Restriction map for
pFN21AB3238
(Circular) (Six-base) MAPSORT of: pFN21AB3238.seq Check: 4779 from: 1 to: 6901
pFN21AB3238 6901 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4711 278
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4244 1937
Size: 2307 4594
Acc65I G'GTAC_C
Cuts at: 4222 4222
Size: 6901
AclI AA'CG_TT
Cuts at: 1563 5028 5401 1563
Size: 3465 373 3063
Fragments arranged by size:
3465 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3715 3771 4964 6348 6616 1632
Size: 1302 781 56 1193 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1193 781 268 56
AfeI AGC'GCT
Cuts at: 3965 3965
Size: 6901
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6704
AflIII A'CryG_T
Cuts at: 1203 3303 5800 6777 1203
Size: 2100 2497 977 1327
Fragments arranged by size:
2497 2100 1327 977
AgeI A'CCGG_T
Cuts at: 5732 5732
Size: 6901
AhdI GACnn_n'nnGTC
Cuts at: 5630 5630
Size: 6901
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6901
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3069 3101 3069
Size: 32 6869
AlwNI CAG_nnn'CTG
Cuts at: 3484 4154 4644 6216 3484
Size: 670 490 1572 4169
Fragments arranged by size:
4169 1572 670 490
ApaLI G'TGCA_C
Cuts at: 2240 4958 6114 2240
Size: 2718 1156 3027
Fragments arranged by size:
3027 2718 1156
ApoI r'AATT_y
Cuts at: 1493 2673 3660 4210 4436 4588 6697 1493
Size: 1180 987 550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1180 987 550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5455 160
Size: 872 4423 1606
Fragments arranged by size:
4423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
AvaI C'yCGr_G
Cuts at: 1870 1943 3507 4226 1870
Size: 73 1564 719 4545
Fragments arranged by size:
4545 1564 719 73
BamHI G'GATC_C
Cuts at: 4231 4598 4231
Size: 367 6534
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4222 5677 618
Size: 325 217 627 2435 1455 1842
Fragments arranged by size:
2435 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3727 4220 1391
Size: 2336 493 4072
Fragments arranged by size:
4072 2336 493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 3294 3416 3432 928
Size: 971 805 590 122 16 4397
Fragments arranged by size:
4397 971 805 590 122 16
BbvCI CC'TCA_GC
Cuts at: 2417 3564 4098 2417
Size: 1147 534 5220
Fragments arranged by size:
5220 1147 534
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5092 5126 1903
Size: 34 3155 34 3678
Fragments arranged by size:
3678 3155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3871 4793 6003 3871
Size: 922 1210 4769
Fragments arranged by size:
4769 1210 922
BclI T'GATC_A
Cuts at: 1548 4059 1548
Size: 2511 4390
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5512 136
Size: 107 122 71 631 605 3840 1525
Fragments arranged by size:
3840 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6896 6896
Size: 6901
BlpI GC'TnA_GC
Cuts at: 4318 4318
Size: 6901
Bme1580I G_kGCm'C
Cuts at: 1790 2244 4962 6118 1790
Size: 454 2718 1156 2573
Fragments arranged by size:
2718 2573 1156 454
BmgBI CAC'GTC
Cuts at: 1318 2050 1318
Size: 732 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4632 5590 442
Size: 416 53 83 399 24 3215 958 1753
Fragments arranged by size:
3215 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4093 4093
Size: 6901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 3724 5561 1239
Size: 1058 1427 1837 2579
Fragments arranged by size:
2579 1837 1427 1058
Bpu10I CC'TnA_GC
Cuts at: 2417 2557 3564 3654 4098 2417
Size: 140 1007 90 444 5220
Fragments arranged by size:
5220 1007 444 140 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3198 5026 5891 6189 2639
Size: 559 1828 865 298 3351
Fragments arranged by size:
3351 1828 865 559 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5564 882
Size: 617 4065 2219
Fragments arranged by size:
4065 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4692 493
Size: 711 3488 2702
Fragments arranged by size:
3488 2702 711
BsaBI GATnn'nnATC
Cuts at: 2223 6895 2223
Size: 4672 2229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4708 5090 5725 275
Size: 53 83 186 936 3175 382 635 1451
Fragments arranged by size:
3175 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5334 5732 6006 6153 6564 5334
Size: 398 274 147 411 5671
Fragments arranged by size:
5671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3002 3032 1178
Size: 30 165 30 1599 30 5047
Fragments arranged by size:
5047 1599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2553 1186
Size: 1367 5534
BseYI C'CCAG_C
Cuts at: 6104 6600 6104
Size: 496 6405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3290 3473 1163
Size: 573 1554 183 4591
Fragments arranged by size:
4591 1554 573 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 4351 5112 6140 1540
Size: 275 186 14 2336 761 1028 2301
Fragments arranged by size:
2336 2301 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2244 4962 5047 6118 2244
Size: 2718 85 1071 3027
Fragments arranged by size:
3027 2718 1071 85
BsmI GAATG_Cn'
Cuts at: 2731 3364 4412 4505 2731
Size: 633 1048 93 5127
Fragments arranged by size:
5127 1048 633 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3742 3742
Size: 6901
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 3727 4220 4962 5047 6118 1391
Size: 399 454 1483 493 742 85 1071 2174
Fragments arranged by size:
2174 1483 1071 742 493 454 399 85
BspEI T'CCGG_A
Cuts at: 6564 6564
Size: 6901
BspHI T'CATG_A
Cuts at: 4790 6520 4790
Size: 1730 5171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4256 844
Size: 3412 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4788 1394
Size: 429 2965 3507
Fragments arranged by size:
3507 2965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5396 5570 65
Size: 1183 4148 174 1396
Fragments arranged by size:
4148 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5545 5732 1700
Size: 110 3735 187 2869
Fragments arranged by size:
3735 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2128 96
Size: 2032 4869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6901
BssSI C'ACGA_G
Cuts at: 4961 5973 4961
Size: 1012 5889
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3915 794
Size: 3121 3780
BstBI TT'CG_AA
Cuts at: 5705 5705
Size: 6901
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6901
BstXI CCAn_nnnn'nTGG
Cuts at: 3391 3683 3391
Size: 292 6609
BstYI r'GATC_y
Cuts at: 1972 4231 4598 4983 5000 6452 6538 6550 6896
Size: 2259 367 385 17 1452 86 12 346
Cuts at: 6896 1972
Size: 1977
Fragments arranged by size:
2259 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2566 513
Size: 552 939 562 4848
Fragments arranged by size:
4848 939 562 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6162
Fragments arranged by size:
6162 683 56
BtsI GCAGTG_nn'
Cuts at: 3763 4417 5211 5231 3763
Size: 654 794 20 5433
Fragments arranged by size:
5433 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 2801 1554
Size: 1247 5654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4120 4155 529
Size: 35 3556 35 3275
Fragments arranged by size:
3556 3275 35 35
DraI TTT'AAA
Cuts at: 2922 4205 2922
Size: 1283 5618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5908 809
Size: 507 4592 1802
Fragments arranged by size:
4592 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2563 3174 4348
Size: 54 1425 50 275 18 733 611 1174
Cuts at: 4348 5237 6866 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1174 889 733 611 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4348 1537
Size: 275 2536 4090
Fragments arranged by size:
4090 2536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 3899 3951 4831 1353
Size: 1031 1515 52 880 3423
Fragments arranged by size:
3423 1515 1031 880 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5488 5862 6008 387
Size: 1064 138 3899 374 146 1280
Fragments arranged by size:
3899 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2934 3715 3724 3771 4964 5561
Size: 393 665 637 781 9 47 1193 597
Cuts at: 5561 6348 6616 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1193 787 781 665 637 597 393
268 47 9
EcoRI G'AATT_C
Cuts at: 4210 4588 4210
Size: 378 6523
EcoRV GAT'ATC
Cuts at: 2643 2643
Size: 6901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2633 3844 2633
Size: 1211 5690
FspI TGC'GCA
Cuts at: 5407 5407
Size: 6901
HaeII r_GCGC'y
Cuts at: 1678 3967 6048 1678
Size: 2289 2081 2531
Fragments arranged by size:
2531 2289 2081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5542 5574 5616 5648 1373
Size: 32 135 32 3970 32 42 32 2626
Fragments arranged by size:
3970 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4245 4491 669
Size: 988 281 2307 246 3079
Fragments arranged by size:
3079 2307 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6901
HpaI GTT'AAC
Cuts at: 4491 4491
Size: 6901
KpnI G_GTAC'C
Cuts at: 4226 4226
Size: 6901
MfeI C'AATT_G
Cuts at: 4500 4500
Size: 6901
MluI A'CGCG_T
Cuts at: 6777 6777
Size: 6901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2323 2646 3461 3751 6015 6199 1305
Size: 1018 323 815 290 2264 184 2007
Fragments arranged by size:
2264 2007 1018 815 323 290 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2565 3176 10
Size: 54 1425 343 733 611 3735
Fragments arranged by size:
3735 1425 733 611 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2351 3249 3681 4859 5218 5377
Size: 648 1071 114 898 432 1178 359 159
Cuts at: 5377 518
Size: 2042
Fragments arranged by size:
2042 1178 1071 898 648 432 359 159
114
MspA1I CmG'CkG
Cuts at: 1606 2030 2443 3122 3274 3563 4097 4154 4317
Size: 424 413 679 152 289 534 57 163
Cuts at: 4317 4994 6142 6387 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 534 424 413 289
245 163 152 57
NcoI C'CATG_G
Cuts at: 513 1065 2004 2566 513
Size: 552 939 562 4848
Fragments arranged by size:
4848 939 562 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6901
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6901
NotI GC'GGCC_GC
Cuts at: 4348 4348
Size: 6901
NspI r_CATG'y
Cuts at: 1132 1229 3307 4259 5804 1132
Size: 97 2078 952 1545 2229
Fragments arranged by size:
2229 2078 1545 952 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6901
PciI A'CATG_T
Cuts at: 3303 5800 3303
Size: 2497 4404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2742 1108
Size: 1634 5267
PfoI T'CCnGG_A
Cuts at: 2325 3930 4041 2325
Size: 1605 111 5185
Fragments arranged by size:
5185 1605 111
PmeI GTTT'AAAC
Cuts at: 4205 4205
Size: 6901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4970 5002 6483 6515 4970
Size: 32 1481 32 5356
Fragments arranged by size:
5356 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6901
PsiI TTA'TAA
Cuts at: 2453 4471 2453
Size: 2018 4883
PstI C_TGCA'G
Cuts at: 830 3127 4253 830
Size: 2297 1126 3478
Fragments arranged by size:
3478 2297 1126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6901
PvuII CAG'CTG
Cuts at: 2030 2443 3122 3274 4097 4154 2030
Size: 413 679 152 823 57 4777
Fragments arranged by size:
4777 823 679 413 152 57
SalI G'TCGA_C
Cuts at: 1936 4243 1936
Size: 2307 4594
SapI GCTCTTCn'nnn_
Cuts at: 3899 3951 3899
Size: 52 6849
SbfI CC_TGCA'GG
Cuts at: 4253 4253
Size: 6901
ScaI AGT'ACT
Cuts at: 5149 6861 5149
Size: 1712 5189
SexAI A'CCwGG_T
Cuts at: 2302 2302
Size: 6901
SfcI C'TryA_G
Cuts at: 826 1045 2807 3123 3690 4249 5384 6065 6256
Size: 219 1762 316 567 559 1135 681 191
Cuts at: 6256 826
Size: 1471
Fragments arranged by size:
1762 1471 1135 681 567 559 316 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6901
SmaI CCC'GGG
Cuts at: 1872 3509 4228 1872
Size: 1637 719 4545
Fragments arranged by size:
4545 1637 719
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3213 5005 5906 6168 820
Size: 197 926 675 595 1792 901 262 1553
Fragments arranged by size:
1792 1553 926 901 675 595 262 197
SnaBI TAC'GTA
Cuts at: 493 4692 493
Size: 4199 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6901
SphI G_CATG'C
Cuts at: 1132 4259 1132
Size: 3127 3774
SspI AAT'ATT
Cuts at: 5 52 2406 4825 5
Size: 47 2354 2419 2081
Fragments arranged by size:
2419 2354 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6901
StyI C'CwwG_G
Cuts at: 513 1065 2004 2566 4340 513
Size: 552 939 562 1774 3074
Fragments arranged by size:
3074 1774 939 562 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4928 4945 5098 5283 5728 325
Size: 1255 3348 17 153 185 445 1498
Fragments arranged by size:
3348 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2128 5147 6859
Size: 274 80 33 51 1443 151 3019 1712
Cuts at: 6859 96
Size: 138
Fragments arranged by size:
3019 1712 1443 274 151 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2185 2217 3002 3034 6861 6893 2185
Size: 32 785 32 3827 32 2193
Fragments arranged by size:
3827 2193 785 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6901
XbaI T'CTAG_A
Cuts at: 4237 4237
Size: 6901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4114 4114
Size: 6901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6901
XmaI C'CCGG_G
Cuts at: 1870 3507 4226 1870
Size: 1637 719 4545
Fragments arranged by size:
4545 1637 719
XmnI GAAnn'nnTTC
Cuts at: 3020 5030 3020
Size: 2010 4891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4709 276
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI