Restriction map for
pFN21AB3236
(Circular) (Six-base) MAPSORT of: pFN21AB3236.seq Check: 5797 from: 1 to: 6421
pFN21AB3236 6421 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4231 278
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3764 1937
Size: 1827 4594
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 3705 4484 5868 6136 1632
Size: 759 1314 779 1384 268 1917
Fragments arranged by size:
1917 1384 1314 779 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 5320 6297 1203
Size: 4117 977 1327
Fragments arranged by size:
4117 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3277 1184
Size: 2093 4328
AlwNI CAG_nnn'CTG
Cuts at: 2828 4164 5736 2828
Size: 1336 1572 3513
Fragments arranged by size:
3513 1572 1336
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 2185 2291 2684 3171 3730 3956 4108 6217
Size: 692 106 393 487 559 226 152 2109
Cuts at: 6217 1493
Size: 1697
Fragments arranged by size:
2109 1697 692 559 487 393 226 152
106
AseI AT'TA_AT
Cuts at: 160 1032 3020 4975 160
Size: 872 1988 1955 1606
Fragments arranged by size:
1988 1955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 3746 1870
Size: 73 1803 4545
Fragments arranged by size:
4545 1803 73
AvrII C'CTAG_G
Cuts at: 2923 2923
Size: 6421
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3742 5197 618
Size: 325 217 627 1955 1455 1842
Fragments arranged by size:
1955 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3740 1391
Size: 622 1727 4072
Fragments arranged by size:
4072 1727 622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5450
BbvCI CC'TCA_GC
Cuts at: 3450 3450
Size: 6421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 2884 1548
Size: 1336 5085
BfrBI ATG'CAT
Cuts at: 2420 2759 3566 2420
Size: 339 807 5275
Fragments arranged by size:
5275 807 339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5032 136
Size: 107 122 71 631 605 3360 1525
Fragments arranged by size:
3360 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 3462 3838 3462
Size: 376 6045
Bme1580I G_kGCm'C
Cuts at: 1790 4482 5638 1790
Size: 2692 1156 2573
Fragments arranged by size:
2692 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3035 3284 1318
Size: 1717 249 4455
Fragments arranged by size:
4455 1717 249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 3402 5081 1239
Size: 1692 471 1679 2579
Fragments arranged by size:
2579 1692 1679 471
Bpu10I CC'TnA_GC
Cuts at: 3450 3450
Size: 6421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2544 4546 5411 5709 2544
Size: 2002 865 298 3256
Fragments arranged by size:
3256 2002 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 2478 2849 5084 882
Size: 617 876 103 371 2235 2219
Fragments arranged by size:
2235 2219 876 617 371 103
BsaAI yAC'GTr
Cuts at: 493 1204 4212 493
Size: 711 3008 2702
Fragments arranged by size:
3008 2702 711
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4228 4610 5245 275
Size: 53 83 186 936 2695 382 635 1451
Fragments arranged by size:
2695 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4854 5252 5526 5673 6084 4854
Size: 398 274 147 411 5191
Fragments arranged by size:
5191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6196
Fragments arranged by size:
6196 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 1186
Size: 1432 4989
BseYI C'CCAG_C
Cuts at: 5624 6120 5624
Size: 496 5925
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3713 1163
Size: 573 1977 3871
Fragments arranged by size:
3871 1977 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2392 3871 4632 5660 1540
Size: 275 186 391 1479 761 1028 2301
Fragments arranged by size:
2301 1479 1028 761 391 275 186
BsiHKAI G_wGCw'C
Cuts at: 4482 4567 5638 4482
Size: 85 1071 5265
Fragments arranged by size:
5265 1071 85
BsmI GAATG_Cn'
Cuts at: 2761 3158 3932 4025 2761
Size: 397 774 93 5157
Fragments arranged by size:
5157 774 397 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2771 2771
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 3740 4482 4567 5638 1391
Size: 399 223 1727 742 85 1071 2174
Fragments arranged by size:
2174 1727 1071 742 399 223 85
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 4310 6040 4310
Size: 1730 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2831 3776 844
Size: 1217 770 945 3489
Fragments arranged by size:
3489 1217 945 770
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 4308 1394
Size: 429 1804 681 3507
Fragments arranged by size:
3507 1804 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4916 5090 65
Size: 1183 3668 174 1396
Fragments arranged by size:
3668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5065 5252 1700
Size: 110 3255 187 2869
Fragments arranged by size:
3255 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 4481 5493 4481
Size: 1012 5409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2721 2831 3293 794
Size: 1927 110 462 3922
Fragments arranged by size:
3922 1927 462 110
BstBI TT'CG_AA
Cuts at: 2860 5225 2860
Size: 2365 4056
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6421
BstXI CCAn_nnnn'nTGG
Cuts at: 2909 2909
