Restriction map for
pFN21AB3230
(Circular) (Six-base) MAPSORT of: pFN21AB3230.seq Check: 7573 from: 1 to: 6592
pFN21AB3230 6592 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2186 2186
Size: 6592
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3783 4402 278
Size: 53 83 186 936 2247 619 2468
Fragments arranged by size:
2468 2247 936 619 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3935 1937
Size: 1998 4594
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2870 3401 4655 6039 6307 1632
Size: 1238 531 1254 1384 268 1917
Fragments arranged by size:
1917 1384 1254 1238 531 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 2638 5491 6468 1203
Size: 1435 2853 977 1327
Fragments arranged by size:
2853 1435 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 5321 5321
Size: 6592
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6592
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 6560
AlwNI CAG_nnn'CTG
Cuts at: 2091 4335 5907 2091
Size: 2244 1572 2776
Fragments arranged by size:
2776 2244 1572
ApaI G_GGCC'C
Cuts at: 2286 2286
Size: 6592
ApaLI G'TGCA_C
Cuts at: 3239 4649 5805 3239
Size: 1410 1156 4026
Fragments arranged by size:
4026 1410 1156
ApoI r'AATT_y
Cuts at: 1493 3901 4127 4279 6388 1493
Size: 2408 226 152 2109 1697
Fragments arranged by size:
2408 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3917 1870
Size: 73 1048 926 4545
Fragments arranged by size:
4545 1048 926 73
BamHI G'GATC_C
Cuts at: 2377 3355 3922 4289 2377
Size: 978 567 367 4680
Fragments arranged by size:
4680 978 567 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3257 3913 5368 618
Size: 325 217 627 1470 656 1455 1842
Fragments arranged by size:
1842 1470 1455 656 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2223 2286 3063 3138 3852 3911 1391
Size: 661 171 63 777 75 714 59 4072
Fragments arranged by size:
4072 777 714 661 171 75 63 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 3335 928
Size: 971 506 930 4185
Fragments arranged by size:
4185 971 930 506
BbvCI CC'TCA_GC
Cuts at: 2198 2198
Size: 6592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4783 4817 1903
Size: 34 2846 34 3678
Fragments arranged by size:
3678 2846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2431 4484 5694 2431
Size: 2053 1210 3329
Fragments arranged by size:
3329 2053 1210
BclI T'GATC_A
Cuts at: 1548 2860 1548
Size: 1312 5280
BfrBI ATG'CAT
Cuts at: 2654 2654
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5203 136
Size: 107 122 71 631 605 3531 1525
Fragments arranged by size:
3531 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 2778 4009 2778
Size: 1231 5361
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2286 3243 4653 5809 1790
Size: 384 112 957 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 957 384 112
BmgBI CAC'GTC
Cuts at: 1318 2660 1318
Size: 1342 5250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3649 3855 4323
Size: 416 53 83 399 24 2232 206 468
Cuts at: 4323 5281 442
Size: 958 1753
Fragments arranged by size:
2232 1753 958 468 416 399 206 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 3077 2923
Size: 154 6438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2066 2621 3122 5252 1239
Size: 797 30 555 501 2130 2579
Fragments arranged by size:
2579 2130 797 555 501 30
Bpu10I CC'TnA_GC
Cuts at: 2198 2198
Size: 6592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 4717 5582 5880 2207
Size: 2510 865 298 2919
Fragments arranged by size:
2919 2510 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 2597 3130 5255 882
Size: 617 795 303 533 2125 2219
Fragments arranged by size:
2219 2125 795 617 533 303
BsaAI yAC'GTr
Cuts at: 493 1204 4383 493
Size: 711 3179 2702
Fragments arranged by size:
3179 2702 711
BsaBI GATnn'nnATC
Cuts at: 6586 6586
Size: 6592
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 3780 4399 4781
Size: 53 83 186 936 792 1455 619 382
Cuts at: 4781 5416 275
Size: 635 1451
Fragments arranged by size:
1455 1451 936 792 635 619 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2500 3155 5025 5423 5697 5844 6255 2500
Size: 655 1870 398 274 147 411 2837
Fragments arranged by size:
2837 1870 655 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6367
Fragments arranged by size:
6367 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 1186
Size: 1510 5082
BseYI C'CCAG_C
Cuts at: 2294 5795 6291 2294
Size: 3501 496 2595
Fragments arranged by size:
3501 2595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3049 3859 1163
Size: 573 1313 810 3896
Fragments arranged by size:
3896 1313 810 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 4803 5831 1540
Size: 275 186 2041 761 1028 2301
Fragments arranged by size:
2301 2041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 3063 3138 3243 4653 4738 5809 2052
Size: 1011 75 105 1410 85 1071 2835
Fragments arranged by size:
2835 1410 1071 1011 105 85 75
BsmI GAATG_Cn'
Cuts at: 4103 4196 4103
Size: 93 6499
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2174 2223 2286 3063 3138 3243
Size: 399 262 122 49 63 777 75 105
Cuts at: 3243 3852 3911 4653 4738 5809 1391
Size: 609 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 777 742 609 399 262 122
105 85 75 63 59 49
BspEI T'CCGG_A
Cuts at: 2500 6255 2500
Size: 3755 2837
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2039 2186 3947 844
Size: 1195 147 1761 3489
Fragments arranged by size:
3489 1761 1195 147
BsrBI CCG'CTC
Cuts at: 1394 1823 2280 2314 2812 3255 4479 1394
Size: 429 457 34 498 443 1224 3507
Fragments arranged by size:
3507 1224 498 457 443 429 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 5087 5261 65
Size: 1183 3839 174 1396
Fragments arranged by size:
3839 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 3507 3889 5236 5423 1700
Size: 110 371 1326 382 1347 187 2869
Fragments arranged by size:
2869 1347 1326 382 371 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2802 3247 794
Size: 2008 445 4139
Fragments arranged by size:
4139 2008 445
BstBI TT'CG_AA
Cuts at: 5396 5396
Size: 6592
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstYI r'GATC_y
Cuts at: 1972 2377 3355 3922 4289 4674 4691 6143 6229
Size: 405 978 567 367 385 17 1452 86
Cuts at: 6229 6241 6587 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 978 567 405 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 4195
Fragments arranged by size:
4195 939 906 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5853
Fragments arranged by size:
5853 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4108 4902 4922 3316
Size: 792 794 20 4986
Fragments arranged by size:
4986 794 792 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3349 3896 3349
Size: 547 6045
DraIII CAC_nnn'GTG
Cuts at: 2129 3819 2129
Size: 1690 4902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3505 4039 4928
Size: 54 1425 50 275 18 1675 534 889
Cuts at: 4928 6557 8
Size: 1629 43
Fragments arranged by size:
1675 1629 1425 889 534 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4039 1537
Size: 275 2227 4090
Fragments arranged by size:
4090 2227 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 3051 3428 4522 1353
Size: 717 981 377 1094 3423
Fragments arranged by size:
3423 1094 981 717 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2332 5179 5553 5699 387
Size: 1064 138 743 2847 374 146 1280
Fragments arranged by size:
2847 1280 1064 743 374 146 138
EcoICRI GAG'CTC
Cuts at: 2050 3061 3136 2050
Size: 1011 75 5506
Fragments arranged by size:
5506 1011 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2066 2621 2870 3122 3401 4655
Size: 393 404 30 555 249 252 279 1254
Cuts at: 4655 5252 6039 6307 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1254 787 597 555 404 393 279
268 252 249 30
EcoO109I rG'GnC_Cy
Cuts at: 2059 2353 2059
Size: 294 6298
EcoRI G'AATT_C
Cuts at: 3901 4279 3901
Size: 378 6214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2572 3286 2485
Size: 87 714 5791
Fragments arranged by size:
5791 714 87
FspI TGC'GCA
Cuts at: 2080 3792 5098 2080
Size: 1712 1306 3574
Fragments arranged by size:
3574 1712 1306
HaeII r_GCGC'y
Cuts at: 1678 3620 5739 1678
Size: 1942 2119 2531
Fragments arranged by size:
2531 2119 1942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3347 3379 3407 3410 3439
Size: 32 135 32 1775 32 28 3 29
Cuts at: 3439 3442 3713 3745 5233 5265 5307 5339 1373
Size: 3 271 32 1488 32 42 32 2626
Fragments arranged by size:
2626 1775 1488 271 135 42 32 32
32 32 32 32 29 28 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2636 3936 4182 669
Size: 988 281 698 1300 246 3079
Fragments arranged by size:
3079 1300 988 698 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5706 5890 1305
Size: 4401 184 2007
Fragments arranged by size:
4401 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4770
Fragments arranged by size:
4770 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3089 3272 4550 4909 5068 518
Size: 648 1375 548 183 1278 359 159 2042
Fragments arranged by size:
2042 1375 1278 648 548 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 3627 3886 4008 4685 5833 6078 1606
Size: 2021 259 122 677 1148 245 2120
Fragments arranged by size:
2120 2021 1148 677 259 245 122
NaeI GCC'GGC
Cuts at: 2183 3509 2183
Size: 1326 5266
NcoI C'CATG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 4195
Fragments arranged by size:
4195 939 906 552
NdeI CA'TA_TG
Cuts at: 387 3076 387
Size: 2689 3903
NgoMIV G'CCGG_C
Cuts at: 2181 3507 2181
Size: 1326 5266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NsiI A_TGCA'T
Cuts at: 2656 2656
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 2189 2642 3950 5495 1132
Size: 97 960 453 1308 1545 2229
Fragments arranged by size:
2229 1545 1308 960 453 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6592
PciI A'CATG_T
Cuts at: 2638 5491 2638
Size: 2853 3739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6592
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2353 2059
Size: 294 6298
PshAI GACnn'nnGTC
Cuts at: 1613 2405 1613
Size: 792 5800
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PspOMI G'GGCC_C
Cuts at: 2282 2282
Size: 6592
PstI C_TGCA'G
Cuts at: 830 3252 3944 830
Size: 2422 692 3478
Fragments arranged by size:
3478 2422 692
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 3886 3886
Size: 6592
SacI G_AGCT'C
Cuts at: 2052 3063 3138 2052
Size: 1011 75 5506
Fragments arranged by size:
5506 1011 75
SalI G'TCGA_C
Cuts at: 1936 3934 1936
Size: 1998 4594
SapI GCTCTTCn'nnn_
Cuts at: 3051 3051
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 4840 6552 4840
Size: 1712 4880
SfcI C'TryA_G
Cuts at: 826 1045 2240 2876 3248 3940 5075 5756 5947
Size: 219 1195 636 372 692 1135 681 191
Cuts at: 5947 826
Size: 1471
Fragments arranged by size:
1471 1195 1135 692 681 636 372 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3919 1872
Size: 2047 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 4696 5597 5859 820
Size: 197 926 279 2474 901 262 1553
Fragments arranged by size:
2474 1553 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 4383 493
Size: 3890 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3950 1132
Size: 2818 3774
SspI AAT'ATT
Cuts at: 5 52 4516 5
Size: 47 4464 2081
Fragments arranged by size:
4464 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6592
StyI C'CwwG_G
Cuts at: 513 1065 2004 2910 4031 513
Size: 552 939 906 1121 3074
Fragments arranged by size:
3074 1121 939 906 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3631 4619 4636 4789 4974 5419 325
Size: 1255 2051 988 17 153 185 445 1498
Fragments arranged by size:
2051 1498 1255 988 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3141 3264 4838
Size: 274 80 33 51 1443 1164 123 1574
Cuts at: 4838 6550 96
Size: 1712 138
Fragments arranged by size:
1712 1574 1443 1164 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6552 6584 6552
Size: 32 6560
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 2212 3928 2212
Size: 1716 4876
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2143 3470 2011
Size: 132 1327 5133
Fragments arranged by size:
5133 1327 132
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3917 1870
Size: 2047 4545
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3781 4400 276
Size: 53 83 186 936 2247 619 2468
Fragments arranged by size:
2468 2247 936 619 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI