Restriction map for
pFN21AB3233
(Circular) (Six-base) MAPSORT of: pFN21AB3233.seq Check: 6849 from: 1 to: 7045
pFN21AB3233 7045 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4388 1937
Size: 2451 4594
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 3137 3601 4202 4272 5108 6492 6760
Size: 587 918 464 601 70 836 1384 268
Cuts at: 6760 1632
Size: 1917
Fragments arranged by size:
1917 1384 918 836 601 587 464 268
70
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 5944 6921 1203
Size: 4741 977 1327
Fragments arranged by size:
4741 1327 977
AgeI A'CCGG_T
Cuts at: 2135 5876 2135
Size: 3741 3304
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3317 1184
Size: 2133 4912
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 2749
Size: 32 7013
AlwNI CAG_nnn'CTG
Cuts at: 3215 3686 4175 4788 6360 3215
Size: 471 489 613 1572 3900
Fragments arranged by size:
3900 1572 613 489 471
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2934 3101 4024 4354 4580 4732 6841 1493
Size: 1441 167 923 330 226 152 2109 1697
Fragments arranged by size:
2109 1697 1441 923 330 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 4370 1870
Size: 73 934 1493 4545
Fragments arranged by size:
4545 1493 934 73
BamHI G'GATC_C
Cuts at: 4375 4742 4375
Size: 367 6678
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2516 3689 4043 4366
Size: 325 217 627 351 378 1173 354 323
Cuts at: 4366 5821 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1173 627 378 354 351 325
323 217
BanII G_rGCy'C
Cuts at: 1391 3235 3558 3801 4007 4156 4364 1391
Size: 1844 323 243 206 149 208 4072
Fragments arranged by size:
4072 1844 323 243 208 206 149
BbeI G_GCGC'C
Cuts at: 4047 4047
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2140 2841 4153 928
Size: 971 241 701 1312 3820
Fragments arranged by size:
3820 1312 971 701 241
BbvCI CC'TCA_GC
Cuts at: 2793 2793
Size: 7045
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2636 4937 6147 2636
Size: 2301 1210 3534
Fragments arranged by size:
3534 2301 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3393 5656 136
Size: 107 122 71 631 605 1721 2263 1525
Fragments arranged by size:
2263 1721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7040 7040
Size: 7045
BlpI GC'TnA_GC
Cuts at: 4176 4462 4176
Size: 286 6759
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2266 5106 6262 1790
Size: 353 123 2840 1156 2573
Fragments arranged by size:
2840 2573 1156 353 123
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2382 2692 2728
Size: 416 53 83 399 24 965 310 36
Cuts at: 2728 3157 4776 5734 442
Size: 429 1619 958 1753
Fragments arranged by size:
1753 1619 965 958 429 416 399 310
83 53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 3382 4101 4325 5705 1239
Size: 1166 977 719 224 1380 2579
Fragments arranged by size:
2579 1380 1166 977 719 224
Bpu10I CC'TnA_GC
Cuts at: 2793 2793
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5170 6035 6333 5170
Size: 865 298 5882
Fragments arranged by size:
5882 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3807 5708 882
Size: 617 2308 1901 2219
Fragments arranged by size:
2308 2219 1901 617
BsaAI yAC'GTr
Cuts at: 493 1204 4836 493
Size: 711 3632 2702
Fragments arranged by size:
3632 2702 711
BsaBI GATnn'nnATC
Cuts at: 7039 7039
Size: 7045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2915 4044 4852 5234
Size: 53 83 186 936 1382 1129 808 382
Cuts at: 5234 5869 275
Size: 635 1451
Fragments arranged by size:
1451 1382 1129 936 808 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2135 5478 5876 6150 6297 6708 2135
Size: 3343 398 274 147 411 2472
Fragments arranged by size:
3343 2472 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2235 2265 1178
Size: 30 165 30 832 30 5958
Fragments arranged by size:
5958 832 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 3809 3956 1186
Size: 1276 1347 147 4275
Fragments arranged by size:
4275 1347 1276 147
BseYI C'CCAG_C
Cuts at: 2239 2998 3767 6248 6744 2239
Size: 759 769 2481 496 2540
Fragments arranged by size:
2540 2481 769 759 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2921 3637 1163
Size: 573 1185 716 4571
Fragments arranged by size:
4571 1185 716 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4341 4495 5256 6284 1540
Size: 275 186 2340 154 761 1028 2301
Fragments arranged by size:
2340 2301 1028 761 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2022 3306 3558 4311 5106 5191 6262 2022
Size: 1284 252 753 795 85 1071 2805
Fragments arranged by size:
2805 1284 1071 795 753 252 85
BsmI GAATG_Cn'
Cuts at: 2101 3256 4556 4649 2101
Size: 1155 1300 93 4497
Fragments arranged by size:
4497 1300 1155 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 2777
Size: 7045
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2143 2266 3235 3306 3558 3801
Size: 399 232 121 123 969 71 252 243
Cuts at: 3801 4007 4156 4311 4364 5106 5191 6262 1391
Size: 206 149 155 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 969 742 399 252 243 232
206 155 149 123 121 85 71 53
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 3138 4934 6664 3138
Size: 1796 1730 3519
Fragments arranged by size:
3519 1796 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 3825 4400 844
Size: 1576 1405 575 3489
Fragments arranged by size:
3489 1576 1405 575
BsrBI CCG'CTC
Cuts at: 1394 1823 4932 1394
Size: 429 3109 3507
Fragments arranged by size:
3507 3109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2055 3904 5540 5714 65
Size: 1183 807 1849 1636 174 1396
Fragments arranged by size:
1849 1636 1396 1183 807 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 5689 5876 1700
Size: 110 325 3554 187 2869
Fragments arranged by size:
3554 2869 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 3156 96
Size: 3060 3985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 5105 6117 5105
Size: 1012 6033
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 3023 3064 1184
Size: 1839 41 5165
Fragments arranged by size:
5165 1839 41
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 3088 4039 2038
Size: 1050 951 5044
Fragments arranged by size:
5044 1050 951
BstYI r'GATC_y
Cuts at: 1972 4101 4375 4742 5127 5144 6596 6682 6694
Size: 2129 274 367 385 17 1452 86 12
Cuts at: 6694 7040 1972
Size: 346 1977
Fragments arranged by size:
2129 1977 1452 385 367 346 274 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2761 3087 3750 513
Size: 552 1696 326 663 3808
Fragments arranged by size:
3808 1696 663 552 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6306
Fragments arranged by size:
6306 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2970 4561 5355 5375 2586
Size: 384 1591 794 20 4256
Fragments arranged by size:
4256 1591 794 384 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7010
DraI TTT'AAA
Cuts at: 4349 4349
Size: 7045
DraIII CAC_nnn'GTG
Cuts at: 3211 3211
Size: 7045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3333 3385 4338
Size: 54 1425 50 275 18 1503 52 953
Cuts at: 4338 4492 5381 7010 8
Size: 154 889 1629 43
Fragments arranged by size:
1629 1503 1425 953 889 275 154 54
52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4338 4492 1537
Size: 275 2526 154 4090
Fragments arranged by size:
4090 2526 275 154
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 3425 4975 1353
Size: 657 1415 1550 3423
Fragments arranged by size:
3423 1550 1415 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 5632 6006 6152 387
Size: 1064 138 2525 1518 374 146 1280
Fragments arranged by size:
2525 1518 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3556 3556
Size: 7045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2405 3137 3382 3601 4101 4202
Size: 393 587 186 732 245 219 500 101
Cuts at: 4202 4272 4325 5108 5705 6492 6760 1239
Size: 70 53 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 732 597 587 500 393
268 245 219 186 101 70 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3287 3335 3287
Size: 48 6997
EcoO109I rG'GnC_Cy
Cuts at: 2757 2985 3328 3478 3517 2757
Size: 228 343 150 39 6285
Fragments arranged by size:
6285 343 228 150 39
EcoRI G'AATT_C
Cuts at: 2934 4354 4732 2934
Size: 1420 378 5247
Fragments arranged by size:
5247 1420 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3451 3973 3451
Size: 522 6523
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 4047 6192 1678
Size: 2369 2145 2531
Fragments arranged by size:
2531 2369 2145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 2181 2213 2323
Size: 32 135 32 540 32 37 32 110
Cuts at: 2323 2355 5686 5718 5760 5792 1373
Size: 32 3331 32 42 32 2626
Fragments arranged by size:
3331 2626 540 135 110 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4389 4635 669
Size: 988 281 2451 246 3079
Fragments arranged by size:
3079 2451 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3161 748
Size: 2413 4632
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KasI G'GCGC_C
Cuts at: 4043 4043
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 2496 2802 4644 2496
Size: 306 1842 4897
Fragments arranged by size:
4897 1842 306
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3618 4189 6159 6343 1305
Size: 2313 571 1970 184 2007
Fragments arranged by size:
2313 2007 1970 571 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3335 3387 10
Size: 54 1425 343 1503 52 3668
Fragments arranged by size:
3668 1503 1425 343 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 2786 4037 5003 5362 5521 518
Size: 648 1356 264 1251 966 359 159 2042
Fragments arranged by size:
2042 1356 1251 966 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2239 3686 3767 4461 5138 6286 6531 1606
Size: 633 1447 81 694 677 1148 245 2120
Fragments arranged by size:
2120 1447 1148 694 677 633 245 81
NarI GG'CG_CC
Cuts at: 4044 4044
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 3087 3750 513
Size: 552 2022 663 3808
Fragments arranged by size:
3808 2022 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 2900 3991 4403 5948 1132
Size: 97 1671 1091 412 1545 2229
Fragments arranged by size:
2229 1671 1545 1091 412 97
PasI CC'CwG_GG
Cuts at: 1348 2038 1348
Size: 690 6355
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4171 1108
Size: 3063 3982
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2757 2985 2757
Size: 228 6817
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7045
PsiI TTA'TAA
Cuts at: 4615 4615
Size: 7045
PstI C_TGCA'G
Cuts at: 830 3244 3822 4397 830
Size: 2414 578 575 3478
Fragments arranged by size:
3478 2414 578 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 2239 3686 3767 2239
Size: 1447 81 5517
Fragments arranged by size:
5517 1447 81
RsrII CG'GwC_CG
Cuts at: 4326 4326
Size: 7045
SacI G_AGCT'C
Cuts at: 3558 3558
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SanDI GG'GwC_CC
Cuts at: 2985 2985
Size: 7045
SapI GCTCTTCn'nnn_
Cuts at: 2010 3425 2010
Size: 1415 5630
SbfI CC_TGCA'GG
Cuts at: 3822 4397 3822
Size: 575 6470
ScaI AGT'ACT
Cuts at: 5293 7005 5293
Size: 1712 5333
SexAI A'CCwGG_T
Cuts at: 2316 2316
Size: 7045
SfcI C'TryA_G
Cuts at: 826 1045 3240 3585 3818 4393 5528 6209 6400
Size: 219 2195 345 233 575 1135 681 191
Cuts at: 6400 826
Size: 1471
Fragments arranged by size:
2195 1471 1135 681 575 345 233 219
191
SfoI GGC'GCC
Cuts at: 4045 4045
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 2135 1700
Size: 435 6610
SmaI CCC'GGG
Cuts at: 1872 2879 4372 1872
Size: 1007 1493 4545
Fragments arranged by size:
4545 1493 1007
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5149 6050 6312 820
Size: 197 926 3206 901 262 1553
Fragments arranged by size:
3206 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 2175 1517
Size: 658 6387
StyI C'CwwG_G
Cuts at: 513 1065 2086 3081 3087 3336 3388 3750 4047
Size: 552 1021 995 6 249 52 362 297
Cuts at: 4047 4215 4484 513
Size: 168 269 3074
Fragments arranged by size:
3074 1021 995 552 362 297 269 249
168 52 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2942 3713 5072 5089 5242 5427 5872
Size: 1255 1362 771 1359 17 153 185 445
Cuts at: 5872 325
Size: 1498
Fragments arranged by size:
1498 1362 1359 1255 771 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3156 3933 5291
Size: 274 80 33 51 1443 1179 777 1358
Cuts at: 5291 7003 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1358 1179 777 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 4211 4243 7005 7037 2235
Size: 32 1944 32 2762 32 2243
Fragments arranged by size:
2762 2243 1944 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2715 3757 2715
Size: 1042 6003
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 2877 4370 1870
Size: 1007 1493 4545
Fragments arranged by size:
4545 1493 1007
XmnI GAAnn'nnTTC
Cuts at: 5174 5174
Size: 7045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
SacII SfiI SrfI SwaI