8191 8223 8265 8297 1373
Size: 32 1470 32 42 32 2626
Fragments arranged by size:
2706 2626 1470 1327 1020 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 3274 4180 5227 6894 7140
Size: 988 281 1300 36 906 1047 1667 246
Cuts at: 7140 669
Size: 3079
Fragments arranged by size:
3079 1667 1300 1047 988 906 281 246
36
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9550
HpaI GTT'AAC
Cuts at: 7140 7140
Size: 9550
KasI G'GCGC_C
Cuts at: 4992 6613 4992
Size: 1621 7929
KpnI G_GTAC'C
Cuts at: 5245 6875 5245
Size: 1630 7920
MfeI C'AATT_G
Cuts at: 7149 7149
Size: 9550
MluI A'CGCG_T
Cuts at: 9426 9426
Size: 9550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3348 3784 4083 4521 4856 6123 8664 8848
Size: 2043 436 299 438 335 1267 2541 184
Cuts at: 8848 1305
Size: 2007
Fragments arranged by size:
2541 2043 2007 1267 438 436 335 299
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 3794 4007 10
Size: 54 1425 343 576 1386 213 5553
Fragments arranged by size:
5553 1425 1386 576 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2402 2858 2981 3326 4481 4682
Size: 648 1008 228 456 123 345 1155 201
Cuts at: 4682 5180 5258 5652 5713 6156 6279 6545 6641
Size: 498 78 394 61 443 123 266 96
Cuts at: 6641 7508 7867 8026 518
Size: 867 359 159 2042
Fragments arranged by size:
2042 1155 1008 867 648 498 456 443
394 359 345 266 228 201 159 123
123 96 78 61
MspA1I CmG'CkG
Cuts at: 1606 2038 2093 2184 2989 3757 4750 4779 6288
Size: 432 55 91 805 768 993 29 1509
Cuts at: 6288 6672 6771 6966 7643 8791 9036 1606
Size: 384 99 195 677 1148 245 2120
Fragments arranged by size:
2120 1509 1148 993 805 768 677 432
384 245 195 99 91 55 29
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 9550
NarI GG'CG_CC
Cuts at: 4993 6614 4993
Size: 1621 7929
NcoI C'CATG_G
Cuts at: 513 1065 2004 2847 5175 513
Size: 552 939 843 2328 4888
Fragments arranged by size:
4888 2328 939 843 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9550
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 9550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9550
NotI GC'GGCC_GC
Cuts at: 2199 6997 2199
Size: 4798 4752
NspI r_CATG'y
Cuts at: 1132 1229 2500 2967 4504 5263 5802 6363 6550
Size: 97 1271 467 1537 759 539 561 187
Cuts at: 6550 6908 8453 1132
Size: 358 1545 2229
Fragments arranged by size:
2229 1545 1537 1271 759 561 539 467
358 187 97
PasI CC'CwG_GG
Cuts at: 1348 5211 1348
Size: 3863 5687
PciI A'CATG_T
Cuts at: 2496 6546 8449 2496
Size: 4050 1903 3597
Fragments arranged by size:
4050 3597 1903
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5614 6232 6672 1108
Size: 4506 618 440 3986
Fragments arranged by size:
4506 3986 618 440
PfoI T'CCnGG_A
Cuts at: 2426 3624 4884 2426
Size: 1198 1260 7092
Fragments arranged by size:
7092 1260 1198
PmeI GTTT'AAAC
Cuts at: 6854 6854
Size: 9550
PmlI CAC'GTG
Cuts at: 4138 6301 4138
Size: 2163 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 6695 6727 7619 7651 9132 9164 4109
Size: 32 2554 32 892 32 1481 32 4495
Fragments arranged by size:
4495 2554 1481 892 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 3373 5025 5317 6291 2449
Size: 924 1652 292 974 5708
Fragments arranged by size:
5708 1652 974 924 292
PshAI GACnn'nnGTC
Cuts at: 1613 3233 4082 1613
Size: 1620 849 7081
Fragments arranged by size:
7081 1620 849
PsiI TTA'TAA
Cuts at: 7120 7120
Size: 9550
PspOMI G'GGCC_C
Cuts at: 6167 6263 6167
Size: 96 9454
PstI C_TGCA'G
Cuts at: 830 2994 3108 3543 4829 5748 6635 6902 830
Size: 2164 114 435 1286 919 887 267 3478
Fragments arranged by size:
3478 2164 1286 919 887 435 267 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9550
PvuII CAG'CTG
Cuts at: 2989 3757 4750 6771 2989
Size: 768 993 2021 5768
Fragments arranged by size:
5768 2021 993 768
RsrII CG'GwC_CG
Cuts at: 2024 3316 2024
Size: 1292 8258
SacI G_AGCT'C
Cuts at: 4127 6108 6383 4127
Size: 1981 275 7294
Fragments arranged by size:
7294 1981 275
SacII CC_GC'GG
Cuts at: 2094 2094
Size: 9550
SalI G'TCGA_C
Cuts at: 1936 3272 6892 1936
Size: 1336 3620 4594
Fragments arranged by size:
4594 3620 1336
SanDI GG'GwC_CC
Cuts at: 5025 6291 5025
Size: 1266 8284
SapI GCTCTTCn'nnn_
Cuts at: 2444 2444
Size: 9550
SbfI CC_TGCA'GG
Cuts at: 3543 6902 3543
Size: 3359 6191
ScaI AGT'ACT
Cuts at: 6689 7798 9510 6689
Size: 1109 1712 6729
Fragments arranged by size:
6729 1712 1109
SfcI C'TryA_G
Cuts at: 826 1045 2990 3104 3539 4825 5104 5744 6631
Size: 219 1945 114 435 1286 279 640 887
Cuts at: 6631 6658 6898 8033 8714 8905 826
Size: 27 240 1135 681 191 1471
Fragments arranged by size:
1945 1471 1286 1135 887 681 640 435
279 240 219 191 114 27
SfoI GGC'GCC
Cuts at: 4994 6615 4994
Size: 1621 7929
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9550
SgrAI Cr'CCGG_yG
Cuts at: 1700 2147 1700
Size: 447 9103
SmaI CCC'GGG
Cuts at: 1872 5830 6324 6877 1872
Size: 3958 494 553 4545
Fragments arranged by size:
4545 3958 553 494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3983 7654 8555 8817 820
Size: 197 926 2040 3671 901 262 1553
Fragments arranged by size:
3671 2040 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7341 493
Size: 6848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9550
SphI G_CATG'C
Cuts at: 1132 6363 6908 1132
Size: 5231 545 3774
Fragments arranged by size:
5231 3774 545
SspI AAT'ATT
Cuts at: 5 52 7474 5
Size: 47 7422 2081
Fragments arranged by size:
7422 2081 47
StuI AGG'CCT
Cuts at: 1517 3938 4034 1517
Size: 2421 96 7033
Fragments arranged by size:
7033 2421 96
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 2475 2847 3795 4317 5013
Size: 552 939 219 252 372 948 522 696
Cuts at: 5013 5056 5175 5491 5694 6989 513
Size: 43 119 316 203 1295 3074
Fragments arranged by size:
3074 1295 948 939 696 552 522 372
316 252 219 203 119 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2860 3268 3317 4352 4481 4541
Size: 1255 764 516 408 49 1035 129 60
Cuts at: 4541 4972 5377 5664 6194 6748 7577 7594 7747
Size: 431 405 287 530 554 829 17 153
Cuts at: 7747 7932 8377 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1035 829 764 554 530 516
445 431 408 405 287 185 153 129
60 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2370 2499
Size: 274 80 33 51 1443 258 135 129
Cuts at: 2499 2754 3423 4563 5247 6534 6687 7796 9508
Size: 255 669 1140 684 1287 153 1109 1712
Cuts at: 9508 96
Size: 138
Fragments arranged by size:
1712 1443 1287 1140 1109 684 669 274
258 255 153 138 135 129 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3962 3994 9510 9542 3962
Size: 32 5516 32 3970
Fragments arranged by size:
5516 3970 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9550
XbaI T'CTAG_A
Cuts at: 6886 6886
Size: 9550
XhoI C'TCGA_G
Cuts at: 1943 3983 1943
Size: 2040 7510
XmaI C'CCGG_G
Cuts at: 1870 5828 6322 6875 1870
Size: 3958 494 553 4545
Fragments arranged by size:
4545 3958 553 494
XmnI GAAnn'nnTTC
Cuts at: 7679 7679
Size: 9550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3742 4084 7358 276
Size: 53 83 186 936 2208 342 3274 2468
Fragments arranged by size:
3274 2468 2208 936 342 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoRV FseI FspAI
NruI NsiI PacI PspXI PsrI SexAI SfiI SrfI
SwaI XcmI