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2160 2233 2617 3751 4118 4503 4520 5972
Size: 188 73 384 1134 367 385 17 1452
Cuts at: 5972 6058 6070 6416 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1134 385 384 367 346 188
86 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4930
Fragments arranged by size:
4930 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5682
Fragments arranged by size:
5682 683 56
BtsI GCAGTG_nn'
Cuts at: 3266 3937 4731 4751 3266
Size: 671 794 20 4936
Fragments arranged by size:
4936 794 671 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2423 3352 3868
Size: 54 1425 50 275 18 593 929 516
Cuts at: 3868 4757 6386 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 929 889 593 516 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2641 2862 3330 3483 3681 4351 1353
Size: 744 544 221 468 153 198 670 3423
Fragments arranged by size:
3423 744 670 544 468 221 198 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2391 2931 3402 3705 4484 5081 5868
Size: 393 759 540 471 303 779 597 787
Cuts at: 5868 6136 1239
Size: 268 1524
Fragments arranged by size:
1524 787 779 759 597 540 471 393
303 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 3354 2078
Size: 1276 5145
EcoRI G'AATT_C
Cuts at: 2684 3730 4108 2684
Size: 1046 378 4997
Fragments arranged by size:
4997 1046 378
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 5568 1678
Size: 3890 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3331 3363 5062
Size: 32 135 32 970 32 757 32 1699
Cuts at: 5062 5094 5136 5168 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1699 970 757 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3715 3765 4011 669
Size: 988 281 1777 50 246 3079
Fragments arranged by size:
3079 1777 988 281 246 50
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 2114 3147 4020 2114
Size: 1033 873 4515
Fragments arranged by size:
4515 1033 873
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3254 5535 5719 1305
Size: 1949 2281 184 2007
Fragments arranged by size:
2281 2007 1949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2425 3354 10
Size: 54 1425 343 593 929 3077
Fragments arranged by size:
3077 1425 929 593 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 2513 3075 4379 4738 4897 518
Size: 648 1284 63 562 1304 359 159 2042
Fragments arranged by size:
2042 1304 1284 648 562 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 3837 4514 5662 5907 1606
Size: 2231 677 1148 245 2120
Fragments arranged by size:
2231 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4930
Fragments arranged by size:
4930 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NsiI A_TGCA'T
Cuts at: 2422 2761 3568 2422
Size: 339 807 5275
Fragments arranged by size:
5275 807 339
NspI r_CATG'y
Cuts at: 1132 1229 3779 5324 1132
Size: 97 2550 1545 2229
Fragments arranged by size:
2550 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6421
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6421
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3159 3191 4490 4522 6003 6035 3159
Size: 32 1299 32 1481 32 3545
Fragments arranged by size:
3545 1481 1299 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PstI C_TGCA'G
Cuts at: 830 3256 3773 830
Size: 2426 517 3478
Fragments arranged by size:
3478 2426 517
PvuI CG_AT'CG
Cuts at: 2001 2392 2001
Size: 391 6030
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SapI GCTCTTCn'nnn_
Cuts at: 2862 2862
Size: 6421
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 3643 4669 6381 3643
Size: 1026 1712 3683
Fragments arranged by size:
3683 1712 1026
SexAI A'CCwGG_T
Cuts at: 3717 3717
Size: 6421
SfcI C'TryA_G
Cuts at: 826 1045 3252 3769 4904 5585 5776 826
Size: 219 2207 517 1135 681 191 1471
Fragments arranged by size:
2207 1471 1135 681 517 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2559 4525 5426 5688 820
Size: 197 926 616 1966 901 262 1553
Fragments arranged by size:
1966 1553 926 901 616 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 3433 4345 5
Size: 47 3381 912 2081
Fragments arranged by size:
3381 2081 912 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6421
StyI C'CwwG_G
Cuts at: 513 1065 2004 2488 2902 2923 2952 3276 3355
Size: 552 939 484 414 21 29 324 79
Cuts at: 3355 3860 513
Size: 505 3074
Fragments arranged by size:
3074 939 552 505 484 414 324 79
29 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 4448 4465 4618 4803 5248 325
Size: 1255 471 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 471 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3641 4667 6379
Size: 274 80 33 51 1443 1664 1026 1712
Cuts at: 6379 96
Size: 138
Fragments arranged by size:
1712 1664 1443 1026 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3577 3609 6381 6413 3577
Size: 32 2772 32 3585
Fragments arranged by size:
3585 2772 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6421
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 6421
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 3171 4550 3171
Size: 1379 5042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4229 276
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI